Svil (NM_153153) Mouse Untagged Clone

CAT#: MC225209

Svil (untagged) - Mouse supervillin (Svil), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Svil
• Synonyms: AU024053; B430302E16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225209 representing NM_153153
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAGAAAAGAAAGAATTGCTAGGCGCTTAGAAGGAATAGAAAATGACTCCCAGCCCATCCTGTTGC
  AGAGCTGCACAGGGCTAGTGACTCATCGGCTGCTAGAAGAAGACACACCCAGATACATGCGTGCCACAGA
  CCCTGCAAGCCCTCATATTGGGCGATCCAAAGAAGAGGAAGACACTCCTGGGTCCTCCCTAGAAAAGCAA
  ACTCCTTCTAAATACTGCATAGAAACCTCAGGCATCCATAGTTCGGGCTCCATGGACACCCACAGTCTGG
  AGTCCAAAGCGGAAAGGATCGCAAGATACAAAGCAGAGAGGAGGCGGCAGCTGGCCGAAAAGTACGGGCT
  GACCTTGGACCCAGAAGCTGACTCTGAATACTTGTCTCGATATGCCAAGTCTCGGAAGGACCCAGATGTG
  ACTGAGAGACGAGGAAAAAGTGACAAGCAAGAAGAACAGAGCAAGGATGCCAATTCCCGGCACTCCAGGA
  CAGAGTCAGGGCCCAGGACCAGCTTGGTGGCGTCCCAGGACTGTACCCCGCTGGGAAGCAATATGTCTGA
  CCAGGAGCAGCTGCTCAATGTGGAGAACCAGAGAAGAGTCCAAGATCCCCCACTAGGAGAAGATGGCTCC
  TCTGCCTTCTTTTCTGAGCGAAGCATTTCCTTCCCCGAAGTGCCCAGGTCTCCAAAGCAGATACCCAGCT
  CACCACTGCAACAGCCAGCCTCCCCAAACCACCCCGGTGACTCACCACTGCCCACAGAGGCCCGAGCCAG
  TACAGGGAAGCCCACACATGAGTGGTTTCTGCAGAGAGATTCTGAAGGCGATACACCTTCACTTATCAAC
  TGGCCTTCCAGAGTTAAAGTTAGAGAAAAATTGGTGAAAGAGGAGAGTGCTCGCAGCAGCCCTGAACTCA
  CCTCAGAGTCCTTAACTCAGAGGAGACAGCAGCCGGCACCAGCACATTTCCTGCCTATCCAGTCAGAAAG
  TTCCACCTTTGATAGGGTCACCAGCAAAGCAGTGAGCTCATTGCAGCCAAGCCAGAGTGGTGTTCTGCCA
  ACTGACCCTGTTCATGCCATCAAGCTGGTGACCATGGATACCCCAGAAAGCACATCTGAATTCAGCTGGG
  TGGGGTCAGCCACCCCAAAAGTTATAAAATCTACCACCTTGAAGATTCTAGAAGGTGGTTCAAGGGATGC
  TCCAGTACTCCATATTTGTGAGTCAAAAGCAGAAGATTGGCTCTCCCCAGAACCTCTGGAGAGGAGCCCC
  AAGTCACTTTTGACCTCAGAAGATGACAGGCTCGTGAGAGGCCACAAAGATCCCTCTGGTAATAAAGACT
  TGGACAAGGCCATCATTTGCTCCATCGATGTAGAATCTGAGAGGGAGCGGCAAGTTCAACACCTGCCCAC
  TCAGAGAACTGGCCGGAGTGAAATGCTCCTGTATGTTCAGAGTGGTCCTGTGTCCCAAGATGCCACATTG
