Notch2 (NM_010928) Mouse Untagged Clone

CAT#: MC225328

Notch2 (untagged) - Mouse Notch gene homolog 2 (Drosophila) (Notch2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Notch2
• Synonyms: AI853703; N2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225328 representing NM_010928
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGCTCTGCGTCCCGCCGCGCTGCGGGCGCTGCTGTGGCTCTGGCTGTGCGGCGCGGGCCCCGCGC
  ACGCTTTGCAGTGTCGAGGTGGTCAAGAGCCCTGTGTAAATGAAGGGACCTGTGTTACCTACCACAACGG
  CACAGGCTTCTGCCGATGTCCAGAGGGCTTCTTGGGAGAATATTGCCAACATCGAGACCCCTGTGAGAAA
  AACCGCTGTCAGAATGGTGGTACTTGTGTGCCGCAGGGCATGTTGGGGAAAGCTACCTGCCGATGTGCTC
  CAGGATTCACAGGAGAGGACTGCCAATACTCCACCTCTCACCCCTGCTTTGTGTCCCGCCCTTGTCAGAA
  TGGGGGTACCTGTCACATGCTCAGCCGGGACACCTACGAGTGCACCTGCCAAGTCGGCTTCACAGGGAAG
  CAGTGTCAGTGGACAGATGCCTGTCTATCTCATCCCTGCGAAAATGGAAGCACCTGTACATCTGTGGCCA
  GCCAGTTCTCCTGCAAATGCCCTGCAGGCCTCACAGGGCAGAAGTGTGAAGCTGATATCAATGAGTGTGA
  CATTCCAGGACGCTGCCAACATGGTGGCACCTGCCTCAACCTTCCTGGTTCCTACAGATGCCAATGTCCT
  CAGGGCTTCACAGGCCAGCACTGTGACAGCCCTTATGTGCCCTGTGCACCCTCGCCCTGCGTCAACGGAG
  GCACCTGTCGTCAGACTGGCGACTTCACTTTCGAATGCAACTGCCTGCCAGGTTTTGAAGGGAGCACCTG
  TGAGCGGAATATCGACGACTGCCCCAACCACAAGTGTCAGAATGGAGGAGTTTGTGTGGATGGTGTCAAT
  ACCTACAATTGCCGCTGTCCCCCTCAGTGGACTGGGCAGTTCTGCACAGAAGATGTGGACGAGTGTCTGT
  TGCAGCCCAACGCTTGTCAGAATGGAGGCACCTGCACCAACCGAAACGGAGGCTATGGCTGCGTGTGTGT
  GAACGGCTGGAGCGGAGATGACTGCAGCGAGAATATCGATGACTGTGCCTATGCTTCCTGCACGCCAGGG
  TCCACCTGCATTGACCGCGTGGCCTCCTTCTCCTGCCTGTGTCCGGAGGGAAAGGCGGGTCTCCTGTGCC
  ATCTGGATGATGCCTGTATCAGCAACCCTTGTCACAAGGGGGCGCTGTGTGACACCAACCCCCTGAACGG
  GCAGTACATTTGCACCTGCCCACAGGGCTACAAGGGCGCTGACTGCACAGAAGACGTGGATGAGTGTGCT
  ATGGCCAACAGTAACCCTTGTGAGCATGCAGGAAAGTGTGTGAATACAGATGGCGCCTTCCACTGTGAGT
  GTCTGAAGGGCTACGCAGGGCCTCGCTGTGAGATGGACATCAACGAGTGTCATTCAGACCCCTGCCAGAA
  CGACGCCACCTGCCTGGATAAGATCGGAGGCTTCACCTGTCTGTGCATGCCAGGTTTCAAAGGTGTGCAT
  TGTGAGCTGGAGGTAAATGAATGCCAGAGCAACCCGTGTGTGAACAACGGGCAGTGTGTGGACAAAGTCA
  ACCGCTTCCAGTGTCTGTGTCCCCCTGGCTTCACAGGACCAGTGTGCCAGATCGACATTGATGACTGCTC
  CAGTACTCCCTGCCTGAATGGGGCCAAGTGCATCGATCACCCGAATGGCTATGAATGCCAGTGTGCCACA
  GGTTTCACTGGCATATTGTGTGATGAGAACATCGACAACTGTGACCCAGATCCTTGCCACCATGGCCAGT
  GCCAGGATGGGATCGACTCCTACACCTGCATCTGCAACCCTGGGTACATGGGAGCCATCTGTAGTGACCA
