Ep400 (NM_173066) Mouse Untagged Clone

CAT#: MC225440

Ep400 (untagged) - Mouse E1A binding protein p400 (Ep400), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ep400
• Synonyms: 1700020J09Rik; AU023439; mDomino; mKIAA1498; p400
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225440 representing NM_173066
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCATGGCAGTGGTCCTCAGAATGTCCAACATCAGCTGCAGAGGTCTAGGTCCTTCACTGGGAGTG
  AAGAGGAGCAGCCAGCCCATCCCAACTTACCCCCATCTCCTGCAGCGCCTTTCGCTCCATCAGCCAGTCC
  ATCTGCACCCCAATCCCCTGGGTACCAGATACAGCAGCTGATGAGCAGAAGTCCTGTGGCCGGGCAGAAT
  GTGAACATCACACTGCAGAATGTTGGCCCAGTTGTGGGAGGAAACCAGCAGATCACACTGGCCCCCCTGC
  CACTGCCCAACCCCACTTCTCCAGGTTTTCAGTTTGGTGCCCAGCAAAGGCGCTTTGAGCATGGCTCTCC
  ATCATACATTCAAGTTACTTCTCCGATGTCGCAGCAGGTCCAGACTCAAAGCCCCACACAGCCTAGCCCT
  GGGCCAGGGCAAACCCTGCAGAATGTGCGTGCAGGTGCTCCAGGTCCTGGGCTGGGCATATGCAGCAACA
  GCCCCACTGGAGGCTTTGTGGATGCCAGTGTGCTTGTGAGGCAGATCAGCTTGAGTCCATCAAGTGGTGG
  TCACTTTGTATTTCAGGAGGCACCAGGCCTGACTCAGATGGCCCAGGGAGCCCAGGTTCAACTCCAGCAT
  TCAGGAGCTCCCATCACTGTCCGAGAACGGAGACTCTCCCAACCTCATGCACAGTCGGGAGGCACCATCC
  ACCATCTAGGGCCTCAGAGCCCTGCAGCTGCAGGAGGCACTGGACTTCAACCTCTGGCCAGCCCAAACCA
  CATCACCACGGCCAGCCTGCCACCCCAGATCAGCAGCATTATTCAGGGCCAGTTGATACAGCAACAGCAG
  CAGGTGCTTCAGGGACAGCCAATGAACCGATCTCTGGGATTTGAGAGGACACCTGGTGTGCTGCTTCCTG
  GCGTGGGAGGGCCTTCAGCATTTGGGATGACATCCCCACCACCACCCACCAGCCCTTCCAGAACCACCAT
  GCCTCCAGGCCTTTCCAGTGTGCCTCTTACATCTATGGGAAGCTCAGGAATGAAGAAGGTTCCCAAGAAG
  TTGGAGGAGATCCCCCCAGCCTCCCAAGAGATGGCACAGATGAGGAAGCAGTGTCTGGACTACCACTACA
  AGGAAATGGAGGCACTCAAGGAAGTCTTCAAGGAGTACCTGATTGAACTGTTCTTCTTGCAACATCTACA
  GGGAAACATGATGGATTTCTTGGCTTTTAAAAAGAAGCACTATGCCCCTTTACAAGCGTATCTTAGGCAG
  AATGATTTGGACATTGAAGAGGAGGAGGAGGAAGAGGAAGAGGAGGAAGGAAAATCTGAAGTTATCAACG
  ATGAGCACCAATCCCTGACTGGGAGCCTAGTGGTGGGGCCTGGCAGTGCCACAGAAGCCGACCCCTTTAA
  GAGGCAACAGGTGATGCCACCCACAGGAGGGATGCCCCCAACTCCTCAGGCCACACAGCTCACTGGACAG
  AAGCAGAGTCAGCAGCAGTACGACCCTTCCACAGGGCCTCCTGTGCAGAACGCCGCCAGCTTGCATACCC
  CACCACCACAGCTTCCTGCGAGGCTGCCTCCGGCCAGTGTCCCAGCCACAGCCCTCCCTTCCACCTTACA