  ACCAGTCACACAAAGGAGGCATCTCCAAAGAAGCGCAAGGTTCTGGCCCGCTCTCTGTCAGATTACACAG
  GTCCCCCTCAGCTCCAGGTCCCCAGACACAAGGATGAAGCCCCAAGCCAGGAGCTGGAGCTGCAGAGTTC
  CAGGGCAGAAGGGCCTGGTGCAGAAGCCAGCGTGCTGGACACCAGAGTCTCCGTCGCCCAGCTCCGAAAC
  ATATTTATGGAGTCAACCCGAGCCAGCAAGAAACCTGAACTCCAATCCCGGGTTGAGAGGTCAGCTGAAG
  GAATTGGCTTGCCCATGGAACGAGAGAGAGGGTCTCGGAAACCAAGACGCTATCTTTCTCCTGGTGAAAG
  TAGAAAAACTTCTGAGCGATTTAGAACTCAACCTATAACCTCAGCAGAGCGAAAGGAGTCGGATAGGTAC
  CCTTCTGGTTCAGAAATACCAGTGGTCGAGGATGAAGAAAAGGTGGATGAGCGGGCCAAGCTGAGCGTGG
  CTGCCAAGAGGCTGCTTTTCAGGGAAATGGAGAAATCCTTTGATGAACACACTGTCCCAAAGAGACATTC
  GAGAAATGCAGCTGTGGAGCAGAGGCTGCGTCGCCTGCAGGACCGTTCACACACACAGCCCATCACCACG
  GAGGAAGTGGTCATTGCAGCTACTGAACCTATCCCTGCTTCGTGTTCTGGGGTCACCCACCCTGTAACGG
  CGAGACTTCCTAGCCCCACTGTAGCTAGAAGCAGTGTGCAGCCTGCCAGGTTACAAGCATCTGCTCACCA
  AAAGGCTTTAGCCAGGGACCAGGCAAATGAGGGCAGAGAGTCTGCCGAACCAGGCGAACCTGATTCCTCC
  ACTCTGAGCTTAGCAGAGAAATTGGCCTTGTTTAACAAATTGTCCCAGCCAGTCTCCAAAGCCATTTCCA
  CCCGAAACAGAATAGATGTGCGGCAGAGGAGGATGAACGCAAGGTACCAGACGCAGCCGGTCACTCTTGG
  AGAAGTGGAGCAGGTGCAGAGTGGGAAACTCATCTCCTTCTCCCCCACTGTGAACACCTCTGTTTCTATT
  ATGGCCTCTGCGGTTGCTCCCACATATGCAGGGGACCTTCGGAAGCTATCTGTTGACAACAACACAAGTG
  CCACTGACTATAAGTCTCCTCCTGCGGAAAACTCAGACTCTCCAGTCAGAAGCATTCTGAAACCACAAGC
  TTGGCGGCCTTTGGTAGAGCACAGTGGGAGCAAAGGAATGCCGGGGGAGTCTGGAAAGACAGAAAGCAAG
  AATGCTCTGACTGTGGCTGCAGAAGACAGTGGCGTTCAAACACGAGGAGCCTTCGAGGAGGAAGAGGAGC
  CCTCCTACCCCATCCTTGGCAGAGTCAGGGAGGGTGATGGCCAGAAGGAGCCTAAGCATGTCGTCCTAAG
  GAGGGGAAGCTTAGAGCTAGGCAATCCTTCTGCTGCACACCTTGGTGATGAGCTGAAGGAAGTTTCCACT
  GCTAAAAGCAGTTTGCAAGAGAATCTAGACCTGAAGGATAAGCAGGCCTCAGAGGAGAACACAGATGTGG
  AAACTGTCATGAGGAAGTTCTCACTGAAAGAGTTTGGAGAAACGACTTCTGAGCAGACAGAGGTGGCTGC
  CAGGAAGGCCTCGGTTCAGATGGCGACCCCAGGGGCCTGGAAGCAGCAGGAGTCCTCTGAGCAACTAGCA
  GAGAAGCTCTTCAAGAACCCTTGTGCCATGTTTGCTTCTGGAGAGGTCAAGGTGCCAGTAGGCGACAGTT
  TCCTGGATTCACCCAGCAAAACCATGTCAATCAAAGAAAGGCTGGCACTGTTAAAGAAGAGTGGCGAGGA
