Cenpe (NM_173762) Mouse Untagged Clone

CAT#: MC225329

Cenpe (untagged) - Mouse centromere protein E (Cenpe), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cenpe
• Synonyms: 312kDa; AU019344; BC049989; C530022J18; CENP-E; Kif10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225329 representing NM_173762
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGAGGCTTCAGTGGCTGTCTGTGTTCGTGTGCGACCCCTCAACAGCAGAGAAGAAGAACTTG
  GAGAAGCCACCCACATTTACTGGAAAACAGATAAAAACGCTATTTATCAGAGCGATGGGGGTAAATCTTT
  CCAATTTGATCGTGTCTTTGACAGTAATGAAACCACGAAGAATGTGTATGAAGAGATTGCGGTGCCCATC
  ATCAGCTCCGCCATACAAGGCTACAATGGTACTATATTTGCCTATGGACAAACTGCATCAGGAAAGACAC
  ACACGATGATGGGTTCTGAAGATTGTTTGGGAGTTATACCCAGGGCAATTCATGACATTTTCCAAAGGAT
  AAAGAAGTTTCCTGAGAGAGAATTTCTCCTACGAGTTTCTTACATGGAGATCTACAATGAAACCATTACA
  GATTTACTCTGTAATGCTCAAAAAATGAAACCTTTGATAATTCGGGAAGACACCAATAGGACTGTATATG
  TTTCTGATCTCACAGAAGAAGTGGTTTATACAGCAGAAATGGCTTTGAAATGGCTCGCAACAGGAGAAAA
  AAACAGACATTATGGAATAACCAAAATGAATCAAAGAAGCAGTCGTTCTCATACGATTTTTAGGATGATT
  CTGGAAAGTAGAGAAAAGGCTGAGCCTTCTAACTGCGATGGCTCCGTCAAAGTGTCTCATCTGAATTTGG
  TCGATCTTGCAGGCAGTGAAAGAGCTGCTCAAACAGGAGCTGAAGGTGTGCGATTGAAGGAGGGCTGTTT
  TATAAATCGGAACTTGTTTATTTTGGGACAAGTGATTAAGAAACTCAGTGATGGACAGGTTGGGGGTTTC
  ATAAATTACCGAGATAGCAAATTGACACGAATTCTTCAGAATTCCTTGGGAGGAAATGCCAAGACGCGCA
  TCATCTGCACGATCACCCCAGCGTCCCTGGACGAAACCCTGACGACGCTGCAGTTTGCCAGCACTGCTAA
  GTATATGAAGAATACTCCTTACGTTAATGAAGTGTCGAATGATGAAGCTCTCTTGAAAAGATACAGAAGA
  GAAATAGCGGATCTTAGAAAACAATTAGAGGAGGTAAATACAAAGACTCGGGCACAGGAAATGGAAAAGG
  ATCAACTGGCCCAGCTTCTGGATGAAAAAGACTTGCTTCAGAAAGTTCAGGATGAGAAAATTAATAACCT
  GAAGCGGATGCTGGTGACGTCATCTTCTATTGCATTGCAGCAGGAGCTAGAGATTAAAAGGAAACGAAGA
  GTTACTTGGTGCTATGGCAAAATGAAGGATTCAAACTATGAAAAGGAATTTAAAGTCCCGACAAGCATAA
  CAACAAGAAAACGTAAAACTTCTGTGACTTCTTTGAGAGAAAATTCTCTCATGAAGTTCGGAGAGAGTGC
  AGCCTCTTCCGAGTTTGAAATGTTGAATAACACTCTTGAGTCATTGGCTGAGGTAGAGTGGAGCTCGGCA
  ACAACGCTCCTAAGCGAGGAAAATGTAGAAAGTGAGTTGAACTCACTTAATGCTCAATATAATGATCTGG
  TATTAGACTATGAACAGCTCAGAAGAGAAAATGAAGACTTGAAATTGAAATTAAAAGAAAAAAATGAGTT
  AGAGGAATTTGAGCTTCTAGAACAAAAAGAAGAAAGAGATCAAGAGATGCAACTAATGCATGAAGTTTCC
  AACTTAAAGAATTTAATTAAACACGCAGAAGAATATAATCAAGACCTTGAGAATGACCTAAGTTCAAAAG