  GTTTTCACAGCAGTCACAGATGGTGGAGGCTTCAACTCAGCTCCAAATCCCTGTGAAGACTCAACAGCTA
  AATGCCCCCATCCCTGCCCCCCTGCCCAGCCAGCTCCCTGCTCCTTCTTCACAGCCCGCCCAGCCGGCCC
  TACATGTTCCGATGCCCGGGAAGGCGCAGATGCAGACATCTCAGCTTTCCTCCCAGACCCAGACAGTGGC
  ATCGACCAGGCCCCCCTTGGACTCTGCCCAGCCCTGCCAGCGGTCTCTGCCCACTTCCTCCTCTTCCTCA
  TCCCTTGTACCTGTGAGTGGCTCAGGTCCTGGACCCTCACCTGCTCGTTCTTCACCAGTGAACAGACCCT
  CTTCTGCTACCAACAAGGCTCTGTCTCCCATCACTTCTCGGTCTCCAGGGGTGGCAGTATCAGCTCCTCC
  TAAGCCACAGAGTCCTGCTCAGAATGCAGCCTCATCCCAGGACGGTTCTCAGGATAAACTGGCAGAACAA
  ATAACACTGGAAAACCAGATCCATCAGCGAATAGCAGATTTAAGGAAGGAAGGCTTGTGGTCCCTAAGGC
  GGTTGCCAAAGCTTCAAGAGGCCCCACGCCCGAAATCTCACTGGGACTATTTGCTGGAAGAGATGCAGTG
  GATGGCCACAGACTTTGCTCAAGAGCGGAGGTGGAAGTTGGCTGCTGCTAAGAAGCTTGTCAGAACTGTA
  GCCCGGCATCATGAGGAAAAGAAACTTCGTGAGGAAAGAGGGAAGAAGGAAGAACAGAGCAGACTGAGGC
  GCATTGCTGCCACTACTGCCAGAGAAATAGAATATTTTTGGTCAAATATTGAACAGGTTGTGGAAATAAA
  ATTACAAGTAGAATTAGAAGAGAAAAGAAAAAAAGCCTTAAATTTACAGAAAGTTTCCAGAAGAGGTAAA
  GAGTCCAGATTAAAGGGATTTGATACATCACCAGAACACTCTCTGGATCTAGGAATATCTGGAAGGAAAA
  GAAAAGCTAGCACATCTTTGACTGATGATGAAGTGGAAGATGAAGAAGAGACCATTGAAGAGGAGGAAGC
  ACACGAAGGGCTTGTAGACCACCACACAGAACTTACTAATCTGGCCAAAGAAGCTGAGTTGCCCTTAATC
  GATTTGATGAAGTTGTATGAAGGTGCCTTTCTGCCAAATTTTCAGTGGCCACAGCCTGAACCTGATCATG
  AAGAGTCAAGTGGGGAAGAAGATGTAGAAGACTGTCCTAGTGACAGGGAGAGCCGGAGGGACTCCGTTCT
  CATTGACTCACTCTTCATCATGGATCAGTTTAAAGCTGCAGAGAGAATGAGCATTGGAAAATCCAACACC
  AAGGACATCACAGAAGTTACTGCTGTGGCTGAAGCCATCCTCCCTAAGGGCAGTGCCCGAGTCACAACTG
  CGGTGAAGTTTAGTGCTCCATCTTTGTTGTATGGTGCTCTCCGAGACTATCAGAAGATAGGCCTGGACTG
  GTTGGCCAAGCTATACCGGAAGAATCTCAATGGCATATTGGCTGATGAAGCAGGGCTTGGGAAAACTGTG
  CAGATCATTGCTTTTTTTGCTCACCTTGCCTGTAATGAAGGTAACTGGGGCCCTCATCTTGTTGTCATGA
  GGAGTTGTAACATACTCAAGTGGGAACTCGAGTTGAAACGCTGGTGTCCTGGCCTCAAAACTCTTTCTTA
  TGTTGGCAGCCACAGAGAACTCAAAGCAAAGAGACAGGAGTGGACCGAGCCCAATAACTTCCACATCTGC
  ATCACGTCCTATAAGCAGTTCTTCAGGGGCTACACTGCCTTCTCAAGGGTGCACTGGAAATGCCTTGTTG
  TTGACGAAATGCAGAGGGTGAAGGGCATGACTGAGCGGCACTGGGAAGCCATCTTCAAGCTGCAGAGCCA
  GCAGCGCCTGCTTCTGATCGATGTTCCTCTGCACAACACTTTCCTGGAGCTCTGGACCATGGTGCACTTC
  CTCATTCCTGGGATCTCCAGGCCTTATCTGAGCTTTCCTCTGAAAGCCCCCAATGAGGAAAACCAGGATT
