Fuom (NM_001286217) Mouse Untagged Clone

CAT#: MC226006

Fuom (untagged) - Mouse fucose mutarotase (Fuom), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fuom
• Synonyms: Fucu; Le51
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226006 representing NM_001286217
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGCGCTCAAGGGCATCCCGAAGGTGCTGTCTCCTGAGCTGTTGTTCGCGCTTGCGCGGATGGGGC
  ATGGAGACGAAATTGTCCTTGCTGATGCGAACTTCCCTACCTCCTCCATCTGCCAGTGCGGACCTGTGGA
  GATCCGAGCAGACGGCCTGGACATCCCACAGCTTCTGGAGGCTGTGCTGAGGCTCCTGCCCCTGGACACC
  TACGTGGAAAGCCCGGCTGCTGTCATGGACCTGGTGCCCAGTGACAAGGAGAAGGGCCTGCAGACCCCGA
  TATGGAAGCGTTATGAATCCCTTCTTCTCGAAGCTGACTGTAAAAAAACCCTGATGAAGCTAGAGAGATT
  TGAATTTTATGAACGTGCAAAAAAGGCATTTGCTGTGGTTGCAACCGGCAACCAGACCTGGACATCAGCC
  TCGGGCTCAAGAAGATGGCAGATCTTGAGAGACTCTGCTGACCCTGACAAATTCCCCCTTCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286217
• Insert Size: 486 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286217.1, NP_001273146.1
• RefSeq Size: 2256 bp
• RefSeq ORF: 486 bp
• Locus ID: 69064
• UniProt ID: Q8R2K1
• Cytogenetics: 7 F4
• Gene Summary: Involved in the interconversion between alpha- and beta-L-fucoses. L-Fucose (6-deoxy-L-galactose) exists as alpha-L-fucose (29.5%) and beta-L-fucose (70.5%), the beta-form is metabolized through the salvage pathway. GDP-L-fucose formed either by the de novo or salvage pathways is transported into the endoplasmic reticulum, where it serves as a substrate for N- and O-glycosylations by fucosyltransferases. Fucosylated structures expressed on cell surfaces or secreted in biological fluids are believed to play a critical role in cell-cell adhesion and recognition processes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3'-terminal exon, compared to variant 1. The encoded isoform (2) has a longer and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.