Pkig (NM_001039391) Mouse Untagged Clone

SKU
MC225521
Pkig (untagged) - Mouse protein kinase inhibitor, gamma (Pkig), transcript variant 3
  $165.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pkig
Synonyms PKIgamma
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC225521 representing NM_001039391
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAAGTCGAGTCCTCCTACTCGGACTTCATCTCCTGCGACCGGACAGGCCGTCGGAATGCAGTCC
CTGACATCCAGGGTGACTCGGAGGCCGTGAGTGTGCGGAAGCTCGCTGGAGACATGGGCGAGCTCGCACT
TGAGGGAGCAGAAGGACAGGCAGAGGGAAGTACCCCCGACAAGGAAGCCAGCAGCCAGCCTGAGAGCAGT
GATGCGAACACCTCATCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001039391
Insert Size 231 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039391.2, NP_001034480.1
RefSeq Size 1053 bp
RefSeq ORF 231 bp
Locus ID 18769
UniProt ID O70139
Cytogenetics 2 84.4 cM
Summary Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3, 4, and 5 encode the same isoform (1).
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Documents
Resources
"NM_001039391" in other vectors (1)
SKU Description Size Price
MR227732 Pkig (myc-DDK-tagged) - Mouse protein kinase inhibitor, gamma (Pkig), transcript variant 3 10 ug
$289.00

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