Tpr (NM_133780) Mouse Untagged Clone

CAT#: MC225314

Tpr (untagged) - Mouse translocated promoter region (Tpr), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tpr
• Synonyms: 2610029M07Rik; C77892
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225314 representing NM_133780
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCTGGTGGCTCGGCTTCTAGAAGTGGCCACAGAGGAGTACCCATGACATCCCGGGGTTTTGACG
  GGTCCCGCCGCGGGAGTCTCAGGCGGGCAGGCGCGCGAGAGACCGCGAGCGAGGCGGCGGACGGGGCGGC
  TCCGGCGGCTGGGCTCCGCGCTTCTCCTTGCTCCTTGGCTTCTCCTTCAGCCGCTGCGGCTGTTGCCGCG
  ATCCCGGCCGACATGGCGGCGGTGTTGCAGCAAGTGCTGGAGCGCCCGGAACTGAACAAACTGCCGAAGT
  CGACGCAGAACAAACTTGAGAAGTTCCTGGCCGAGCAGCAGTCCGAGATCGACTGCCTGAAGGGGCGGCA
  CGAGAAATTTAAGGTGGAGAGTGAACAACAATACTTTGAGATAGAGAAGAGGCTCTCACAGAGTCAGGAG
  AGGCTTGTCACTGAAACCCGGGAGTGTCAGAACTTGAGGCTGGAGCTTGAGAAGCTAAATAACCAAGTAA
  AAGTGTTAACTGAAAAAACCAAAGAACTGGAAACTGCTCAAGACCGTAATCTGGGCATTCAGAGCCAGTT
  TACAAGAGCAAAGGAAGAGTTAGAAGCTGAAAAAAGAGATTTAATCAGAACCAATGAGAGGTTATCTCAG
  GAAGTTGAATATTTAACAGAGGATGTTAAACGTCTAAATGAAAAACTTAAAGAAAGCAATACGACGAAGG
  GTGAACTTCAGTTAAAGCTGGATGAACTTCAAGCTTCTGATGTTGCTGTGAAGTATCGAGAAAAACGCTT
  AGAACAAGAAAAGGAATTGCTACACAATCAAAATTCATGGCTGAACACAGAGTTGAAAACCAAAACTGAT
  GAGCTGTTGGCTCTAGGAAGAGAAAAAGGAAATGAAATTCTGGAACTTAAGTGTAATCTTGAAAACAAAA
  AAGAAGAGGTTCTGAGACTGGAAGAACAGATGAATGGCTTGAAAACATCAAATGAACATCTTCAGAAACA
  TGTAGAGGATTTGTTGACAAAATTAAAAGAGGCCAAAGAACAGCAGGCCAGTATGGAAGAGAAATTCCAC
  AATGAGTTAAATGCTCACATAAAGCTCTCTAATCTCTACAAGAGCGCTGCTGATGACTCAGAGGCAAAGA
  GTAATGAGCTAACCCGGGCAGTGGACGAGCTGCACAAACTGCTCAAAGAAGCAGGAGAAGCCAACAAAAC
  AATACAAGATCATCTTCTACAAGTGGAAGAATCTAAAGATCAGATGGAAAAAGAAATGCTTGAGAAAATA
  GGAAAATTGGAGAAGGAATTAGAGAATGCAAATGACCTGCTTTCTGCTACAAAACGTAAAGGAGCTATAT
  TGTCTGAAGAAGAGCTTGCAGCCATGTCTCCCACCGCAGCAGCTGTAGCTAAAATTGTAAAGCCTGGCAT
  GAAATTAACTGAGCTATATAATGCTTATGTGGAAACTCAGGATCAGTTGCTTTTGGAAAAACAAGAAAAT
  AAAAGGATTAATAAGTATCTGGATGAGATAGTAAAAGAAGTGGAAGCCAAAGCACCGATTTTGAAACGCC
  AGCGCGAAGAGTATGAGCGTGCACAGAAAGCTGTGGCCAGTCTTTCTGCAAAGCTTGAGCAAGCTATGAA
  GGAGATTCAGCGATTGCAGGAGGACACTGATAAAGCCAACAAACATTCATCGGTACTTGAAAGGGACAAT
  CAGAGAATGGAAATACAAATAAAAGACCTTTCGCAACAGATAAGAGTGCTCTTGATGGAACTTGAAGAAG
