Cacna1b (NM_007579) Mouse Untagged Clone

CAT#: MC225258

Cacna1b (untagged) - Mouse calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacna1b
• Synonyms: alpha(1B); AW050276; AW060892; AW822256; BIII; Cav2.2; Cchn1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225258 representing NM_007579
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGTG
  GCGGGGCCGGCGGGGCGGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA
  GTCCATTGCGCAGCGCGCACGGACTATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC
  GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCTAAGCGCATCACCGAAT
  GGCCGCCCTTCGAATACATGATCCTGGCCACCATCATCGCCAACTGCATTGTTCTGGCCCTGGAGCAGCA
  CCTCCCTGATGGGGACAAGACTCCCATGTCTGAGCGACTAGATGACACGGAGCCTTACTTCATCGGGATC
  TTTTGCTTCGAGGCGGGCATCAAGATCATAGCCCTGGGCTTTGTTTTCCACAAGGGCTCCTACCTTCGGA
  ACGGCTGGAATGTCATGGACTTCGTGGTGGTCCTCACGGGGATTCTCGCCACAGCTGGAACTGACTTTGA
  CCTGCGCACACTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGCTGGTGTCTGGAATTCCAAGCTTGCAG
  GTGGTGCTTAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATTGGGCTGCTGCTCTTCTTTGCCA
  TCCTCATGTTTGCCATCATCGGCCTCGAATTCTATATGGGCAAATTCCATAAGGCCTGTTTCCCCAACAG
  CACAGATACAGAGCCTGTGGGTGACTTTCCCTGTGGCAAAGATCCCCCTGCTCGTCAGTGTGATGGTGAC
  ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA
  TCTTGACAGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC
  TGGCAACACATGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG
  GTGCTGGGTGTGCTTTCCGGAGAGTTTGCCAAGGAGCGGGAGCGAGTCGAGAACCGCCGCGCCTTCCTGA
  AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAGCTGAATGGGTACTTGGAGTGGATCTTCAAGGCAGAGGA
  AGTCATGTTGGCAGAGGAGGACAAGAATGCAGAAGAGAAATCCCCTTTGGATGTGTTGAAGAGAGCTGCC
  ACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTGCAG
  TTGGGTCTCCATTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACGGAGAGCTCATCGTACTTCCGGAGAAA
  GGAGAAGATGTTCCGGTTCTTTATCCGGCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTGTGT
  GTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCACTATAATCAGCCTCAGCGGCTTACCACTGCAC
  TGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTATGGCCTAGG
  GCCCAGAAGTTACTTCAGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTTGAA
  GTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTTGGAATCAGTGTGCTGCGGGCTCTGCGACTGCTGA
  GGATATTCAAAGTTACCAAGTATTGGAACTCTCTGAGGAACCTGGTGGTTTCCCTCCTCAATTCCATGAA
  GTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTCTTCATCGTGGTCTTCGCTCTGTTGGGGATGCAGCTG
  TTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCATCC
  TCACTGTCTTTCAGATCCTGACAGGAGAGGATTGGAATGCCGTAATGTATCATGGGATTGAGTCGCAAGG
  TGGAGTCAGCAAAGGCATGTTTTCTTCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACCCTG
  CTGAATGTTTTTCTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAGAGG
  AGATGGAAGAAGCAGCCAATCAGAAACTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCCCAT
