Ascc3 (NM_198007) Mouse Untagged Clone

CAT#: MC225222

Ascc3 (untagged) - Mouse activating signal cointegrator 1 complex subunit 3 (Ascc3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ascc3
• Synonyms: ASC1p200; B630009I04Rik; BC023451; D430001L07Rik; D630041L21; Helic1; RNAH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225222 representing NM_198007
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTACCTCGGCTCACAGGAGCCTTGCGCTCCTTTTCAAATGTCACCAAGCAAGATAATTACAATG
  AAGAAGTAGCTGACTTGAAGTTGAAGCGATCTAAACTTCATGAACAAGTTTTAGATTTTGGCCTTACATG
  GAAGAAGATAGTGAAATTTTTGAATGAAAAGCTAGAGAAGAATAAAATGCAAAATATAAATGAAGACTTA
  AAAGACATACTACAAGCTGCAAAACAAATCGTTGGAACGGATAATGGGAGAGAAGCAATTGAGAGTGGGG
  CTGCATTTCTCTTTATGACATTTCACATGACGGACTCTGTTGGTTACATGGAGACAAAGGCTATCAGACA
  GACGTTTGGTCCCTTTCCATCGTCATCTGCTACTTCAGCTTGTAATGCCACTAATCGAATTATTTCTCAT
  TTTAGTCAAGATGATCTCACTGCTTTTGTCCAGATGGCAGAAAACCCATGTAACGATAGAGTGGTTTTTG
  GCAAAAACTTAGCATTTTCTTTTGACATGTATGATCTGGACCACTTTGATGAATTACCAATAAATGGTGA
  ATCCCAGAAAACTATAAGCTTAGACTACAAGAAGTTCCTGAATGAACAGTTCCAGGAACCCTACACTCCT
  GAGCTCAAGCCTGTGGAGAAAACAAATGGCTCCCTATTGTGGTGTGAAGTTGAGAAGTACTTAAACGCAA
  CGTTAAAGGAAATGACTGAAGCAGCAAGAGTTGAAGATCTTTGCTGTACTCTATATGACATGCTTGCTTC
  TGCTAAAAGCGGCGATGAACTTCAGGACGAGCTGTTTGAATTGCTGGGACCTGAAGGACTGGATCTGATT
  GAGAAGCTGCTCCAGAACAGAATTACAATTGTAGATCGGTTCCTTAATTCCTCGAGCGATCATAAGTTTC
  AAGTTCTCCAAGATAGTTGTAAAAAGATTTTAGGAGAGAATTCAAAGCCTAATTATGGATGTCAAGTCAC
  TATTCAATCTGAACAAGAAAAACAGTTAATGAAACAATATCGACGTGAAGAAAAAAGAATTGCCAGGCGA
  GAAAAAAAGGCTGGAGAAGATGGGGAAGTTTCTGGAGAAGGAGTTTTGCCCTTTGATCCTAAGGAACTCC
  GGATACAGAGAGAACATGCACTTCTGAATGCTAGAAATGCTCCAATTTTAGGAAGACAGAGAGACGTGGA
  GTTTGAAAAAATCCGTTATCCTCATGTTTATGATTCCCAGGCTCAGGCCCGAGAAACATCAGCATTCATA
  GCTGGAGCCAAGATGATTTTACCAGAAGGAATCCAGAGAGAGAATACCAAGCTGTATGAGGAAGTCAGGA
  TCCCCTATGGTGAACCAATGCCAGTTGGGTTTGAGGAAAAACCAGTTTATATCAAAGATTTAGATGAGGT
  TGGACAGTTGGCTTTTAAAGGAATGAAGAGACTCAATAGAATCCAGTCTATAGTATTTGAAACAGCCTAC
