Atm (NM_007499) Mouse Untagged Clone

CAT#: MC225456

Atm (untagged) - Mouse ataxia telangiectasia mutated homolog (human) (Atm), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atm
• Synonyms: AI256621; C030026E19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225456 representing NM_007499
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTAGCACTCAATGATCTGCTCATTTGCTGCCGGCAGTTAGAGCATGACAGAGCTACAGAAAGAA
  GGAAAGAAGTGGATAAATTTAAGCGCCTGATTCAGGATCCTGAAACAGTTCAACATTTAGATAGGCATTC
  TGATTCCAAACAAGGAAAATATCTGAATTGGGATGCTGTTTTCAGGTTTTTACAGAAGTACATTCAAAAA
  GAAATGGAAAGTCTGAGAACAGCAAAATCAAATGTATCAGCCACCACACAGAGCTCCAGACAGAAGAAGA
  TGCAAGAGATCAGCAGTTTGGTCAGATACTTCATCAAATGTGCAAACAAAAGAGCACCCAGGCTAAAATG
  TCAAGACCTCTTGAATTATGTCATGGATACAGTGAAAGACTCATCTAATGGTCTAACGTATGGAGCTGAC
  TGTAGCAACATACTACTCAAAGACATTCTTTCTGTGAGAAAATACTGGTGTGAAGTATCTCAGCAACAGT
  GGCTAGAATTGTTTTCACTGTACTTCAGGCTGTATCTCAAGCCATCACAGGACATTAATAGAGTTTTAGT
  GGCTAGAATAATTCATGCTGTCACCAGAGGATGCTGTTCACAGACTGATGGATTACCTTCAAAGTTTTTA
  GATCTTTTTTCCAAGGCTATTCAGTATGCCAGACAAGAAAAGAGCTCTCCTGGCTTAAGTCACATCTTAG
  CAGCCCTTAACATTTTCCTCAAGTCTCTGGCTGTCAACTTCCGAAAACGGGTGTGTGAAGCAGGAGATGA
  AATTCTTCCTACCTTACTATATATTTGGACTCAACATAGACTTAATGATTCTTTAAAAGAAGTAATTATT
  GAACTAATTCAACTGCAGATTTATATCCATCATCCACAAGGAGCCAGAGCTCCTGAAGAAGGTGCTTATG
  AATCCATGAAATGGAAAAGTATCTTGTACAACTTATATGACTTGCTAGTGAATGAGATAAGTCATATAGG
  AAGCCGAGGGAAATATTCCTCAGGATCTCGTAATATTGCTGTCAAGGAAAATCTGATTGACCTGATGGCA
  GATATCTGTTACCAGCTTTTTGATGCAGATACCAGATCCGTGGAGATTTCTCAATCTTATGTGACACAAA
  GGGAATCCACTGATTACAGTGTACCTTGCAAAAGAAGGAAAATAGACGTAGGCTGGGAAGTGATAAAAGA
  TTATCTTCAGAAGTCACAGAGTGATTTTGATCTCGTGCCTTGGCTACAGATTACAACCCGATTAATATCA
  AAATATCCTTCCAGTTTACCTAACTGTGAGCTGTCTCCATTAATACTGATACTGTACCAGCTTCTGCCTC
  AACAGCGACGTGGAGAACGCATCCCATATGTGTTACGATGCCTTAAGGAAGTTGCCTTATGTCAAGGCAA
  GAAATCAAACCTGGAAAGCTCTCAGAAGTCAGATTTATTGAAACTATGGATCAAAATTTGGTCTATTACC
  TTTCGTGGTATAAGTTCTGGACAAACACAAACTGAAAACTTTGGTTTACTTGAGGCCATCATTCAAGGTA
  GTTTAGTTGAACTTGACAGAGAATTCTGGAAGTTATTTACTGGCTCAGCCTGTAAACCTTCTAGTCCTTC
  AGTATGCTGCTTGACTTTGGCACTTAGCATCTGTGTAGTTCCAGATGCAATAAAAATGGGAACAGAACAA
