F5 (NM_007976) Mouse Untagged Clone

CAT#: MC225213

F5 (untagged) - Mouse coagulation factor V (F5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: F5
• Synonyms: AI173222; Cf-5; Cf5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225213 representing NM_007976
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTAGTCTGCCCGTGCTTCTTCCTCCTGGTGGTTCTGGGAACCCGCTGGGCGGGCTGGGGCAGCC
  ACCAGGCAGAGGCCGCGCAACTAAGGCAGTTCTATGTGGCAGCTCAGGGGATCCTCTGGAACTATCATCC
  TGAGCCCACAGATCCAAGTTTGAATTCTATACCTTCCTTCAAGAAAATTGTCTACAGAGAGTATGAACAG
  TATTTTAAGAAAGAAAAGCCACGATCTAGCAACTCAGGACTTCTTGGACCTACTTTATACGCTGAAGTTG
  GGGACGTCATTAAAGTTCACTTTAGAAACAAAGCAGACAAACCACTAAGCATCCATCCTCAAGGGATTAA
  ATACAGTAAATTTTCAGAAGGGGCTTCTTACGCAGACCACACGTTCCCTGCCGAGAGGAAGGATGATGCC
  GTGGCTCCTGGAGAAGAATACACCTATGAATGGATCGTCAGTGAGGACAGCGGGCCCACACCTGATGACC
  CACCATGCCTCACCCACATCTACTATTCCTATGAAAACCTGACCCAGGATTTCAACTCGGGTCTGATTGG
  GCCTCTGCTTATCTGCAAGAAAGGCACCCTGACCGAGGATGGGACTCAGAAGATGTTTGACAAGCAGCAT
  GTGCTCCTATTTGCTGTGTTTGATGAAAGCAAGAGCCGGAGCCAGTCACCATCCCTAATGTACACAATTA
  ATGGCTTTGTGAATAAGACGATGCCAGATATAACAGTCTGTGCCCATGACCACGTCAGCTGGCATCTGAT
  CGGGATGAGCTCGGGGCCAGAATTGTTTTCTATTCACTTCAACGGCCAAGTCCTAGAGCAGAACCAGCAT
  AAAGTGTCCACCGTCACCCTGGTCAGCGCAACATCTACGACTGCAAACATGACTATGAGCCCAGAAGGAA
  GATGGATTGTTTCTTCTCTCATCCCAAAGCATTATCAAGCTGGGATGCAGGCTTACATTGACATTAAAAA
  CTGCCCAAAGAAAACGAGGAGCCCCAAGACCCTCACTCGGGAGCAGAGGCGGTACATGAAGAGATGGGAG
  TATTTCATAGCCGCAGAGGAGGTCATTTGGAACTATGCACCCGTGATACCTGCGAATATGGACAAAATTT
  ACAGGTCTCAGCACTTGGATAATTTCTCAAACCAAATTGGAAAACATTACAAGAAAGTTATCTACAGGCA
  ATATGAAGAAGAGACCTTCACCAAACGCACTGACAACCCCAGCATCAAACAAAGTGGGATTCTGGGCCCT
  GTTATCAGAGCCCAGGTCAGAGACACACTCAAGATCGTGTTCAAAAATATGGCGAGCCGACCCTACAGCA
  TTTACCCTCACGGGGTGACCTTCTCTCCTTACGAAGATGGAATCAATTCTTCCTCCACCTCAGGCAGTCA
  CACCACGATCAGACCAGTTCAACCGGGGGAAACCTTCACTTACAAATGGAACATTCTAGAGTTTGATGAA
  CCCACGGAAAACGATGCCCAGTGCCTAACAAGGCCATACTACAGTGATGTGGACGTTACAAGGGATATTG
  CCTCTGGGCTGATAGGGCTGCTTCTAATTTGTAAGAGCAGGTCCCTGGACCAGAGGGGTGTACAGAGGGT
  GGCAGACATCGAGCAGCAGGCCGTGTTTGCTGTGTTTGACGAGAACAAGAGCTGGTACATTGAGGACAAC
  ATCAACAAGTTCTGTGAGAATCCTGATGAGGTGAAGCGTGATGATCCCAAGTTTTACGAATCAAACATCA
  TGAGCACTATCAACGGCTACGTGCCCGAGAGCATTTCCACTCTGGGATTCTGTTTTGATGACACTGTCCA
  GTGGCACTTCTGCAGTGTGGGAACTCATGATGATATTTTGACCATCCACTTCACTGGGCACTCGTTCATC
  TATGGGAGGAGGCACGAGGACACCTTGACCCTGTTCCCCATGCGTGGTGAATCTGTGACAGTTACAATGG
  ATAATGTTGGAACTTGGATGTTGACCACCATGAATTCCAATCCAAAACGCAGAAACCTAAGACTGAGATT
  CAGAGATGTTAAGTGTAATCGGGATTATGACAATGAGGACTCATATGAGATTTATGAACCTCCTGCACCT
