Med12 (NM_021521) Mouse Untagged Clone

CAT#: MC225218

Med12 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 12 homolog (yeast) (Med12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med12
• Synonyms: 230kDa; Mopa; OPA-1; Tnrc11; Trap230
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225218 representing NM_021521
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTTTCGGGATCTTGAGCTATGAACACCGACCCCTGAAGCGGCTGCGGCTGGGGCCTCCCGATG
  TGTACCCTCAAGATCCCAAACAGAAGGAGGATGAACTGACGGCTTTGAATGTAAAACAAGGTTTCAATAA
  CCAGCCTGCTGTCTCTGGGGACGAACATGGCAGTGCCAAGAACGTCAACTTCAATCCTGCCAAGATCAGT
  TCCAACTTCAGCAGCATCATCGCAGAGAAGTTAAGGTGTAATACTCTCTCTGACACTGGTCGCAGGAAGT
  CCCTAATGAACCAGAAGGACAACTTCTGGCTGGTGACTGCAAGATCTCAGAGTGCTATTAACACCTGGTT
  CACTGACCTGGCTGGCACCAAACCACTCACACACCTAGCCAAAAAGGTCCCCATTTTCAGTAAAAAGGAA
  GAAGTATTTGGGTATTTGGCCAAATACACGGTGCCTGTGATGCGGGCTGCCTGGCTCATTAAGATGACTT
  GTGCCTACTATGCAGCAATGTCTGAGACTAAGGTTAAGAAGAAAAATACTGCTGACCCCTTCACTGAATG
  GACTCAAATCATCACAAAGTACTTGTGGGAGCAGCTACAGAAGATGGCTGAGTACTATCGGCCAGGGCCT
  GCAGGAAGTGGAGGCTGTGGTTCTACTATAGGGCCTTTGCCCCATGATGTAGAGATGGCCATCAGGCAGT
  GGGATTACAATGAGAAGCTCGCCTTGTTCATGTTTCAGGATGGAATGCTGGACAGACATGAGTTCCTGAC
  TTGGGTGCTTGAGTGTTTTGAGAAAATACGCCCTGGAGAAGATGAATTGCTTAAATTGCTGCTTCCCCTA
  CTGCTGCGATACTCAGGGGAGTTTGTTCAGTCTGCCTATCTGTCCCGCCGCCTTGCCTACTTCTGTACCC
  GGAGATTGGCTCTCCAGCTGGATGGTGTGAGCAGCCACTCATCTCATGTCATAGCTGCTCAGTCAACAAG
  TTCTCTGCCCACTACCCCAGCACCTCAGCCCCCAACTAGCAGTACACCCTCGACTCCCTTTAGTGACCTG
  CTTATGTGCCCTCAGCATCGTCCCCTGGTTTTTGGCCTCAGCTGTATCCTTCAGACCATCCTTCTGTGTT
  GCCCTAGTGCCCTAGTTTGGCACTACTCATTGACTGATAGCCGAATTAAGACCGGCTCACCACTTGACCA
  CCTGCCTATTGCTCCTTCCAACTTGCCCATGCCAGAGGGTAACAGTGCCTTTACTCAGCAGGTCCGTGCA
  AAATTACGAGAGATCGAACAACAGATCAAGGAGCGTGGACAAGCAGTTGAGGTTCGCTGGTCTTTTGATA
  AGTGCCAGGAAGCTACTGCAGGTTTCACCATTGGACGGGTGCTCCATACTTTAGAAGTGCTGGATAGCCA
  TAGTTTCGAGCGCTCTGACTTTAGTAACTCTCTTGACTCCCTTTGTAATCGAATCTTTGGATTGGGGCCT