  CCAGGGGTAACCATGTAATTCGGGATGAGGAGGTCAGCTCTGCTGACATCAGTAGTTCGTCTGAAGTGAT
  TTCGCAGCACCTAGTATCTTACAGGAACATCGAAGAGCTTCAGCAGCAGAATCAGCGTCTGCTATTCGCT
  CTTCGAGAACTTGGGGAAACCAGGGAACGAGAAGAGCAAGAGACAACTTCCTCTAAGATTGCTGAACTTC
  AGCATAAACTTGAGAACTCTCTCGCTGAACTGGAACAGCTCCGTGAGTCACGACAACATCAGATGCAGCT
  TGTTGATTCCATAGTGCGTCAGCGTGACATGTACCGAATTTTATTGTCACAAACAACAGGAATGGCCATT
  CCCTTACAAGCTTCAAGTTTAGATGATATTTCTCTTCTCTCAACTCCAAAACGTTCAAGTACATCACAGA
  CTGTTTCCACTCCTGCTCCGGAACCCGTCATTGACTCCACAGAGGCTATAGAAGCAAAGGCTGCCCTTAA
  ACAGTTGCAGGAAATCTTTGAGAACTATAAAAAAGAAAAAATAGACAGTGAGAAACTTCAAAATGAGCAG
  CTAGAGAAACTTCAAGAGCAAGTCACAGACCTGCGCTCACAAAACACCAAAATTTCTACTCAGCTAGATT
  TTGCTTCTAAACGTTATGAAATGCTGCAAGATAATGTAGAAGGATATCGTCGAGAAATAACATCTCTACA
  AGAGAGAAATCAGAAACTCACTGCAACTACTCAGAAGCAGGAACAGATCATCAACACAATGACGCAAGAC
  TTGAGAGGAGCCAATGAAAAGCTAGCAGTTGCAGAAGTAAGAGCAGAAAATTTAAAGAAGGAAAAAGAAA
  TGCTTAAATTGTCTGAAGTCCGTCTTTCTCAGCAAAGAGAGTCTTTGTTAGCTGAACAGAGGGGGCAGAA
  CTTGTTGCTGACTAATCTACAGACAATCCAGGGCATACTAGAGCGATCTGAGACTGAAACCAAACAAAGG
  CTGAATAGCCAGATAGAAAAACTGGAGCATGAGATCTCTCACTTGAAGAAGAAATTGGAAAACGAGGTGG
  AACAAAGACATACGCTTACGAGGAATCTAGACGTTCAACTATTAGATACCAAGAGACAGCTAGATACAGA
  AATAAATCTCCATCTTAACACAAAAGAACTGTTAAAAAATGCTCAGAAGGACATTGCTACACTGAAACAG
  CACCTTAATAATATGGAAGCCCAGCTCGCTTCACAGTCCACACAGAGAACCGGTAAAGGTCAGCCTGGTG
  ACAGAGATGACGTTGATGATCTTAAAAGTCAGCTAAGGCAGGCTGAAGAACAGGTCAATGACTTGAAGGA
  GAGACTCAAGACAAGTACCAGTAATGTGGAGCAGTATCGAGCCATGGTGACCAGCTTAGAAGATTCCCTG
  AACAAGGAGAAACAGGTGACTGAGGAGGTTCATAAGAACATTGAAGTTCGCTTGAAAGAATCAGCAGAGT
  TTCAGACACAGTTGGAGAAGAAATTGATGGAAGTAGAGAAGGAAAAGCAAGAACTTCAGGATGATAAGAG
  GAAAGCCATCGAGAGCATGGAACAGCAATTAAGTGAATTGAAGAAAACACTGTCTACTGTTCAGAATGAA
  GTACAAGAAGCTCTTCAGAGAGCAAGCACAGCTTTAAGTAATGAACAGCAAGCCAGGCGTGACTGTCAGG
  AACAAGCTAAAATCGCTGTGGAAGCCCAGAATAAGTATGAGAGAGAATTGATGCTACATGCTGCTGATGT
  CGAAGCTCTGCAAGCTGCCAAGGAACAGGTTTCAAAAATGACATCAATCCGTCAGCATTTGGAAGAAACA
  ACTCAGAAAGCAGAATCCCAGTTGTTGGAATGTAAAGCATCTTGGGAAGAGAGAGAGAGAGTGTTGAAGG
  ATGAAGTTTCCAAAAGTGTGTCTCGCTGTGAAGATCTAGAGAAACAAAACCGACTACTTCATGATCAAAT
  CGAAAAATTAAGTGACAAGGTGGTCACCTCTATGAAGGATGCTGTGCAAGCGCCATTAAATGTATCTCTC