  GATTGATGAATGCTACAGCAGCCCTTGCTTGAACGATGGGCGCTGCATTGACCTGGTGAATGGTTACCAG
  TGCAACTGCCAACCAGGCACATCAGGCCTTAATTGTGAAATTAATTTTGATGACTGTGCCAGCAACCCTT
  GTATGCACGGAGTCTGTGTGGACGGCATCAATCGCTACAGCTGTGTGTGCTCTCCGGGATTCACAGGCCA
  GAGGTGCAATATTGACATTGATGAGTGTGCCTCCAACCCCTGTCGCAAGGGTGCGACGTGCATCAATGAT
  GTGAATGGTTTCCGGTGTATATGCCCCGAGGGACCGCATCATCCTAGCTGCTACTCACAGGTGAACGAGT
  GCCTGAGCAATCCCTGCATCCACGGAAACTGTACTGGAGGTCTCAGTGGCTATAAGTGCCTCTGCGATGC
  AGGCTGGGTTGGTGTCAACTGTGAGGTGGACAAAAATGAGTGTCTTTCTAACCCATGCCAGAATGGAGGA
  ACGTGCAATAACCTGGTGAATGGCTACAGGTGTACCTGCAAGAAGGGGTTCAAAGGCTACAACTGCCAGG
  TGAATATAGATGAGTGTGCCTCCAACCCGTGTCTGAACCAAGGAACCTGCTTTGATGACGTCAGTGGCTA
  CACTTGCCACTGCATGTTGCCTTACACAGGCAAGAACTGTCAAACAGTGTTGGCTCCCTGTTCCCCAAAC
  CCGTGTGAGAATGCTGCTGTTTGTAAAGAGGCACCCAATTTTGAGAGCTTCAGCTGCTTGTGTGCTCCTG
  GCTGGCAAGGTAAACGATGTACAGTTGACGTTGATGAGTGTATCTCCAAGCCGTGTATGAACAATGGTGT
  CTGCCACAACACTCAGGGCAGCTACGTGTGTGAGTGTCCTCCCGGCTTCAGTGGGATGGACTGTGAAGAG
  GACATCAATGATTGCCTTGCCAACCCCTGCCAGAACGGAGGCTCCTGTGTGGACCATGTGAATACCTTCT
  CCTGCCAGTGCCATCCTGGCTTCATAGGGGACAAGTGCCAGACAGACATGAATGAGTGTCTGAGCGAGCC
  CTGTAAGAATGGGGGGACCTGCTCCGACTATGTCAACAGTTACACCTGCACATGCCCTGCGGGCTTCCAC
  GGAGTCCACTGTGAAAACAACATTGACGAGTGCACTGAGAGCTCCTGCTTCAATGGCGGCACGTGTGTTG
  ATGGAATCAACTCTTTCTCTTGCTTGTGCCCCGTGGGTTTCACTGGTCCCTTCTGCCTCCATGATATCAA
  TGAGTGCAGCTCTAACCCGTGCCTGAATGCTGGAACGTGTGTTGATGGTCTGGGTACCTACCGATGCATC
  TGTCCCTTGGGCTACACCGGGAAAAACTGTCAGACCCTGGTGAACCTCTGCAGCCGGTCTCCGTGTAAAA
  ACAAAGGAACTTGTGTTCAGGAAAAGGCAAGGCCCCACTGTCTGTGTCCGCCTGGATGGGATGGTGCATA
  CTGTGATGTGCTCAACGTGTCCTGTAAGGCGGCAGCCTTGCAGAAAGGAGTACCTGTTGAACACTTGTGC
  CAGCACTCGGGCATCTGTATCAATGCTGGCAACACCCATCACTGCCAGTGCCCCCTGGGCTACACGGGGA
  GCTACTGCGAGGAGCAGCTTGATGAGTGTGCATCCAATCCATGCCAGCACGGTGCCACCTGCAATGACTT
  CATCGGAGGATACAGATGTGAGTGTGTTCCAGGATATCAGGGTGTCAACTGTGAGTATGAAGTGGACGAG
  TGCCAGAACCAACCCTGCCAGAATGGAGGCACCTGCATCGACCTTGTGAACCATTTCAAGTGTTCGTGTC
  CCCCAGGCACCCGGGGCCTGCTGTGTGAAGAGAACATCGATGAATGTGCTGGGGGCCCCCACTGCCTTAA
  CGGTGGCCAGTGCGTGGACCGGATTGGAGGCTACACTTGTCGCTGTTTGCCTGGCTTTGCTGGGGAGCGG
  TGTGAGGGGGACATCAACGAATGCCTGTCCAACCCTTGCAGCTCAGAGGGCAGCCTGGACTGCGTTCAGC
  TCAAAAATAACTACAACTGTATCTGCCGCAGCGCCTTCACGGGCCGGCACTGTGAAACCTTCCTAGATGT
