Cep250 (NM_177217) Mouse Untagged Clone

CAT#: MC225304

Cep250 (untagged) - Mouse centrosomal protein 250 (Cep250), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cep250
• Synonyms: AW490617; B230210E21Rik; Cep2; Inmp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225304 representing NM_177217
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACAGGGAGCCCCGGGTTGAACATGAAGCCCCAGTCCTTGCAGCTGGTGCTGGAGGGGCAGGTGC
  TGGCGCTGCAGCAGCAGATGGCCGAGAATCAGGCTGCCTCCTGGCGGAAACTGAAGAACTCCCAAGAGGC
  CCAGAAGAGACAAGCAACCCTCGTGAGGAAGCTGCAGGCCAAGGTTTTACAGTACCGGAGCTGGTGTCAA
  GACCTGGAGAAGCGACTAGAAGCCACTGGGGGGCTGATCCCTCAGCGCTGGGAGAGTGTAGAAGAGCCCA
  ACCTGGAACAGTTGCTGATCCGATTGGAGGAGGAGCAGCAGAGGTGTGAGAGTCTAGTGGAGGTGAACAC
  CGAGCTTCGGCTGCACATGGAGAAAGCCGATGTGGTGAATAAAGCCTTGCAGGAGGACGTGGAAAAACTG
  ACAGTGGACTGGAGTCGGGCCCGAGATGAGTTAGTGAGGAAGGAGAGCCAGTGGCGGATGGAGCAGGAGT
  TCTTCAAGGGCTATTTGAGAGGTGAACACGGTCGTCTTCTCAATCTATGGCGAGAGGTGGTAACCTTCCG
  ACGTCACTTCCTGAAAATGAAGTCAGCTACTGACAGAGACCTGACAGAGCTAAAGGCTGAACACGCAAGG
  CTTTCAGGGTCCCTGCTGACCTGCTGTCTGCGCCTGACCCTGAGAGCACAGTCTCGGGAGTCCAGTGGAT
  CTGGGAGAACAGAGGAGAGTGAGCCAGCCCGGCTGCTGCTGCTCGTCGCTAAGACCCAGGCGCTGGAGAA
  GGAAGCCCATGAGAAGAGCCAGGAGTTAATGCAGCTCAAGAGTCACGGGGATCTAGAGAAGGCAGAGCTG
  CAGGACCGAGTGACGGAACTCTCTGCTCTGCTAACCCAGTCTCAGAAGCAAAATGAAGATTATGAGAAGA
  TGGTAAAGGCTCTAAGAGAAACAATGGAGATCCTGGAGACAAACCATGCAGAGTTAATGGAGCATGAGGC
  ATCTCTCAGTAGGAATGCTCAAGAGGAAAAGTTGTCTCTGCAGCAGGTGATCAAGGCTATAACCCAGGCA
  CTGGCCTCAGTGGAAGAAGAAGACACTGTGACCCAGTCCTCAGGCCATGAGGACTCGCTGCAGTCAGACT
  GTAATGGCCTTTCCCAGTTTGATCCCCAGGACCCAGACAGGGCTCTTACCCTGGTGCAGTCAGTGCTGAC
  TCGGAGACAGCAGGCTGTGCAGGACCTCAGGCAGCAGCTTTCAGGCTGCCAGGAGGCTATGAGCTTCTTG
  CAGCAGCAGCACGATCAGTGGGAGGAAGAGGGCAGGGCCCTGAGGGAAAAGCTACAGAAGCTCACTGGGG
  AGCGGGATGCTCTGGCAGGCCAGACTGTGGGCCTTCAGGGAGAGGTGGACTCTCTCAGCAGGGAGCGGGA
  GCTGCTTCAGAAGGCCAGAGGAGAGCTGCAGCAGCAACTGGAAGTGCTGGAGCAGGAAGCGTGGCGACTG
  CGGAGGATGAACATGGAGCTGCAGCTGCAGGGGGACTCAGCTCAAGGGGAGAAGCTGGAGCAGCAGGAGG
  AGTTGCACTTGGCTGTCCGGGAGAGGGAGCGCCTTCAGGAGACGCTGGTGGGCCTGGAAGCCAAGCAGTC
  AGAATCACTAAGTGAGCTGCTCACTCTTCGAGAAGCCCTGGAGTCAAGTCGCCTAGAAGGGGAGTTACTG