  AATGAAGAAGGGAAATCTCAAGAACAAATTCTAGAAATTCTTAGATTTATTCGACGAGAAAAAGAAATTG
  CTGAAACTAGGTTTGAGGTGGCTCAGGTTGAGAGTTTGCGGTATCGACAAAGAGTTGAACTTCTAGAACG
  GGAGTTGCAAGAACTGCAGGATAGCCTGAATGTGGAAAGGGAAAAGGTGCAGGTAACTGCAAAAACAATG
  GCGCAACATGAAGAGCTGATGAAGAAAACTGAAACAATGAACGTTGTTATGGAGACCAACAAGATGCTAC
  GGGAGGAAAAGGAAAGGCTCGAACAGAACCTACAACAAATGCAAGCCAAGGTGAGGAAACTGGAGTTAGA
  CATTTTACCCTTACAAGAAGCCAATGCTGAGCTGAGTGAGAAAAGCGGTATGCTCCAGGCAGAAAAGAAG
  CTACTGGAAGAGGATGTTAAGCGTTGGAAAGCACGGAACCAGCAACTAATAAATCAACAGAAAGATCCAG
  ACACAGAAGAATATCGAAAGTTGCTTTCTGAAAAGGAAATTCACACTAAGAGAATCCAACAGCTAAATGA
  AGAAGTTGGAAGGCTTAAGGCTGAAATTGCAAGATCAAATGCATCCTTGACTAACAACCAGAATTTAATC
  CAGAGTCTGCGGGAAGACTTAAGTAAAGCACGGACTGAAAAGGAAGGCATTCAGAAAGATTTAGATGCCA
  AAATAATTGATATACAGGAAAAGGTCAAAACAATCACTCAAGTCAAGAAAATTGGACGCAGGTACAAGAC
  TCAGTTTGAAGAACTGAAAGCGCAACAAAACAAGGCCATGGAGACTTCCACTCAGTCTTCAGGAGACCAT
  CAGGAACAGCACATCTCTGTCCAGGAAATGCAGGAGCTCAAAGATACCCTCAGCCAATCCGAAACAAAGA
  CAAAGTCACTTGAGGGTCAAGTAGAGAATCTGCAGAAGACATTATCTGAAAAAGAGACAGAAGCAAGAAG
  TCTCCAGGAGCAGACGGTGCAGCTTCAGTCTGAACTGTCACGCCTTCGTCAGGACCTCCAGGATAAAACC
  ACAGAGGAGCAGCTCCGACAGCAAATGAATGAAAAGACTTGGAAGACACTTGCACTAGCCAAATCAAAAA
  TTACACATCTATCTGGTGTGAAAGATCAGCTGACTAAAGAAATTGAAGAACTTAAACAAAGGAACGGAGC
  TCTAGATCAGCAGAAAGATGAGCTTGATGTCCGAATGACTGCTCTTAAGTCCCAGTATGAAGGTCGGATT
  AGTCGCTTAGAGAGAGAGCTCAGAGAGCATCAAGAGCGACACCTTGAGCAGAGGGATGAGCCTCAAGAAC
  CCACTAATAAGGCTCCAGAACAGCAAAGACAAATCACACTGAAAACCACTCCAGCTTCTGGTGAAAGAGG
  AATTGCCAGCACATCAGACCCACCAACAGCCAATATCAAGCCAACTCCTGTTGTTTCTACTCCAAGTAAA
  GTGACAGCTGCAGCTATGGCTGGGAATAAGTCGACACCCAGGGCAAGTATCCGCCCAATGGTTACACCTG
  CAACGGTCACAAATCCGACCACTACCCCAACAGCAACCGTAATGCCTACTACACAGGTGGAGTCACAGGA
  AGCTATGCAGTCAGAAGGGCCTGTGGAACATGTTCCAGTATTTGGAAACGCAAGTGGATCTGTTCGTTCT
  ACTAGTCCTAATGTTCAGCCCTCCATTTCTCAGCCCATCCTAACTGTACAGCAGCAAACACAAGCTACAG
  CTTTTGTACAACCCACTCAACAGAGCCACCCGCAGATTGAGCCTACAAATCAAGAATTGTCCCCAAACAT
  AGTTGAGGTAGTACAAAGTTCCCCAGTGGAGCGTCCATCTACTTCCACAGCAGTATTTGGCACTGTTTCA
  GCTACCCCAAGTTCCTCCTTGCCAAAGCGCACTCGGGAGGAGGAAGAGGACAGCACCATGGAAGCAGGAG
  ACCAAGTCTCTGAGGACACAGTGGAAATGCCTCTCCCGAAGAAGCTGAAGATGGTCACACCCGTTGGAAC
  CGAGGAAGAAGTTATGGCAGAAGAAAGTACTGATGGAGAGGCAGAAACTCAAGCCTATAATCAGGACTCT
