Myo7a (NM_008663) Mouse Untagged Clone

CAT#: MC225211

Myo7a (untagged) - Mouse myosin VIIA (Myo7a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myo7a
• Synonyms: Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225211 representing NM_008663
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTATTCTGCAGAAGGGGGACTATGTATGGATGGACCTGAAGTCAGGCCAGGAGTTTGATGTGCCCA
  TCGGGGCCGTGGTGAAGCTCTGCGACTCGGGCCAGATCCAGGTGGTGGATGATGAAGACAATGAACACTG
  GATATCCCCTCAGAATGCCACGCACATCAAGCCAATGCACCCCACATCGGTGCACGGCGTGGAGGACATG
  ATCCGCCTGGGGGATCTCAACGAGGCAGGCATCCTTCGAAACCTTCTCATTCGCTACCGGGACCACCTCA
  TCTATACGTACACAGGTTCCATCCTGGTGGCCGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGA
  GCACATCCGCCAGTACACCAACAAGAAGATAGGGGAGATGCCCCCCCACATCTTCGCCATTGCTGACAAC
  TGCTACTTCAACATGAAACGCAACAACCGGGACCAGTGCTGTATTATCAGCGGGGAGTCGGGAGCTGGCA
  AGACAGAGAGCACAAAGTTGATCCTGCAGTTCCTGGCAGCCATCAGTGGACAGCACTCATGGATCGAGCA
  GCAGGTGCTGGAGGCCACCCCGATCCTGGAAGCATTTGGGAACGCCAAGACCATCCGCAACGACAACTCT
  AGCCGCTTTGGCAAGTACATTGACATCCACTTTAACAAGCGTGGTGCCATCGAGGGCGCCAAAATAGAGC
  AATACCTGCTGGAGAAGTCACGTGTCTGCCGCCAGGCCCCTGACGAGAGGAACTATCACGTGTTCTACTG
  TATGCTGGAGGGCATGAATGAGGAGGAGAAGAAGAAACTGGGCCTAGGCCAGGCCGCTGACTACAACTAC
  TTGGCCATGGGTAACTGCATCACCTGTGAGGGCCGCGTGGACAGTCAGGAGTATGCCAACATCCGCTCTG
  CCATGAAGGTTCTCATGTTCACAGACACGGAGAACTGGGAGATCTCGAAGCTTCTGGCTGCCATCCTACA
  CATGGGCAATCTGCAGTATGAGGCCCGGACATTTGAGAACTTGGATGCGTGTGAAGTCCTCTTCTCCCCA
  TCGCTGGCCACGGCAGCTTCTCTGCTCGAGGTGAACCCCCCAGACCTGATGAGCTGCCTCACCAGCCGCA
  CCCTCATCACCCGTGGGGAGACGGTGTCCACCCCTCTCAGCAGGGAACAGGCGCTGGATGTGCGAGATGC
  CTTTGTCAAGGGCATCTATGGGCGGCTCTTTGTGTGGATTGTGGAGAAGATCAACGCAGCAATCTACAAG
  CCACCCCCCCTGGAAGTGAAGAACTCTCGCCGGTCCATCGGTCTCCTGGACATCTTTGGATTTGAGAACT
  TCACTGTGAACAGCTTCGAGCAGCTCTGCATTAACTTTGCCAATGAGCACCTGCAGCAATTCTTCGTGCG
  GCACGTGTTCAAGCTGGAGCAGGAGGAGTACGACCTGGAGAGCATCGACTGGTTGCACATTGAGTTCACT
  GACAACCAGGAAGCACTGGACATGATTGCCAACCGGCCTATGAACGTCATCTCCCTCATCGATGAGGAGA
  GCAAGTTCCCCAAGGGCACGGATGCCACCATGCTGCATAAGCTGAACTCACAGCACAAGCTCAATGCCAA
  CTACGTGCCACCCAAGAACAGCCACGAGACCCAGTTTGGAATCAACCACTTTGCGGGTGTTGTCTATTAT
  GAGAGTCAAGGCTTCCTGGAGAAGAACCGAGACACCCTGCATGGGGACATCATCCAGCTGGTCCACTCTT
  CCCGGAACAAGTTCATAAAGCAGATTTTCCAAGCTGACGTTGCCATGGGTGCCGAGACCAGGAAGCGCTC
  GCCTACACTCAGCAGCCAGTTCAAGCGGTCTCTGGAGCTGCTGATGCGCACACTGGGCGCCTGCCAGCCC
  TTCTTTGTGCGTTGTATCAAACCCAATGAGTTCAAGAAGCCCATGCTCTTCGACCGGCACTTGTGTGTAC
  GCCAGCTGCGATATTCGGGCATGATGGAGACAATCCGCATCCGCCACGCAGGCTACCCCATTCGCTACAG
  CTTTGTGGAGTTTGTGGAGCGCTACCGGGTACTGCTGCCTGGTGTGAAGCCAGCATACAAGCAGGGTGAC