  GTGTCCCCAGAAGCCTTGCCTGAACGGAGGGACTTGTGCCGTGGCTAGCAACATGCCCGATGGCTTCATC
  TGTCGTTGTCCCCCAGGGTTCTCTGGGGCGAGATGCCAGAGCAGCTGTGGACAAGTGAAGTGCAGGAGAG
  GGGAGCAGTGTATCCACACTGACTCGGGACCTCGCTGCTTCTGCCTGAACCCCAAGGACTGCGAGTCAGG
  ATGTGCCAGTAACCCCTGCCAGCATGGGGGCACCTGCTATCCTCAGCGCCAGCCTCCTCACTATTCCTGC
  CGCTGCCCTCCGTCGTTCGGGGGCAGCCACTGCGAACTCTACACAGCCCCCACCAGCACCCCTCCTGCTA
  CCTGTCAGAGTCAGTACTGCGCCGACAAGGCTCGGGATGGCATCTGTGACGAGGCCTGCAATAGTCATGC
  CTGCCAGTGGGATGGAGGTGACTGTTCCCTCACTATGGAGGACCCCTGGGCCAACTGCACCTCCACTCTT
  CGCTGCTGGGAATACATCAACAACCAGTGTGATGAGCAGTGCAACACCGCAGAGTGCCTGTTTGACAACT
  TTGAGTGCCAGAGGAATAGCAAGACGTGCAAGTATGACAAATACTGTGCAGACCACTTCAAAGACAACCA
  CTGTGATCAGGGATGCAACAGCGAGGAGTGTGGCTGGGATGGGCTGGACTGCGCTTCGGACCAGCCTGAG
  AACCTGGCAGAGGGCACCCTGATCATCGTGGTGCTCCTGCCCCCTGAACAGCTGCTGCAGGATTCGCGAA
  GCTTCTTGAGGGCCCTGGGCACCCTGCTCCACACCAACTTGCGCATTAAACAAGATTCTCAGGGCGCTCT
  CATGGTGTACCCCTACTTTGGGGAGAAGTCAGCTGCCATGAAGAAGCAGAAGATGACACGCAGGTCTCTT
  CCTGAGGAGCAGGAGCAGGAGCAGGAGGTGATAGGCTCTAAGATATTTCTGGAGATCGACAACCGACAGT
  GTGTTCAAGATTCAGACCAGTGCTTCAAAAACACAGATGCGGCAGCTGCTCTCCTGGCTTCTCATGCCAT
  CCAAGGGACCCTATCCTACCCTCTAGTGTCTGTTTTCAGTGAACTGGAGAGTCCAAGAAACGCCCAGCTT
  CTCTATCTGCTCGCGGTCGCTGTTGTCATCATCCTGTTCTTCATCCTGCTGGGGGTCATCATGGCCAAGC
  GGAAGCGCAAGCATGGCTTCCTCTGGCTGCCGGAAGGGTTCACCCTCCGCCGAGACTCTAGCAATCACAA
  GCGCCGTGAACCTGTGGGACAGGATGCCGTGGGGCTGAAAAATCTCTCCGTGCAAGTGTCAGAGGCTAAC
  CTGATTGGTTCTGGGACAAGTGAACATTGGGTTGATGATGAAGGACCCCAGCCAAAGAAAGCCAAGGCTG
  AGGATGAGGCTTTGCTGTCGGAAGATGACCCCATCGATCGACGGCCCTGGACACAGCAGCACCTTGAAGC
  TGCAGACATCCGCCACACTCCATCCCTGGCACTCACTCCTCCTCAGGCAGAACAGGAGGTGGACGTGCTG
  GACGTGAATGTCCGAGGCCCAGATGGGTGTACTCCACTGATGCTGGCTTCTCTCCGAGGAGGCAGCTCAG
  ACCTGAGTGATGAAGACGAAGATGCTGAGGACTCTTCTGCCAACATCATCACAGACTTGGTCTACCAAGG
  TGCCAGCCTTCAAGCACAGACAGACCGCACTGGCGAGATGGCCCTGCACCTTGCAGCCCGCTATTCGAGA
  GCTGATGCTGCCAAACGCCTCCTGGATGCTGGTGCGGATGCAAATGCCCAGGACAACATGGGCCGATGTC
  CTCTTCACGCTGCGGTGGCAGCAGACGCCCAAGGTGTCTTTCAGATTCTGATCCGCAACCGTGTAACCGA
  TCTGGATGCCAGAATGAACGATGGTACTACCCCCCTGATCCTGGCTGCCCGCCTGGCTGTGGAAGGAATG
  GTGGCAGAGTTGATCAATTGCCAAGCAGATGTCAATGCAGTGGATGACCATGGAAAATCTGCCCTCCACT
  GGGCAGCTGCTGTCAATAATGTGGAGGCGACTCTTCTGCTGTTGAAGAATGGGGCCAACAGAGATATGCA
  GGACAATAAGGAAGAGACACCTTTGTTTCTTGCTGCCCGAGAGGGAAGTTATGAAGCGGCCAAAATCCTG