  ACATCCATGACAACTCGGAGAATTCATGATTCCTTAGAAAATGAATTTGGCATAGACAACGAAGATGATG
  ATTACCAGTACTTACTGGCGTCATCATTAGGAATTAGGTCATTCAAAAACTCATCATTGAATCCAGAGGA
  AAATGAGTTCAATCTCACTGCTCTCGCTCTGGAGAACAGCTCTGAGTTCATATCTCCAAGCACAGACAGA
  GTTGTTGACTCAAACTCTTCACGAATCCTTAGTAAAATCATCAATAATAACCTCAAAGACTTTCAAAGAA
  CACTTCCTGGCTCAGGAGCCACCGTGGCTGGTACCCTCCTTAGAAACCTCATTGGCTTAGATGAGAACTT
  CGTCCTCAACTCTTCTACAGAACATCGTTCCAGCTCATATCATGAAAATGATATGGAAAATCCACAGTCA
  AACATCACAATGGTATACCTACTTCCTCTTGGTCCAAAAGGATCTGGGAATCGAGAACAAGATAAACCTA
  AAACCATCAAGACAGGAAGACCCCACATGATGAAGCACAGGTTCTCCTGGATGAAAGCGCCAGCTGGTAA
  AACTGGGAGGCATTCAAACCCAAAGAATTCGTATTCTGGAATGAAGTCTGAGGAGGACATTCCTAGCGAG
  TTGATACCCTTAAAGCAAAAGATCACTTCCAAATTTCTGAATAGACGATGGCGTGTGGCTTCTGAAAAGG
  GTAGTTATGAAATAATAGCAGCAAATGGTGAAGACACAGATGTGGATAAGCTGACCAACAGTCCTCAAAA
  TCAGAATATCACAGTACCTCGGGGAGAGAGCACCTCTCACACAAACACAACAAGAAAGCCAAGTGACCTC
  CCAACATTTTCTGGAGTTGGACATAAATCTCCACATGTAAGACAGGAGGAAGAAAACAGTGGTTTTCAGA
  AAAGACAGTTATTCATCAGGACACGGAAGAAGAAGAAAAATAAGAAGCTTGCACTACACAGTCCTCTATC
  TCCAAGGGGCTTTGACCCTTTGAGAGGACATAACCATTCCCCATTTCCAGACAGGAGACTACTTAATCAC
  TCACTGTTACTCCACAAGTCCAATGAAACAGCTCTTTCTCCAGACCTGAACCAGACCTCTCCTTCAATGA
  GTACGGACAGGTCACTTCCTGACTATAATCAGTACTCGAAAAATGACACTGAGCAGATGAGCTCTTCTTT
  AGATCTTTATCAGTCAGTGCCCGCAGAGGAACACTCTCCAACATTTCCTGCCCAAGATCCTGATCAAACA
  CACTCTACCACAGATCCTAGCTACAGATCCTCTCCGCCAGAGCTCAGCCAGGGGCTTGATTATGACCTAA
  GTCATGACTTTTACCCTGATGACATTGGTCTAACATCTTTCTTTCCAGACCAAAGTCAAAAGTCATCTTT
  CTCTTCAGATGATGACCAAGCAATCCCTTCCTCAGACTTAAGCCTCTTTACCATCTCTCCAGAATTGGAT
  CAGACAATTATTTACCCAGACCTGGATCAGTTGCTCCTTTCTCCAGAAGACAATCAGAAGACCTCCTCCC
  CAGACCTGGGCCAGGTGCCCCTTTCTCCAGATGACAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGT
  GTCCCTTTCTCCAGATGATAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCTAGAT
  GACAACCAGAAGACGACCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCCAGATGACAACCAGATGATCA
  CCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTTCTGATAACCAGAAGACCTCTTCCCCAGATCTGGGTCA
  GGTGCCTCTTTTTCCTGAAGACAACCAGAATTACTTCCTAGACCTGAGTCAGGTACCTCTCTCCTCAGAC
  CAAAACCAGGAGACCTCCTCCACAGACCTACTGACTCTCTCTCCTGATTTTGGTCAGACAGTCCTTTCCC
  CAGACTTGGATCAGCTGCCACTCCCTTCAGACAATAGTCAGGTGACCGTTTCCCCAGACCTCAGCCTCTT
  GACCCTCTCACCAGATTTTAATGAGATAATCCTAGCCCCAGACCTTGGTCAAGTGACCCTCTCTCCAGAC
  CTCATCCAGACAAACCCTGCTCTTAATCATGGACACAAAGCATCCTCTGCAGACCCTGATCAAGCATCCT
  ACCCTCCAGATTCTGGTCAGGCTTCATCGCTTCCAGAACTGAATCGGACTCTTCCTCATCCAGATCTCAC
  TCACATACCACCTCCTTCACCATCTCCCACACTCAATAACACTTCTTTGTCAAGGAAATTTAACCCTCTT
  GTTGTAGTAGGTCTCAGTAGAGTAGATGGAGACGACGTTGAGATTGTTCCAAGTGAGGAGCCAGAGAGAA