  AGTGTGTGTGAAGCAAATAGAAGTTTTTCTGTAAAGGAGTCAATAATGAGGTGGCTCTTATTCTACCAGT
  TAGAGGATGACTTAGAAGACAGCACAGAGCTGCCTCCAATTCTTCAGAGTAATTTTCCTCATCTTGTAGT
  CGAAAAAATTCTTGTAAGTCTCACTATGAAAAACTCAAAAGCTGCAATGAAGTTTTTTCAAAGTGTGCCA
  GAATGTGAACAACACTGCGAAGATAAAGAAGAGCCTTCATTTTCAGAAGTAGAAGAACTGTTTCTTCAGA
  CTACTTTTGACAAGATGGATTTTTTAACTACTGTCAAAGAGTATGCTGTAGAAAAATTTCAGTCTAGTGT
  TGGCTTCTCTGTCCAGCAAAATCTCAAGGAATCATTGGATCACTATCTTCTGGGATTATCAGAACAGCTT
  TTAAGTAATTACTCTTCTGAGATTACAAGTTCTGAAACCCTTGTCCGGTGTTCAAGTCTTTTGGTGGGTG
  TTCTTGGCTGCTATTGTTACATGGGTATAATAACTGAAGACGAAGCCCATAAATCAGAATTATTCCAGAA
  AGCCAAGTCTCTGATGCAATGTGCAGGAGAAAGTATCTCTCTGTTTAAAAATAAAACAAATGAGGAATCA
  AGAATTGGTTCATTGAGAAATGTGATGCATCTGTGTACAAGTTGCTTGTGTATACATACCAAGCATACGC
  CAAACAAGATTGCCTCTGGCTTTTTCCTACGATTATTAACATCAAAGCTTATGAATGACATTGCAGATAT
  TTGTAAAAGTTTAGCATCCTGTACGAAAAAGCCATTGGATCACGGAGTACATCCAGGGGAAGATGATGAA
  GATGGTGGTGGTTGTGACAGTCTGATGGAGGCAGAGGGTCCATCGTCCACTGGTCTTTCTACTGCTTACC
  CCGCTAGTTCTGTGAGCGATGCAAATGATTATGGAGAGAACCAGAATGCTGTTGGTGCCATGAGTCCTTT
  AGCTGCCGACTACCTGTCCAAACAAGATCATCTTCTCTTAGACATGCTCAGGTTCTTAGGCCGATCTGTA
  ACTGCATCTCAGAGCCATACTGTGTCGTTTAGAGGAGCTGACATTAGAAGAAAATTGTTACTGTTGCTTG
  ATTCTAGCATACTCGATCTCATGAAGCCCCTCCACCTGCATATGTACTTAGTGCTCCTGAAGGATCTCCC
  TGGAAACGAGCACTCATTGCCAATGGAAGATGTTGTTGAACTTCTGCAACCATTATCCCTTGTGTGTTCT
  CTGCACCGACGTGACCAAGATGTCTGTAAAACGATTCTAAGCAATGTCCTTCATATAGTGACAAACCTAG
  GCCAGGGCAGTGTGGACATGGAGAGCACACGGATTGCTCAAGGACACTTCCTGACAGTGATGGGAGCATT
  TTGGCATTTGACAAAGGAAAAGAAATGTGTATTCTCTGTAAGAATGGCATTAGTAAAGTGTCTTCAAACA
  TTGCTTGAGGCTGATCCATATTCCGAATGGGCAATTCTTAATGTAAAAGGACAAGACTTTCCTGTAAATG
  AAGCTTTTTCACAATTTCTTGCTGACGATCATCATCAAGTTCGGATGTTGGCTGCAGGGTCAGTCAACAG
  ATTATTTCAGGATATGAGACAAGGCGATTTCTCCAGAAGCTTGAAAGCACTCCCTCTGAAGTTTCAGCAG
  ACATCTTTTAACAATGCATACACGACAGCAGAGGCGGGGATCAGAGGACTGTTATGTGATTCTCAGAACC
  CTGATCTGCTGGATGAGATCTATAACAGAAAATCTGTACTACTGATGATGATAGCTGTGGTCTTGCACTG
  TAGCCCAGTCTGTGAAAAGCAGGCTTTGTTTGCTTTATGCAAGTCTGTGAAGGAAAACAGACTAGAACCT
  CATCTTGTGAAAAAGGTTTTAGAGAAAGTCTCCGAATCGTTTGGATGTAGAAGTTTAGAAGACTTCATGA