  AACACCAACGAAAACATGCTGATCTGTGCCCCTACGGGAGCTGGGAAGACCAATATTGCAATGCTTACTA
  TCTTACATGAAATCCGCCAACACTTTCATCAAGGTGTTATTAAAAAGAATGAGTTTAAGATTGTGTATGT
  CGCTCCAATGAAAGCCTTGGCAGCTGAGATGACAAATTACTTCAGCAAACGTCTAGAGCCACTGGGCATT
  GTTGTGAAAGAATTGACTGGAGATATGCAGTTGTCAAAAAGTGAAATTTTACGAACTCAGATGCTGGTGA
  CCACACCAGAAAAATGGGATGTAGTAACAAGAAAGAGTGTTGGGGATGTTGCTCTTTCCCAAATCGTAAA
  ACTCCTGATTCTTGACGAGGTGCACTTGCTGCATGAAGACAGAGGCCCAGTGCTAGAAAGCATAGTCGCC
  AGGACCTTACGGCAGGTTGAATCCACACAGAGTATGATAAGAATTCTTGGATTGTCTGCAACCTTACCCA
  ACTACCTCGATGTTGCTACATTTTTGCATGTTAACCCCTACATTGGACTTTTCTACTTTGATGGCCGTTT
  TCGACCTGTACCTCTTGGACAGACATTTTTAGGGATTAAAAGTACAAACAAGATGCAGCAGTTAAATAAC
  ATGGATGAAGTGTGCTACGAAAGTGTTCTGAAACAAGTAAAGGCTGGACACCAGGTGATGGTATTTGTAC
  ATGCTCGCAATGCCACTGTAAGGACCGCTATGTCTCTAATAGAGAGAGCAAAAAATAGTGGCCAAATTTC
  TTGCTTTCTACCCACCGAAGGGCCAGAGTACGGACATGCATTAAAACAGGTACAAAAGTCAAGAAATAAG
  CAAGTGCGAGAATTATTCTCCGATGGCTTTAGCATTCATCATGCCGGGATGCTTCGGCAAGACAGAAATT
  TGGTTGAAAATTTGTTTTCAAATGGGCATATCAAAGTCCTAGTCTGTACAGCCACATTAGCCTGGGGTGT
  CAATCTTCCTGCACATGCCGTGGTTATTAAGGGAACACAAATATACGCTGCCAAAAGAGGCTCCTTTGTT
  GACCTTGGAATTTTAGATGTCATGCAGATATTTGGCCGAGCTGGACGACCGCAATTTGACAAATTTGGGG
  AAGGAATCATTATAACAACCCACGATAAACTCAGCCATTACCTCAGTTTGCTCACTCAACAAAACCCAAT
  TGAGAGTCAGTTTCTGGAAAGTCTTGCAGATAACCTAAATGCAGAGATTGCGCTGGGGACAGTCACCAAC
  GTGGAAGAGGCAGTGAGGTGGATGAGTTACACTTACCTCTATGTGCGCATGCGAGCAAACCCGCTAGCAT
  ACGGCATCAGTCACAAAGCGTACCAGATTGACCCAACACTAAGGAAGCATCGAGAACAGCTCCTTATTGA
  AGTTGGACAAAAGTTGGATAAAGCAAAGATGATTCGTTTTGAAGAACGAACTGGCTATTTTTCTTCTACT
  GATTTGGGTAGAACTGCCAGCCACTTCTATATTAAATACAACACCATTGAGACATTTAATGAACTCTTTG
  ATGCACACAAAACAGAGGGTGATATCTTTGCTATTGTTTCCAAAGCTGAAGAATTTGATCAAATTAAGGT
  CAGAGAAGAAGAAATAGAAGAATTAGATGCCTTGTTAAACAATTTCTGTGAACTCTCAGCTCCAGGAGGT
  GTGGAAAATAGTTATGGGAAAATAAATATCCTACTTCAAACCTACATCAGCCGAGGAGAGATGGACAGTT
  TCTCCCTCATATCAGATTCTGCATATGTCGCCCAGAATGCAGCTAGAATTGTCCGTGCTCTCTTTGAAAT