  ACTATCACAAAATGGTCATAAGGTTACACAGGGTCACACAGCCATTTATTTTGAGGAGAACCAAGAGAGA
  TGTGGAGAAACAGCTAACCAGGAAATATGAGCATGTTCTGAAGTGCCGCCTTTCCAGTCGTCAGAAGGCC
  TTATATGAGGATGTCATCCTACAGCCTAGGACTCAGGAAGCTCTGAAGAGTGGACATTTTGTCAGTGTCT
  TGAGTGTTCTGACACGGCTACAGCGAATCTGCAACCATCCTGGACTAGTAGAGCCACGGGTACCAGGCTC
  CTCCTTTGCAGCAGGCTCTCTGCAGTATAAGTCAGCCTCTCTAATTCTCAGAGTTCTGGAGAGAGAGTTC
  TGGAAGGAAACCGACCTTTCTATATTTGATCTCATTGGGCTAGAAAATAAAATCACTCGCCATGAAGCTG
  AATTACTGTGTAAGAAAAAAGTGACTCGGAAACTCATGGAGGAAGTCTTTGCTTCACCTCCTCCGTCAGC
  CCGACCAGCAGCAGTGAAGCTCAAGGCCAGCAGGTTATTTCAGCCTGTGCAATATGGCCAGAAGCCTGAG
  GGTCGCACTGTGGCATTCCCCAGCACACATCCACCTCGGATGGCAAACACCAACACATCCACCGCCACTC
  CACAGGGCCAGGTTCGAGGACGGCCGCCGATTGCTACTTTCTCTGCCAACCCAGATACAAAAGGTGGGGA
  AGTAGTGAAAATAGCTCAGCTGGCGTCTATTGCAGGGCCACAGAGCCGTGTGGCCCAGCCAGAGACACCT
  GTGACACTACAGTTCCAAGGGAACAAATTCACCTTGTCACATAGCCAGCTGCGGCAGCTCACAGCAGGCC
  AGCCTCTGCAGCTGCAAGGTAGTGTACTCCAGATCGTGTCAGCCCCTGGACAGCCCTACCTTCGAGCCCC
  TGGCCCTGTGGTGATGCAGACTGTGTCTCAGGCAGGTGCTGTGCATAGCACCCTGGGGAGCAAGCCCCCA
  ACCAGTGGCCCCAGCCCTGCACCCTTGACCCCACAAGTTGGTGTTCCGGGTCGAGTAGCAGTGAGTGCTA
  TGGCTGTTGGAGAACCTGGCCTGGCTTCCAAACCAGCTTCTCCTGCTGCAGGGCCAACCCAGGAGGAGAA
  GAGCCGACTTTTGAAGGAACGGTTGGATCAGATTCATTTTATCAATGAACGACGTTGTTCCCAGGCTCCA
  GTGTATGGCAGAGACTTGCTGAGGATTTGTTCCCTTCCTGGAAGAAGGAAGAGGCCTTTGTGCTGGTCTT
  TGGATAGCAACTTTGGGAAGGGTCCTAAAGGAGTAAACTATGACATGTCACTCTCAAAGAGTGAAGGTGA
  TCTGATTTTGACATTGAGTCAAGAGTCTCTTCAAGATGTTCTTGGCAGGGTGGCCTGTGTGATTCCTCCA
  GTGGTGGCTACACCACCCTCCCTGTGGGTCGCGAGGCCGCCCTCATTGTATAGCAGCAGGTTGAGAGCTC
  TGAGGCAGTGCCTGAGGGAGCACACCGGTCCTTACCACCGGCAGCTGCAACAACTAACAGCACTGCGCTC
  ACTGCAGTTCCCGGAGCTGAGGCTGGTGCAGTTTGACTCAGGGAAGTTAGAAGCTTTAGCAATCCTGCTA
  CAGAAACTAAAATCTGAAGGACGTCGGGTACTGATTTTATCTCAGATGGTTCTTATGCTAGACATTTTAG
  AGATGTTCTTGAACTTCCATTATCTCACTTATGTACGGATTGATGAAAATGCCAACAGTGAACAACGACA
  GGAGCTGATGAGGAGCTTCAACAGAGACAGGCGGATTTTCTGTGCCCTTCTCTCTACCCATAGTCGTGCC
  ACTGGCATAAACCTTGTTGAGGCTGACACTGTGGTGTTTTATGACAATGACCTAAATCCAGTTATGGATG
  CCAAAGCCCAGGAGTGGTGTGATAGAATTGGGAGGTGCAAGGACATCCACATATACAGGCTTGTCAGTGG
