Ubr5 (NM_001112721) Mouse Untagged Clone

CAT#: MC225409

Ubr5 (untagged) - Mouse ubiquitin protein ligase E3 component n-recognin 5 (Ubr5), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubr5
• Synonyms: Edd; Edd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225409 representing NM_001112721
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTCCATCCATTTCGTGGTCCACCCGCTGCCGGGCACCGAGGACCAGCTCAATGACAGGTTACGGG
  AAGTTTCAGAGAAGCTGAACAAGTACAATTTGAACAGCCACCCACCTCTGAATGTACTGGAGCAGGCTAC
  TATTAAACAGTGTGTGGTGGGACCCAATCACGCCGCGTTTCTTCTTGAGGATGGCAGAATTTGTAGGATT
  GGTTTTTCAGTACAACCAGACAGACTGGAATTGGGTAAACCTGATAATAATGATGGGTCAAAACTGAACA
  GCAGCTCGGGGACAGGGAGGACCTCTAGGCCAGGAAGGACAAGTGACTCCCCGTGGTTTCTCTCAGGTTC
  TGAGACTCTGGGCAGGTTGGCAGGCAACACTCTGGGAAGCCGCTGGAGTTCTGGCGTGGGGGGAAGTGGA
  GGAGGCTCCTCTGGTCGGTCGTCTGCTGGAGCTCGTGATTCCCGGCGACAGACTCGAGTCATTCGGACTG
  GCCGGGATCGGGGGTCTGGACTCCTGGGCAGTCAGCCCCAGCCAGTTATTCCAGCATCTGTCATTCCTGA
  GGAGCTGATATCACAGGCACAAGTTGTTCTACAAGGCAAATCGAGAAGTGTCATTATTCGAGAACTTCAG
  AGAACAAATCTTGATGTCAACCTTGCTGTAAATAACTTACTGAGCCGGGATGATGAGGATGGAGATGACG
  GTGATGACACAGCCAGTGAATCTTACCTGCCGGGAGAAGATCTTATGTCTCTTCTTGACGCTGACATTCA
  CTCTGCCCACCCAAGTGTCATTATTGATGCTGATGCCATGTTTTCTGAGGACATTAGCTATTTTGGTTAT
  CCTTCTTTTCGTCGTTCATCACTCTCTAGGCTAGGCTCATCACGAGAGAGAGACTCAGAGCTTCTGCGGG
  AACGGGAGTCTGTTCTACGTTTACGGGAGCGGCGGTGGCTTGATGGAGCCTCATTTGATAATGAAAGGGG
  CTCTACCAGCAAAGAGGGAGAGTCAAACCCGGATAAGAAGAACACGCCTGTTCAGAGTCCAGTGTCTCTG
  GGAGAAGACTTACAGTGGTGGCCTGATAAGGATGGAACAAAATTTACCTGCATTGGGGCTCTGTATTCTG
  AGCTTCTTGCTGTCAGCAGCAAAGGAGAACTTTATCAATGGAAATGGAGTGAATCTGAGCCTTACAGAAA
  TGCCCAGAATCCTTCATTGCATCATCCACGAGCAACATTTTTGGGGTTGACCAATGAAAAGATAGTCCTC
  TTGTCTGCAAATAGCATAAGAGCAACTGTAGCTACTGAAAATAACAAGGTTGCTACATGGGTGGATGAAA
  CATTAAGTTCTGTGGCTTCTAAGTTAGAACACACAGCACAGACTTACTCTGAACTTCAAGGAGAACGGAT
  AGTTTCTTTACATTGCTGTGCCCTTTATACCTGTGCCCAGCTGGAGAATAATTTGTATTGGTGGGGTGTT
  GTTCCTTTCAGTCAAAGGAAGAAAATGTTAGAGAAAGCTAGAGCAAAAAATAAAAAGCCCAAATCCAGTG