  TGAAGCTTCTTAAAGAAAAAGAGGAGCAGATTAAGAACCTGCAGGAATACATAGATGCCCAGAAGTCAGA
  AAAGATGAAGATAGACCTGTCATACACATCGGACGCCACTGAAGACCTGAAACAGGCGATGCGCACCCTG
  TCTGATTTGGACACAGTGGCCCTTGACGCTAAGAAAGAGTCAGCCTTCCTTAGAAGTGAAAATCTGGAGC
  TGAAGGAGAAAATTAATGAACTTTCGGATTCCCGTAAGCAAATGGAAAGTGACATTCAGATGTATCAACG
  CCAACTGGAAGCAAAAAAGAAAATGCAAACTGATCTGGATAAGGAATTACAGTTGGCTTTTCAAGAAATA
  TCAAAACTCAGCGCCCTGGTAGATGGCAAAGGTTTACTGTCTAATTTGGAATTGGAGAAAAGGATTACTG
  ATCTCCAAAAAGAACTAAATAAAGAAGCTGAAGAAAAACAAACTTTGCAAGAAGAAGTTAACTTGCTGTC
  GGAGTTGAAATCTTTACCGTCTGAGGTGGAAACGCTGAGGAGAGAGCTGTATGAGAAATCTGAAGAGCTC
  CATATAATAACCACAGAAAGAGAGAAACTGTTTTCTGAAATGGCTCATAAAGACAGTAGGATTCAAGGTT
  TACTTGAGGAAATTGGAAACACTAGAGATGACCTTGCCACTTCCCAGCTGAGTCGGAGGGGCAGTGATGG
  AGAATGGCAAGCCCTGGAAAGCCTGCACGCAGAGCTGGAGCACAGACACGCGGGGGTCCTGGAGGAGAGA
  GAGAGGCTGAAGCAGGAGATTGGGGCTCTCTCCAAAGAAGCAGAAAGCCTGGCTTTCAGTTTGGATTCCG
  TGAAGGCCGAGCTTTCTCACAAGACTCAAGAGCTTGAGCAGAAAACAGTTGAGGGTCAAGAAAGGCTAAA
  TAAAATGGAGGCGCTGAGAGAAGAGTTAGAAAGCAGAGACTCCAGCCTGCAGTCTGTAGAGAAGGAGAAA
  GTCCTGCTCACTGAGAAGCTGCAGCAAGCCTTGAAGGAAGTAAAAGCCTTAACCCAAGAAAAGAAAAACC
  TGAAGCAGCTCCAGGAGAGCCTGCAAACTGAGAGAGACCAACTCAGAAGTGACATTCAGGACACTGTGAA
  CATGAACATAGATACTCAAGAGCAGTTACTAAATGCTCTGGAGTCTCTGAAACAACACCAAGAAACAATT
  AACATGCTGAAAATGAAAGCTGCTGAGGAACTATCCGATAATTTGCATGTAAAGGACAGGGGAGGAGCTA
  GAGATGAAGCTCAGCAAAAGATGGATGGCATAGATGAACAGAATGAGAGTGCCCACACATTGCTTGGTGG
  TGGTAAGGACAATGAGGTAACTGAGGAACAGAGGAAAATCGACTCTCTAATGCAGGAGAATAGCGGACTC
  CAACAGACACTGGAAAGTGTTAGAGCAGAAAAGGAACAACTGAAGATGGACCTAAAGGAAAATATCGAAA
  TGAGTATTGAAAACCAGGAAGAGTTAAGAATTCTTAGAGATGAACTGAAAAGACAACAAGAGGTAGCTGC
  ACAGGAAAAGGATCATGCCACCGAGAAGACCCAGGAGCTCTCCAGGACGCAGGAAAGATTGGCAAAAACA
  GAAGAGAAGTTAGAGGAAAAGAACCAGAAACTCCAAGAGACACAGCAGCAACTTCTTAGTACACAAGAAG
  CTATGAGTAAGCTGCAGGCAAAGGTGATTGACATGGAAAGCTTACAGAATGAATTCAGGAACCAAGGATT
  GGCATTGGAGCGTGTGGAGACAGAGAAACTGGAGTTAGCTCAGAGACTTCATGAAAGTTATGAGGAGGTG
  AAATCTATAACCAAAGAAAGAAATGATCTAAAGGAATTGCAGGAGTCATTTGAAATAGAGAAGAAGCAAC
  TTAAAGAATATGCAAGGGAAATAGAAGCCGCGGGCCTACAAACAAAAGAAGAACTAAACATTGCCCACGC
  GAATCTAAAAGAGTACCAGGAAATCATCACAGAGCTAAGAGGAAGCATTTCTGAAAACGAAGCACAAGGA
  GCAAGCACCCAGGACACGGCAAAAAGCGCCCCTGAGCTACAAGGAGAGGTTCCAGAGCAAGAGCTGCTTC