  AGACTGGAAGAACAGGCTGATTCGGAAGCAGGAATATGGCAAAGCCACTGGCGGCCTGCACACGCAGGAG
  GTGGAGCAGTCCCTGAAGAAAAAGAGAGTCACAGAAAGTCGAGAGAGCCAGATGACGATTGAAGAGAGGA
  AGCACCTCATCACTGTGCGCGAGGAAGCGTGGAAGACGAAGGGCAGAGGCGCTGCCAATGACTCCACACA
  GTTCACCGTGGCAGGAAGAATGGTGAAGAAAGGTCTGGCATCACCCACATCCATAACTCCCATCTCATCC
  CCTCTCTGCAGTAAATCAAGGGGCACTACCCCGGTTTCCAAACCCTTGGAAGATATTGAAGCCAGACCAG
  ATATGCAGCTGGAATCAGACCTGAAGTTGGACAGACTGGAAACCTTCCTAAGGAGGCTAAATAACAAGGT
  TGCTGGGATACAGGAAACGGTTCTCACGGTCACTGGCAAATCTGTCAAGGAGGTGATGAAGTTGGATGAT
  GATGAAACATTTGCCAAATTCTACCGCAGCGTGGATCACAGTATACCCAGAAGTCCTGTGGAGCTGGAGG
  AGGACTTCGATGTCATTTTCGACCCTTATGCTCCCAAGTTGACATCTTCTGTGGCAGAGCATAAGCGCCA
  GGTCAGGCCCAAGCGCCGGGTCCAGGCTTCCAAGAACCCTCTCAAGCTGCTGGCAGCAAGAGACGACCTC
  CTTCAGGAATACACAGAGCAAAGGCTAAATGTGGCCTTCATGGAGTCCAAGCGGATGAAAGTGGAAAAGA
  TGTCATCCAACTCCAACTTCTCAGAAGTGACTCTGGCAGGCCTGGCCAGTAGGGAGAACTTCAGCAACAT
  CAACCTGAGGAGTGTCAATCTGATGGAGCAAAACTCCAACAACAGCGCGATGCCCTACAAGAAGCTCATG
  CTGTTGCAGATTAAAGGAAGAAGACATGTGCAGACAAGGCTGGTGGAGCCCCGAGCCTCATCACTCAACA
  GTGGGGACTGCTTCCTTCTGCTGTCTCCTCAGTACTGCTTCCTGTGGGTGGGAGAGTTCTCCAATGTCAT
  CGAGAAAGCAAAGGCATCTGAACTTGCAACGTTAATTCAGACAAAGAGGGAGCTTGGTTGTAGAGCTACA
  TACATCCAGACCATCGAAGAAGGAATTAACACACATACTCACGCAGCCAAAGACTTCTGGAAGCTCTTGG
  GTGGCCAGACCAGTTACCAGTCTGCCGGAGACCCAAAGGAAGATGAGCTGTACGAGACAGCCATCATAGA
  GACGAACTGTGTCTACCGCCTGACAGATGACAAACTCGTTCCTGATGATGACTACTGGGGGAAGATTCCC
  AAGTGTTCCCTTCTGCAGTCCAAAGAGGTCCTGGTGTTTGACTTTGGTAGTGAAGTTTACGTGTGGCATG
  GGAAGGAAGTCACGTTAGCACAACGGAAAATAGCATTCCAGCTGGCTAAGCACTTATGGAACGGAACCTT
  TGACTATGAGAACTGTGACATCAACCCCCTGGACCCCGGAGAGTGCAACCCGCTCATTCCCAGGAAAGGA
  CAAGGACGGCCTGACTGGGCAATATTTGGAAGAGTTACGGAGCACAATGAGACTATTCTGTTCAAAGAGA
  AATTCCTTGACTGGACAGAGCTGAAGAGACCCACAGAGAAGAATTCTGGAGAAGTTGTCCAGCAGAAGGA
  TGACCCGAGGGCCGACGTCAAGCCTTATGATGTGACACGGATGGTGGCGACGCCCCAGATTACAGCTGGC
  ACCATCTTAGATGGGGTGAACGTTGGCCGTGGCTATGGCCTGGTGGAAGGAGATGACAGAAGACAGTTTG
