Pole (NM_011132) Mouse Untagged Clone

CAT#: MC225257

Pole (untagged) - Mouse polymerase (DNA directed), epsilon (Pole), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pole
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225257 representing NM_011132
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTGAGGAACAGTGGACGGAGGCACCCCGAGCCGGGCGCGGATGGCGAAGGCAGCCGGGATGATG
  GTCCCTCTTCCTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACAGACAAGATGGACTTACG
  GTTTGGTTTCGAAAGGCTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTGATCAACATGCACCCTACTGAG
  ATCTTAGATGAAGACAAACGCTTAGTCAGCGCGGTGGATTACTACTTCATTCAAGATGATGGAAGCAGAT
  TTAAGGTGGCCTTGCCCTATATGCCGTATTTCTACATTGCAGCGAGAAAGGGTTGTGATCGAGAAGTTTC
  ATCTTTTCTATCCAAGAAGTTTCAGGGAAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGATCTGGAC
  TTGCCAAATCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGACCTTG
  TCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGATGAGTA
  TACAACAATGCTCTCCAGTATTCTGCAAGGTGGCAGTGTAATTACTGATGAGGACGAAACCTCTAAGAAG
  ATAGCTGACCAATTGGACAACATAGTGGACATGCGAGAGTATGATGTTCCCTACCACATTCGCCTCTCCA
  TTGACCTCAGAATCCATGTGGCCCACTGGTACAATGTTAGATTTCGAGGAAATGCTTTTCCTGTGGAAAT
  CACCCGACGAGATGATCTTGTTGAACGACCTGACCCTGTGGTTTTGGCATTTGACATCGAGACGACCAAA
  CTGCCTCTCAAATTCCCTGATGCTGAGACCGATCAGATCATGATGATCTCCTATATGATTGATGGCCAGG
  GCTACCTCATCACTAACAGGGAGATTGTTTCAGAAGATATTGAAGATTTTGAGTTCACCCCTAAGCCAGA
  ATATGAAGGGCCCTTTTGTGTCTTCAATGAACCCGACGAGGTCCATCTGATCCAGAGATGGTTTGAGCAT
  ATCCAGGAAACCAAACCTACCATTATGGTCACCTACAATGGGGATTTTTTTGACTGGCCATTTGTGGAGG
  CTAGGGCAGCAATTCATGGCCTCAGCATGTACCAGGAGATAGGCTTCCAGAAGGATAGCCAGGGGGAATA
  TAAGGCACCACAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGTGGGC
  AGTCATAATCTCAAGGCAGCTGCCAAGGCCAAACTTGGCTATGACCCTGTAGAGCTGGACCCTGAGGACA
  TGTGTCGTATGGCCACTGAACAGCCCCAGACTCTGGCCACTTACTCAGTGTCAGATGCTGTGGCTACTTA
  CTACCTGTACATGAAATACGTCCACCCCTTCATATTCGCCCTGTGCACCATTATTCCCATGGAACCTGAT
  GAGGTGCTGCGGAAGGGCTCCGGGACACTGTGTGAAGCCTTGCTGATGGTGCAAGCTTTCCATGCCAACA