  CTGTTGTCAAAGAAGCCAGGCACAGTGCAGAAAAAGTGAACGGACTGGAGCCAGTGGGAGCACATTCCAG
  AACAGTGCACTCCATGACAATGGAGGGCATAGAAATAGAAAACCTCAGGCTGACCAAGAAACTTGAAGAA
  AGTCAGATGGAGATCAGCTGTCTCACTAGAGAGAGAGAAGACCTGAGAAGGACACAGGAAACACTTCAGG
  TCGAATGTACCCAGCTGAAAGAAGATGCCAGAAGAACTTTGGCTAATCATCTAGAAACTGAAGAGGAACT
  TAACCTTGCTCGTTGTTGCCTGAAAGAGCAGGAGAACAAGATTGACACCCTAATAACGAGCCTCTCGCAG
  AGGGAAACTGAGCTGTCCAGTGTGCGAGGACAACTAGCGCTGACTACTGCTGAACTGGAGAGAAAGGTTC
  AAGAACTCTGTGAGAAACAGGAAGAACTTACTAGGAAAGAAACTAGCGAGGCTCAAGGGAAGATGAGTGA
  ACTAGAGCAACTCAGGGAGCTGCTGCTTGCCCAGGCCTCGGCACTGCAGAACGCAGAGAGTGACAGGCTG
  CGGCTGAATACACAACTGGAAGAAAGTCAAGAGGAAATGAAAACTCTAAGGGAGGAAAGAGAAGAGCTGA
  GAAGAATGCAAGAGGCTCTTCACGTGGAAAGTGAGCAGCAGAAAGAAAGCATGAAGGAAATCAGCTCAAA
  ACTCCAAGAACTACAGAACAAAGAGTATGAATGTCTTGCAATGAAGACTATTAATGAGACTCAAGGAAGC
  AGATGTGAAATGGACCACTTGAACCAGCAGCTGGAGGCCCAGAAGTCAACTCTGGAAAAGGTGGAGATGG
  AGAATGTAAACTTGACTCAAAGACTTCATGAGACCCTTGAGGAAATGAGATCTGTAGCAAAAGAAAGAGA
  TGAGCTTTGGAGCATGGAGGAGCGCCTCACAGTAGAGAGAGACCAACTGAAGAAGAGCCTTGAAGAAACA
  GTAACTAAAGGTATGGAAAAAGAGGAAGAGCTAAGAGTTGCGCATGTGCATCTGGAAGAGCACCAGGAAA
  CTATCAACAAGCTCAGAAAGATGGTGTCTGACTACACAGATGAAATATCACATACTCAAGGGGACCTAAA
  GCACACAAATGCCGTTGTAGAAGCTCAGAACCAAGATCTCCGGGAAAAAGAACATCAACTTTCTCAAGTA
  AAAGCTGATCTCAGAGAAACCGTGGATCAAATGGAGCAACTGAAGAAGAAGCTCGAAGCCCAGAGTTCGA
  CTCTAGAAAGCAGAGAAATAGAGAAGTTAGAATTGACTCAGCAACTTAACGAAAACCTTAAAAAAATAAC
  ACTTGTCACTAAGGAAAATGATAGCCTAAAAATTATGGATGAAGCCCTCAGGGAAGAACGAGACCAGCTA
  CGGAAAAGTCTTCAGCAAACAGAGGCAAGGGATCTGGAAAATCAAGAGAAACTAAGAATTGCTCACATGA
  ATCTAAAAGAGCACCAAGAAACTATTGACCGACTCATGGAGACTATGTCTGAGAAGACAGAGGAAATATC
  AAATATGAAAATGGAGTTAGAAAATGTAAACATGAAATTACAAGAAAAGGTTCAAGAACTTAAGACAAGT
  GAGCGTCAACGTGTTAAGTTAAAAGCAGATGCCAGTGAAGCCAAGAAGGAGCTCAAGGAGCAGGGCTTAA
  CTCTGAGTAAGATAGAGATGGAGAACTTAAATCTCGCTCAGAAGATTCATGAGAACCTTGAAGAAATGAA
  ATCTGTAAGAAAAGAAAGAGATGATCTAAAGAAACTTGAGGAGATACTCAGAATGGAGAGAGACCAGCTC
  AAGGACAACCTGAGAGAAGCCATGCTTAAGGCCCACCAGAACCATGAAGAGACAATGAAATGTGGAAAGG
  GGTTACTGTGTGCTGGAGAATACTGTACAGGAAGACTTCGAGAAAAGTGCTTCAGGATAGAAAAACTCCT
  GAAAAGATACTCAGAAATGGCTAATGATTATGAGTGCTTGAATAAAGTCTCTCTTGACTTGGAGAGAGAA