  TTTCTCACCTAGACTACCTGGTTTTGGAATGGCTGAACCTTCAAGATACTGAATATAGCTTATCTTCTTT
  TCCTTTTATGTTATTAAACTACACAAGCATTGAGGATTTCTATCGGTCTTGTTACAAGATTTTGATCCCA
  CATTTGGTAATCAGAAGCCATTTTGATGAGGTGAAGTCCATTGCTAATCAGATTCAAAAGTGCTGGAAAA
  GCCTGTTGGTAGATTGCTTTCCGAAGATTCTTGTGCACATCCTTCCTTACTTTGCCTACGAGGGCACGAG
  AGACAGCTACGTGTCACAGAAAAGAGAGACTGCTACCAAGGTCTACGATACTCTTAAAGGGGAAGACTTC
  CTAGGAAAACAGATTGACCAAGTATTCATTAGTAATTTGCCAGAGATTGTGGTGGAGTTGCTGATGACAT
  TGCATGAGACAGCTGACTCGGCTGACTCGGACGCCAGTCAAAGCGCCACCGCCTTGTGTGATTTTTCAGG
  GGATTTGGATCCTGCCCCCAACCCGCCATATTTCCCCTCACATGTCATTCAGGCAACGTTTGCTTACATC
  AGCAACTGTCATAAAACCAAGTTTAAAAGCATTCTAGAAATTCTTTCTAAAATCCCCGATTCCTATCAGA
  AAATACTTCTGGCCATTTGTGAACAAGCAGCTGAGACAAATAATGTCTTTAAAAAGCACAGAATTCTTAA
  AATATATCACCTGTTTGTTAGTTTATTACTGAAAGATATACAGAGTGGCCTGGGAGGGGCTTGGGCCTTT
  GTCCTTCGCGATGTTATTTATACTCTGATTCACTACATCAACAAAAGGTCTTCTCATTTCACAGATGTGT
  CGTTGCGTAGCTTTTCCCTTTGCTGTGACCTATTAAGTCGAGTTTGTCATACAGCTGTAACTCAATGTAA
  GGATGCTCTAGAAAGCCATCTTCACGTTATCGTTGGCACACTTATTCCCCTTGTGGATTATCAGGAAGTT
  CAAGAACAGGTATTGGACCTGTTGAAGTACTTAGTGATAGATAACAAAGACAATAAAAACCTCTCTGTCA
  CAATTAAGCTTTTGGATCCCTTTCCTGACCATGTTATTTTTAAGGACTTGCGTCTTACTCAACAGAAAAT
  CAAATATAGTGGAGGACCTTTTTCACTCTTAGAGGAAATAAACCATTTTCTCTCAGTAAGTGCTTACAAT
  CCACTTCCGCTGACCAGGCTTGAAGGACTGAAGGATCTTCGAAGACAACTGGAGCAACATAAAGATCAGA
  TGCTAGATCTTCTGAGAGCGTCTCAAGATAACCCACAAGATGGCATTGTGGTGAAGCTAGTTGTCAGCTT
  GTTGCAGTTATCCAAGATGGCAGTGAACCAGACTGGTGAAAGAGAAGTTTTAGAGGCTGTCGGAAGGTGT
  TTGGGAGAAATAGGTCCTCTGGATTTCTCCACCATAGCTGTCCAGCATAACAAAGATGTGTCCTATACCA
  AAGCCTACGGGTTACCTGAAGACAGAGAACTTCAGTGGACCTTGATAATGCTGACTGCCCTCAACAATAC
  CCTGGTAGAGGACAGTGTCAAAATTCGATCTGCTGCTGCTACCTGTTTGAAAAACATTTTGGCTACAAAG
  ATTGGACATATTTTCTGGGAGAATTATAAGACATCAGCGGATCCAATGCTGACCTATCTACAACCTTTTA
  GAACATCGAGGAAAAAGTTTTTAGAAGTGCCCCGATCTGTTAAAGAAGATGTTTTAGAAGGCCTGGATGC
  TGTGAATCTGTGGGTTCCTCAAAGTGAAAGTCATGACATTTGGATAAAGACACTGACGTGTGCCTTTCTG
  GACAGTGGAGGCATAAACAGTGAAATTCTCCAGTTATTAAAGCCAATGTGTGAAGTGAAAACCGACTTCT
  GTCAGATGTTGCTGCCATACTTGATCCATGATGTTTTACTGCAAGATACACATGAATCGTGGAGAACTCT
  GCTGTCTGCGCACGTCCGAGGATTTTTCACTAGTTGTTTTAAGCATTCCTCCCAAGCAAGCCGCTCAGCA