  AGATTGCCACTGTCTCTGTGGATGTGTGGCACATTCTGGAATTTGACTACAGCAGGCTCCCCAGGCAGAG
  CATCGGGCAGTTCCACGAGGGGGACGCCTATGTGGTCAAGTGGAAGTACATGGCAAGTACAGCAGTGGGA
  AGCCGACAAAAGGGAGAGCACCTGGTGAGAGTGGCTGGCAAAGAGAAGTGTGTCTATTTCTTCTGGCAAG
  GCCGGCACTCGACCGTGAGTGAGAAGGGAACGTCAGCTCTCATGACAGTAGAACTGGATGAAGAGAGGGG
  GGCCCAGGTCCAGGTCCTGCAGGGAAAGGAGCCACCCTGCTTCCTTCAGTGTTTCCAAGGGGGTATGGTG
  GTGCACTCCGGACGGAGGGAAGAGGAAGAAGAAAATGTGCAAAGTGAATGGAGACTGTATTGTGTGCGAG
  GAGAGGTGCCCATGGAAGGGAACTTGCTAGAAGTGGCCTGTCATTGCAGCAGTCTGAGGTCCAGGACTTC
  CATGGTCGTTCTGAATATAAACAAGGCCCTCATCTACTTGTGGCATGGATGCAAAGCTCAAGGTCATACG
  AAGGAGGTTGGAAGGACTGCAGCAAATAAGATCAAGGAAGAATGTCCCCTGGAAGCAGGTTTGCACAGCA
  GCAGCAACGTGACAATACATGAGTGTGACGAAGGATCCGAGCCCCTGGGATTCTGGGACGCTCTGGGGAG
  GAGGGACAGAAAGGCCTATGACTGCATGCTTCAAGATCCTGGAAGTTTTAACTTCGCGCCCCGCCTGTTC
  ATCCTCAGCAGCTCCTCCGGAGACTTCTCTGCGACAGAGTTTGTGTACCCCGCACAAGCGCCCTCTGCCG
  TCAGCTCCATGCCTTTCCTGCAGGAGGACCTGTACAGCGCGCCGCAGCCAGCTCTCTTCCTTGTTGACAA
  CCATCACGAGGTGTACCTCTGGCAAGGCTGGTGGCCCACTGAAAACAAGATAACCGGCTCTGCCCGCATT
  CGCTGGGCCTCAGACCGGAAGAGTGCCATGGAGACGGTCCTGCAGTACTGCCGAGGAAAGAATCTCAAAA
  GGCCACCCCCCAAGTCTTACCTTATTCATGCTGGGCTAGAGCCCCTGACATTCACCAACATGTTTCCCAG
  CTGGGAACACAGAGAAGACATTGCTGAAATCACAGAAATGGATACTGAAGTTTCCAACCAGATCACCTTG
  GTGGAAGATGTATTAGCCAAGCTCTGTAAAACCATCTATCCACTGGCAGATCTGCTTGCCAGGCCACTCC
  CAGAGGGGGTCGACCCTCTAAAGCTTGAGATTTATCTCACAGATGAAGACTTTGAGTTTGCACTCGACAT
  GTCCAGAGATGAATTCAATGCACTGCCCACCTGGAAGCAAGTGAACCTGAAGAAGTCAAAGGGCCTATTC
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153153
• Insert Size: 6513 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153153.3, NP_694793.1
• RefSeq Size: 7424 bp
• RefSeq ORF: 6513 bp
• Locus ID: 225115
• UniProt ID: Q8K4L3
• Cytogenetics: 18 A1
• Gene Summary: Isoform 1: Forms a high-affinity link between the actin cytoskeleton and the membrane. Is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a).