Dock10 (NM_175291) Mouse Untagged Clone

CAT#: MC225216

Dock10 (untagged) - Mouse dedicator of cytokinesis 10 (Dock10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dock10
• Synonyms: 9330153B10Rik; A630054M16Rik; Jr4; Jr5; mKIAA0694; R75174; ZIZ3; Zizimin3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225216 representing NM_175291
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGAGCGGACCCGCAGGTTCACCCGGAGCCTGCTGAGACCGGGACAGGCGGCCGAGCTCCGGC
  ACAGCGCCGCGTCTGCCGCCGCGGTGGCCGTCAGCAGCCGTCAGCAGCAGCGGCAGGAAAAGCCCAGACT
  CTTGGATCCTTTGGATTATGAAACCGTCATTGAAGAACTTGAAAAGACCTATCGGGATGATCCTCTTCAG
  GACCTTCTGTTCTTCCCCAGTGATGACTTCTCAACAGCCACGGTTTCTTGGGATATCCGAACACTCTATT
  CGACGGTACCAGAAGAGGCAGAGCACAGGGCAGAAAGTCTGCTGGTGAAAGAGGCTTGTAAATTCTACAG
  TTCCCAGTGGTACGTGGTAAACTACAAATATGAACAATATTCTGGAGATATTCGACAGTTGCCCCGAGCA
  GAACACAAGCCAGAGAAACTTCCTTCACATTCCTTTGAGGTTGACCATGAAGATGCTGATAAGGATGAAG
  ATACCACATCCCACTCATCATCCAAGGGCGGTGGGGGAGCTGGAGGTACTGGTGTGTTCAAGTCTGGCTG
  GCTTTACAAGGGGAATTTTAACAGCACTGTGAACAATACTGTCACTGTTCGGTCATTCAAAAAGCGCTAC
  TTCCAGCTGACTCAGTTGCCTGATAACTCCTACATTATGAACTTTTACAAAGATGAAAAGATATCCAAAG
  AGCCCAAGGGCTGCATCTTTTTGGATTCCTGCACAGGTGTAGTACAGAATAACAGGCTGAGGAAATATGC
  CTTCGAGTTGAAGATGAACGACCTGACCTATTTTGTGCTGGCTGCTGAAACAGAGTCAGATATGGACGAA
  TGGATCCACACACTTAACCGTATCCTGCAGATCAGTCCCGAGGGCCCACTCCAGGGAAGGAAAAGTGCAG
  AGCTTGCTGAGCTGGGGCTAGATCCACTGGATAACTGCGTGACTTGTGAATGTACACTGGAGGAAACGGA
  TTCTTCAGAGAACAGCCTACATCCTGACTTTGCAAAATACCTCACAGAAACCGAAGACACTGTCAAAACA
  ACACGAAACATGGGCAGACTCAATCTGTTCTCCCTGGACCCAGACATAGACACCCTGAAGCTTCAAAAAA
  GGGACAGTTTTGAAAATGAGTTGATGATCAAACCCTTTGAAGAGAAAGCTGCCAAAAGAATCATGATCAT
  CTGTAGAGCTCTCAACTTCAATCTTCAGGGATGTGTTACAGAGAATGAATATGACCCTGTAACCAATATT
  GAGCCCTTTTTTGTGAGCGTGGCACTTTACGACCTCAGAGATAACAGGAAGATCTCTGCGGACTTCCACG
  TGGACCTAAATCACCCTGCTGTCCGGCAGATGCTCTCGGGGACCCCACCGGCCTTGGAAAACGGCAATAT
  TGACACCGGTACACCAAGACAATCGGAAGAACCTCACATAAAGGGCCTTCCAGAGGAATGGTTAAAATTC
  CCAAAGCAGGCTGTCTTCTCTGTGAGTGATCCACACTCTGAAATTGTTTTGGTGGCCAAAGTCGAAAAAG