  AAGCAGGAGCGAGTGGAAGTGGCTGCAGCCTTGGCCAGGGCAGAACAGTCCATTGTGGAGCTGTCAGGTT
  CTGAGAACAGCCTGAAGGCAGAGGTAGCTGATCTTCGAGCTGCAGCTGTCAAGCTTGGGGCCTTAAACGA
  GGCTTTGGCTTTAGATAAAGTTGAACTGAACCAGCAGCTTCTCCAGTTGGAGCAGGAGAACCAGTCTCTA
  TGCAGCAGAGTGGAGGCCGCAGAGCAGCTGAGAAGCGCTTTGCAGGTGGACCTGGCGGAGGCAGAAAGGC
  GCAGGGAAGCCCTGTGGGAGAAGAAAACTCAGCTGGAGACTCAGCTGCAGAAGGCAGAGGAAGCGGGGGC
  TGAGCTACAGGCAGAGCTGAGGGGCACCCGAGAAGAAAAGGAAGAGCTTAAAGATAAATTAAGTGAGGCA
  CATCACCAGCAAGAAACAGCTACAGCTCACCTAGAACAGCTGCATCAGGACGCAGAAAGACAGGAAGAAA
  CACTTGCTAGGGCAGTCCAGGAGAAGGAAGCCCTAGTACGGGAGAGGGCAGCTCTTGAGGTGCGCCTGCA
  GGCTGTGGAGCGGGACCGACAGGACCTCACTGAACATGTCCTGGGGCTCAGGTCAGCCAAGGAGCAACTG
  GAGAGCAATCTATTTGAGGCCCAACAGCAAAATTCTGTGATACAGGTCACCAAGGGGCAGCTGGAGGTCC
  AGATTCAAACTATTATCCAAGCCAAGGAAGTAATTCAAGGGGAAGTGAAATGCCTGAAGCTGGAACTGGA
  TGCTGAGAGGACCCGGGCAGAGCAGGAGTGGGATGCAGTAGCCAGGCAGCTAGCCCAAGCTGAGCAGGAG
  GGGCAAGCATCTCTGGAGCGACAGAAGGTGGCCCACGAGGAGGAGGTGAACAGGCTGCAAGAGAAATGGG
  AGAAAGAGCGCTCCTGGCTGCAGCAGGAGCTGGATAAGACCCTGGAAACCCTCGAGAGGGAGAGAGCAGA
  GCTGGAGACAAAGCTGAGAGAGCAGCAGACAGAAATGGAAGCCATCCGTGCACAGAGGGAAGAGGAACGA
  AGCCAGGCTGACAGCGCCCTGTATCAAATGCAGCTAGAAACAGAGAAGGAGAGAGTATCCCTCCTGGAGA
  CACTGCTGAGGACCCAGAAGGAGCTGGCAGATGCTAGTCAACAGCTGGAAAGGCTGAGACAGGACATGAA
  GATTCAGAAGTTAAAGGAACAGGAGACCACTGGCATGCTGCAGGCCCAGCTTCAGGAAACACAGCAGGAG
  CTGAAGGAGGCAGCCCAGCAGCACAGGGACGACCTTGCTGCCTTCCAGAAGGACAAGCTGGACCTGCAGA
  AGCAGGTAGAGGACCTAATGTCTCAGCTGGTAGCCCACGATGACTCCCAGAGGCTGGTGAAAGAAGAGAT
  TGAAGAGAAGGTGAAAGTGGCTCAAGAATGCAGCCGGATTCAGAAGGAACTGGAGAAAGAGAACGCCAGC
  CTGGCTCTGTCGCTGGTGGAAAAGGAAAAACGACTTCTCATTTTACAAGAAGCTGACTCTGTAAGACAGC
  AGGAGCTGAGCTCCTTGCGCCAGGACATACAGGAGGCCCAGGAAGGGCAAAGAGAGCTGGGTGTACAGGT
  GGAACTATTGAGGCAGGAGGTGAAGGAAAAGGAGGCTGACTTTGTGGCCCGGGAAGCACAGCTGCTGGAG
  GAGTTGGAGGCCTCACGGGTTGCAGAGCAGCAACTTCGAGCGTCTTTGTGGGCCCAGGAAGCCAAGGCAA
  CCCAGCTGCAGCTGCAGCTGCGCAGCACTGAGAGCCAGCTAGAGGCATTGGTTGCAGAACAACAGCCGGA
  GAACCAGGCACAGGCACAGCTAGCTAGCCTCTGCTCTGTTCTGCAGCAGGCCCTGGGGTCTGCATGTGAG
  AGTAGGCCTGAGCTGAGGGGTGGAGGCGACTCTGCTCCTACCTTATGGGGCCCTGATCCAGATCAGAATG