  CTCCGAGGGACATGCCAGCGCATGGCTGAGGCTGTGCTGGGCACGCACGATGACTGGCAGATTGGCAAAA
  CCAAGATCTTTCTGAAGGACCACCATGACATGTTGCTGGAGGTGGAGCGGGACAAGGCCATCACAGACAG
  AGTCATTCTCCTCCAGAAGGTTATCCGGGGCTTCAAAGACAGGTCCAACTTCCTGAGACTGAAGAGTGCT
  GCCACACTGATCCAGAGGCACTGGCGGGGCCACCACTGTAGGAAAAACTATGAGCTGATTCGTCTTGGCT
  TCCTGCGGCTGCAGGCCCTGCACCGCTCCCGGAAGCTGCACAAGCAGTACCGCCTGGCCAGACAGCGCAT
  AATAGAGTTCCAGGCCCGCTGCCGGGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCCGTG
  ATCACCGTGCAGGCCTATGCCCGAGGCATGATTGCCCGCCGGCTACACCGCCGCCTCCGGGTTGAGTACC
  AGCGGCGCCTCGAGGCAGAGAGGATGCGTCTGGCAGAGGAGGAGAAACTCCGAAAGGAGATGAGTGCCAA
  GAAGGCCAAAGAGGAGGCTGAGCGCAAGCATCAGGAGCGCCTGGCTCAGCTAGCCCGCGAGGATGCGGAG
  CGGGAACTGAAGGAGAAGGAGGAGGCTCGGAGGAAGAAGGAACTGCTGGAGCAGATGGAGAAGGCCCGCC
  ACGAACCCATCAACCACTCAGATATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGCAGCCTGCC
  AGGCCAGGAAGGCCAGGCGCCTAGTGGCTTTGAGGACCTAGAGCGCGGACGGAGGGAGATGGTGGAAGAG
  GATGTTGACGCTGCCCTGCCCCTGCCTGATGAAGACGAGGAGGACCTTTCTGAGTACAAATTCGCCAAGT
  TTGCTGCCACCTACTTCCAGGGCACAACCACACACTCCTACACCCGGAGGCCTCTCAAGCAGCCGCTGCT
  CTACCACGACGATGAGGGTGACCAGCTGGCGGCGCTGGCTGTCTGGATCACCATCCTCCGGTTCATGGGG
  GACCTCCCAGAGCCCAAGTACCACACAGCCATGAGCGACGGCAGTGAGAAGATCCCAGTGATGACTAAGA
  TCTACGAGACCCTAGGCAAGAAGACATATAAGAGGGAGCTGCAGGCCTTGCAGGGCGAGGGCGAGACCCA
  GCTCCCTGAGGGGCAGAAGAAGACCAGTGTGAGACACAAGTTGGTACACTTGACACTGAAGAAAAAGTCC
  AAACTCACAGAAGAGGTGACCAAGAGGCTGAACGATGGGGAATCCACGGTACAGGGCAACAGCATGCTGG
  AGGATCGGCCCACCTCAAATCTAGAGAAGCTGCACTTCATCATCGGCAACGGCATCCTGCGGCCTGCGCT
  GCGGGACGAGATTTACTGCCAGATCAGTAAGCAGCTCACACACAACCCATCCAAGAGCAGCTATGCCAGG
  GGCTGGATCCTCGTGTCGCTCTGTGTGGGCTGCTTCGCCCCCTCTGAGAAGTTCGTTAAGTACCTGCGGA
  ACTTCATCCACGGAGGCCCACCTGGCTATGCTCCTTACTGTGAGGAGCGCCTGAGGAGGACCTTTGTCAA
  CGGAACTCGGACACAGCCACCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCCATCATGTTG
  CCCGTGACCTTCATGGATGGGACCACCAAGACCCTGCTAACAGATTCAGCAACTACAGCCAGGGAGCTGT
  GCAATGCTCTGGCTGACAAGATCTCACTCAAGGACCGCTTTGGCTTCTCCCTCTACATCGCTCTGTTCGA
  TAAGGTGTCCTCCCTGGGCAGCGGCAGTGACCATGTCATGGATGCCATCTCTCAGTGTGAGCAGTACGCC
  AAGGAGCAGGGTGCTCAGGAGCGCAACGCCCCATGGAGGCTCTTCTTTAGAAAGGAGGTCTTCACACCCT
  GGCACAACCCCTCGGAGGACAACGTGGCCACGAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTT
  TGGGGAGTACAGGTGTGAGAAGGAGGACGACCTGGCTGAGCTGGCTTCTCAGCAGTACTTTGTGGACTAT
  GGTTCTGAGATGATTCTGGAGCGCCTGCTGAGCCTCGTGCCCACTTACATCCCTGACCGTGAGATCACAC
  CGCTGAAGAATCTTGAGAAGTGGGCACAGCTGGCCATTGCTGCCCACAAGAAGGGAATTTATGCCCAGAG
  GAGAACTGACTCCCAGAAGGTCAAAGAGGATGTGGTCAATTATGCCCGTTTCAAGTGGCCCTTGCTCTTC
  TCCAGGTTTTACGAAGCTTACAAATTCTCAGGCCCTCCCCTCCCCAAGAGCGACGTCATCGTGGCTGTCA