  CTGGTATTTCTTCCATGCCAAACATCACTGTTGGAACTCAGGTGTGCTTGAGAAATAACCCTCTCTACCA
  TGCTGGAGCGGTTGCCTTTTCAATTAGTGCTGGCATTCCTAAAGTTGGTGTCTTGATGGAGTCAGTATGG
  AATATGAATGATAGCTGTAGATTTCAACTTCGGTCTCCAGAGAGCCTGAAGAGCATGGAAAAAGCCAGCA
  AAACTCTTGAGACAAAGCCCGAGAGTAAGCAGGAACCAGTAAAAACAGAAATGGGCCCTCCACCATCACC
  AGCATCCACCTGCAGCGACGCGTCCTCAATTGCCAGCAGTGCATCCATGCCATACAAACGACGTCGGTCA
  ACTCCTGCCCCGAGAGAAGAGGAGAAGGTGAACGAGGAGCAGTGGCCCCTTCGGGAAGTTGTTTTTGTTG
  AAGATGTTAAGAACGTTCCTGTTGGCAAGGTGCTGAAGGTAGATGGTGCCTATGTCGCTGTAAAGTTTCC
  AGGAACATCTACTAACACCACCTGTCAGAACAGCTCTGGTCCAGACGCCGACCCTTCCTCGCTCCTGCAG
  GACTGCAGGCTGCTTCGAATTGATGAGCTGCAGGTTGTCAAAACGGGCGGCACCCCAAAAGTTCCCGACT
  GTTTTCAGAGGACTCCCAAAAAGCTTTGTATACCCGAGAAGACTGAAATACTAGCAGTAAATGTGGATTC
  CAAAGGTGTCCATGCTGTTCTGAAGACTGGAAGCTGGGTTCGGTACTGTGTCTTTGATCTTGCCACAGGG
  AAGGCTGAACAAGAGAATAATTTCCCAACAAGCAGTGTTGCTTTTCTTGGTCAGGATGAGAGGAGTGTCG
  CTATCTTCACTGCTGGGCAGGAATCTCCTATTGTTCTTCGAGATGGAAATGGTACTATCTACCCAATGGC
  CAAGGATTGCATGGGAGGAATACGGGATCCTGACTGGCTTGATCTCCCACCAATCAGTAGTCTTGGGATG
  GGTGTACATTCTTTAATAAATCTTCCTGCCAACTCAACAATCAAAAAGAAAGCAGCTATTATCATCATGG
  CTGTAGAGAAGCAAACCTTAATGCAGCACATTCTCAGATGTGACTACGAGGCCTGCCGACAGTATCTCGT
  GAATCTGGAGCAAGCCGTGGTTTTAGAGCAGAACCTGCAGATGTTACAGACGTTCATCAGCCACAGATGT
  GATGGGAACCGGAACATTTTACATGCTTGTGTATCAGTTTGCTTTCCAACCAGCAATAAGGAAACTAAAG
  AAGAAGAGGAAGCTGAGCGCTCAGAAAGAAATACATTTGCTGAAAGGCTTTCTGCTGTGGAGGCCATTGC
  AAATGCAATATCAGTTGTTTCAAGTAACGGCCCAGGTAATCGGGCTGGATCATCAAATAGTCGAAGTTTG
  AGATTACGAGAAATGATGAGACGTTCATTGAGAGCAGCTGGTTTGGGTAGACATGAGGCTGGAGCTTCAT
  CCAGTGACCACCAGGATCCAGTTTCACCCCCCATAGCTCCCCCTAGCTGGGTTCCTGACCCTCCTTCAAT
  GGATCCTGATGGTGATATTGATTTTATCCTGGCCCCCGCTGTGGGATCTCTTACCACAGCAGCGACTGGC
  AGTGGTCAAGGACCCAGCACCTCCACCATTCCAGGTCCTTCCACAGAGCCATCTGTAGTAGAATCCAAGG
  ATCGAAAGGCAAACGCACATTTCATATTAAAATTACTATGCGACAGTGCGGTTCTGCAGCCTTATCTACG
  AGAGCTTCTTTCTGCCAAGGATGCAAGAGGAATGACCCCGTTTATGTCAGCTGTAAGTGGCCGGGCTTAT