  AGTAAGGATGGTCATGAGATTTCCTCTGATGATGATGCTGTGGTATCATTATTGTGTGAATGGGCTGTAA
  GCTGCAAACGCTCAGGTCGGCATCGTGCTATGGTAGTAGCCAAACTCCTAGAGAAGAGACAAGCAGAAAT
  TGAGGCTGAGCGCTGCGGAGAATCTGAAGCAGCTGATGAGAAGGGCTCCGTCGCCTCTGGTTCCCTTTCC
  GCTCCTAGTGCACCCATTTTCCAGGATGTCCTCCTGCAGTTTCTGGATACACAGGCTCCCATGCTGACTG
  ATCCCCGAAGTGAAAGTGAGCGAGTAGAATTCTTTAACTTGGTACTGCTCTTCTGTGAACTGATCCGACA
  TGATGTCTTCTCCCATAATATGTACACCTGCACTCTCATCTCTCGGGGGGATCTTGCATTTGGAGCCCCT
  GGTCCTCGGCCTCCCTCTCCCTTTGATGATCCTACAGATGATCCAGAGCGCAAGGAGGCTGAAGGCAGCA
  GCAGCAGCAAGCTAGAGGACCCAGGGCTCTCTGAATCTATGGACATCGACCCTAGTTCCAGTGTGCTTTT
  TGAGGACATGGAAAAGCCTGATTTCTCATTGTTCTCCCCTACTATGCCTTGTGAGGGGAAGGGAAGCCCA
  TCCCCTGAGAAACCAGATGTCGAAAAGGAAGTAAAACCCCCAGCCAAAGAGAAGATCGAGGGGACACTTG
  GGATTCTGTATGACCAGCCACGACATGTGCAGTATGCCACACACTTTCCAATCCCACAGGAGGAGTCATG
  CAGCCATGAGTGCAACCAGCGGTTGGTCGTACTGTTTGGGGTGGGGAAGCAGCGAGATGATGCCCGCCAT
  GCCATCAAGAAGATTACCAAGGATATCCTGAAGGTTCTGAATCGCAAGGGGACAGCAGAAACTGACCAGC
  TTGCTCCTATTGTGCCTCTGAATCCTGGAGACCTGACATTCTTAGGTGGGGAAGATGGGCAGAAGCGACG
  ACGCAACCGGCCCGAAGCCTTCCCCACTGCTGAAGATATTTTTGCTAAGTTCCAGCACCTTTCCCATTAT
  GACCAACACCAGGTCACGGCTCAGGTCTCCCGGAACGTTCTGGAGCAGATCACGAGCTTCGCCCTTGGCA
  TGTCGTACCACTTGCCTCTGGTGCAGCATGTGCAGTTCATCTTCGACCTCATGGAATATTCACTGAGCAT
  CAGTGGCCTCATCGACTTTGCCATCCAGTTACTGAATGAGCTGAGTGTGGTTGAGGCGGAGCTCCTTCTC
  AAATCGTCGGATCTGGTGGGCAGCTACACTACCAGCCTGTGCTTATGTATCGTGGCTGTCCTTCGACACT
  ATCATGCCTGCCTCATCCTCAATCAGGACCAGATGGCGCAAGTGTTTGAGGGGCTCTGTGGCGTGGTGAA
  ACATGGAATGAACCGTTCAGATGGCTCCTCTGCAGAGCGCTGTATCCTTGCTTATCTCTATGATCTGTAT
  ACCTCCTGTAGCCATTTAAAGAGCAAATTTGGGGAGCTCTTCAGTGACTTTTGCTCAAAAGTGAAGAACA
  CCATCTACTGTAACGTGGAGCCATCGGAATCCAATATGCGCTGGGCACCGGAGTTCATGATTGACACTCT
  GGAGAACCCTGCCGCTCACACTTTCACCTACACGGGGCTCGGCAAGAGTCTTAGTGAGAACCCTGCTAAC
  CGCTATAGCTTTGTGTGCAATGCTCTTATGCACGTCTGCGTGGGCCACCATGATCCTGATAGGGTAAATG
  ACATCGCCATCCTTTGTGCAGAGCTGACCGGCTATTGCAAGTCCTTGAGTGCTGAGTGGCTAGGAGTACT
