Speg (NM_001085370) Mouse Untagged Clone

CAT#: MC225343

Speg (untagged) - Mouse SPEG complex locus (Speg), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Speg
• Synonyms: Apeg1; AW125581; BPEG; D1Bwg1450e; mKIAA1297; SPEGalpha; SPEGbeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225343 representing NM_001085370
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTGGCGCCAGGAGCTGACGTGCTACTTAAGTGTATCATCACCGCCAACCCCCCACCCCAAGTGT
  CCTGGAAAAAGGATGGGTCCATGTTGCACAGCGAGGGTCGTCTTCTCATCCGGGCTGAAGGTGAACGGCA
  CACACTGCTGCTCAGAGAGGCCCAGGCTGCCGATGCTGGGAGCTACACAGCCACTGCCACCAACGAACTG
  GGCCAAGCTACCTGTGCTTCTTCACTGGCTGTGAGACCTGGCGGCTCCACATCCCCTTTCAGCAGCCCCA
  TCACCTCTGATGAGGAGTACCTGAGCCCCCCAGAGGAGTTCCCAGAGCCTGGGGAGACCTGGCCCCGAAC
  CCCTACCATGAAGCTCAGTCCCAGCCAGGATCATGATTCCTCCGACTCTTCTTCCAAGGCACCCCCAACG
  TTCAAGGTCTCACTCATGGACCAATCGGTGAGAGAAGGTCAAGATGTCATTATGAGCATCCGTGTGCAGG
  GAGAGCCCAAGCCTGTGGTTTCCTGGCTGAGGAATCGACAGCCCGTGCGCCCAGACCAGCGGCGCTTTGC
  AGAGGAGGCCGAGGGTGGGCTCTGCCGCTTGAGGATCCTGGCTGCTGAACGGGGCGATGCTGGTTTCTAC
  ACATGCAAGGCGGTCAACGAATATGGCGCTCGGCAGTGCGAGGCCCGCCTGGAGGTCCGAGCACACCCTG
  AAAGCCGGTCCCTGGCCGTGCTGGCCCCCCTGCAGGACGTGGACGTGGGGGCCGGGGAGATGGCGCTGTT
  TGAGTGCCTGGTGGCAGGTCCAGCTGACGTCGAGGTGGACTGGCTGTGCCGGGGCCGCCTGCTGCAGCCT
  GCGCTGCTCAAATGCAAGATGCATTTTGATGGCCGCAAGTGCAAGCTGCTGCTCACCTCTGTGCACGAGG
  ACGACAGTGGTGTCTACACCTGCAAGCTGAGCACAGCCAAAGATGAACTGACCTGCAGTGCCCGGCTGAC
  GGTTCGGCCGTCGCTGGCGCCTCTCTTCACCCGGTTGCTGGAAGATGTGGAGGTGTTGGAAGGCCGTGCT
  GCCCGCTTGGATTGCAAGATCAGCGGTACCCCGCCTCCCTCCGTTACCTGGACTCATTTTGGCCACCCCG
  TGAATGAGGGTGACAACTTGCGGCTTCAACAGGATGGAGGTCTACACTCACTGCACATTGCCCGGGTGGG
  CAGTGAGGACGAAGGACTCTATGAAGTCAGTGCCACCAACACTCATGGCCAGGCCCACTGCTCCGCCCAG
  CTGTATGTGGAGGAGCCTCGGACAGCTGCCTCTGGCCCCAGCTCGAAGCTGGAGAAGATGCCATCTATCC
  CTGAGGAGCCGGAGCACGGAGATCTAGAGAGGCTGTCTATTCCTGACTTCCTACGGCCACTGCAAGATCT
  GGAAGTGGGACTAGCTAAGGAGGCCATGCTGGAGTGCCAAGTGACCGGCCTGCCCTATCCCACCATCAGC
  TGGTTCCACAATGGCCACCGCATTCAAAGCAGTGACGACCGACGCATGACACAGTACAGGGACATACATC
  GCCTGGTGTTCCCAGCTGTGGGGCCTCAGCATGCTGGTGTCTATAAGAGTGTAATCGCCAACAAGCTGGG
  CAAAGCGGCCTGCTACGCCCATCTCTATGTCACAGATGTGGTTCCAGGCCCTCCAGATGGTGCTCCGGAA
  GTAGTAGCTGTGACCGGAAGGATGGTCACACTATCGTGGAACCCACCCAGGAGTCTGGACATGGCTATTG
  ACCCAGATTCCCTAACATACACTGTGCAGCACCAGGTGTTGGGCTCGGACCAGTGGACGGCACTGGTCAC