  ACTGGACGGGTGTGTACTTCGTGGACGAGCAGGAGCAGGTGCTTCTGGAGCTGTCCTTCCCGGAGATCAT
  GGCTGTGTCCAGCAGTAGGGGAACAAAGATGATGGCCCCCAGCTTTACCCTGGCCACCATCAAAGGAGAT
  GAGTACACCTTCACATCCAGCAATGCTGAGGACATCCGTGACCTGGTGGTCACCTTTCTGGAGGGGCTAC
  GGAAGAGGTCTAAGTATGTGGTGGCACTGCAGGACAATCCTAACCCTGCTGGTGAGGAGTCAGGCTTCCT
  CAGCTTCGCCAAGGGAGACCTCATCATCCTTGACCATGATACTGGTGAGCAGGTCATGAACTCAGGCTGG
  GCCAACGGCATCAACGAGAGGACCAAGCAGCGCGGCGACTTCCCCACTGACTGTGTATACGTCATGCCCA
  CTGTCACCTTGCCACCAAGGGAGATTGTGGCCCTGGTCACTATGACCCCAGACCAGAGGCAGGATGTCGT
  CCGGCTCCTGCAGCTTCGCACAGCAGAGCCAGAGGTGCGCGCCAAGCCCTACACGCTAGAGGAGTTCTCC
  TACGACTACTTCAGGCCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGCGGTAAGG
  ACAGGCTGTGGAGCCACACACGAGAGCCCCTCAAGCAGGCCCTGCTCAAGAAGATCCTGGGCAGTGAAGA
  ACTCTCCCAGGAAGCCTGCATGGCCTTTGTAGCTGTGCTCAAGTACATGGGCGACTACCCATCCAAGAGG
  ATGCGATCCGTCAATGAGCTCACTGACCAGATCTTTGAGTGGGCACTCAAGGCTGAGCCCCTCAAGGATG
  AGGCCTACGTGCAGATCCTGAAGCAGCTGACTGACAATCACATCAGGTACAGCGAAGAGAGGGGCTGGGA
  ACTGCTGTGGCTGTGCACGGGCCTCTTCCCGCCCAGCAACATCCTCCTGCCTCATGTTCAGCGGTTTCTG
  CAGTCCCGCAAGCACTGTCCTCTTGCCATTGACTGCCTGCAGAGGCTCCAGAAAGCCCTGAGAAATGGCT
  CCCGGAAGTACCCTCCGCACCTGGTGGAGGTGGAGGCCATCCAACATAAGACTACCCAGATCTTCCACAA
  GGTCTACTTCCCCGATGACACGGACGAGGCTTTTGAGGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGC
  CAGAACATCGCCAGCCGGCTGCTGCTCAAGTCTTCCGAGGGATTCAGCCTTTTTGTCAAAATCGCAGATA
  AGGTCATCAGCGTCCCAGAGAATGATTTCTTCTTTGACTTTGTCCGACACCTGACAGACTGGATAAAGAA
  AGCACGGCCCATCAAGGACGGAATCGTGCCCTCACTAACCTACCAGGTGTTCTTCATGAAGAAGCTGTGG
  ACCACCACAGTGCCGGGCAAGGACCCCATGGCTGACTCCATCTTCCACTATTACCAGGAACTGCCCAAAT
  ATCTCCGAGGCTACCACAAGTGCACCCGGGAGGAGGTGCTGCAGCTGGGCGCACTCATCTACAGGGTCAA
  GTTTGAGGAGGACAAATCCTACTTCCCTAGCATCCCCAAGTTGCTGAGGGAGCTGGTACCCCAGGACCTA
  ATCCGGCAGGTCTCACCTGATGACTGGAAACGGTCTATTGTCGCCTACTTCAACAAACATGCGGGGAAGT
  CCAAGGAGGAAGCCAAGCTGGCCTTCCTCAAACTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTT
  TGAGGTGAAGCAAACTACAGAACCAAACTTCCCAGAGATTCTCTTAATTGCCATCAACAAGTACGGGGTC
  AGCCTCATCGATCCCAGAACCAAGGACATCCTGACTACTCACCCCTTCACCAAGATCTCCAACTGGAGTA
  GTGGCAACACCTACTTCCACATCACCATTGGGAACTTGGTCCGTGGGAGCAAACTGCTCTGTGAGACATC
  GCTGGGATACAAAATGGATGATCTTCTGACTTCCTACATCAGCCAGATGCTCACAGCCATGAGCAAGCAG
  AGGAACTCCAGGAGTGGAAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008663
• Insert Size: 6534 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008663.2, NP_032689.2
• RefSeq Size: 7361 bp
• RefSeq ORF: 6534 bp
• Locus ID: 17921
• UniProt ID: P97479
• Cytogenetics: 7 53.57 cM
• Gene Summary: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.