  TTAGACCATTTTGCCAACCGGGACATCACTGACCACATGGACCGCCTTCCCCGGGATGTGGCTCGGGACC
  GCATGCACCATGACATCGTTCGCCTCCTGGACGAGTATAACGTGACTCCCAGCCCTCCGGGAACGGTCTT
  GACTTCTGCGCTCTCACCTGTCCTCTGTGGGCCCAACAGGTCTTTCCTCAGTCTGAAGCACACCCCAATG
  GGTAAGAAGGCTAGGCGGCCCAACACCAAGAGCACCATGCCCACGAGCCTGCCTAACCTTGCCAAGGAGG
  CCAAGGATGCCAAGGGCAGCAGGAGGAAGAAGTGTCTGAACGAGAAGGTCCAGCTGTCCGAGAGCTCAGT
  GACTCTATCCCCCGTCGATTCGCTCGAGTCTCCTCACACGTATGTCTCCGATGCCACATCCTCTCCCATG
  ATCACATCCCCTGGAATCTTACAGGCCTCGCCCACCCCCCTGCTGGCTGCTGCCGCCCCGGCTGCCCCAG
  TGCACACACAGCATGCGCTGTCTTTCTCTAACCTTCATGACATGCAGCCTTTGGCTCCTGGAGCCAGCAC
  CGTGCTCCCCTCGGTCAGCCAGCTGCTATCCCACCACCACATCGCGCCCCCAGGTAGTAGCAGTGCAGGA
  AGCTTGGGCAGGTTACATCCAGTTCCTGTCCCAGCAGACTGGATGAACCGTGTGGAGATGAACGAGACCC
  AGTACAGTGAAATGTTTGGCATGGTCCTGGCTCCTGCAGAGGGAGCCCACCCTGGCATAGCAGCTCCCCA
  GAGCAGACCTCCGGAAGGGAAGCACATGTCCACCCAGCGGGAGCCCTTGCCTCCCATCGTGACTTTCCAG
  CTTATCCCAAAAGGCAGCATTGCCCAGGCAGCCGGAGCTCCCCAGACGCAGTCCAGTTGCCCTCCAGCTG
  TTGCAGGCCCCTTGCCCTCTATGTACCAGATCCCAGAGATGCCCCGTTTGCCCAGTGTGGCTTTCCCACC
  TACCATGATGCCCCAGCAGGAGGGGCAGGTAGCTCAGACCATTGTGCCAACCTATCATCCTTTCCCAGCC
  TCTGTGGGCAAGTACCCCACACCCCCTTCCCAACACAGTTACGCCTCCTCAAATGCTGCTGAGCGAACCC
  CCAGTCATGGTGGTCACCTCCAGGGCGAGCACCCATACCTGACACCATCCCCAGAGTCTCCTGACCAATG
  GTCAAGCTCTTCACCACACTCTGCATCTGACTGGTCAGATGTGACCACCAGCCCAACTCCTGGAGGTGGT
  GGAGGCGGTCAGCGGGGACCCGGAACACACATGTCCGAGCCACCACACAGCAACATGCAGGTGTATGCAT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010928
• Insert Size: 7422 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010928.2, NP_035058.2
• RefSeq Size: 10506 bp
• RefSeq ORF: 7422 bp
• Locus ID: 18129
• UniProt ID: O35516
• Cytogenetics: 3 42.42 cM
• Gene Summary: Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination (PubMed:10393120). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:10393120, PubMed:18710934). Affects the implementation of differentiation, proliferation and apoptotic programs (PubMed:10393120, PubMed:18710934). May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation (By similarity). In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:18710934). Positively regulates self-renewal of liver cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]