  GAGCTTCCAGACTCTTTAAGAGATGGTCGCTCCCAACGGCACTGTCACCGGAGGCTGTGGCACTAGCTCT
  CCAGAAGCTCCATCAGGACGTGTGGAAGGCTCGGCAGGCCCGGGATGACCTAAGGGACCAGGTCCAGAAG
  CTGGTGCAGCGCCTGACTGACACGGAGGCCCAGAAGAGCCAGGTTCACTCGGAGCTGCAGGACCTGCAGA
  GACAGCTCTCTCAGAGCCAGGAAGAGAAATCCAAGTGGGAAGGGAGGCAGAACTCCCTGGAGTCTGAGCT
  GAGGGACTTGCACGAGACCGCGGCATCCTTACAGAGTCGCCTCCGGCAGGCAGAACTGCAAAAAATGGAA
  GCTCAGAATGATCGAGAGTTGCTTCAGGCATCCAAGGAGAAGCTGTCAGCCCAGGTTGAACATCTGCAAG
  CATGTGTTGCAGAAGCCCAGGCTCAGGCAGATGCAGCTGCTGTCCTGGAAGAAGATCTGCGAACTGCTCG
  CTCTGCACTGAAACTGAAAAACGAGGAGCTAGAGAGTGAACGGGAGAGAGCCCAGGCTTTACAAGAACAG
  GGTGAGCTGAAGGTGGCTCAGGGGAAAGCTTTGCAGGAGAACCTAGCCCTGCTGGCACAGACCCTGTCTA
  ACAGAGAGCGGGAGGTCGAGACGTTGCAGGCTGAGGTTCAGGAACTGGAGAAGCAGCGGGAGATGCAGAA
  GGCTGCTCTGGAGCTGCTCTCCCTCGACCTGAAGAAGCGCAGTCGGGAGGTAGACCTGCAACAAGAACAG
  ATTCAGGAGCTGGAGCAGTGCAGGTCAGTCTTAGAGCACTTGCCCATGGCCGTCCAAGAGCGAGAGCAGA
  AACTGAGTGTGCAGCGGGACCAGATCAGGGAACTGGAGAACGATCGGGAGGCCCAGAGGAGTGTCCTGGA
  ACACCAGCTTCTGGACCTGGAGCAGAAGGCTCAAGTGATTGAGTCCCAGAGGGGACAGATTCAGGATCTG
  AAAAAGCAACTGGGGACACTGGAATGCCTGGCCCTGGAACTGGAGGAAAGCCATCACAAAGTGGAGAGCC
  AACAGAAGATGATCACAGAACTAGAGGGCCAGAGGGAAATGCAGAGGGTGGCTCTAACCCACCTCACACT
  GGACCTGGAAGAAAGGAGCCAGGAGCTGCAGGCACAAAGCAGCCAGCTCCATGAGCTGGAGAACCATAGC
  ACCCATCTGGCAAAAGAGCTGCAGGAGAGGGACCAGGAGGTGACGTCCCAACGCCAGCAGATCGATGAGC
  TGCAGAAGCAGCAGGAACAGCTGGCTCAGGCCCTGGAGAGGAAAGGCCAGGAGCTGGTGCTGCAGAAGGA
  AAGGATTCAGGTTCTGGAAGATCAGAGGACGCTGCAGACCAAGATCTTAGAGGAGGACCTAGAACAGATC
  AAGCATTCCTTGAGAGAACGCAGCCAGGAGCTGGCCTCTCAGTGGCAGCTGGTGCATGAGCGTGCCGACG
  ATGGAAAGAGCCCGAGTAAAGGCCAGCGAGGGAGCCTGGAGCACTTGAAGCTGATCCTGCGTGATAAGGA
  GAAGGAGGTAGAGTGCCAGCAGGAGCGCATCCAGGAACTTCAGGGCCACATGGGCCAGCTGGAACAGCAG
  CTGCAGGGTCTGCACAGGAAGGTGGGGGAGACTAGCCTGCTCCTGACCCATCGAGAGCAGGAGACAGCAA
  CCCTGCAGCAGCATCTCCAGGAAGCCAAGGAACAAGGGGAACTGAGAGAGCAGGTACTTCAGGGTCAGCT
  GGAGGAGGCCCAGAGAGATCTGGCCCAGAGGGACCATGAACTAGAGACCCTCCGGCAGGAAAAACAGCAG
  ACCCAGGACCAGGAGGAAAGCATGAAGCTAAAGACAAGTGCTCTGCAGGCCGCTCTGGAGCAGGCCCATG
  CGACACTGAAGGAGCGTCAGGGGGAGCTGGAAGAACACAGGGAACAGGTGCGAAGGCTCCAGGAAGAACT
  AGAAGTGGAGGGACGGCAGGTACGGGCCCTGGAGGAGGTGTTGGGGGACCTAAGGGCTGAGTCTCGGGAG