  CAATTCTATTGAAGAGAAATTGTTAAAAAATGGAACTAAAGATTTGATCCGTGAAGTAGCTGCTCAAGGA
  AATGACTATTCAATGGCCTTCTTAACGCAGCGAACTATCCAGGAGCTGTTTGAAGTCTACTCACCTATGG
  ATGATACTGGCTTTCCTGTGAAAGCTGAGGAATTCGTGGTGCTTTCTCAGGAACCATCTGTCAGTGAAAC
  TATCGCACCCAAGATTGCAAGACCTTTCATAGAGGCCCTCAAGAGCATCGAGTGTCTGGAGGAAGATGCA
  CAGAGGTCCACAGAGGAAGCTGTGCCTGGATCCAGCAGTGTAGCTGTCTCCTCAGACTCAGATGGCTCTA
  GATATGACGAGGAACCATCACAGCTGGAGGAGCTGGCTGACTTTATGGAACAGCTTACTCCAATTGAAAA
  ATATGCATTGAATTACTTGGAATTGTTCCATACGACCACTGAACAAGAAAAAGAGAGAATCAGTGAGGAT
  TTGGTAATGGCGTCAATGAAGGACTGGGAAACCCGAAATGCTAGAGCCCTGCAGGAAAGGGAGGCCCGGC
  TACAGCTGGAGCAGGAGGAGGCAGAGCTCCTCACCTACACTCGGGAGGACGCCTATACTATGGAGTATGT
  CTATGAAGATGCTGATGGACAAACAGAAGTCATGCCGCTCTGGACACCACCCACCCCTCCTCAGGATGAC
  AATGATATCTACATTGACTCAGTTATGTGTCTCATGTATGAGACCACACCCATCCCAGAAGCTAAGCTGC
  CTCCTGTTTATGTGAGGAAGGAGCGCAAGCGGCACAAAACAGACCCCTCAGCTGCAGGCCGGAAGAAGAA
  GCAGCGGCATGGTGAGGCAGTTGTCCCGCCCCGGTCCTTGTTTGACCGGGCAACACCTGGTATGTTGAAA
  ATCCGGCGTGAAGGCAAAGAGCAGAAGAAGAACCTGCTGCTGAAGCAGCAGACACCTTTTGCCAAGCCTC
  TGCCCACCTATGTCAAGTCCTCAGGGGAACCTGCCCAGGACAGTCCTGACTGGCTCATTGGCGAGGACTG
  GGCCTTGCTACAGGCTGTAAAGCAGTTGCTTGAGCTGCCCTTGAACCTCACGATTGTGTCACCTGCCCAC
  ACTCCTAATTGGGACCTTGTCAGTGATGTTGTCAACTCCTGTAGTCGAATCTACCGTTCTTCCAAGCAGT
  GCCGGAATCGTTATGAGAACGTCATCATTCCCAGGGAGGAGGGGAAGAGTAAGAACAATCGTCCTCTCCG
  CACAAGCCAGATTTATGCTCAGGATGAAAACGCCACTCACACCCAGTTGTACACGAGCCACTTTGAGCTG
  ATGAAAATGACAGCTGGCAAGAGAAGCCCCCCAATCAAGCCTCTGCTTGGCATGAATCCCTTTCAGAAGA
  ACCCCAAGCATGCCTCTGTCCTGGCAGAAAGTGGAATCAACTATGACAAGCCGCTGCCTCCCATTCAAGT
  TGCATCTCTCCGTGCAGAGCGAATTGCAAAAGAGAAAAAGGCTTTGGCTGATCAGCAGAAGGCACAGCAG
  CCACCAGTGACACAGCCGCCCCCTCAGCAACAACAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCAGC
  AGCAGCAACCCCCGCCACCTCCACAGCAGCCACCACCACCAGTGCCGCAGCCACAGGCAGCAAGCAGTCA
  GACACCAGCAGGGCAGCCTGCTGTCCAGCCCCAGCCCCAGCCCCAGGTCCAGACCCAGCCACAGCCTGTG
  CAGCCACAGTCAAAGGGGCAGCCCACAATGACGACTGTGGGCAGTGCTGCTGTGCTGGCAGGAACCATTA
  AAACATCAGTCACTGGAACAAGCATCCCTACAGGGACTGTAAGTGGAAATGTGATTGTGAACACAATAGC
  TGGTGTCCCTGCTGCTACTTTCCAGTCCATTAACAAGCGTCTGGCATCACCTGTGGCTCCTGGAACTTTG