  CCTGCTGCAATCACCATCCTAGAAACAGCCCAGAAGATTGCCAAAGCTGAAGTATCTGCAAGTGAAAAGG
  AGGAAGATGTGTTCATGGGAATGGTTTGCCCGTCAGGTACCAACCCTGACGACTCCCCTTTGTATGTCTT
  ATGTTGCAACGACACCTGCAGCTTTACCTGGACTGGAGCAGAGCACATTAACCAGGACATTTTTGAATGT
  CGGACTTGTGGTTTACTGGAGTCGCTTTGTTGTTGTACAGAATGTGCAAGGGTTTGTCATAAAGGGCACG
  ATTGCAAACTCAAGAGGACTTCACCAACAGCTTATTGTGATTGTTGGGAGAAATGTAAATGTAAAACATT
  GATTGCTGGACAGAAGTCTGCTCGTCTTGATCTACTTTATCGCCTGCTTACTGCTACAAATCTGGTCACC
  CTACCAAACAGTAGGGGAGAGCACCTTTTGCTGTTCTTAGTACAGACCGTGGCCAGGCAGACAGTGGAGC
  ACTGTCAGTATAGGCCTCCACGAATCAGGGAGGATCGCAACAGGAAAACAGCCAGTCCTGAAGATTCAGA
  TATGCCTGACCATGACTTAGAGCCCCCAAGGTTTGCCCAGCTTGCCCTAGAGCGCGTCCTTCAGGACTGG
  AATGCTTTGAGATCTATGATTATGTTTGGGTCACAAGAGAACAAAGATCCTCTGAGTGCCAGCAGCAGGA
  TAGGCCATCTTTTGCCAGAAGAGCAGGTCTACCTCAACCAGCAGAGCGGCACGATTCGGCTCGACTGTTT
  CACTCATTGCCTTATTGTCAAGTGTACAGCAGATATTTTGCTTTTAGATACTCTGCTAGGCACCTTAGTA
  AAAGAACTCCAAAACAAGTACACACCCGGACGCCGGGAAGAAGCAATTGCCGTGACAATGAGGTTTCTAC
  GATCTGTGGCAAGAGTGTTTGTCATTCTTAGTGTGGAAATGGCTTCGTCCAAAAAGAAAAACAACTTTAT
  TCCACAGCCAATTGGAAAATGCAAACGTGTGTTTCAAGCACTGCTTCCTTATGCTGTAGAAGAATTGTGT
  AATGTGGCAGAGTCTCTGATTGTTCCTGTCAGAATGGGGATTGCCCGGCCGACAGCACCGTTTACTCTGG
  CCAGTACTAGCATAGATGCCATGCAGGGCAGTGAAGAATTATTTTCAGTAGAACCATTACCACCACGGCC
  GTCATCAGATCAGGCAAGCAGCTCCAGTCAGTCTCAGTCATCTTACATCATTCGGAACCCACAGCAGAGG
  CGCATCAGCCAGTCACAGCCCGTCAGAGGCCGAGACGAGGAGCAGGATGATATTGTGTCAGCAGACGTGG
  AAGAGGTTGAGGTGGTGGAAGGCGTGGCTGGAGAGGAGGATCATCATGATGAACAGGAAGAGCACGGGGA
  AGAGAATGCTGAGGCTGAGGGACATCACGATGAGCATGATGAAGACGGAAGTGACATGGAGCTAGATTTG
  TTAGCAGCAGCTGAAACGGAAAGTGACAGTGAAAGTAACCATAGTAACCAAGATAATGCTAGTGGGCGCA
  GAAGTGTTGTCACCGCAGCCACTGCTGGCTCAGAAGCAGGAGCGAGCAGTGTTCCTGCCTTTTTTTCTGA
  AGATGACTCTCAGTCAAATGACTCAAGTGACTCTGACAGCAGTAGCAGTCAGAGTGATGACATAGAGCAG
  GAGACCTTTATGCTCGATGAGCCACTAGAAAGGACAACGAATAGCTCCCATGCAAATGGTGCTGCCCAGG
  CTCCTCGCTCCATGCAGTGGGCTGTCCGAAATCCGCAGCATCAGCGAGCAGCAAGCACAGCTCCGTCCAG
  CACATCTACGCCAGCAGCAAGTTCTGCAGGTTTGATTTATATTGATCCTTCAAATTTACGCCGGAGCGGC