  TAAGGCCTTGTGCTGCTCCTCGAACAATGGCACTTGTGGTTTCAATGATCTCCTGTGCAACGTAGACGTC
  AGTGATTTGTCTTTTCATGATTCCTTGGCTACTTTTGTTGCTATCCTCATCGCCCGTCAATGTTTGCTCC
  TAGAAGACCTGATTCGCTGTGCAGCCATCCCTTCACTCCTCAATGCTGCTTGTAGTGAGCAGGATTCTGA
  GCCAGGAGCCAGGCTTACTTGCCGAATCCTCCTCCACCTGTTCAAGACGCCACAACTCAATCCTTGCCAG
  TCTGATGGAAACAAACCTACTGTTGGAATCCGGTCCTCCTGTGACCGCCACCTGCTGGCTGCCTCCCAGA
  ACCGCATCGTGGATGGGGCTGTGTTTGCTGTTCTCAAGGCTGTGTTTGTACTCGGAGATGCGGAGCTAAA
  AGGTTCAGGCTTTACTGTGCCGGGAGGAACAGAAGAACTTCCAGAAGAGGAGGGAGGAGGTGGTAGTAGC
  GGTCGGAGACAGGGTGGCCGCAACATCTCTGTGGAGACAGCAAGTCTGGATGTCTATGCCAAGTACGTGC
  TGCGAAGCATCTGCCAACAGGAATGGGTAGGAGAACGTTGCCTTAAGTCACTGTGTGAGGATAGCAATGA
  TCTACAAGACCCAGTGTTGAGTAGTGCCCAGGCCCAGCGCCTCATGCAACTTATCTGCTACCCACATCGA
  CTGCTGGACAATGAGGATGGAGAAAACCCCCAGCGGCAGCGCATTAAACGTATTCTCAAGAATTTAGACC
  AATGGACCATGCGCCAGTCCTCTTTGGAGCTACAGTTGATGATCAAGCAGACCCCCAACACTGAGATGAA
  CTCTCTCTTGGAGAACATTGCCAAGGCCACAATCGAGGTTTTCCAACAGTCAGCAGAGACGGGGTCATCT
  TCTGGAAGTACAGCAAGCAACATGCCCAGCAGCAGCAAGACCAAACCTGTGCTCAGCTCTCTAGAGCGAT
  CTGGTGTATGGTTGGTGGCTCCTCTCATTGCCAAACTGCCCACTTCAGTCCAGGGCCATGTATTAAAAGC
  TGCTGGGGAGGAACTGGAAAAGGGTCAGCACCTGGGCTCTTCTTCCCGTAAAGAACGAGATCGACAGAAA
  CAGAAGAGCATGTCCCTGTTGAGCCAACAGCCTTTCTTATCACTGGTGCTAACATGTCTGAAAGGACAGG
  ATGAGCAGCGCGAGGGACTCCTGGCCTCCCTCCACAGCCAGGTGCACCAGATTGTGATTAATTGGCGAGA
  AAACCAGTACTTAGATGATTGCAAACCAAAGCAGCTAATGCATGAGGCACTCAAACTGCGGCTCAACCTG
  GTGGGGGGCATGTTTGACACTGTGCAGCGTAGTACCCAGCAGACTACAGAGTGGGCCCAGCTTCTCCTTG
  AGATCATCATCAGCGGCACTGTGGACATGCAGTCTAACAATGAGCTCTTCACTACTGTGTTGGACATGCT
  GAGCGTGCTTATCAACGGAACGTTGGCTGCAGACATGTCCAGTATCTCGCAAGGCAGCATGGAGGAAAAC
  AAGCGTGCATATATGAACCTGGTGAAGAAGCTTCAGAAGGACTTGGGGGAGCGCCAATCAGACAGTCTGG
  AGAAGGTTCACCAACTGTTGCCACTACCCAAGCAGAACCGAGATGTCATAACCTGTGAGCCACAGGGCTC
  CCTTATTGACACCAAGGGCAACAAGATTGCTGGCTTCGATTCCATCTTCAAGAAGGAGGGTCTACAAGTT
  TCTACCAAACAAAAGATCTCTCCCTGGGAGCTTTTTGAGGGCCTGAAGCCATCAACAGCACCACTGTCAT