  TCTTAATGGGAAACATCGGGAGTGGTGCTGAACCTTATATCAAGAATCCAGACTCCAACAAGTTTGCACA
  AAAGATTCTAAAATCCAATAGGCAGTTCTGCAGCAAATTGGGGAAATATCGGATGCCGTTTGCTTGGGCA
  GTGAGATCTGTATTTAAGGACAACCAGGGGAACGTGGACAGGGACTCACGGTTTTCACCACTGTATAGAC
  AAGAAAGCAGCAAGATGTCGTCTGAGGACCTCCTAAAACTGGTGTCAGACTACAGAAGAGCTGACCGGAT
  AAGCAAAATGCAGAGCATCCCTGGAAGCCTGGATATTGCTGTGGACAACATTCCCTTGGAGCACCCAAAC
  TGTGTAACATCGTCCTTTATCCCTGTCAAGCCCTTCAACGTGTCGGCTCAGTCAGAACCTACAGTGGAGG
  TGGAAGAATTTATTTATGACTCAACAAAGTATTGCCGTCCATACCGAGTATACAAAAATCAAATTTATGT
  ATACCCCAAACACCTCAAATACGATAGCCAGAAATGCTTCAACAAGGCACGGAATATAACTGTGTGCATT
  GAATTCAAAAATTCAGATGACGATGGAGCCAAGCCCATGAAGTGTATTTATGGGAAACCTGGAGGGCCCC
  TCTTCACTTCCTCTGCCTACACGGCTGTGCTACACCATTCTCAAAATCCAGATTTCTCTGATGAGGTGAA
  AATTGAACTACCAACACAACTCCATGGAAAACATCACCTTTTGTTCTCCTTTTACCACATCACATGTGAC
  ATCAATGCCAAAGCCAATGCCAAAAAGAAAGAGGCTTTGGAGACATCAGTGGGCTATGCATGGCTTCCTC
  TGATGAAACATGATCAAATAGCTTCTCAGGAGTACAACATCCCAATAGCAACGACCCTGCCTCCTAATTA
  TTTAAGCATTCAAGATCCTACAAGTGCAAAGCATGGTGGAAGTGACATTAAATGGGTCGATGGTGGCAAA
  CCGCTTTTCAAAGTGTCCACATTTGTTGTATCAACAGTGAACACTCAGGACCCACATGTAAATGCATTTT
  TCCGTCAGTGCCAAAAAAGAGAAAAAGACATGTCTCAGTCACCTACCTCCAGCTTTGTCCGTGCCTGTAA
  GAACTTACTAAATGTGGACAAGATCCACTCCATCATGAGTTTCCTGCCTATCATCTTGAACCAGCTCTTC
  AAAATTCTAGTGCAGAACGAGGAAGATGAAATTACTGCGACCGTCACCAGGGTTCTGGCTGACATTGTGG
  CCAAGTGTCATGAGGAGCAACTAGACCATTCTGTCCAGTCATACATTAAGTTTGTATTCAAGACCAAATC
  CTACAAAGAAAGAACAATACATGAGGAACTGGCTAAAAATTTGAGTGATCTTTTGAAGTCCAATGACTCA
  ACGATAGTCAAGCATGTTCTAGAGCATTCTTGGTTCTTCTTTGCCATTATTCTAAAATCAATGGCACAGC
  ACTTGATTGACACAAACAAAATTCAGCTTCCCAGAGCTCAAAGATTCCCCGAGTCTTACCAAAGCGAACT
  AGACAACTTGGTGATGGGCCTGTGTGACCACGTGATTTGGAAATGCAAGGAAGCCCCTGAGGAAACCAAA
  AGAGCAAACCACAGCGTTGCCAGATTCCTTAAGCGCTGCTTTACATTTATGGACCGGGGATTCGTGTTTA
  AGATGGTGAACAATTACATCAGCATGTTCTCTTCTGGTGAGTTTAAGACTTTATGCCAGTATAAGTTTGA
  TTTCCTTCAGGAAGTTTGTCAACATGAGCACTTTATCCCTTTGTGTCTCCCCATAAGATCTGCAAACATT
  CCAGATCCCTTGACACCTTCAGAATCAATCCGAGAGTTACATGCCTCAGATATGCCTGAGTACTCAGTCA
  CAAATGAATTTTGCCGCAAACACTTCTTAATTGGAATTCTTCTCCGAGAAGTTGGCTTTGCCCTGCAGGA