  ACCATCAGTACAAGTGCTGCAGCGGCAGCAGCTGCTTTGGAAGCTAGCAATGCCAGCAGCTACTTAACAT
  CTGCAAGCAGTTTAGCCAGGGCTTACAGCATTGTCATCAGACAAATCTCGGACTTGATGGGCCTTATCCC
  TAAGTATAATCACCTAGTATACTCTCAGATTCCAGCAGCTGTGAAATTGACTTACCAAGATGCAGTCAAC
  TTACAGAACTATGTAGAAGAAAAGCTTATTCCTACGTGGAACTGGATGGTCAGTGTTATGGATTCTACTG
  AAGCTCAATTACGTTATGGTTCTGCACTAGCATCTGCTGGTGACCCAGGACACCCAAATCATCCCCTTCA
  TGCCTCTCAGAATTCAGCTCGAAGAGAGAGGATGACTGCACGGGAAGAGGCCAGTCTAAGAACGCTTGAA
  GGCAGACGACGTGCTACATTGCTGAGCGCCCGCCAGGGAATGATGTCTGCTCGAGGAGACTTTCTCAATT
  ATGCTCTGTCTTTAATGAGATCTCATAATGATGAGCATTCTGATGTTCTTCCAGTATTGGATGTGTGCTC
  ATTGAAACATGTGGCATATGTTTTCCAAGCCCTTATATACTGGATTAAAGCAATGAATCAGCAGACAACA
  TTAGACACTCCTCAACTGGAACGCAAAAGGACACGAGAACTTCTGGAACTGGGTATTGATAATGAAGATT
  CAGAACATGAAAATGATGATGACACCAGTCAAAGTGCTACTTTGAATGATAAGGATGACGACTCTCTTCC
  TGCAGAAACTGGCCAAAACCATCCATTTTTCCGACGTTCAGACTCTATGACATTCCTTGGATGTATACCT
  CCAAATCCATTTGAAGTGCCTTTGGCTGAAGCCATACCCTTGGCTGATCAGCCACATTTGTTGCAGCCAA
  ATGCTAGAAAGGAAGATCTTTTTGGTCGCCCAAGTCAGGGTCTTTATTCCTCATCTGCCGGAAGTGGGAA
  ATGCATAGTAGAGGTTACAATGGACAGAAATTGTCTCGAGGTTCTTCCAACTAAAATGTCTTATGCTGCC
  AATCTAAAAAATGTAATGAACATGCAAAACCGGCAGAAGAAAGAAGGGGAAGAACAGAGCCTGCTGGCCG
  AAGAAGCTGACAGTTCAAAGCCAGGTCCATCCGCTCCTGATGTTGCTGCCCAGTTGAAAAGCAGTTTACT
  AGCAGAGATAGGACTCACGGAAAGTGAAGGGCCCCCCCTTACATCTTTCAGGCCTCAGTGCAGTTTCATG
  GGCATGGTTATCTCCCATGACATGCTGCTGGGACGCTGGCGCCTGTCTTTAGAACTGTTCGGCAGAGTAT
  TCATGGAAGATGTTGGTGCAGAGCCTGGATCGATTTTAACTGAATTGGGTGGTTTTGAGGTAAAAGAATC
  AAAGTTCCGTAGAGAGATGGAAAAACTGAGAAACCAGCAATCAAGAGATTTATCACTAGAGGTCGATCGC
  GATCGGGATCTTCTCATTCAGCAGACTATGAGACAGCTTAACAACCATTTTGGCCGAAGATGTGCTACCA
  CGCCAATGGCTGTGCACAGAGTCAAAGTCACCTTTAAGGATGAACCAGGAGAGGGCAGTGGTGTAGCAAG
  AAGTTTCTATACAGCCATTGCACAAGCGTTTTTATCAAATGAAAAATTGCCAAATCTAGATTGTATCCAA
  AATGCCAACAAAGGTACTCATACAAGTTTAATGCAAAGATTGAGGAACAGAGGTGAAAGAGACCGGGAGA
  GAGAGAGAGAACGGGAAATGAGGAGGAGCAGTGGCTTGCGTGCAGGATCTCGAAGAGACAGGGATAGAGA
