Pcm1 (NM_023662) Mouse Untagged Clone

CAT#: MC225131

Pcm1 (untagged) - Mouse pericentriolar material 1 (Pcm1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcm1
• Synonyms: 2600002H09Rik; 9430077F19Rik; C030044G17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225131 representing NM_023662
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACAGGAGGAGGTCCTTTTGAAGAAGTCATGCATGATCAGGACTTACCAAACTGGAGCAATGACA
  GTGTGGATGACCGACTCAACAATATGGAGTGGGGTGGCCAACAGAAGAAGGCCAATAGATCATCAGAAAA
  GAATAAGAAAAAGTTTGGTGTAGCAAGTGATAAGAGGGTAACTAACGCTATTTCTCCGGAGTCATCGCCA
  GGAGTTGGAAGGCGAAGAACAAAGATTCCACATACGTTTCCACACAGCAGATATATGACTCAGATGTCTG
  TTCCAGAGCAGGCAGAACTAGAGAAACTTAAACAGCGGATAAACTTCAGTGATTTAGATCAGAGAAGTAT
  TGGAAGTGATTCTCAAGGTAGAGCAACAGCGGCTAACAACAAACGTCAGCTTAGTGAAAACCGAAAGCCC
  TTCAACTTTTTGCCTATGCAGATTAATACTAACAAGAGCAAGGATGCTACTGCAAGTCTTCCAAAGAGAG
  AGATGACAACGTCAGCACAGTGCAAAGAGTTGTTTGCTTCTGCTCTAAGTAATGACCTTTTGCAAAACTG
  TCAAGTCTCTGAAGAAGATGGGAGAGGGGAGCCTGCAATGGAGAGCAGCCAGATTGTAAGCAGGCTTGTT
  CAAATCCGTGACTATATTACTAAAGCTAGTTCTATGCGAGAAGATCTTGTAGAGAAAAATGAAAGATCTG
  CCAATGTTGAGCGTCTTACTCATCTAATAGAGCATCTTAAAGAGCAAGAAAAGTCATATATGAAATTTCT
  CCAGAAAATCCTTGCCAGAGATCCTCAGCAGGAGCCTATGGAAGAGACAGAAAATTTGAAGAAACAACAT
  GATTTATTAAAAAGAATGTTACAACAGCAGGAGCAGCTGCGAGCTCTGCAGGGCCGACAGGCTGCTCTTC
  TAGCTTTGCAGCATAAAGCAGAGCAAGCTATAGCTGTGATGGATGACTCTGTTGTTACAGAAACTACAGG
  AAGCTTATCTGGAGTCAGCATCACATCTGAACTAAATGAAGAACTGAATGATTTAATTCAGCGTTTCCAT
  AATCAGCTTCGTGATTCTCAGCCTCCAGCTGTTCCAGACAACAGAAGACAGGCAGAAAGTCTTTCATTAA
  CTAGAGAGATTTCTCAGAGCAGAAATCCCTCAGTTTCTGAACATTTACCTGATGAGAAAGTACAGCTTTT
  TAGCAAAATGAGAGTACTACAGGAAAAGAAACAGAAAATGGACAAATTACTTGGAGAACTTCATAACCTT
  CGAGATCAGCATCTGAACAACTCATCATTTGTGCCTTCAACTTCTCTCCAAAGAAGTGGGGATAAGAGAA
  GTTCAACTGTAGCTCTCTCTGCTCCTGTGGGCTTTGCATCAGCTGTCAATGGAGAGGCCAATAGTCTTAT
  CTCATCTGTTCCCTGTCCTGCCACCTCTCTAGTTTCTCAGAATGAGAGTGAAAATGAAGGTCATCTAAAT
  CCAGCTGAAAAACTACAGAAGTTAAATGAAGTTCAAAAGAGATTGAATGAACTGAGAGAATTAGTTCATT
  ATTATGAACAAACATCAGATATGATGACAGATGCTGTAAATGAAAATACAAAAGATGAAGAAACTGAAGA
  GTCAGAATATGATTCTGAACATGAAAATTCTGAACCTGTTACTAACATTAGAAATCCACAGGTAGCTTCT
  ACCTGGAATGAAGTGAATACTAACAGTAATACACAGTGTGGTTCTAACAATAGAGATGGGAGACCAGTTA