  TTATCTTCCCCAATAAGCAAGAGCAGGAGTTCAACAAGCTGACAGATGATGGCCACATGCTAGATGCTGA
  GACCTACGTCGGGGGCCACGTGGAGGCACTAGAGTCTGGTGTCTTCAGAAGTGATATCCCCTGCCGGTTT
  AGGATGAATCCTGCAGCCTTTGATTTCCTGCTGCAACGAGTCGAGAAGACTATGCGCCACGCCATTGAAG
  AAGAAGAGAAGGTGCCTGTGGAACAAGCCACCAACTTTCAAGAGGTGTGTGAGCAGATTAAGACCAAGCT
  CACCTCCCTAAAAGATGTTCCTAACAGAATTGAATGTCCTCTAATCTACCATCTAGATGTGGGGGCCATG
  TATCCTAACATAATTCTTACCAACCGCCTACAGCCTTCTGCCATAGTGGATGAGGCCACCTGTGCTGCCT
  GTGACTTCAATAAGCCTGGAGCAAGTTGTCAGAGGAAGATGGCCTGGCAGTGGAGGGGAGAATTCATGCC
  AGCCAGTCGCAGTGAATACCATCGGATTCAGCATCAGCTGGAGTCGGAGAAGTTTCCCCCTTTGTTTCCA
  GAGGGGCCAGCACGGGCCTTTCACGAGCTGTCCCGTGAAGAACAGGCTAAATACGAGAAGAGGAGGCTGG
  CAGATTATTGCCGGAAAGCCTATAAGAAGATCCATGTGACCAAGGTAGAAGAACGTCTAACTACCATCTG
  CCAGCGGGAAAACTCATTTTATGTGGACACAGTGCGGGCCTTCAGAGACAGGCGCTATGAGTTCAAAGGA
  CTGCACAAGGTGTGGAAGAAGAAGCTCTCGGCAGCTGTAGAGGTGGGCGATGCATCAGAGGTGAAGCGCT
  GCAAGAACATGGAGATCCTTTACGATTCACTGCAGCTGGCTCACAAGTGCATCCTGAACTCCTTCTACGG
  CTATGTCATGCGCAAAGGAGCTCGCTGGTATTCCATGGAGATGGCTGGTATCGTCTGCTTTACAGGAGCC
  AACATCATCACCCAAGCAAGAGAACTGATTGAGCAGATCGGGAGGCCTTTAGAATTGGACACGGACGGAA
  TATGGTGCGTCCTACCCAATAGCTTTCCTGAAAATTTTGTCATCAAGACAACCAATGCGAAGAAACCCAA
  ACTGACCATCTCCTATCCTGGTGCCATGTTGAACATCATGGTCAAGGAAGGCTTTACCAACCACCAGTAC
  CAGGAACTAACAGAGCCTTCGTCTCTCACCTATGTCACCCACTCTGAGAATAGTATCTTTTTTGAAGTCG
  ATGGACCATACCTTGCTATGATCCTTCCAGCCTCCAAGGAAGAAGGCAAGAAGCTGAAGAAAAGATATGC
  TGTGTTCAATGAAGATGGTTCCTTGGCTGAACTGAAAGGTTTTGAGGTGAAACGCCGAGGGGAGTTGCAG
  CTGATTAAAATATTCCAGTCCTCAGTTTTTGAGGCCTTCCTCAAGGGCAGCACACTGGAGGAAGTGTATG
  GCTCGGTGGCCAAAGTGGCTGACTACTGGCTAGATGTGCTCTATAGCAAGGCTGCTAATATGCCCGATTC
  TGAATTGTTTGAGCTGATTTCTGAGAACCGCTCCATGTCTCGGAAGCTGGAAGATTACGGGGAGCAGAAG
  TCTACATCCATCAGCACAGCAAAGCGCCTGGCTGAGTTCCTGGGAGACCAGATGGTCAAAGATGCTGGAC
  TGAGCTGCCGCTATATCATCTCCCGAAAGCCAGAGGGGTCTCCTGTCACTGAGAGGGCCATTCCACTTGC
  CATTTTCCAAGCAGAGCCTACAGTGAGGAAACATTTTCTCCGGAAATGGCTAAAGAGTTCATCACTTCAA
  GACTTTGATATTCGGACAATTCTGGACTGGGACTACTACATAGAGAGGCTGGGGAGTGCCATCCAGAAAA