  AGGCCTGCGGGAGCCTGCGTGGGCAGCCACAGGGCTGAAGAAGGGGATCCAGCACATCTTCCGGGTCCTC
  AGCTCCAGTGGCAAGAGCAGCAGCAAGCCCTCAGCACCGTCGGAACCCGTGCAGCTGCTAGAGCATGGCC
  CACCCCTGGAAGAGGCGCCTGCTGTGCTGGACAAGCAGGACATTGTGTATGTGGTAGAGGGCCAGCCTGC
  CTGTGTCACCGTCACTTTTAACCATGTGGAGGCCCAGGTTGTCTGGAGGAGCTGCCGGGGAGCCCTCCTC
  GAGGCGCGCACAGGTGTGTATGAACTGAGTCAGCCAGATGACGACCAGTATTGTCTGCGGATCTGCCGGG
  TGAGCCGACGAGACCTGGGACCCCTTACTTGCAGTGCCCGAAACCGCCACGGCACAAAGGCCTGCTCGGT
  CACCCTGGAGCTGGCAGAGGCACCTCGGTTTGAGTCTATCATGGAAGATGTGGAGGTGGGGCCTGGAGAA
  ACCGCTCGCTTTGCTGTGGTAGTTGAGGGGAAACCACTGCCAGACATCATGTGGTACAAGGATGAGGTGC
  TGCTGGCCGAGAGCAACCATGTGAGCTTCGTGTATGAGGAGAACGAGTGTTCCCTAGTGCTGCTCAGCGC
  AGGAAGCCAGGATGGAGGCGTCTATACCTGTACCGCCCGGAACCTGGCAGGCGAAGTCTCCTGCAAGGCG
  GAGCTGTCTGTGCTTTCAGCTCAGACAGCCATGGAGGTCGAGGGGGTCGGAGAGGATGAGGAACACCGAG
  GAAGAAGACTTAGCGACTACTACGACATCCACCAAGAGATTGGCAGGGGTGCCTTCTCCTACCTGCGGCG
  TGTAGTGGAGAGGAGCTCTGGCCTGGAGTTTGCAGCCAAGTTCATCCCTAGCCAGGCTAAGCCTAAAGCA
  TCAGCAAGACGCGAGGCGCGCTTGCTGGCCCGGCTCCAGCATGGCTGTGTGCTCTACTTCCACGAAGCCT
  TTGAGAGGCGCAGGGGACTTGTCATAGTCACGGAGCTCTGCACAGAAGAGCTTCTAGAGCGCATGGCCAG
  GAAGCCCACCGTGTGTGAGTCTGAGACTCGGACCTATATGCGTCAAGTGCTAGAGGGGATATGCTATCTG
  CATCAGAGCCATGTGCTGCACCTGGATGTCAAGCCGGAGAACCTGCTTGTGTGGGATGGTGCAGGGGGTG
  AAGAGCAGGTGCGCATCTGTGACTTCGGGAACGCCCAGGAACTCACTCCGGGAGAGCCCCAGTACTGTCA
  GTATGGCACGCCAGAGTTTGTAGCACCTGAGATTGTCAACCAGAGCCCTGTGTCTGGAGTCACTGACATC
  TGGCCCGTGGGCGTCGTTGCCTTTCTCTGTTTGACGGGCATCTCCCCATTTGTTGGGGAGAATGACCGGA
  CAACACTCATGAATATCCGAAACTACAACGTGGCATTTGAGGAGACTACGTTCCTGAGTCTGAGCAGGGA
  GGCCCGGGGCTTCCTCATCAAAGTGCTGGTGCAGGATCGACTGAGACCTACTGCAGAAGAGACATTGGAA
  CATCCTTGGTTCAAAACAGAAGCAAAGGGTGCAGAGGTGAGCACAGATCACCTGAAGCTCTTCCTGTCAC
  GGAGGAGGTGGCAGCGCTCTCAAATCAGCTACAAGTGCCACCTGGTGCTGCGCCCCATTCCGGAACTGCT
  ACGGGCTCCCCCGGAGCGGGTGTGGGTGGCCATGCCTAGAAGACAACCCCCGAGTGGGGGACTCTCATCC
  TCTTCAGACTCAGAAGAGGAAGAGTTGGAGGAACTTCCCTCAGTACCCCGCCCCCTGCAGCCTGAGTTCT
  CAGGTTCTCGGGTATCCCTCACCGACATCCCCACCGAAGATGAGGCTCTGGGGACCCCGGAAGCTGGGGC
  TGCCACCCCCATGGACTGGCAAGAGCAGGAAAGGACTCCCTCTAAGGACCAGGAGGCCCCCAGCCCTGAG
  GCACTCCCCTCTCCAGGCCAGGAGTCCCCTGATGGGCCTAGCCCCAGGCGGCCAGAGCTCCGCAGGGGTA
  GCTCTGCTGAGAGTGCCCTGCCCCGAGTCGGGTCGCGGGAACCGGGCCGGAGCTTGCACAAGGCAGCATC
  CGTGGAGCTGCCGCAGCGCAGGAGCCCCAGCCCGGGTGCCACCCGCCTGACTCGGGGAGGCCTGGGTGAG
  GGCGAGTATGCCCAGAGGCTGCAGGCCCTGCGTCAGCGGCTGCTGAGGGGAGGCCCCGAGGATGGCAAGG
