Gm44501 (NM_001287195) Mouse Untagged Clone

CAT#: MC225932

Esp6Esp5 (untagged) - Mouse Esp6-Esp5 readthrough transcript (Esp6Esp5)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gm44501
• Synonyms: Esp6-esp5; Esp6Esp5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225932 representing NM_001287195
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCCTTTACCAGTGATGTTTTTTCTCCTAACTCTGCTGTTACCATCAATGCACACTGATGGAAGGG
  TACTGACACAGACTCAAAATGAATCCACCAAATCTTCTAATCATAAGAAAAATCACAAGGCTGTCTTGGA
  CAAGACTGACCGCCAAGACAAAGGAAATAAACAGGCCCCAGAGAAAGTATTCTGTGCCTCCATTGATGAT
  CAAACATTAGTTGAAGACTTGGCCACTGCTAATCAACACAAGTTAATCCTGTCTAAAAACAAGATATCCC
  AGAGCAACTGTTGTACTCAGAAGCATCAAGTGGACCCAGTAAACTTGAATCATATGGCCAGTAGACTTCA
  AAGGACCAGAGTGAGGCACCAAGAAGACAGTTCTAAACGCAAAGTCATCGTGGGTTTGGATGAATTCTTG
  GATTTAGTGAAAAAGATCTTGATTTCCCATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287195
• Insert Size: 453 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287195.1, NP_001274124.1
• RefSeq Size: 1229 bp
• RefSeq ORF: 453 bp
• Locus ID: 102577428
• Cytogenetics: 17
• Gene Summary: This locus represents naturally occurring readthrough transcription between the neighboring Esp6 (exocrine gland secreted peptide 6) and Esp5 (exocrine gland secreted peptide 5) genes on chromosome 17. The readthrough transcript encodes a protein that shares sequence identity with the downstream gene product, but is shorter at its N-terminus. [provided by RefSeq, Dec 2013]