  TGCACTGAGGAAACGTTGGCCTACCATGACCTACAGGCTTTTGAATCTTAGTAAAGTCATTGACAAGAGA
  CTCTGGGGTTGGGCTAGCCCTTTGAGACAATTTTCTGTGTTACCACCACACATTCTAACAAGATTAGAAG
  AAAAAAACCTTACTGTGGATAAACTCAAAGACATGAGAAAAGATGAAATAGGTCACATTTTGCATCATGT
  GAATATTGGACTGAAGGTCAAACAGTGTGTTCATCAAATTCCTTCTGTTACAATGGAAGCTTCCATTCAG
  CCCATCACACGGACTGTCCTCCGAGTATCACTGAACATCCACCCTGATTTCTCTTGGAATGACCAGGTTC
  ATGGAACAGTGGGGGAACCATGGTGGATTTGGGTAGAAGATCCTACAAATGACCATATTTATCACTCGGA
  GTACTTTCTAGCTCTTAAAAAACAGGTTATTAATAAGGAAGCTCAACTTCTGGTATTTACAATTCCTATT
  TTTGAGCCTTTGCCCTCTCAGTACTACATTCGAGCAGTGTCTGATCGATGGCTAGGAGCTGAAGCTGTTT
  GCATTATCAACTTTCAACATCTGATTCTACCAGAGAGACATCCTCCCCATACAGAATTATTGGACCTTCA
  ACCTTTACCAATTACAGCTTTGGGGTGCAAAGCATATGAAGCTCTATATAACTTCAGCCACTTTAACCCT
  GTACAGACGCAGATATTCCACACACTGTATCACACAGACTGTAATGTTCTGCTTGGCGCCCCCACCGGAT
  CAGGAAAGACAGTTGCAGCTGAACTAGCCATTTTCAGAGTTTTCAACAAATATCCTACTTCAAAGGCAGT
  ATATATTGCACCCCTCAAAGCTCTAGTACGTGAAAGAATGGATGATTGGAAAATTAGGATAGAAGAAAAA
  CTTGGTAAAAAAGTTATTGAACTAACTGGGGATGTGACTCCAGACATGAAATCCATTGCTAAGGCTGACC
  TTATTGTCACCACACCAGAAAAGTGGGATGGAGTTAGTCGAAGCTGGCAGAATAGAAGCTATGTTCAGCA
  GGTCAACATTCTCATCATAGATGAGATCCATCTGCTAGGGGAGGAGAGAGGCCCAGTTTTAGAAGTCATT
  GTATCTCGAACAAACTTTATCTCATCACACACAGAAAAACCAGTTAGAATAGTTGGACTGTCCACTGCAT
  TAGCTAATGCTAGAGATCTTGCTGACTGGCTCAACATTAAGCAGATGGGTTTGTTTAACTTCCGACCATC
  AGTTCGCCCAGTTCCACTGGAAGTTCACATTCAGGGTTTCCCAGGACAACATTACTGCCCACGCATGGCT
  AGTATGAACAAGCCTGCATTTCAGGCAATTAGAAGTCACTCTCCAGCCAAGCCTGTTTTGATATTTGTCT
  CATCAAGACGTCAAACACGACTTACAGCCTTAGAGTTGATAGCATTTCTGGCCACTGAAGAAGACCCAAA
  GCAGTGGTTGAATATGGATGAGCAAGAGATGGACAACATCATTGGAACAGTACGAGATTCCAACCTCAAG
  CTGACTCTAGCTTTTGGAATAGGAATGCATCATGCTGGCCTGCATGAAAGAGACCGAAAAACTGTAGAAG
  AGCTGTTTGTGAACTGTAAAGTTCAGGTTCTCATTGCTACAAGCACATTAGCATGGGGTGTAAACTTTCC
  AGCACACTTAGTAATTATTAAGGGAACTGAATATTATGATGGAAAGACAAGACGTTATGTGGATTTTCCC
  ATCACAGATGTGCTGCAGATGATGGGACGAGCTGGGAGACCTCAGTTTGATGACCAGGGCAAAGCTGTAA