  CAAGATTCCATTGGAGAAGGAGTTACCCAGGGAGATTATACACCAATGGAAGACAGTGAAGAAACATCAC
  AATCTCTACAAATCGATCTTGGACCACTTCAGTCAGATCAACAGACTACTTCCTCTCAAGATGGTCAAGG
  CAAAGGAGATGATGTAATTGTAATTGACAGTGATGATGAAGATGATGATGAAGAAAATGATGGCGAACAT
  GAAGATTATGAAGAAGACGAAGATGATGATGATGATGAAGAAGATGACACGGGGATGGGAGATGAGGGTG
  AAGATAGTAATGAAGGAACTGGTAGTGCAGATGGTAATGATGGATATGAAGCTGATGATGCTGAGGGTGG
  TGATGGGACTGATCCAGGTACAGAAACAGAAGAAAGTATGGGTGGAGCTGAAAGTCATCAGAGAGCTGCT
  GATTCTCAAAACAGTGGTGAAGGAAATACAAGTGCAGCAGAGTCTTCCTTCTCTCAGGAGGTTGCCAGGG
  AACAGCAGCCCACGTCAGCGTCTGAGAGACAGACTCCGCAAGCACCGCAGTCACCAAGGCGCCCTCCACA
  TCCTCTCCCCCCACGCCTGACCATTCACGCCCCACCTCAGGAGCTGGGACCACCAGTTCAGAGAATTCAG
  ATGACCCGGAGGCAGTCTGTAGGGCGTGGTCTTCAGTTGACTCCAGGAATAGGTGGCATGCAACAACACT
  TTTTTGATGATGAAGACAGAACAGTCCCGAGTACCCCAACTCTTGTGGTGCCACATCGCACTGATGGGTT
  TGCTGAAGCTATTCATTCCCCACAGGTTGCCGGTGTTCCTAGATTCCGGTTTGGGCCACCTGAAGACATG
  CCACAGACAAGTTCCAGTCACTCTGATCTTGGCCAACTTGCTTCTCAAGGAGGTTTGGGAATGTATGAAA
  CACCCCTTTTTCTGGCTCATGAAGAAGAGTCTGGTGGCCGCAGCGTTCCCACTACGCCTCTCCAAGTAGC
  AGCCCCAGTGACTGTGTTCACTGAGAGTACCACCTCTGATGCTTCAGAACATGCCTCTCAGTCGGTTCCC
  ATGGTGACAACGTCTACGGGCACTTTATCCACGACAAACGAAACAGCAGCAGGTGACGACGGAGATGAAG
  TATTTGTAGAGGCGGAGTCTGAAGGTATTAGTTCAGAGGCAGGCCTAGAAATTGATAGCCAGCAGGAAGA
  GGAGCCTGTCCAAGCATCTGATGAATCAGATCTTCCCTCAACCAGCCAAGATCCCCCTTCGAGCTCCTCC
  GTAGATACCAGTAGTAGTCAACCGAAGCCTTTCAGACGAGTAAGACTTCAGACAACCTTGAGACAGGGTG
  TTCGTGGTCGTCAGTTTAACCGACAAAGAGGTATAAGCCATGCAATGGGAGGAAGAGGAGGAATAAATAG
  AGGGAATATTAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133780
• Insert Size: 7296 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133780.3, NP_598541.3
• RefSeq Size: 7550 bp
• RefSeq ORF: 7296 bp
• Locus ID: 108989
• UniProt ID: F6ZDS4
• Cytogenetics: 1 G1
• Gene Summary: Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Plays a limited role in the regulation of nuclear protein export. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. Plays also a role as a structural and functional element of the perinuclear chromatin distribution; involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases (By similarity).[UniProtKB/Swiss-Prot Function]