  ACTCCTGCAAATTCGGATTCAGAGTCAGAGAACTTTCTCCGATGCTGTTTGGATAAAAAGTCACAAAGAA
  CCATGCTTGCTGTTGTCGACTATCTGAGAAGGCAAAAGAGACCTTCCTCGGGAACAGCTTTTGATGACGC
  TTTCTGGCTGGATTTGAATTATCTTGAGGTTGCGAAGGTGGCTCAGTCCTGCTCTGCTCACTTCACGGCC
  TTGCTCTACGCAGAGATCTATTCAGATAAGAAAAGCACAGACGAGCAAGAGAAAAGAAGTCCAACATTTG
  AAGAAGGAAGTCAAGGAACAACTATTTCTAGTTTGAGTGAAAAAAGTAAAGAAGAAACTGGAATAAGCTT
  ACAGGATCTTCTCTTAGAGATCTACAGAAGTATAGGAGAGCCGGACAGCCTGTATGGCTGTGGAGGAGGG
  AAAATGTTACAACCCCTTACTAGAATACGGACATATGAACATGAAGCTACGTGGGAGAAAGCCTTAGTAA
  CTTACGACCTGGAGACCAGCATCTCCTCCTCCACCCGCCAGTCAGGAATCATCCAGGCCCTGCAGAATTT
  GGGGCTCTCCCATATCCTGTCTGTCTATCTGAAAGGATTAGACTATGAAAGACGAGAGTGGTGCGCTGAG
  CTGCAGGAGCTGCGTTACCAGGCGGCGTGGAGGAACATGCAGTGGGGCCTCTGCGCTTCTGCCGGCCAAG
  AAGTAGAAGGAACCAGTTACCATGAATCGTTGTATAATGCTCTGCAGTGTCTAAGAAACAGAGAATTCTC
  CACATTTTATGAAAGTCTCCGATATGCCAGTCTTTTCAGGGTGAAAGAAGTTGAAGAGTTGAGTAAGGGC
  AGCCTTGAGTCTGTATATTCGCTGTATCCCACACTTAGTAGATTGCAGGCAATTGGAGAACTGGAAAACA
  GTGGCGAGCTTTTCTCAAGGTCAGTCACAGACAGAGAGCGCTCTGAAGCATACTGGAAGTGGCAGAAGCA
  CTCCCAGCTTCTGAAAGACAGCGACTTCAGCTTTCAGGAGCCTCTCATGGCTCTGCGCACAGTCATTCTG
  GAGACCCTGGTACAGAAGGAAATGGAGCGCTCTCAAGGAGCATGCTCTAAGGACATTCTCACCAAACACC
  TCGTTGAATTCTCTGTTCTGGCTCGAACCTTCAAGAACACACAGCTCCCTGAAAGAGCAATATTCAAAAT
  TAAGCAATATAATTCAGCTATTTGTGGAATTTCTGAGTGGCATTTGGAAGAAGCACAAGTATTCTGGGCA
  AAAAAGGAGCAGAGTCTTGCTCTGAGTATTCTCAAGCAGATGATCAAGAAGTTGGACTCCAGCTTTAAAG
  ATAAAGAGAATGATGCAGGTCTCAAAGTCATATACGCAGAGTGTCTGAGGGTTTGTGGCAGCTGGCTGGC
  AGAAACTTGCTTAGAAAACCCTGCAGTCATCATGCAGACCTATCTAGAAAAGGCGGTGAAGGTTGCTGGA
  AGTTACGATGGCAACAGCAGAGAGCTCAGAAATGGACAGATGAAGGCCTTTCTCTCGTTGGCAAGGTTCT
  CTGATACTCAGTACCAGAGAATTGAAAACTACATGAAGTCATCAGAATTTGAAAACAAGCAAACTCTCTT
  AAAAAGAGCCAAAGAGGAAGTGGGCCTTCTAAGGGAACATAAAATTCAGACCAACAGATACACAGTAAAG
  GTTCAGCGAGAACTGGAGCTGGACGAATGTGCTCTCCGTGCACTGAGAGAGGATCGCAAGCGCTTCCTGT
  GTAAAGCAGTGGAGAACTACATCAACTGCTTACTAAGCGGGGAAGAACATGATCTGTGGGTGTTCCGGCT
  TTGCTCCCTCTGGCTTGAAAATTCTGGAGTTTCTGAAGTCAATGGCATGATGAAGAAAGATGGAATGAAG
  ATTTCATCCTATAAGTTTTTGCCTCTCATGTATCAATTGGCTGCTCGAATGGGGACCAAAATGACGGGAG
  GCCTAGGATTTCACGAAGTCCTCAATAATCTAATCTCTAGGATTTCACTGGATCACCCCCATCATACTTT