  TAGATGAAGATTATGCCGAGGATGACTTTGTAACCTATAATGACCCCTACAGAACAGACACTAGGACAGA
  TGTCAATTCCTCCAGAAATCCTGACACTATCGCAGCATGGTACCTCCGAGGCCACGGTGGACACAAAAAA
  TTCTACTATATTGCAGCTGAAGAAATAACCTGGAATTACGCAGAGTTTGCACAAAGTGAAATGGACCATG
  AAGACACAGGCCACACTCCAAAGGACACCACATACAAGAAAGTCGTTTTCAGAAAATACCTTGATAGCAC
  GTTTACAAGTCGTGATCCTCGGGCAGAATATGAGGAGCACCTTGGCATTCTCGGTCCTGTGATCCGGGCT
  GAAGTGGATGATGTGATCCAAGTTCGATTTAAAAATTTGGCATCCAGACCGTATTCTCTTCATGCTCACG
  GACTTTCCTATGAAAAATCCTCAGAGGGGAAGACTTATGAAGATGAATCTCCTGAATGGTTTCAGGAAGA
  TGATGCTGTCCAGCCCAATAGCAGTTACACCTATGTATGGCATGCCACCAAGCGCTCAGGGCCAGAGAAC
  CCTGGTTCTGCCTGCCGGGCTTGGGCCTACTATTCTGCAGTGAATGTGGAGAGGGACATCCACTCAGGCT
  TGATCGGCCCCCTTCTGATCTGCCGGAAAGGAACACTTCACATGGAGCGCAACCTGCCTATGGACATGAG
  AGAGTTTGTCTTACTCTTCATGGTCTTTGATGAGAAGAAGAGCTGGTACTATGAAAAGTCCAAGGGGTCA
  CGGAGAATTGAATCCCCAGAAGAGAAAAATGCCCACAAGTTTTACGCAATTAATGGGATGATCTACAACC
  TGCCCGGCCTGAGAATGTACGAGCAAGAGTGGGTGAGGCTACACCTGCTGAACATGGGCGGCTCCCGAGA
  TATTCACGTGGTTCACTTCCATGGCCAGACCCTGCTGGATAATAGGACCAAACAGCACCAGTTAGGCGTC
  TGGCCCCTTCTGCCTGGTTCATTTAAAACTCTTGAAATGAAGGCATCCAAGCCTGGCTGGTGGCTCCTAG
  ACACAGAGGTTGGAGAAAACCAGGTAGCTGGCATGCAAACGCCATTTCTCATCATAGACAAAGAGTGTAA
  GATGCCAATGGGACTAAGCACTGGTGTCATATCTGATTCACAGATCAAGGCTTCGGAATATCTGACTTAT
  TGGGAGCCCAGATTAGCACGATTAAACAATGCTGGTTCATACAATGCTTGGAGTATAGAAAAAACTGCAT
  TAGATTTTCCCATTAAACCTTGGATCCAGGTGGACATGCAGAAGGAAGTTGTAGTCACCGGGATACAAAC
  CCAAGGTGCTAAACACTACCTAAAGTCCTGCTTTACCACGGAGTTCCAAGTGGCTTACAGCTCTGACCAA
  ACCAACTGGCAGATCTTCAGAGGGAAGAGCGGGAAGAGCGTGATGTATTTTACTGGTAATTCAGATGGCT
  CTACAATAAAAGAGAATCGACTTGACCCACCCATTGTGGCTAGATACATTAGGATACACCCAACAAAATC
  CTATAATAGACCCACCCTTCGGCTGGAGCTGCAGGGCTGTGAGGTGAACGGATGTTCCACACCACTGGGC
  CTGGAAGATGGACGGATTCAAGACAAGCAAATTACTGCATCTTCATTTAAAAAGTCGTGGTGGGGAGACT
  ACTGGGAGCCCTCCCTTGCCCGCCTGAACGCCCAGGGCCGCGTGAACGCCTGGCAAGCCAAGGCAAACAA
  CAACAAGCAGTGGTTACAAGTCGATCTGCTCAAAATCAAGAAGGTAACGGCCATCGTAACGCAGGGCTGT
  AAGTCTCTGTCCTCTGAGATGTACGTGAAGAGCTACAGCATCCAGTACAGTGACCAGGGTGTGGCATGGA
  AACCTTACCGACAGAAATCCTCCATGGTGGACAAGATTTTTGAAGGAAACAGCAATACCAAGGGGCACAT
  GAAGAACTTTTTCAACCCGCCCATTATTTCCAGATTTATCCGCATCATTCCTAAAACATGGAACCAGAGC
  ATCGCCCTTCGCCTAGAGCTCTTCGGCTGTGACATTTATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007976
• Insert Size: 6552 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007976.3, NP_032002.1
• RefSeq Size: 7433 bp
• RefSeq ORF: 6552 bp
• Locus ID: 14067
• UniProt ID: O88783
• Cytogenetics: 1 71.46 cM
• Gene Summary: This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015]