Med12l (NM_177855) Mouse Untagged Clone

CAT#: MC225217

Med12l (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 12 homolog (yeast)-like (Med12l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med12l
• Synonyms: A130035F20; A630079M02; AU044581; TRALPUSH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225217 representing NM_177855
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCTTCGGGCTTCTCAGCTATGAGCAGAGACCGCTGAAGCGCCCGCGGCTCGGGCCGCCCGACG
  TCTACCCGCAGGACCCTAAGCAGAAGGAGGATGAACTTACAGCTGTGAATGTAAAGCAGGGATTCAACAA
  CCAGCCGGCCTTCACTGGAGACGAACATGGCTCAGCCAGAAACATTGTAATCAACCCATCCAAGATCGGT
  GCGTATTTCAGCAGCATATTGGCCGAGAAACTGAAGCTTAACACTTTCCAGGACACCGGAAAGAAGAAAC
  CCCAAGTTAATGCTAAAGATAATTACTGGCTGGTTACTGCCCGATCGCAGAGTGCAATTCATAGTTGGTT
  CTCTGACCTAGCAGGGAATAAACCACTTGCTATCTTGGCAAAAAAGGTCCCCATCCTCAGTAAAAAAGAG
  GACGTCTTTGCATATTTAGCTAAGTATTCTGTGCCAATGGTTCGAGCAACGTGGCTGATCAAGATGACTT
  GTGCCTATTACTCTGCTATTTCTGAAGCTAAAATTAAGAAACGCCAGGCTCCCGATCCCAATTTGGAGTG
  GACACAGATATCTACCAGATACCTCAGAGAGCAGTTGGTCAAAATCTCTGACTTTTACCACATGGCTTCC
  AGTACAGGTGATGGTCCTGTCCCTGTGCCACCAGAGGTTGAGCAAGCCATGAAGCAGTGGGAGTACAACG
  AGAAGCTGGCATTTCATATGTTCCAGGAAGGAATGTTGGAAAAGCACGAGTATTTGACTTGGATCCTGGA
  CGTCTTAGAGAAGATCAGACCAGTGGATGACAGCCTTCTGAAGCTACTTTTGCCCCTGATGTTGCAGTAT
  TCAGATGAGTTTGTTCAGTCAGCCTACCTGTCTCGTCGTCTCGCGTACTTTTGTGCCCGGCGTCTTTCCT
  TGCTACTGAGTGATAGCCCCAACCTCCTTGCTGCTCACTCGCCCCACATGATAATCGGAACAAACAACAC
  GAGTATTGGGACCCCCAGCCCTGGCACCCCTGGCCCTGGCATGAGCCCTGTGCAGCTGGCCTTCTCGGAT
  TTCCTTTCCTGTGCACAGCATGGCCCCCTGGTTTATGGACTTAGTTGTATGTTGCAGACTGTCACTCTGT
  GTTGCCCAAGTGCCTTGGTGTGGAATTATTCCACCAATGAAAATAAGATCTCGAATCCAGGCTCTCCCCT
  GGATCTGCTGCAGGTCGCCCCGTCCAGCCTCCCCATGCCGGGTGGGAACACGGCTTTCAATCAGCAGGTT
  CGGGCAAGGATTTATGAGGTAGAACAGCAGATAAAGCAGCGAGGCCGTGCAGTGGAAGTTCGGTGGTCTT
  TTGATAAGTGTCAGGAGTCGACGGCAGGGGTGACTATTAGTCGGGTCTTGCATACACTGGAAGTCTTAGA
  TCGTCATTGTTTCGACCGAACTGATTCCAGCAATTCAATGGAGACTCTTTATCATAAAATTTTCTGGGCA
