Myo10 (NM_019472) Mouse Untagged Clone

CAT#: MC225152

Myo10 (untagged) - Mouse myosin X (Myo10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myo10
• Synonyms: AW048724; D15Ertd600e; mKIAA0799; myosin-X
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225152 representing NM_019472
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCTTCTTTCCCGAGGGAGCACGGGTCTGGCTAAGAGAAAATGGCCAGCATTTTCCGAGTACTG
  TAAATTCCTGTGCAGAAGGCGTCGTGGTCTTCCAGACAGACTATGGGCAGGTGTTCACCTACAAGCAGAG
  TACAATCACCAACCAGAAGGTGACAGCTATGCACCCCCTGCACGAGGAGGGCGTGGACGACATGGCTTCC
  CTGGCCGAGCTCCATGGGGGCTCCATCATGTACAACCTTTTCCAGCGCTACAAAAGGAACCAAATCTATA
  CCTACATCGGCTCCATCATCGCGTCAGTGAACCCCTACCAGCCCATCGCTGGCCTGTACGAGCGCGCCAC
  CATGGAGGAGTACAGCAGGTGCCACCTGGGCGAGCTTCCACCGCACATCTTCGCCATTGCCAACGAATGC
  TATCGCTGCTTGTGGAAGCGCCATGACAACCAATGTGTCCTCATCAGCGGGGAAAGCGGAGCTGGGAAAA
  CGGAAAGCACCAAGCTGATCCTCAAGTTCCTGTCCGTCATCAGCCAGCAGACTTTGGACCTGGGCCTGCA
  GGAGAAGACATCCAGCGTCGAGCAAGCCATCCTTCAAAGCAGCCCAATCATGGAAGCATTTGGCAATGCG
  AAGACAGTATACAACAACAACTCCAGTCGCTTCGGGAAGTTTGTTCAGCTGAACATCTGCCAGCAGGGAA
  ACATTCAGGGAGGAAGAATTGTAGATTATTTATTAGAAAAAAACCGAGTAGTGAGGCAGAATCCCGGGGA
  GAGGAATTATCACATATTCTATGCACTGCTGGCAGGGCTGGACCAGGGAGAGCGAGAAGAATTTTATTTA
  TCTTTGCCAGAAAACTACCATTACTTGAATCAGTCTGGGTGTACGGAAGACAAGACGATCAGCGACCAGG
  AATCCTTCAGACAAGTTATCACGGCAATGGAAGTCATGCAATTCAGCAAGGAGGAAGTTCGGGAAGTTTT
  GAGGTTGCTGGCGGGAATTCTGCATCTCGGCAACATAGAGTTTATCACTGCTGGAGGGGCGCAGATTCCC
  TTCAAAACAGCTTTGGGTAGGTCTGCGGACTTGCTTGGGCTGGACCCAACGCAGCTCACAGATGCACTGA
  CCCAGAGATCCATGATCCTCAGGGGAGAAGAGATCCTGACGCCTCTCAGTGTCCAGCAGGCCGTAGATAG
  CAGAGACTCATTGGCCATGGCACTTTATGCTCGCTGCTTTGAGTGGGTGATCAAGAAGATCAACAGCCGG
  ATCAAAGGCAAAGACGACTTCAAATCCATCGGCATCCTTGACATCTTCGGATTTGAAAACTTCGAGGTTA
  ACCACTTTGAGCAGTTCAATATCAACTATGCAAATGAAAAGCTTCAGGAATACTTCAACAAACACATTTT
  TTCCTTAGAACAACTAGAATATAGCAGGGAGGGATTGGTGTGGGAAGATATTGACTGGATAGACAATGGA
  GAATGTCTAGACCTGATCGAGAAGAAACTTGGCCTCCTCGCCTTGATCAATGAAGAGAGCCATTTTCCTC
  AAGCCACAGACAGCACTTTGTTGGAGAAGCTACACAGTCAGCATGCTAATAACCACTTTTATGTGAAACC
  CCGCGTTGCAGTCAACAATTTTGGCGTGAAGCATTATGCAGGAGAGGTGCAGTATGATGTCCGAGGCATC
  TTGGAAAAGAACAGAGACACATTCAGAGATGACCTTCTCAACCTGCTCAGGGAAAGCAGGTTCGACTTCA
  TCTATGACCTCTTTGAACACGTCTCCAGCCGCAACAACCAGGACACCTTGAAATGCGGAAGCAAACACCG
  GCGGCCCACTGTCAGCTCGCAGTTCAAGGACTCCCTGCACTCCTTAATGGCAACACTAAGCTCCTCAAAT
  CCTTTCTTTGTCAGATGCATCAAGCCAAACACACAGAAGATGCCAGACCAGTTTGATCAGGTGGTAGTGC
  TCAACCAGCTGCGCTACTCGGGGATGCTGGAGACGGTGAGGATTCGGAAAGCAGGGTATGCCGTCAGAAG