  GTCTGCTGCCAATATCTCCATCGCTGCGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAGCGG
  GCCAGTCAGCTAAGGCTCCAGAATCTGCGTGCCAGCTGTGAGGCATTGTACAGTGAGATGGACCCTGAGG
  AGCGCCTGCGTTATGCCAGCACGCGCCATGTGAGGCCAGACATGAAGACACACATGGACCGACCCCTAGT
  GGTGGAGCCTGGTCGAGATGGCTTGCGGGGACCCGTTGGGAGCAAGTCAAAGCCTGAAGGCACGGAGGCC
  ACAGAAAGCGCGGACCTACCTCGCAGGCACCACCGGCACCGTGATAGGGACAAGACCTCAGCCACAGCAC
  CTGCTGGAGGCGAACAGGACAGGACAGAAAGCACCGAGACCGGGGCCCGGGAGGAACGTGCGCGCCCTCG
  TCGAAGTCACAGCAAGGAGACTCCAGGGGCTGACACGCAAGTGCGCTGTGAGCGCAGTAGACGTCACCAC
  CGGCGCGGCTCCCCGGAGGAGGCCACTGAACGGGAGCCTCGGCGCCACCGTGCCCACCGGCATGCACAGG
  ACTCAAGCAAGGAGGGCACGGCGCCGGTGCTTGTACCCAAGGGTGAGCGACGAGCAAGACACCGAGGCCC
  ACGCACGGGTCCACGTGAGGCAGAGAACAACGAGGAGCCCACACGCAGGCACCGTGCAAGGCATAAGGTG
  CCACCCACACTGCAGCCCCCAGAGAGGGAGGCTGCAGAGAAGGAGAGCAACGCGGTGGAAGGGGATAAGG
  AAACCCGAAATCACCAGCCCAAGGAACCTCACTGTGACCTGGAGGCCATTGCAGTTACAGGTGTGGGCCC
  TCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAGCAACCAGAGGATGCAGACAACCAGCGT
  AATGTCACCCGGATGGGCAGTCAGCCCTCAGATCCCAGCACCACTGTGCATGTCCCAGTGACACTGACAG
  GCCCTCCTGGGGAGACCCCTGTAGTTCCCAGTGGTAACATGAACCTGGAAGGCCAAGCAGAGGGCAAGAA
  GGAGGCAGAGGCGGATGATGTGCTGAGAAGAGGCCCCAGGCCCATCGTTCCCTACAGCTCCATGTTTTGT
  CTCAGCCCCACCAACCTGCTTCGTCGCTTCTGCCATTACATTGTGACCATGCGGTACTTTGAGATGGTAA
  TTCTTGTGGTCATTGCCTTGAGCAGCATTGCCCTGGCTGCAGAGGATCCTGTGCGGACAGATTCATTCAG
  GAACAACGCTTTAAAGTACATGGATTACATCTTTACAGGAGTCTTCACCTTTGAAATGGTCATAAAGATG
  ATAGACTTGGGCTTGCTGCTGCACCCTGGTGCCTACTTCCGGGACCTGTGGAACATTCTGGACTTCATTG
  TTGTCAGTGGAGCCCTGGTGGCATTTGCGTTCTCAGGATCCAAAGGGAAAGACATCAATACCATCAAGTC
  TCTGAGAGTCCTGCGTGTCCTGAGGCCCCTCAAGACCATCAAGCGGCTGCCTAAGCTCAAGGCTGTCTTT
  GACTGTGTGGTGAACTCCCTGAAGAACGTCTTGAACATCCTGATTGTCTACATGCTCTTCATGTTCATAT
  TTGCCGTCATTGCCGTCCAGCTCTTCAAAGGGAAGTTCTTTTACTGTACTGATGAATCCAAGGAGCTGGA
  GAGGGACTGCCGGGGTCAGTATTTGGATTATGAGAAGGAAGAAGTAGAAGCCCAGCCAAGGCAGTGGAAG
  AAATATGACTTCCACTATGACAATGTTCTCTGGGCCTTGTTGACGCTGTTCACAGTGTCCACGGGAGAGG
  GGTGGCCCATGGTGCTGAAACACTCTGTGGATGCCACCTATGAGGAACAGGGGCCCAGTCCCGGGTTCCG
  GATGGAGCTCTCCATCTTCTACGTGGTCTACTTTGTGGTCTTCCCTTTTTTCTTTGTCAACATCTTTGTG
  GCCTTGATCATTATCACCTTCCAGGAACAGGGAGATAAGGTGATGTCTGAATGCAGCTTAGAAAAGAATG
  AGAGGGCTTGCATTGATTTTGCCATCAGTGCCAAGCCCCTGACACGGTACATGCCTCAAAACAAACAGTC
  GTTCCAGTATAAGACATGGACATTCGTGGTCTCTCCACCCTTTGAGTACTTCATCATGGCTATGATAGCC
  CTCAACACAGTGGTGCTGATGATGAAGTTCTATGATGCACCTTATGAGTACGAGCTGATGCTGAAATGCC
  TGAACATTGTCTTCACATCCATGTTCTCGATGGAGTGCATACTGAAGATCATCGCCTTTGGGGTATTGAA
  CTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACGGTTTTGGGAAGTATTACTGATATTTTAGTA
  ACAGAGATAGCGGAAACGAACAACTTCATCAACCTAAGCTTCCTTCGCCTCTTCCGGGCGGCACGGCTGA
  TCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCCTATTGTGGACCTTCGTCCAGTCCTTTAAGGCGCT
  GCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTACGCCATCATCGGCATGCAGGTTTTTGGA