  TCAGCGGCCTCAGGGGACCCCTGCTGGAGAGCCTGGGGGGCCGTGCACGGGATCCCAGGATGGCAAGGGC
  TGCCTCCAGCGAGGCTGCCCCCCACCACCAGCCCCCTCCGGAGTCCCGGGGTCTGCAAAAGAGCAGCAGC
  TTCTCTCAGGGCGAGGCAGAGCCCCGGGGTCGGCACCGCCGCGCGGGGGCACCCCTCGAAATCCCCGTGG
  CCAGACTTGGGGCTCGCAGGCTGCAGGAGTCTCCCTCCTTATCTGCCCTTAGCGAGACCCAGCCACCGAG
  CCCTGCGAGGCCCAGCGTCCCCAAACTCAGTATCACCAAGTCCCCGGAACCTTCCGCTGTCACATCCAGA
  GACTCTCCCCAGCCCCCGGAACCTCAGCCTGTCCCAGAAAAGGTCCCTGAGCCCAAACCAGAACCAGTCC
  GAGCTGCAAAACCAGCACAACCCCCTTTGGCTCTGCAAATGCCAACGCAGCCTCTCACCCCCTATGCCCA
  GATCATGCAGTCGCTCCAGCTGTCCAGTCCCACCCTGTCCCCACAGGACCCCGCTGTGCCTCCATCAGAG
  CCCAAGCCCCACGCGGCTGTGTTTGCCAGGGTGGCTTCCCCACCACCCGGAGTCTCTGAGAAGCGCGTGC
  CTTCGGCCAGAACTCCCCCAGTGCTAGCCGAGAAAGCCCGAGTCCCCACGGTGCCCCCCAGGCCAGGCAG
  CAGCCTCAGCGGCAGCATCGAAAACCTTGAATCAGAGGCTGTGTTCGAGGCTAAGTTCAAACGCAGCCGC
  GAATCGCCACTGTCGCGGGGCCTGAGGCTACTGAGCCGCTCGCGCTCGGAGGAGCGCGGTCCCTTCCGTG
  GGGCGGAGGACGACGGCATATACCGGCCCAGTCCGGCAGGAACGCCGCTAGAGCTGGTGCGACGGCCCGA
  GCGCTCGCGCTCCGTGCAGGACCTCAGGGTAGCCGGAGAGCCGGGCCTTGTGCGTCGCCTTTCGCTGTCC
  CTGTCGCAGAAGCTGCGGCGGACACCGCCGGGACAGCGACACCCTGCCTGGGAGTCCCGCAGCGGAGACG
  GGGAGAGCTCCGAGGGCGGCAGCTCAGCTCGGGCATCCCCAGTGCTGGCGGTACGCAGGAGACTCAGCTC
  CACGCTGGAGCGTCTGTCCAGCCGTTTGCAGCGCAGCGGCAGCAGCGAGGACTCCGGGGGCGCCTCGGGT
  CGCAGCACGCCGCTGTTTGGACGGCTGCGCAGGGCTACTTCGGAAGGGGAGAGCCTGCGACGCCTCGGTG
  TTCCGCACAATCAGCTGGGCTCTCAGACTGGTGCCACTACACCTTCCGCTGAGTCTCTGGGCTCAGAGGC
  CAGTGGCACATCGGGTTCTTCAGCTCCTGGAGAAAGCCGAAGCCGGCACCGGTGGGGCCTTTCCAGGCTG
  CGCAAGGACAAGGGATTATCTCAGCCAAACCTCTCCTCCAGTGTCCAAGAGGACTTGGGTCACCAGTATG
  TGCCCAGTGAGTCAGATTTCCCCCCAGTCTTCCACATCAAACTCAAGGACCAGGTGCTGTTGGAGGGGGA
  AGCAGCCACCCTGCTCTGCCTACCCGCAGCCTGCCCAGCACCCCGCATCTCCTGGATGAAAGATAAGCAG
  TCCCTGCGTTCAGAGCCCTCAGTGGTCATTGTGTCCTGCAAAGATGGACGGCAGCTGCTGAGCATACCCC
  GGGCCGGCAAGCGGCATGCTGGGCTCTATGAGTGCTCAGCCACCAATGTCCTGGGCAGCATTACCAGCTC
  CTGTACTGTGGCCGTGGCCCGCATTCCAGGGAAGCTAGCTCCTCCAGAGGTGCCCCAGACCTACCATGAC
  ACAGCACTAGTAGTGTGGAAGCCAGGAGACGGTCGGGCACCTTGCACATACACTCTGGAGCGGCGGGTGG
  ATGGGGAATCTGTCTGGCACCCTGTGAGCTCAGGCATCCCCGACTGTTATTACAATGTGACTCAGCTGCC
  TGTCGGCGTGACCGTGCGGTTCCGTGTGGCCTGTTCCAATCGTGCTGGGCAGGGGCCCTTCAGCAACCCT
  TCTGAGAAGGTCTTCATCCGAGGCACTCCAGATTCTCCTGCTCAGCCAGCTGCTGCTCCCCGAGATGCCC
  CTGTTACCTCAGGGCCAACCAGGGCCCCACCGCCTGACTCTCCTACCTCACTGGCCCCCACCCCCGCTCT