  AACCAAAACAAAGATAACCAAGAGGTTGCCCCCAATGATGAAGCTGTGGTGACATTGTTATGTGAATGGG
  CTGTAAGCTGCAAAAGGTCTGGTAAGCACAGGGCCATGGCTGTGGCGAAACTCTTGGAGAAAAGGCAAGC
  GGAGATTGAGGCAGAGAGATGTGGGGAATCAGAAGTCCTAGATGAGAAAGAGTCAATTTCTTCAGCATCT
  CTTGCAGGATCTAGTTTGCCTGTTTTCCAGAATGTTTTGCTGAGGTTTTTAGATACACAGGCCCCAGCGC
  TGTCGGACCCCAACAGTGAATGCGAGAAGGTAGAGTTTGTGAACTTGGTGCTGCTCTTCTGCGAGTTTAT
  CCGCCACGATGTCTTCTCCCATGATGCATATATGTGCACCCTCATCTCTCGAGGAGACTTATCAGTTACT
  GCCTCCACGGGGCTGCGGTCGCCAGCAGGAGAGAATTCAGATGAACACTATTCCAAGGATCATGATATGA
  AGATGGAGGAGCACAGTGTCATGGCGCGCCTGGGTCTCGGCTCAGGAGCCACTCACATCTTTGATGATGT
  AGACAAGAGTGACTTTAAAAGTGACCCTGCTTCTGAGTTTCCAATTTTTTCTCCCATGCCTGGAGAATCC
  TGTGAGAACATCAACCCTTCCTTGAGCAGAAGACTGTCAGTCAATGGTGAGAAACTGCTAAAGAGAGAAA
  AACCAAGAGAATTAATTTTCCCATCAAATTACGACCTCCTCCGACACCTGCAGTATGCAACCCACTTTCC
  TATACCTCTGGATGAATCTTCAAGTCATGAATGTAACCAGCGCACAATCCTTCTCTATGGAGTCGGAAAA
  GAGCGTGATGAAGCCAGGCATCAGCTGAAGAAGATTACCAAAGATATCCTCAAAATCCTGAACAAGAAGG
  GCACCACAGAATCAGGGGTTGGGGACGAAGGACAGAAAGCCAGGAAGACCAAGCAGGAAGTTTTTCCAAC
  CCCGGAAAATGTGTTCACGAAACTCCAACTCCTCTCATACTTCGATCAGCATCAAGTAACATCTCAGATC
  TCTAACAATGTGCTGGAACAAATCACAAGCTTTGCGTCTGGAACATCCTATCACCTCCCGTTGGCTCACC
  ATATTCAGCTCATCTTTGATCTCATGGAGCCAGCGCTGAACATCAATGGGCTGATTGACTTTGCCATACA
  GCTGCTGAATGAACTGAGTGTGGTGGAAGCAGAGCTCCTCTTGAAGTCTTCTAGCCTGGCAGGAAGTTAC
  ACTACAGGACTATGTGTCTGCATTGTGGCCGTGCTCAGGCGGTACCATAGTTGCCTGATCCTAAATCCTG
  ATCAGACAGCCCAGGTGTTTGAAGGGTTGTGTGGTGTGGTGAAGCACGTTGTCAACCCCTCAGAATGTTC
  TTCCCCAGAGAGATGTATCTTGGCCTACCTCTATGACCTCTACGTATCCTGCAGCCACCTCAGAAGTAAA
  TTTGGAGACCTTTTTAGTAGTGCCTGTTCAAAAGTAAAGCAGACCATATACAATAATGTGATGCCTGCAA
  ATTCCAACTTACGCTGGGATCCAGACTTTATGATGGATTTTATTGAGAACCCCTCGGCAAGAAGCATCAA
  TTATTCAATGCTGGGCAAGATCCTCAATGACAATGCTGCCAACCGGTACAGCTTTGTGTGCAACACCCTC
  ATGAATGTGTGCATGGGACACCAGGATGCCGGCCGGATAAATGACATAGCCAATTTCTCCTCTGAGCTAA
  CTGCCTGCTGTACAGTTCTGAGTTCGGAGTGGCTGGGGGTTCTGAAGGCTCTTTGTTGTTCTTCAAATCA