  GGGCCTGGTTTGGCACAGTCCGAGTGGACCGCAGAGTGGCACGAGGGGAGGAGCAGCAGCGGCTGTTGCT
  CTATCATACCCACCTGAGGCCTCGACCCAGAGCCTATTACCTGGAACCACTACCTCTGCCCCCAGAAGAT
  GAGGAGCCACCAGCCCCTGCCCTACTAGAGCCTGAGAAAAAGGCTCCTGAGCCCCCCAAGACTGACAAAC
  CAGGGGCTGCTCCTCCGAGCACTGAGGAGCGCAAAAAGAAGTCTACCAAGGGCAAAAAACGCAGCCAGCC
  AGCCACCAAGAACGAGGACTATGGCATGGGGCCAGGTCGGAGTGGCCCCTATGGTGTGACAGTGCCTCCA
  GACCTTCTACACCATGCAAATCCTGGTTCTATATCCCACCTTAGCTACAGGCAAAGCTCCATGGGCCTGT
  ATACCCAAAACCAGCCACTACCTGCTGGTGGCCCTCGTGTGGATCCATACCGCCCCGTGCGATTACCAAT
  GCAAAAGCTGCCAACTCGACCAACTTATCCCGGTGTGCTGCCTACAACTATGTCTACTGTCATGGGCCTA
  GAACCCTCTTCTTATAAGACATCTGTATACCGGCAGCAGCAACCCACAGTGCCCCAGGGACAGCGCCTTC
  GCCAACAGCTCCAGGCAAAGATACAGAGTCAGGGGATGTTGGGACAGTCATCTGTCCATCAGATGACCCC
  TAGTTCTTCCTATGGTTTGCAGACTTCCCAGGGCTATACATCCTATGTTTCTCATGTGGGATTGCAGCAA
  CACACAGGCCCTGCAGGTACCATGGTGCCCCCCAGCTACTCCAGCCAGCCTTATCAGAGCACCCACCCTT
  CTACCAATCCTACTCTTGTAGATCCTACCCGCCACCTGCAACAGCGGCCCAGTGGCTATGTGCATCAGCA
  GGCCCCAACCTATGGGCATGGACTGACTTCCACTCAAAGGTTTTCACACCAGACACTGCAGCAGACACCC
  ATGATGGGTACCATGACTCCGTTGAGTGCCCAGGGTGTCCAGGCAGGCGTCCGTTCAACTTCCATCCTGC
  CTGAGCAGCAGCAACAACAACAGCAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACA
  ACAGCAGCAGCAGCAGCAGCAACAACAACAGCAGTACCATATCCGACAGCAACAGCAGCAGCAGCAGATG
  CTACGGCAACAGCAGCAACAACAGCAACAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACAACAGC
  AACAGCAGCAGCAGCAGCCACACCAGCAGCAGCAGCAGGCAGCTCCTCCCCAACCCCAGCCCCAGTCCCA
  GCCCCAGTTCCAGCGCCAGGGCCTGCAGCAGACCCAGCAGCAGCAACAGACAGCAGCTTTGGTCCGGCAA
  CTTCAACAACAGCTCTCTAATACCCAGCCACAGCCCAGCACCAACATATTTGGACGCTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021521
• Insert Size: 6573 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021521.2, NP_067496.2
• RefSeq Size: 8464 bp
• RefSeq ORF: 6573 bp
• Locus ID: 59024
• UniProt ID: A2AGH6
• Cytogenetics: X D
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]