  CTTCAGGAGACAGCTCTCCATTGACACCAGGCCTTTCAGACCTGCTTCTGAGGGGAATCCTAGTGATGAC
  CCTGACCCTCTTCCAGCTCATCGGCAGGCACTTGGAGAGAGGCTTTACCCCCGAGTGCAAGCAATGCAGC
  CCGCATTTGCAAGTAAAATCACTGGCATGCTGTTGGAGTTATCCCCAGCTCAGCTGCTTCTCCTTCTAGC
  AAGTGAAGATTCTCTGAGGGCGAGAGTCGACGAGGCCATGGAACTCATTATTGCTCATGGACGGGAAAAT
  GGAGCTGACAGTATTTTGGACCTCGGATTATTAGACTCTTCAGAAAAGGTACAGGAAAACAGAAAGCGCC
  ATGGCTCTAGTAGAAGTGTAGTGGATATGGATTTAGAGGACACAGATGATGGTGATGACAACGCTCCCTT
  GTTTTACCAACCTGGGAAGAGAGGATTTTATACTCCGAGACCTGGCAAAAACACAGAAGCAAGGCTGAAT
  TGTTTCAGAAACATCGGCAGGATTCTTGGACTATGTCTGTTGCAAAATGAACTATGTCCTATCACATTGA
  ATAGGCATGTAATTAAAGTGTTGCTTGGTAGAAAAGTCAACTGGCATGACTTTGCTTTTTTCGACCCTGT
  AATGTATGAGAGTTTACGACAACTGATCCTTGCATCTCAGAGTTCAGATGCTGATGCTGTTTTCTCAGCA
  ATGGATTTGGCATTTGCAATTGACCTGTGTAAAGAAGAGGGTGGAGGACAGGTTGAACTTATTCCAAATG
  GTGTAAATATACCAGTCACTCCGCAGAATGTATATGAGTATGTAAGGAAATATGCAGAGCATAGAATGTT
  GGTGGTTGCAGAACAGCCATTGCACGCAATGAGAAAGGGTCTACTAGACGTGCTTCCAAAAAATTCCTTA
  GAAGACTTAACAGCTGAGGACTTCCGGCTCTTGGTGAACGGCTGTGGTGAAGTCAATGTGCAGATGCTGA
  TCAGTTTCACTTCTTTCAATGATGAGTCAGGAGAAAATGCTGAGAAGCTCCTCCAGTTCAAACGGTGGTT
  CTGGTCAATAGTAGAGAAAATGAGCATGACAGAACGGCAGGACCTGGTTTACTTCTGGACATCTAGCCCA
  TCATTGCCAGCCAGTGAAGAAGGTTTTCAGCCTATGCCCTCGATCACAATAAGACCACCAGATGACCAAC
  ATCTTCCTACTGCAAATACTTGTATTTCTCGACTTTATGTCCCACTCTATTCCTCTAAACAGATTCTCAA
  ACAAAAATTACTACTCGCCATTAAGACCAAGAATTTTGGTTTTGTGTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001112721
• Insert Size: 8379 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001112721.2, NP_001106192.1
• RefSeq Size: 9773 bp
• RefSeq ORF: 8379 bp
• Locus ID: 70790
• UniProt ID: Q80TP3
• Cytogenetics: 15 B3.1
• Gene Summary: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (By similarity). Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Plays an essential role in extraembryonic development. Regulates DNA topoisomerase II binding protein (TopBP1) for the DNA damage response.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.