  GGACCAAGACATCAGGCACTTAGCTTTAGCTGTCCTAAAAAATCTAATGGCTAAGCATTCTTTCGATGAT
  CGATACAGGGAACCTAGGAAGCAGGCACAGATAGCGAGTCTGTACATGCCGCTCTATGGTATGCTCCTGG
  ACAATATGCCAAGAATCTACCTGAAGGACCTGTATCCTTTCACCGTGAACACATCCAATCAGGGATCTAG
  AGATGACCTCAGCACTAATGGAGGATTCCAGACTCAGACGTCCATGAAACATGCAACTTCTGTGGATACA
  TCATTTTCCAAAGATGTTTTAAATTCCATAGCAGCATTTTCATCAATAGCTATTTCTACAGTGAACCATG
  CAGATTCCAGAGCGTCCTTAGCGAGCCTCGACTCCAACCCAAGTACCACAGAGAAGAGCAGTGAGAAGAC
  AGACAACTGTGAAAAGATCCCAAGGCCCTTGTCTTTGATTGGGTCAACGCTTCGGTTTGACAAATTAGAT
  CAAGCAGAAACCAGGAGTCTTCTTATGTGTTTTCTTCACATTATGAAGACCATTTCAGATGAGACTCTGA
  TTGCCTACTGGCAGAGAGCACCCAGTCCAGAGGTGTCAGACTTCTTCAGCATCTTGGACGTTTGTCTTCA
  GAATTTTAGATACCTAGGGAAACGCAATATAATAAGGAAAATCGCTGCAGCGTTTAAGTTTGTGCAGTCA
  ACCCAGAACAATGGGACTCTGAAGGGATCCAATCCTTCCTGCCAGACATCAGGTCTCTTGTCACAATGGA
  TGCACACGACTTCTGGCCACGAGGGACATAAGCAGCACAGGTCTCAGACTTTACCTATAATCCGAGGCAA
  AAATGCACTTTCCAACCCCAAACTTTTACAGATGTTGGACAACAGCATGAACAGCAATTCCAATGAAATA
  GACATTGTCCACCATGTTGACACAGAGGCCAACATAGCCACCGAGGTCTGCCTCACTATTCTGGACCTGC
  TGTCTCTCTTTACCCAGGTCCACCAGAGACAGCTCCAACAATCCGACTGTCAAAATTCACTCATGAAAAG
  GGTCTTCGATACTTACATGCTGTTTTTCCAAGTCAACCAGTCAGCCTCAGCCCTGAAACACGTGTTTGCT
  TCTTTAAGACTGTTTGTGTGCAAGTTTCCGTCAGCATTTTTCCAAGGGCCTGCTGACCTCTGTGGCTCAT
  TCTGCTATGAAGTCCTCAAATGCTGTAACCACAGGTCAAGGTTGACTCAGATGGAAGCTTCAGCACTTCT
  ATACTTCTTCATGAGGAAGAACTTTGAGTTTAACAAGCAGAAGTCAATTGTCCGGTCTCACTTACAACTC
  ATCAAAGCAGTGAGCCAGTTAATAGCTGATGCAGGGATCGGAGGGTCTCGCTTTCAACACTCCCTTGCAA
  TCACGAACAACTTTGCCAATGGAGATAAACAGATGAAAAACAGCAATTTCCCAGCAGAGGTGAAAGATCT
  GACTAAACGTATAAGGACTGTTTTGATGGCCACAGCCCAGATGAAGGAGCATGAGAAGGACCCAGAGATG
  CTGGTGGACCTTCAATACAGCCTAGCAAACTCCTATGCAAGTACCCCGGAGTTACGGAGGACCTGGCTGG
  AAAGCATGGCCAAGATTCATGCAAGAAATGGAGACCTGTCTGAGGCTGCGATGTGTTACATCCATATAGC
  TGCACTTATTGCAGAATACCTGAAGCGCAAGGGTTACTGGAAAATGGAAAAGATTTGCACACCACCCCTG
  CTTCCAGAAGACACCCAACCCTGTGATAGCAACTTATTACTAACAACTCCAGGCGGAGGAAGCATGTTCT
  CTATGGGATGGCCAGCCTTTCTGAGCATCACCCCAAACATTAAAGAAGAAGGAGCAATGAAAGAGGATTC