  ACTAAAACCCAAAAAGAGCTGTCAGTTACTGTAAGAACAAAGCTCTCACTTCCGCACACACAGACCAAAG
  AGATGGAAAAGCTTCTGACTGCAAACCAGAGATGTTCCCTGGAGTTCCACAGAGCCCTCAAGAGACTTAA
  GTACGTGTTAAGCTCCATTGCGAGGATAAAGGAAGAGCAGCATGAGTCCATCAATAAACGTGAAATGGCT
  TTTATTCAGGAAGTGGAAAAGCAGAATGAACTGCAGATTCAAATCCAGAGCCTTTCACAGACTTACCGTA
  TTCCAGCCAGAGACTTACAGATCAAACTGAGCCAGGAGATGGACCTCCATATTGAGGAAATGCTCAAAGA
  TTTTTCAGAAAATGATTTCCTCACCATAAAAACTGAAGTCCAACAAGTATTAAATAATAGGAAAGAAATA
  ACTGAGTTTTTGGGAAAATGGCTAAATACTCTTTTTGATACAGAAAATCTTAAGAGCACCATCCAGAAAG
  AAAACAAAAGCATTGGGCTCGTGAATAACTTCTATCACAGCAGAATCACTGCCATGATAAATGAATCAAC
  GGAGTTTGAGGAAAGAAGTGCTACCAGATCCAAAGACTTGGATCAGTACCTGAAGTCACTGAAAGAAACA
  ACTGAGCAACTGTCTGAAGTCTACCAGACACTGACCGCCTCCCAGTCTGTTGTGCATCTCCATCCTACCG
  TGCAGCCTTCCACACGGGACAGTGAGCGTCCTCAGGCTGCATCAGGAGCTGAACAACTGACCTCAAAGAA
  CAAAATTGCTTTAGGAGCTGTGCCCTATAAAGAAGAAATTGAGGATCTCAAAATGCAGCTGGTGAAGAGT
  GACCTAGAGAAGAAGGCGACCGCCAAGGAATTCGATAAGAAAATTCTCTCTCTAAAAGCCACTGTTGAAC
  ATCAAGAAGAAATGATAAGGCTACTGAGAGAAAATCTTAGAGGACATCAACAGGCCCAGGATACCTCAAT
  GATCTCAGAACAAGACTCTCAGCTTCTGAGTAAACCTCTAACCTGTGGAGGTGGCAGTGGAATTGTACAA
  AGCACAAAAGCTCTTATTTTGAAAAGTGAATATAAAAGGATGGGAAGTGAGATTTCTAAGTTAAAGCAGC
  AAAATGAGCAGCTAAGAAAGCAAAATAATCAACTATTAAGTGATAACTCTCAACTCTCCAATGAGGTTAA
  AACCTGGGAGGAAAGAACTCTTAAAAGAGACAGTTACAGAGAAACAACTTGTGAGAATTCTCCAAAGTCT
  CCTAAAGTGACTCGGACAGATTCTAAAAGGAGACAGAACACAACTTCTCAGTGCAGGGCACAAAATCTCC
  AGGATCCTGTGCCAAAAGATTCCCCAAAATCATGGTTTTTTGATAACCGGTCAAAGTCTCTTCCAGCACC
  TCACCCGATCCGCTATTTTGACAACTCCAGTCTAGGGCTGTGCCCAGAACCAGATGACGTAGAGAATGTG
  GAGCCTAAGACAGACCTCTGCCAAGCCTCCTTGGAAAAAGATGTGTCCCAGTGCAAGACGCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173762
• Insert Size: 7416 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173762.4, NP_776123.3
• RefSeq Size: 7813 bp
• RefSeq ORF: 7416 bp
• Locus ID: 229841
• UniProt ID: Q6RT24
• Cytogenetics: 3 62.55 cM
• Gene Summary: Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (By similarity). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:12925705). The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC (PubMed:12361599). Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (PubMed:12925705).[UniProtKB/Swiss-Prot Function]