  GTTCATTATACTGGCCTTAGCAAATGCGAACAAAGATGAATTTTTGAGCAAACCAGAGACAACAAGAAGG
  AGTCGAATAACCAAAAGTACATCTAAAGAAAACTCTCACCTTGATGAGGATCGAACAGAGGCTGCAACCA
  GAATCATCCACTCCATCAGAAGTAAGCGATGTAAGATGGTGAAGGACATGGAGGCGCTCTGCGATGCCTA
  CATCATCTTGGCAAACATGGACGCCTCTCAGTGGAGGGCTCAGAGAAAAGGCATCAATATTCCAGCCAAC
  CAGCCAATCACTAAACTGAAGAATTTAGAAGATGTTGTTGTTCCCACTATGGAAATTAAGGTTGATCCCA
  CAGGAGAGTATGAAAATCTGGTGACTATAAAATCATTTAAAACAGAATTTCGCTTAGCTGGAGGCTTAAA
  TTTACCCAAAATAATAGATTGTGTGGGTTCTGATGGCAAGGAAAGGAGACAGCTTGTGAAGGGCCGTGAT
  GACCTGAGGCAAGATGCTGTCATGCAGCAGGTCTTCCAGATGTGCAATACACTACTGCAGAGAAACACTG
  AGACTAGAAAGAGGAAACTGACTATCTGCACATACAAGGTGGTTCCCCTTTCTCAGCGAAGCGGTGTTCT
  CGAGTGGTGCACAGGAACCGTTCCTATTGGTGAATATCTTGTTAACAGCGAAGACGGTGCACATAGAAGA
  TACAGGCCAAATGATTTCAGTGCCAATCAGTGCCAAAAGAAAATGATGGAAGTGCAGAAGAAGTCTTTTG
  AAGAGAAATATGATACCTTCATGACGATTTGCCAAAACTTTGAACCAGTTTTCCGTTACTTCTGCATGGA
  AAAATTCTTGGACCCAGCTGTTTGGTTTGAGAAACGATTGGCATATACACGCAGTGTGGCCACATCTTCT
  ATCGTCGGTTACATCCTTGGACTTGGCGACAGGCACGTACAGAATATCTTGATAAACGAGCAGTCGGCAG
  AGCTTGTGCACATAGACCTGGGAGTGGCTTTTGAACAGGGGAAGATCCTTCCCACTCCAGAAACAGTTCC
  TTTTAGACTCAGCAGAGATATTGTGGACGGGATGGGCATCACCGGTGTGGAAGGTGTCTTCAGAAGGTGC
  TGTGAAAAAACGATGGAAGTTATGCGGAGTTCTCAGGAAACCCTGCTGACCATTGTAGAGGTTCTTTTGT
  ACGATCCACTCTTTGATTGGACTATGAATCCTTTAAAAGCTCTGTATCTACAGCAGAGACCAGAAGATGA
  GTCCGACCTCCATTCCACCCCCAATGCAGATGATCAAGAATGCAAACAAAGTCTTAGTGATACTGACCAG
  AGTTTCAACAAAGTAGCTGAGCGTGTCTTGATGAGACTGCAAGAGAAACTGAAAGGCGTGGAGGAAGGCA
  CTGTGCTCAGTGTGGGTGGACAGGTGAACTTGCTTATCCAGCAGGCCATGGATCCCAAAAATCTCAGCCG
  ACTCTTCCCAGGATGGAAAGCTTGGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007499
• Insert Size: 9201 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007499.2, NP_031525.2
• RefSeq Size: 11964 bp
• RefSeq ORF: 9192 bp
• Locus ID: 11920
• UniProt ID: Q62388
• Cytogenetics: 9 29.12 cM
• Gene Summary: Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Binds DNA ends. Plays a role in replication-dependent histone mRNA degradation. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.[UniProtKB/Swiss-Prot Function]