  ACTACGTCTGGAGGTTCTGCTCCTGCTCAGGTGGTCCACACTCAACAACGAGCAGTTGGTTCCCCAGCCA
  CTGCCACCACTGACCTGGTATCCATGACAACTACTCAAGGTGTTCGAGCAGTTACTTCAGTGACGGCCTC
  AGCTGTGGTCACTACCAACCTGACTCCAGTGCAGACTCCAACCCGTTCCTTAGTAACCCAGGTGTCTCAA
  GCCACAGGGGTTCAGCTACCTGGAAAAACCATCACCCCTGCTGCTCATTTCCAGCTTCTTAGGCAGCAGC
  AGCAACAGCAGCAGCAGCAGCAGCAGCAACAGCAGACTTCCCAGGTGCAAGTTCCACAGCTCCAGAGCCA
  GGCCCAATCTCCTGCACAGATCAAAGCTGTGAGCAAGCTAGGACCGGAACACATCATCAAGATGCAGAAA
  CAGAAGATGCAGCTGCCACCACAGCCCCCACCACCGCAGGCCCAGCCAGGTCCCCCACAGCAACCAGCGC
  AAGTGCAAGTACAGACTCCACAGCCCCCACAGCAACAGCAGAGTCCTCAGCTTACCACAGTCACGGCCCC
  ACGGCCTGGGGCTTTGCTCACAGGCACCACAGTGACCAACCTTCAGGTGGCCCGGCTTACCCGAGTCCCT
  ACTTCTCAGCTGCAGGCACAAGGGCAGATGCAAACTCAGACGCCCCAGCCTGCTCAGGTGGCCTTGGCAA
  AGCCTCCGGTGGTATCTGTCCCAGCAGCTGTGGTCTCCTCGCCAGGGGTCACGACCCTGCCTATGAACGT
  TGCAGGGATCAGCGTGGCAATTGGACAGCCACAAAAAACAGCAGGGCAGACGGTCGTGGCCCAACCCGTC
  AATGTCCAACAGCTGCTGAAGTATAAGCAGCAGACAGCCGTCCAACAGCAGAAGGCCATCCAGCCACAAG
  TTGCCCAGGGCCAGGCTGCTGTTCAGCAGAAGCTGACCACCCAACAGATCACTACTCAGGGCCCGCAGCA
  GAAGGTCGCTTATGCTGCCCAGCCAGCCCTTAAAACCCAGTTTCTGACCACACCCATCTCCCAGGCCCAG
  AAACTGGCCGGGACTCAGCAGGTTCAGACTCAGATCCAGGTTGCAAAACTTCCTCAAGTTGTGCAGCAAC
  AAACACCTGTGGCCAGTATACAACAAGTTGCATCAGCTTCTCAGCAGGCTTCTCCACAGACGGTGACGCT
  CACACAGGCAACGGCAGCAGGGCAACAGGTACAGATGATCCCAACGGTGACTGCAACAGCCCAGCTGGTG
  CAGCAGAAGCTCATCCAGCAGCAGGTGGTGACGACAGCCTCAGCATCACTGCAGACCCCTGGTGGCCCAA
  GCCCAGCACAGCTTCCAGCCAGCTCTGACAGCCCAAGCCAGCAGCCAAAGTTGCAAATGAGAGTTCCTGC
  TGTGAGGCTGAAGACGCCCACCAAACCTCCATGCCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173066
• Insert Size: 9000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173066.1, NP_775089.1
• RefSeq Size: 10690 bp
• RefSeq ORF: 9000 bp
• Locus ID: 75560
• UniProt ID: Q8CHI8
• Cytogenetics: 5 F
• Gene Summary: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome (By similarity). Regulates transcriptional activity of ZNF42.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2), also called mDomino-S, lacks an in-frame exon, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.