  AACATTGCCCTTGATGATGACACCAGTATCAACCGACACAACAACTTCCGGACATTTCTGCAAGCCTTAA
  TGCTATTGTTCAGGAGTGCCACTGGGGAGGCCTGGCATGAGATCATGCTGTCTTGTCTGGGCAACCGGGC
  CTGTGACCCACATGCCAACGCCAGTGAGTGCGGGAGCGACTTTGCCTATTTTTATTTTGTCTCCTTCATC
  TTCCTCTGTTCCTTTCTGATGTTGAACCTCTTTGTTGCTGTAATCATGGACAATTTTGAGTACCTCACTC
  GGGACTCTTCCATCCTAGGGCCTCACCACTTAGACGAATTCATTCGAGTCTGGGCTGAATACGACCCAGC
  TGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTGAAACACATGTCCCCACCTCTGGGGTTG
  GGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTCGCATGAACATGCCCATATCCAATGAGG
  ACATGACGGTGCACTTTACGTCCACACTGATGGCCCTCATCCGGACAGCACTGGAGATCAAGCTTGCCCC
  AGCTGACGAGATGACAGTGGGGAAGGTCTATGCTGCTCTCATGATATTTGACTTCTACAAACAGAACAAA
  ACCACCAGAGATCAGACTCACCAAGCTCCCGGAGGCCTGTCCCAGATGGGTCCCGTTTCCCTGTTCCACC
  CTCTGAAGGCCACCCTGGAACAGACACAGCCCGCTGTGCTTCGAGGAGCTCGGGTTTTCCTTCGGCAAAA
  GAGTGCAACTTCCCTCAGCAATGGGGGTGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCGCTGTCC
  TGGGGCACGCAGAGGACCCAAGATGCACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAG
  AGATCCCTGTGGGGCAGTCAGGAACACTGGCTGTGGATGTCCAGATGCAGAACATGACACTGAGAGGACC
  AGATGGGGAGCCCCAGCCTGGGCTGGAAAGCCAAGGCAGAGCTGCCTCTATGCCACGCCTAGCGGCAGAA
  ACACAGCCGGCCCCTAATGCCAGCCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCACATGGGA
  CTCAGCTTTGCAGCACAGTTCTGGACCGGCCTCCTCCTAGCCAGGCATCACATCACCACCACCACCGCTG
  CCACCGGCGCAGAGACAAGAAGCAAAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACCCAGAAGGT
  GCACCAAGCACTGCTGCAGGACCTGGTCTGCCCCATGGAGAAGGATCCACCGCCTGCCGGCGGGACCGTA
  AACAGGAGCGAGGCCGGTCCCAGGAGCGGAGGCAGCCCTCATCTTCCTCTTCAGAGAAGCAGCGCTTCTA
  TTCCTGTGACCGCTTTGGGAGCCGGGAGCCCCCGCAACTGATGCCCTCACTCAGTAGCCACCCCACATCG
  CCAACAGCGGCGCTAGAGCCAGCACCCCACCCACAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGT
  CAACATCCGGTGCTAGCACCCCGGGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCTCTGACCCCACG
  CCCCAGCATCACCTACAAGACCGCCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGCGGCCTCCCA
  GCCTTCTCCCCTGGCCGTCTCAGCCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGA
  GCCAGCCTCTAGCTCCTGGCTCCCGAATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGC
  CTCCGCCCACACCCTGCCTGAGGATACACTCACCTTTGAAGAGGCAGTGGCCACCAACTCTGGCCGCTCC
  TCCAGGACTTCCTATGTGTCCTCCCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTATC
  ACTGCACTTTGGGACTCAGCACTGGCGTCCGGGCGCGGCACAGCTACCACCACCCCGATCAGGACCACTG
  GTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007579
• Insert Size: 6867 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007579.3, NP_031605.2
• RefSeq Size: 9655 bp
• RefSeq ORF: 6867 bp
• Locus ID: 12287
• Cytogenetics: 2 16.58 cM
• Gene Summary: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are specifically blocked by omega-conotoxin-GVIA (AC P01522) (By similarity). They are however insensitive to dihydropyridines (DHP). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.