  TCATCACCATCCCCGCAGCTCTGCAGCAGGTGAAGAACCCAGTTCCACGTGTCAAACATCCAGACTGGCT
  ACACAAAAAACTACTAGAGAAGAATGATATCTACAAACAGAAGAAGATCAGTGAGCTCTTTGTGCTTGAA
  GGAAAGAGACAGATTGTGATGGCCCAGGCTTCAGAAAACAGTCTGAGTCTCTGCACTCCAGACATGGAGG
  ACATTGGACTCACAAAGCCACACCACTCTACAGTCCCAGTTGCTACTAAGAGGAAGCGAGTCTGGGAGAC
  CCAAAAGGAGTCTCAGGATATTGCACTAACTGTGCCCTGGCAAGAGGTCTTAGGGCAGCCTCCCTCCCTT
  GGAACCACACAGGAAGAGTGGTTGGTCTGGCTCCAGTTCCACAAGAAAAAGTGGCAGCTGCAGGCCCAAC
  AGCGCCTAGCCCTCAGGAAGAAGCAACGCTTAGAGTCAGCAGAAGATATGCCAAGGCTTGGGCCTATCCG
  AGAGGGGCCTTCCACAGGACTGGGGAGCTTTTTGCGAAGGACTGCCCGCAGCATCATGGACCTTCCATGG
  CAGATAATACAGATCAGTGAGACCAGACAGGCTGGTCTGTTCCGGCTGTGGGCTATCATTGGCAATGACT
  TGCACTGCATCAAGCTGAGTATCCCTCGAGTATTCTATGTAAACCAGCGGGTTGCCAAAGCAGAGGATGG
  ACCTGCATATCGGAAGGTGAATCGGGCTCTTCCTCGTTCCAACATTGTCTACAATCTCTATGAGTATTCA
  GTACCAGAGGACATGTACCAAGAACACATCAACGAGATCAACACTGAGTTGTCAGTACCAGACATTGAGG
  GCGTGTATGAGACACAGGTCCCATTGTTATTCCGGGCCCTCGTGCAGCTGGGCTGTGTGTGTGTGGTCAA
  CAAGCAGCTGACAAGGCACCTTTCGGGCTGGGAAGCTGAAACTTTTGCCCTCGAGCACCTTGAAATGCGT
  TCTCTGGCCCAGTTCAGCTACTTGGAACCAGGGAGTATCCGCCATATCTACCTGTACCATCACACTCAGG
  GCCACAAGGCACTCTTTGGGGTCTTTATCCCCTCACAGCGAAGAGCATCTGTGTTTGTGTTGGATACTGT
  ACGAAGCAACCAAATGCCAGGGCTCAGTGCCCTGTACTCATCAGAACACAGCCTGCTGCTGGACAAGGTG
  GACCCCAAGCTCCTGCCTCCCCCAAAACACACCTTTGAAGTTCGTGCTGAAACCAACCTGAAGACTATCT
  GCAGAGCCATCCAGCGCTTCCTGCTTGCCTACAAGGAAGAGCGCCGAGGGCCCACACTCATCGCTGTCCA
  GTCTAGCTGGGAGCTGTGTAGGCTGACCAGTGAGATTCCAGTCTTAGAAGAGTTCCCACTAGTGCCTATC
  CGAGTGGCTGACAAGATCAGCTATGCAGTCCTGGACTGGCAGCGCCATGGAGCTCGCCGAATGATCCGGC
  ACTACCTCAATTTAGACTTGTGCCTGTCGCAGGCCTTTGAGATGAGCAGGTACTTCCACATCCCTGTTGG
  AAACCTGCCGGAAGACATCTCCACCTTTGGCTCAGACCTCTTTTTTGCACGCCACCTCCAGCACCATAAC
  CACCTGCTTTGGCTATCCCCTACCTCTCGGCCTGACCTGGGTGGGAAGGAAGCTGATGACAACCGCCTTG
  TCATGGAGTTTGATGACCGAGCCACTGTGGAGATCAATAGTTCTGGCTGTTACTCTACTGTGTGCGTGGA
  ACTGGACATTCAAAATCTGGCAGTCAACACCATCCTCCAGTCCCATCATGTCAATGACATGGAGGGGGCT
  GGCAGCATGGGCATCAGCTTCGATGTGATCCAGCAGGCCTCCCTAGAGGACATGGTAACAGGCAATCAAG
