Prnd (NM_001278257) Mouse Untagged Clone

SKU
MC226100
Prnd (untagged) - Mouse prion protein dublet (Prnd), transcript variant 3
  $330.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Prnd
Synonyms AI450264; doppel; Dpl; PrPLP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC226100 representing NM_001278257
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAACCGGCTGGGTACATGGTGGGTGGCCATCCTCTGCATGCTGCTTGCCAGCCACCTCTCCACGG
TCAAGGCAAGGGGCATAAAGCACAGGTTCAAGTGGAACCGGAAGGTCCTGCCCAGCAGCGGCGGCCAGAT
CACCGAAGCTCGGGTAGCTGAGAACCGCCCAGGAGCCTTCATCAAGCAAGGCCGGAAGCTGGACATCGAC
TTTGGAGCAGAGGGCAACAGGTACTACGCGGCTAACTATTGGCAGTTCCCTGATGGGATCTACTACGAAG
GCTGCTCTGAAGCCAACGTGACCAAGGAGATGCTGGTGACCAGCTGCGTCAACGCCACCCAGGCGGCCAA
CCAGGCTGAGTTCTCCCGGGAGAAGCAGGATAGCAAGCTCCACCAGCGAGTCCTGTGGCGGCTGATCAAA
GAGATCTGCTCCGCCAAGCACTGCGATTTCTGGCTGGAAAGGGGAGCTGCGCTTCGGGTCGCCGTGGACC
AACCGGCGATGGTCTGCCTGCTGGGTTTCGTTTGGTTCATTGTGAAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001278257
Insert Size 540 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001278257.1, NP_001265186.1
RefSeq Size 1970 bp
RefSeq ORF 540 bp
Locus ID 26434
UniProt ID Q9QUG3
Cytogenetics 2 64.07 cM
Summary Required for normal acrosome reaction and for normal male fertility (PubMed:12110578, PubMed:15161660, PubMed:15007175). Can bind Cu(2+) (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) differs in the 3' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein.
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Documents
Resources
"NM_001278257" in other vectors (1)
SKU Description Size Price
MR228311 Prnd (myc-DDK-tagged) - Mouse prion protein dublet (Prnd), transcript variant 3 10 ug
$289.00 $300.00

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