  TGTGTGGGGATTTAATGATGTCCTCTGCACGGTGGATGTGAGTGACCTTTCATTCCATGATTCATTAGCT
  ACTTTCATTGCTATCCTGATAGCACGACAGTGTTTCTCCCTGGAAGATGTCGTGCAACACGTCGCACTTC
  CCTCTCTCCTAGCAGCAGCTTGTGGAGACGCGGATGCAGAGCCCGGGGCAAGGATGACGTGCCGCCTCCT
  GCTTCACCTCTTCAGAGCCCCCCAGGCCTGCTTTTTCCCTCAGGGAACAGGCAAACCTTTCCCCGGAATA
  AGATCATCTTGTGATAGACACCTCTTAGCTGCAGCTCACAACAGCATAGAAGTGGGAGCAGTTTTTGCTG
  TCTTAAAGGCAATTATGATGCTTGGCGATGCCAAAATTGGCAGTAACAATGTCAACACGATGAAGAATGA
  AGACTTCGGCATGAGGGGTTTACGACGTGATGGGAATGCTGAAGATGCCTGGGCCACCTCGCAGAACTCA
  AAATCGTATGGGAAAAGTATTTCCATAGAAACTGCCAATCTCAGAGAATATGCTAGATACGTACTGAGGA
  CTATCTGCCAGCAGGAATGGGTGGGAGAACACTGCTTAAAAGAACCAGAACGACTGTGTACAGACAAAGA
  ACTGATACTGGACCCCGTGCTTTCAAACATGCAAGCACAGAAATTGTTGCAGCTTATCTGCTATCCTCAT
  GGCATTAAGGAGTGTACAGAGGGGGACAACCTGCAAAGACAGCACATTAAGCGCATTCTTCAGAATCTTG
  AGCAGTGGACGTTGAGACAGTCCTGGCTGGAGCTCCAGCTCATGATCAAACAGTGCTTGAAGGACCCTAG
  CTCTGGTTCTGTGGCCGAAATGAATAATTTGCTGGACAATATCGCAAAGGCAACGATAGAAGTGTTCCAG
  CAATCTGCAGACCTAAATAATAATGCCTCCAATTCTGGCATGAGCCTCTTCAACCCAAACACTATTGGAA
  GTGTTGATCCAAGTAGCACAAGACAGAATGGAATAAAGACATTCTTAAGTTCCTCTGAACGCAGGGGTGT
  GTGGCTGGTGGCCCCCCTCATCGCCAGGCTGCCAACATCTGTGCAGGGAAGAGTGTTGAAAGCCGCTGGG
  GAAGAGCTGGAGAAGGGGCAGCACCTGGGTTCCTCTTCAAAGAAGGAAAGAGATAGGCAGAAGCAGAAAA
  GTATGTCTCTTTTGAGCCAGCAGCCTTTCCTCTCATTGGTCCTTACCTGCCTTAAGGGGCAAGATGAGCA
  ACGGGAAGGCCTTCTAACATCTCTGCAGAATCAAGTCAATCAGATTTTAAGTAACTGGAGGGAAGAAAGA
  TACCAAGATGATACGAAAGCCCGCCAGATGATGCATGAGGCCCTACAGCTCCGCCTGAATCTGGTAGGAG
  GAATGTTTGACACGGTACAGCGGAGCACGCAGGGGACCACCGACTGGGCCCTCCTACTCCTCCAGATAAT
  TACTTCGGGAACTGTTGACATGCACACTAACAATGAATTATTCACAACGGTTCTTGACATGCTGGGTGTT
  TTAATCAATGGAACCCTGGCTTCTGACCTATCAAGTGCTTCCCCCGGGGGATCAGAAGAGAACAAACGTG
  CATACATGAACTTAGTAAAGAAACTGAAAAAAGAACTAGGAGACAAGAGGTCAGAAAGCATTGACAAAGT
  TCGACAGTTGCTGCCTTTGCCAAAGCAGACCTGCGATGTCATCACGTGTGAACCCATGGGTTCTTTGATC