  ATTCTAACTGTGAAATTAATAACAGATCTGCTGCCAACATCAGAGCTCTAAACATGCCTCCATTAGACTG
  TCGATACAATAGAGAAGGAGAGCAGAGGTTGCATGTAGCACATGGAGAAGATGAGGAAGAAGAAGTAGAA
  GAGGAAGGAGTCAGTGGGGCTTCATTATCTAGTCGTAGGAGCAGTCTGGTCGATGAAGCACCAGAAGATG
  AAGAATTTGAACAGAAGATTAGTAGACTCATGGCTGCAAAAGAGAAACTTAAACAGCTCCAAGATCTTGT
  TGCTATGGTGCAGGATGATGATGCAACTCAAGTGGTTGTCCCTGCTGCATCAAATTTGGATGATTTCTAT
  GCAGCAGAAGAAGATATCAAACAAAATTCCAATAATGCAAGAGAAAATTCTAATAAAATAGATACTGGAG
  TAAATGAAAAAACAAGAGAGAAATTTTATGAGGCTAAACTACAGCAGCAACAGAGAGAGCTTAAACAATT
  GCAGGAGGAAAGAAAGAAATTGATTGAAATTCAAGAGAAAATTCAAGCAGTGCAAAAGGCATGCCCTGAC
  CTTCAGCTCTCAGCCACTAGTATAAGTAGTGGTCCCACAAAAAAATATCTACCAGCTATTACTTCAACTC
  CAACTGTTAATGAAAATGACAGCAGTACAAGCAAGTGTGTTATTGATCCTGAAGATTCTTCAGTGGTGGA
  TAATGAGTTGTGGTCAGACATGAGAAGACATGAAATGTTAAGGGAAGAGCTACGGCAGAGAAGGAAGCAA
  CTTGAAGCTCTAATGGCTGAACACCAGAGGAGGCAAGGTCTAGCTGAGACCTCATCTCCAGTGGCTATAT
  CATTGAGAAGTGATGGATCTGAGAACCTGTGCACTCCTCAGCAAAGTAGAACAGAAAAAACAATGGCAAC
  TTGGGGAGGTTCTACTCAGTGTGCATTAGATGAAGAAGGAGATGAGGATGGCTATCTTTCAGAAGGAATT
  GTTCGCACAGATGAAGAAGAGGAGGAGGAGCAGGATGCCAGTTCCAATGATAACTTCCCTATATACCCTC
  CTAGCATGAATCAAAATTCTTACAATGTAAAGGAAACCAAAACCAGGTGGAAGAGCAATCGCCCTGTTTC
  AGCAGATGGAAATTACCGTCCTTTAGCCAAGACAAGGCAGCAGAATATCAGCATGCAACGACAAGAAAAC
  CTTCGATGGGTGTCAGAGCTCTCTTACATAGAAGAGAAGGAGCAGTGGCAAGAACAGATTAATCAGCTAA
  AGAAACAACTCGATTTCAGTGTCAATATTTGTCAGACTCTGATGCAAGACCAACAGACTTTATCTTGTTT
  GCTGCAGACTCTTCTAACAGGTCCGTACAGTGTTCTGCCTAGTAATGTGGCGTCCCCTCAAGTGCACCTT
  ATCATGCATCAGCTGAACCAATGCTACACACAGCTAACATGGCAGCAGAACAATGTCCAGAGGTTAAAAC
  AAATGCTAACTGAACTTATGCGCCAACAAAATCAGCACCCAGAAAAGCCTAGAAGCAAAGAAAGAGGCAG
  TAGTGCGTCACACCCTTCTTCTCCCAATTTATTTTGCCCTTTCAGCTTTCCAACACAGCCTGTAAATCTG
  TTTAACCTGCCTGGATTTACTAACTTTCCATCATTTGCACCAGGAATGAATTTCAGCCCTTTATTTCCTT
  CTAATTTTGGAGATTTTTCTCAGAATGTTTCTACACCTACTGAACAGCAGCAGCCATTAGCTCAAAATCC
  TTCAGGGAAAACAGAGTATATGGCTTTTCCAAAACCCTTTGAAAGCAGTTCTTCTCTTGGAGCAGAAAAG
  CAAAGGAATCAGAAACAGCCCGAAGAGGAAGCTGAAAACACTAAGACACCATGGTTATATGATCAAGAAG
  GTGGAGTAGAAAAACCATTTTTCAAGACTGGATTTACAGAGTCTGTAGAGAAAGCTACAAATAGTAACCG
  CAAAAATCAACCAGATACAAGCAGGAGAAGACGTCAGTTTGATGAAGAATCCTTGGAAAGCTTTAGCAGT
  ATGCCTGATCCTATAGACCCAACAACAGTAACTAAAACATTTAAAACAAGAAAAGCATCTGCCCAGGCCA