  ACCCTTTCAGGACTTTTACAAAAGGTATAAAGTGCTGATGAGGAACTTGGCCCTGCCTGACGACATCCGA
  GGGAAGTGCACTGTGCTCCTGCAGGTCTATGACGCTTCTAACAGCGAGTGGCAGCTGGGGAAGACCAAGG
  TCTTCCTTCGAGAATCCTTGGAGCAGAAACTGGAAAAGAGGCGGGAAGAGGAGATTGACCGTGCGGCCAT
  GGTGATCCGAGCTCACATCTTAGGCTACTTGGCACGAAAGCAATATAGAAAGGTCCTCTGTGGTGTGGTG
  ACCATACAGAAGAATTACAGAGCTTTCCTTGCGAGGAAGAAATTTTTGCACCTGAAAAAGGCAGCCATCG
  TGTTCCAGAAGCAACTGCGAGGACAGCTGGCACGCAGGGTTTACAGGCAGCTGTTGGCTGAGAAACGGGA
  GCTGGAGGAAAAGAAGAGGCGGGAGGAAGAGAAGAAGAGAGAGGAAGAGGAAAGAGAAAGAGAGCGAGCG
  CAAAGAGAAGCTGACCTCCTCCGTGCCCATCAGGAGGCAGAGACAAGGAGGCAGCAAGAACTGGAAGCCT
  TGCAGAAGAGCCAGAGGGAAGCGGACTTGACCAGGGAGCTGGAAAAGCAAAGGGAGAACAAGCAGGTGGA
  GGAGATCCTCCGCCTGGAGAAGGAAATCGAGGATCTGCAGCGCATGAAGGAGCGGCAGGAGCTGTCGTTG
  ACCGAGGCCTCCCTGCAGAAGCTGCAGCAGCTGCGGGATGAGGAGTTGCGCAGGCTGGAGGATGAGGCCT
  GCCGCGCCGCCCAGGAGTTCTTGGAGTCGCTCAACTTCGACGAGATCGACGAGTGCGTCCGCAACATCGA
  GCGCTCCCTGTCGGTAGGGAGCGAGATTTCGGGCGAAGAGCTCAGTGAGCTTGCCGAGAGTGCCAGCGGA
  GAGAAGCCAAACTTCAACTTCAGCCAGCCCTACCCCGCGGAGGAGGAGGTGGACGAGGGCTTCGAGGCGG
  ATGACGACGCCTTCAAGGACTCGCCCAACCCCAGCGAGCACGGCCACTCCGACCAGCGAACGAGTGGCAT
  CCGGACGAGCGACGACTCATCCGAGGAGGACCCTTACATGAACTACACGGTGGTGCCCACCAGCCCCAGT
  GCCGACAGTACGGTGCTTCTTGCTGCGTCCATGCAGGACTCCGCTAGCCTGCACAACTCCTCGAGTGGGG
  AGTCCACCTACTGCATGCCGCAGAACAACGGCGACCTGCCCTCCCCCGACGGCGACTATGACTATGACCA
  GGACGATTACGAGGATGGCGCCATCACGTCAGGCAGCAGCGTGACTTTCTCCAACTCCTATGGAAGCCAG
  TGGTCCCCCGACTACAGGTATTCCGTGGGAACCTACAACAGTTCCGGAGCGTACCGGTTCAGCTCAGAAG
  GGGCGCAGTCCTCCTTTGAAGACAGCGAGGAGGACTTCGACTCCAGGTTTGATACAGATGATGAACTTTC
  CTACCGGCGAGACTCTGTGTATAGCTGCGTCACACTGCCCTATTTCCACAGTTTTCTGTACATGAAAGGT
  GGCTTAATGAACTCCTGGAAGCGCCGCTGGTGTGTCCTGAAGGACGAGACCTTCCTGTGGTTCCGCTCCA
  AGCAGGAAGCCCTCAAGCAAGGCTGGCTGCACAAGAAAGGCGGGGGCTCCTCCACGCTGTCCCGGAGGAA
  CTGGAAGAAGCGCTGGTTTGTCCTTCGCCAGTCGAAGCTGATGTACTTTGAAAACGATAGCGAGGAGAAG
  CTCAAGGGCACCGTGGAAGTCCGCACCGCCAAGGAGATCATAGACAACACAAGCAAAGAGAACGGGATCG
  ACATCATCCTGGCCGACAGGACCTTCCATCTCATCGCGGAGTCCCCCGAAGATGCCAGCCAATGGTTCAG
  CGTGCTGAGTCAGGTCCACTCGTCCACAGACCAGGAGATCAGAGAAATGCATGATGAGCAGGCAAACCCC
  CAGAATGCTGTGGGCACACTGGATGTCGGGCTGATTGATTCTGTATGCGCCTCTGACAGCCCTGATAGAC
  CCAACTCATTTGTGATCATCACAGCCAACCGGGTGCTGCACTGTAACGCGGACACCCCAGAGGAGATGCA
  CCACTGGATAACCCTGCTGCAGAGATCCAAGGGGGACACCAGGGTGGAGGGCCAGGAGTTCATCGTCAGA
  GGGTGGCTGCATAAGGAAGTGAAGAACAGTCCGAAGATGTCCTCCCTGAAACTGAAGAAACGGTGGTTCG
  TGCTGACGCACAATTCCCTGGACTACTACAAGAGCTCAGAGAAGAACGCTCTGAAACTGGGCACTCTGGT
  CCTCAACAGCCTGTGCTCCGTTGTGCCCCCAGACGAGAAGATATTCAAAGAGACAGGCTACTGGAATGTC