  TTCTAGTTCATGATATAAAAAAAGATTTTTATAAAAAGTTTCTTTATGAACCTTTCCCAGTAGAATCAAG
  CTTATTAGGAGTGTTGTCTGACCACTTAAATGCAGAGATTGCTGGAGGTACAATAACATCAAAGCAAGAT
  GCAATGGATTATATCACCTGGACTTACTTTTTCCGGCGTCTTATCATGAATCCCAGCTACTACAGCTTGG
  GTGACGTCAGCCAGGACTCCATAAACAAGTTCCTGTCACATCTGATTGGGCAGTCTCTGGTTGAATTGGA
  GCTTTCCCACTGCATTGAAGTTGGAGAGGATAATCGCACCATTGAACCACTGACATGTGGTCGAATTGCC
  TCCTATTACTATTTGAAGCACAAAACTGTCAAAATGTTCAAGGACCGTTTGAAACCTGAGTGCAGCACTG
  AGGAACTGCTGTCAATTCTGAGTGATGCAGAAGAATACACAGACTTACCAGTAAGACACAATGAGGACCA
  TACGAATAATGAATTGGCCAAATGTCTTCCCATTGAATTAAATCCTCATTCATTTGACAGCCCTCACACC
  AAAGCCCATCTCTTGCTTCAAGCGCATCTCAGCCGAGCCATGCTGCCCTGCCCAGATTATGATACTGATA
  CCAAAACAGTCTTAGACCAAGCTCTCAGAGTATGCCAGGCAATGCTGGATGTGGCTGCAAGCCAGGGATG
  GCTAGTGACCGTCCTGAATATCACCCACCTGATTCAGATGGTGATCCAAGGCCGATGGCTCAAGGACTCT
  TCTCTTCTCACAATACCAAACATAGAGCAGCACCACCTTCACCTTTTCAGGAAATGGAAGCCACCTGTAA
  AGAGCTCACATGCTAAGTGCCGAACATCCATTGAGTGCCTTCCTGAGCTGATCCATGCCTGTGAAGGGAA
  GGACCATGTGTTCAGCTCCATGGTAGAGAAAGAGTTACAGCCTGCAAAAACAAAGCAGGCATGGAATTTC
  TTATCTCGCTTGCCAGTGATAAATGTTGGCATAAGTGTTAAAGGCTCATGGGATGACTTAGTTGAAGGAC
  ATAATGAACTCTCCATCTCAACTCTGACAGCAGACAAACGGGATGAAAACAAATGGATCAAGTTGCATGC
  TGATCAGGAGTATGTGCTTCAAGTCAGCCTACAGAGAGTCCATTTTGGGCTTCCAAAGGGAAAACATGAG
  AATCACGCAGTTACTCCTCGATTTCCAAAATTAAAAGATGAAGGCTGGTTTTTGATATTAGGAGAAGTGG
  ATAAGAGAGAGCTGATGGCTGTAAAACGAGTAGGATTTGTTCGAACACATCATGATGCTTCCATCTCGTT
  CTTTACTCCTGAAACACCTGGAAGATATATCTTTACCCTGTATCTCATGAGTGACTGTTACCTCGGCCTA
  GACCAGCAGTATGACATTTATCTCAATGTCATAAAAGCAAACATTTCTACAAAGGACTCTGATGTCTTCA
  CTGACTTGTCAGTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198007
• Insert Size: 6597 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198007.2, NP_932124.2
• RefSeq Size: 7477 bp
• RefSeq ORF: 6597 bp
• Locus ID: 77987
• UniProt ID: E9PZJ8
• Cytogenetics: 10 B3
• Gene Summary: 3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation.[UniProtKB/Swiss-Prot Function]