  CTGCCAGTGCCCTGGCCAACTACGATGAGACAGCCCTCTGCTCTAGTACCTTCAGGATCCTGAAGAGCAT
  GGTGGTTGGCTGGGTAAAGGAAATCACACAGTACCACAACATCTATGCTGACAACCAGGTAATGCACTTC
  TACCGCTGGCTCCAGTCACCGTGCTCTCTGCTCCACGACCCAGCCCTTCACCGGACGCTGCACAACATGA
  TGAAGAAGCTCTTCCTGCAGCTCATTGCTGAGTTCAAGCGCCTGGGGTCATCAGTCGTCTATGCCAACTT
  CAATCGCATCATTCTCTGTACAAAGAAGCGCCGAATAGAGGATGCCCTTGCCTATGTGGAATATATTACC
  AACAGCATCCATTCTAAAGAGATCTTCCATTCCCTGACCATCTCTTTCTCTCGATGCTGGGAATTCCTTC
  TCTGGATGGATCCATCCAACTATGGTGGAATCAAAGGAAAAGTTCCATCTAGTATTCACTGTGGACAGGT
  AAAAGAGCAAGACTCCCAGGCAAGAGAGGAAACTGATGAAGAGGAGGAGGACAAGGAAAAGGACGAGGAG
  GAAGAGGGCATGGGAGAGTCCGAGGTTGAGGACTTACTGGAGAACAACTGGAACATTCTACAGTTCTTGC
  CCCAGGCAGCCTCTTGCCAGAGCTACTTCCTCATGATTGTTTCAGCATACATCGTAGCTGTGTACCAAAG
  CATGAAGGAGGAGTTGAGACACAGTGCCCCGGGCAGTACCCCTGTGAAGAGGAAGGGGGCCAGCCAGTTC
  TCCCAGGAGTCTGAAGGGGCAACTGGATCTCTTCCTGGAATGATCACTTTCTCTCAAGATTATGTGGCAA
  ATGAGCTCACTCAGAGCTTCTTCACCATTACTCAGAAAATTCAGAAGAAAGTCACAGGTTCTCGGAACAC
  CACTGAGCCCTCAGAGATGTTCCCCGTCCTCCCTGGTTCACACTTGCTGCTCAATAATCCTGCTCTGGAG
  TTCATCAAATATGTGTGCAAGGTACTATCTCTGGATACAAACATCACAAATCAGGTGAATAAGCTGAACA
  GAGACCTTCTTCGCCTGGTAGACGTTGGTGAATTCTCTGAGGAGGCCCAGTTCAGAGACCCCTGCCACTC
  CTACGTGCTCCCTGAGGTAATCTGCCACAGCTGTAATTTCTGCCGAGACCTGGACCTGTGCAAAGATTCC
  TCTTTCTCTCAGGATGGAGCCATCCTGCCTCAGTGGCTCTGCTCCAATTGTCAAGCCCCCTATGACTCCT
  CTGCCATTGAGTCAGCCTTGGTGGAAGCCCTGCAGAGGAAACTGATGGCCTTCACACTTCAGGACCTGGT
  ATGCCTCAAGTGCCGTGGTATGAAAGAGACCCATATGCCTGTGTACTGCAGCTGCGCAGGGGACTTTACT
  CTCACCATCCGCACTGAGGTCTTCATGGAACAGATTAGAATCTTCCAGAACATTGCCAAGTACTACAGCA
  TGTCATATCTCCAGGAGACCATAGAATGGCTGTTACAGACAAGCCCTGTATCAAACTGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011132
• Insert Size: 6852 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011132.2, NP_035262.2
• RefSeq Size: 7167 bp
• RefSeq ORF: 6852 bp
• Locus ID: 18973
• UniProt ID: Q9WVF7
• Cytogenetics: 5 53.45 cM
• Gene Summary: Participates in DNA repair and in chromosomal DNA replication.[UniProtKB/Swiss-Prot Function]