  ACCGTGTATGGGCGCAAACACTGTTACCGACTCTACACGAAACTACTGAACGAGGCGACTAGGTGGTCCA
  GCGCCATCCAGAATGTGACTGACACCAAGGCTCCAATCGACACGCCCACCCAGCAGCTGATCCAAGATAT
  TAAGGAGAACTGCCTCAACTCTGACGTGGTGGAGCAGATTTACAAGCGGAACCCGATCCTTCGCTACACG
  CATCACCCGCTGCACTCCCCGCTCCTGCCTCTTCCCTATGGGGACATAAATCTCAACCTGCTGAAAGACA
  AAGGCTACACGACCCTTCAGGACGAAGCCATCAAGATATTCAATTCTCTCCAACAACTGGAGTCCATGTC
  CGACCCCATCCCTATCATCCAGGGCATCCTGCAGACCGGGCACGACCTGCGGCCTCTGCGGGATGAACTG
  TATTGTCAGCTCATCAAACAGACCAACAAGGTGCCCCATCCCGGCAGCGTGGGCAACCTTTACAGCTGGC
  AGATCCTGACCTGCCTCAGCTGCACCTTCCTGCCGAGCCGCGGGATCCTCAAATACCTCAAGTTCCACCT
  GAAAAGGATACGGGAACAGTTCCCAGGAACCGAGATGGAGAAGTACGCCCTCTTTATCTACGAATCCCTT
  AAGAAAACCAAATGCAGAGAGTTTGTGCCTTCCCGAGATGAAATCGAAGCTCTGATCCACCGGCAGGAAA
  TGACGTCCACAGTGTATTGCCATGGCGGAGGCTCCTGCAAGATCACCATCAACTCCCACACCACAGCCGG
  GGAGGTGGTGGAGAAGCTGATCCGAGGGCTCGCCATGGAGGACAGCAGGAACATGTTTGCCTTGTTTGAG
  TACAACGGACAGGTGGACAAGGCCATCGAAAGCCGGACCATCGTAGCTGACGTCCTGGCAAAGTTTGAAA
  AGCTAGCTGCCACGTCAGAAGCCGGGGACGCGCCATGGAAATTCTACTTCAAACTTTATTGCTTCCTGGA
  CACCGACAGCATGCCAAAAGACAGCGTGGAGTTTGCGTTTATGTTTGAACAGGCCCACGAAGCCGTCATC
  CATGGCCACCACCCAGCCCCTGAGGAAAGCCTCCAAGTGCTGGCTGCCCTGAGACTGCAGTATCTGCAGG
  GCGACTATACCCCGCACACCTCCATCCCACCCCTCGAGGAGGTTTACTCTGTGCAGAGACTCAGAGCTCG
  CATTAGTCAGTCGACCAAAACCTTCACCCCGTACGAGCGCCTCGAGAAGAGACGGACCAGCTTCCTGGAG
  GGCACGCTGCGGCGGAGCTTCCGGACCGGGTCGGTGGTTCGGCAGAAGGCGGAGGAGGAGCAGATGCTGG
  ACATGTGGATCAAGGAGGAAGTCTGCTCGGCTCGCACCAGCATCATTGACAAGTGGAAGAAACTGCAGGG
  GATGAACCAGGAACAAGCCATGGCCAAGTATATGGCCCTGATCAAGGAGTGGCCTGGTTATGGATCAACA
  CTCTTTGATGTGGAGTGCAAAGAAGGCGGCTTCCCCCAGGAGCTCTGGCTGGGCGTCAGCGCAGACGCCG
  TGTCTGTCTACAAGCGTGGAGAGGGCAAACCGTTAGAAGTCTTCCAGTATGAGCACATCCTCTCGTTCGG
  GGCACCGCTGGCCAACACGTACAAGATCGTAGTTGACGAGCGGGAGCTGCTCTTTGAGACGAGTGAGGTG
  GTGGATGTGGCCAAGCTCATGAAAGCGTATATCAGCATGATTGTGAAGAAGCGCTATAGCACCACACGCT
  CTGTGAGCAGCCAGGGCAGCTCCAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019472
• Insert Size: 6189 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019472.2, NP_062345.2
• RefSeq Size: 8098 bp
• RefSeq ORF: 6189 bp
• Locus ID: 17909
• UniProt ID: F8VQB6
• Cytogenetics: 15 9.36 cM
• Gene Summary: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments (By similarity). Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. May play a role in neurite outgrowth and axon guidance. In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Plays a role in formation of the podosome belt in osteoclasts.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).