  TGGAATGCAAGACACCCCGTACAATGAGAACATCCTAGTGGAACAGCTGTATATGTGTGTGGAGTTCCTT
  TGGAAGTCTGAACGATACGAACTCATCGCTGATGTCAATAAGCCCATCATCGCTGTCTTTGAAAAGCAAC
  GAGACTTCAAAAAATTATCAGATCTCTATTATGACATCCACCGGTCCTATCTGAAAGTGGCAGAGGTGGT
  GAATTCGGAGAAGCGATTGTTTGGTCGTTACTATAGAGTGGCGTTTTATGGGCAGGGATTCTTTGAGGAG
  GAGGAAGGTAAAGAGTATATCTACAAAGAGCCTAAGCTGACAGGGCTCTCGGAGATCTCCCAAAGGCTTC
  TCAAGCTCTATGCAGACAAATTTGGAGCAGACAATGTGAAAATAATTCAAGATTCCAACAAGGTAAACCC
  CAAGGATCTGGACCCCAAATATGCCTATATTCAGGTGACCTATGTCACACCATTCTTTGAAGAAAAGGAA
  ATCGAGGACCGAAAGACAGACTTTGAAATGCATCACAACATCAATCGCTTTGTCTTTGAGACACCCTTCA
  CTCTGTCAGGCAAGAAGCACGGAGGAGTGGCTGAGCAGTGCAAGCGGAGGACAGTCCTGACCACAAGCCA
  CTTGTTCCCCTACGTAAAGAAGAGGATCCAGGTCATCAGCCAATCAAGCACAGAGCTGAATCCTATCGAG
  GTGGCAATTGATGAGATGTCCAGGAAGGTCTCTGAGCTTAATCAGCTGTGCACCACAGAGGAGGTGGATA
  TGATCCGCCTACAGCTCAAACTCCAGGGCAGTGTCAGCGTGAAGGTCAATGCTGGGCCAATGGCTTATGC
  TCGAGCCTTTCTTGAAGAAACTAATGCAAAGAAGTATCCTGACAACCAAGTTAAGCTACTAAAGGAAATA
  TTCAGGCAATTTGCAGATGCGTGTGGGCAGGCTCTTGATGTGAATGAGCGTCTCATCAAGGAAGACCAGC
  TGGAGTACCAGGAAGAACTGAGGTCCCATTATAAGGACATGCTCAGTGAACTGTCTGCCATCATGAATGA
  GCAGATTACGGGCAGGGACGACCCAGCAAAGTGCGGAGTGGAGCGACCCTACACCACACGTGTAACTAGC
  AAGGGGACCGCGGCTGTACCTGTGGTCTCCATCTCATCCAGTGCGGAGGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175291
• Insert Size: 6564 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175291.4, NP_780500.2
• RefSeq Size: 7308 bp
• RefSeq ORF: 6564 bp
• Locus ID: 210293
• UniProt ID: Q8BZN6
• Cytogenetics: 1 C5
• Gene Summary: Guanine nucleotide-exchange factor (GEF) that activates CDC42 and RAC1 by exchanging bound GDP for free GTP. Essential for dendritic spine morphogenesis in Purkinje cells and in hippocampal neurons, via a CDC42-mediated pathway (PubMed:25851601). Sustains B-cell lymphopoiesis in secondary lymphoid tissues and regulates FCER2/CD23 expression (PubMed:27502165).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1, alternatively referred to as DOCK10.1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.