  CACGAGAAGGCTGTGCTGGCCCTTCAACAACGGTGTGCTGAGCAGGCCCAGGAACATGAGGCAGAAGCCA
  GGACCCTGCAGGACAGCTGGCTGCAGGCCCAGGCCACTCTCACAGAACAAGAACAAGAGCTAGCGGCACT
  GCGAGCGGAAAACCAGTATTCCCGCCGACAGGAAGAGGCTGCAGTTAGCCAGGCAGAGGCGCTGCAGGAG
  GCCCTCAGCAAAGCCCAGGCTGCCCTGCAGGAGAAAGAGCAGAGTCTGCTTGAACAGGCTGAGTTGAGCC
  ACACCCTGGAGGCCAGCACTGCCGCCTTGCAAGCTACCCTGGACACCTGCCAGGCAAGTGCCCGGCAGCT
  GGAGGAGGCCCTGAGGATTCGGGAAGGTGAGATCCAGGCCCAGGCTCTCCAACACCACGAGGTCACGCAG
  CACCTCCAGCAGGAACTTTGCCAGAAGAAAGAAGAGCTGAGGCAGCTACTAGAGAAGGCTGGGGCCCGGA
  GGAGTCAAGAAAATGGAATCCAAGAGAAGCAAAGTCTGGAGCAAGAGAGACAAGAGGAAACAAGGAGGCT
  CCTTGAGAGCCTAAAGGAGCTACAGCTGACTGTAGCCCAAAGGGAAGAAGAGATTCTGATGCTGAGGGAG
  GCCTCGTCCCCACGCCACAGAGCCCTCCCAGCCGAGAAGCCAGCTCTGCAACCGCTCCCAGCACAACAGG
  AGTTAGAGCGGCTGCAGACTGCCCTAAGGCAAACAGAGGCCAGGGAGATTGAATGGAGGGAGAAGGCTCA
  GGACCTGGCTCTGTCCCTGGCCCAAAGCAAGGCCAGCATCAGCAGTCTGCAGGAGATAACCATGTTCCTA
  CAAGCCTCTGTTCTAGAGCGGGAATCAGAACAACAAAGACTACAGGAGGAGTTGGTACTCAGCAGACAAG
  CTCTGGAAGAGCAGCAGTCAGGTGGCCCACACTCAACCAGCAGAGCAGACCAGGGACCAAAAGTGGGACA
  GGGAAGCCAGTCTGGAGAGGTAGAGACTGAGCCTAGTCCTGGAGTGGAGGAGAAGGAACGGTTGACACAG
  AGGCTTGAACGTCTGCAGCAAGCGGTGGCAGAGCTGGAAGTTGACCGAAGCAAGCTGCAGTGCCACAATG
  CGCAGCTGCGGACCGCCTTGGAGCAGGTGGAACGAGAACGGAGGAAGCTGAAGAGGGATTCCGTGCGTGC
  ATCCCGGGCAGGATCACTGGAGGCCAGAGAAACTATGACTTCATCCCCCACACAGCAGGATGGGAGAGGC
  AGTCAGAGGGGTTCCTCGGACAGCGTGCTTGTGGTTGAGCTTCAGAGAGAGGTAGCCCTGCTGCGAGCCC
  AGCTGGCCTTGGAGCGAAAGCAGAGGCAGGACTACATTGCACGGTCCGTGCAGACCAGCCGTGAGCTAGC
  AGGCCTGCATCACAGCCTGTCTCACTCCCTCCTCACCGTGGCCCAGGCCCCTGAGGCCACTGTTCTGGAG
  GCTGAGACCCGAAAGCTGGATGAGTCTCTGAATCAGAGTCTGACATCCCCAGGGCCATGTTTGCTACATC
  CCAGTCTGGACACTACTCAAAATACCCACAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177217
• Insert Size: 7245 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177217.3, NP_796191.2
• RefSeq Size: 7914 bp
• RefSeq ORF: 7245 bp
• Locus ID: 16328
• UniProt ID: Q60952
• Cytogenetics: 2 H1
• Gene Summary: May be involved in ciliogenesis. Probably plays an important role in centrosome cohesion during interphase.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.