  GATACAAAGGGAAACAAAATTGCTGGATTTGACTCTATTGATAAAAAACAGGGTCTCCAGGTCTCTACGA
  AGCAGAAAGTGTCCCCATGGGACCTGTTTGAGGGTCAGAAGAACCCAGCCCCGCTGTCCTGGGCCTGGTT
  CGGCACTGTCCGTGTGGACCGGAAGGTGATCAAGTATGAGGAGCAGCAGCACTTCCTTCTGTACCACACG
  CACACCATGCCCAAGCCCCGAAGCTACTACCTCGAGCCGCTGCCCCTACCTCCTGAGGAGGAAGAGGAAG
  AACTCACGTCTCCAGTTTCTCAGGAACCAGAAAGGAAATCAGCTGAGCTGTCAGATCAAGGAAAAGCTAC
  AGCTGATGAAGAGAAGAAGACAAAGGGAAGGAAGCGTAAGACAAAATCGAGCTCACGCATCGATGAGTAT
  CAACAGACCAACTTATACCGAGTGCCTCCCAATTACTCCCCCATGTCCTCCCAAATGACGCATCATCCGC
  AACCTGCACTGTGGGGTTACAACCTGGTGAGCCAGCCTCAGCAGCCTAGCTTTTTCCTCCAGAATCCGTC
  TCTGAATCCAGGTGGCTCCCGATTGGACCCTGCGGGCTCCTTTGTCCCAACCAATACCAAGCAAGCTTTG
  TCCAACATGCTGCAGAGACGCTCAGGCGCCATGCTGCAGCCACCCTCCCTTCATGCAGTCACGTCTCAGC
  AACAGCTGCTACAGATGAAGCTTCTGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
  GCGGCTTCTCAGGCAGGCCCAGACTCGACCTTTCCAACAGGGCCAGCCAGGGGACCAGGCTGCTCTCTTT
  ACTGCACAAGCACGGCCCTCCCCTCAGCTCCCTCAGTATCCAGGGCTGCAACAAGCACAGACCATGCCCC
  AGGGCTACACAATGTATGGAACACAGATGCCTTTGCAACAGGCAGCTCAGCAGCAGCCTGGTGGCGTGGT
  CCTTTCCCCCAGCTACAACTCCAGGGCCTATCCGGCTGCACATTCCAGCCCAGCGCTGATGGAAAGACTC
  CGGCAGCTTCAGCAGCAGCCAAGTGGCTACGTGCAGCAGCAGGCCTCCCCGTACCTGCAGCCCGTAGCTG
  GCTCTCAGAGACTGAACCACCAGGCTCTACAGCAGAGCCCTCTGGTGGGCGGGGGAATTGACGCTGTGCT
  GACTCCCGCACATCCCAACCTCCCCTCGGTGCCCCTGCCTCAGGACCCAATGAGGCCCAGACAGCAGCAA
  GTTCGACAGCAGCAGAGACTCCTCCAGATGCAGCAGCCCCAGCAGGCCCCCCAGCCCCAGCAGCCCTCAC
  AGACCCAGAGTCAGGCCCTTGGTCTCCAAGCAATGCAGCCCCAGCAGCCTTTGTTCCCCAGGCAAGGCTT
  GCAGCAGACCCAGCAACAGCAACAGACAGCCGCCCTGGTGCGACAGCTCCAAAAGCAGCTTTCTAGTAAC
  CAGCCACAGCAAGGAGTGACTCCCTGTGCACACCCTTCACACTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177855
• Insert Size: 6558 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177855.3, NP_808523.2
• RefSeq Size: 10275 bp
• RefSeq ORF: 6558 bp
• Locus ID: 329650
• UniProt ID: Q8BQM9
• Cytogenetics: 3 D
• Gene Summary: May be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]