Lmo1 (NM_001302205) Mouse Untagged Clone

CAT#: MC225964

Lmo1 (untagged) - Mouse LIM domain only 1 (Lmo1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lmo1
• Synonyms: Rbtn-1; Rbtn1; Ttg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225964 representing NM_001302205
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTGGACCAAGAGGACGGTGTGCCGATGCTCTCCGTCCAACCTAAGGGGAAACAGAAGGGCTGCG
  CAGGCTGCAACCGCAAGATCAAGGACCGGTACCTGCTGAAGGCACTGGACAAGTACTGGCATGAGGACTG
  CCTCAAGTGTGCCTGCTGTGACTGTCGCCTGGGCGAGGTGGGCTCCACTCTCTACACCAAGGCCAACCTC
  ATCCTGTGCCGGCGTGACTACCTGAGGCTTTTTGGCACCACAGGAAACTGTGCTGCCTGCAGCAAGCTGA
  TCCCAGCTTTTGAGATGGTGATGCGGGCCCGAGACAATGTGTATCACCTTGACTGCTTCGCCTGCCAGCT
  CTGCAATCAGAGATTTTGCGTGGGAGACAAATTCTTCCTGAAGAACAACATGATCTTGTGCCAGGTGGAC
  TATGAGGAGGGGCATCTCAATGGCACCTTTGAATCCCAGGTTCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302205
• Insert Size: 468 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302205.1, NP_001289134.1
• RefSeq Size: 1022 bp
• RefSeq ORF: 468 bp
• Locus ID: 109594
• UniProt ID: Q924W9
• Cytogenetics: 7 57.21 cM
• Gene Summary: May be involved in gene regulation within neural lineage cells potentially by direct DNA binding or by binding to other transcription factors.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an upstream alternate 5'-terminal exon which reflects use of an alternate promoter supported by epigenetic data, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus compared to isoform 1.