  TGCGCCCCCAGCCTCCCAGGCATCCACTCTCAGCCCCTCAACTTCCTCCATGTCTGCCAACCAGGCCTTG
  TCCTCACTCAAGGCTGTGGGTCCACCACCCGCAACCCCTCCACGAAAGCACAGGGGCCTTCTGGCCACCC
  AGCAAGCAGAGCCATCCCCACCCAGTATCGTGGTCACCCCAAGTGAACCCAGGTCTTTTGTCCCTGACAC
  TGGAACCCTGACCCCAACCTCTAGTCCTCAAGGGGTTAAACCAGCACCTTCCTCAACTTCCTTGTATATG
  GTGACTTCCTTCGTGTCTGCACCTCCAGCCCCCCAGGCCCCAGCCCCTGAGCCCCCTCCTGAGCCCACCA
  AGGTGACTGTGCGGAGCCTCAGCCCAGCCAAGGAGGTGGTCAGCTCCCCAACTCCTGAGAGCACCACTCT
  TCGGCAGGGTCCCCCTCAGAAACCCTACACCTTCCTGGAGGAGAAGGCCAGGGGCCGCTTTGGCGTTGTG
  CGGTCATGCCGGGAGAATGCTACGGGCCGAACGTTTGTCGCCAAGATCGTGCCGTACGCCGCGGAGGGCA
  AGCGACGCGTGCTGCAGGAATACGAGGTGCTTCGGACGCTGCACCACGAGCGGCTCATGTCCCTGCACGA
  GGCCTACATCACCCCTCGGTACCTCGTGCTTATCGCTGAGAGCTGTGGCAACCGGGAACTCCTCTGTGGG
  CTCAGCGACAGATTCCGGTATTCAGAAGATGACGTGGCCACCTATGTGGTGCAGCTGCTACAAGGCCTGG
  ACTACCTCCACGGCCACCACGTGCTACACCTGGACATCAAGCCGGACAATCTACTGTTGGCCGCTGACAA
  TGCCCTTAAAATTGTGGACTTCGGCAGTGCTCAGCCCTATAACCCTCAGGCCCTGAAGCCACTTGGCCAC
  CGCACAGGCACTCTGGAGTTCATGGCTCCTGAGATGGTGAAGGGAGACCCCATTGGCTCTGCCACGGACA
  TCTGGGGAGCGGGTGTGCTCACCTACATCATGCTTAGTGGGTACTCCCCATTCTATGAGCCAGACCCCCA
  GGAAACAGAGGCTCGGATTGTTGGGGGTCGCTTTGATGCCTTCCAGTTGTATCCTAACACATCCCAGAGT
  GCCACCCTCTTCTTGAGAAAGGTCCTCTCAGTACATCCCTGGAGCCGGCCCTCTCTGCAGGACTGCTTGG
  CCCACCCATGGCTGCAAGATGCCTACCTGATGAAGCTGCGCCGCCAGACACTCACCTTCACCACCAACCG
  GCTCAAGGAATTCCTGGGCGAGCAGCGGCGACGTCGGGCTGAGGCTGCTACCCGTCACAAGGTGCTGCTC
  CGCTCCTACCCTGGCAGCCCCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001085370
• Insert Size: 7584 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085370.1, NP_001078839.1
• RefSeq Size: 9885 bp
• RefSeq ORF: 7584 bp
• Locus ID: 11790
• UniProt ID: Q62407
• Cytogenetics: 1 38.88 cM
• Gene Summary: This gene encodes a protein with similarity to members of the myosin light chain kinase family. This protein family is required for myocyte cytoskeletal development. Studies have determined that a lack of this protein affected myocardial development. Multiple alternatively spliced transcript variants that encode different protein isoforms have been defined. [provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. These differences produce a unique 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. This variant is similar, but not identical, to variant SPEGalpha described in PubMed ID: 10973969.