  GCCTGGCCTCTAAGGACAAAACTCCCAAATCAAAGAGTAAGAAGAGGAATTCTACTCAGCTGAAAAGTAG
  AGTTAAAAATATTGGGTATGAAAGTGCCAGTGTGTCTAGCACATGTGAACCTTGCAAAAATAGGAACAGA
  CATTCAGCCCAGACTGAAGAGCCTGTTCAAGCAAAATTATTCAGCAGAAAGAATCATGAACAGCTGGAAA
  AAATAATAAAATATAGTAGGTCTGCAGAAATATCTTCAGAGACTGGAAGTGATTTTTCCATGTTTGAAGC
  TTTACGGGATACTATTTATTCCGAAGTGGCTACATTAATTTCTCAAAATGAGTCTCGTCCACATTTTCTT
  ATTGAACTTTTCCATGAGCTGCAGCTACTTAATACAGACTACTTGAGACAGAGAGCTTTATATGCATTGC
  AGGATATCGTATCCAGACACATTTCTGAAAGTGATGAAAGAGAAGGAGAAAATGTAAAGCCTGTGAACTC
  TGGCACTTGGGTAGCATCCAATTCAGAGCTTACTCCGAGTGAGAGCCTTGTTACTACTGACGATGAAACA
  TTTGAGAAGAACTTTGAAAGAGAAACACATAAAGTAAGTGAACAGAATGATGCTGACAACGTGAGTGTCA
  TGTCCGTATCCTCCAACTTTGAGCCATTTGCAACAGACGACTTAGGTAACACTGTGATTCACTTGGATCA
  AGCATTAGCCAGAATGAGAGAGTATGAGCGCATGAAGACCGAGACAGAAAGTCACTCAAATATGAGATGC
  ACTTGCAGGGTTATTGAGGATGAAGATGGTGCTGCCGCCGCTGCCACAGTCAGTAATTCAGAAGAAACTC
  CCATTATTGAGAATCACAATTCACCACAACCTATAAGTGATGTTTCTGCCGTCCCCTGTCCTAGAATTGA
  TACTCAGCAACTGGACCGCCAAATTAAAGCAATTATGAAAGAAGTCATTCCTTTTTTGAAGGAGCACATG
  GATGAAGTGTGTTCGTCTCAGCTTCTCACTTCAGTAAGACGTATGGTCTTGACCCTTACCCAGCAGAATG
  ATGAGAGCAAAGAGTTTGTGAAGTTCTTTCATAAACAACTAGGAAGTATATTGCAGGATTCACTAGCAAA
  ATTTGCAGGCAGAAAGCTGAAAGACTGTGGAGAAGATCTTCTTGTGGAGATCTCTGAAGTGTTGTTTAAT
  GAATTGGCTTTCTTTAAGCTCATGCAAGACTTGGATAATAATAGCATAGCTGTTAAACAGAGGTGCAAGA
  GGAAAATTGAAGCAGCTGGAGTGAGGCAGTCTTATGCAAAAGAGGCCAAAAGGATTCTTGAAGGAGATCA
  TGGCTCACCTGCTGGAGAGATTGATGATGAAGACAAAGACAAGGATGAGACTGAAACTGTTAAGCAGACT
  CAGACTTCTGAGGTATATGATGCTAAAGGTCCCAAAAATGTAAGATCTGATGTTTCTGATCAAGAAGAAG
  ATGAAGAAAGTGAACGGTGTCCAGTGTCTATTAATCTGTCTAAAGCTGAAAGTCAGGCTTTAACTAATTA
  TGGAAGTGGAGAAGATGAGAATGAGGATGAAGAAATGGAAGACTTTGAAGAGAGTCCTGTGGACATCCAG
  ACTTCACTTCAGGCTAACACTGAAACTACAGAAGAAAATGAACATGACAGTCAGATTCTGCAACATGATC
  TTGAAAAAACACCAGAAAGTACAAATGTACCATCAGACCAAGAACCAACTAGTAAGAATGATCAAGACAG
  CTCTCCTGTGAAACCTTGTTATCTCAATATCTTGGAAAATGAGCAACAGTTAAATAGTGCTACCCATAAG
  GACTCGCTTACTACTACTGATTCATCTAAACAGCCTGAGCCTCTGCCATTACCCTTGGCTGCAAGTGAAA
  CCTTAGTGCCTAGAGTCAAAGAAGTGAAGTCTGCTCAGGAAACGCCTGAGAGCTCTCTGGCTGGAAGCCC
  TGATACTGAATCTCCAGTGTTAGTGAATGACTATGAAGCAGAGTCTGGTAACATAAGTCAAAAGTCGGAT
  GAAGAAGACTTTGTGAAAGTTGAGGATTTGCCGCTCAAACTGACAGTCTATTCAGAGGAAGAGCTAAGGA
  AGAAAATGATAGAAGAAGAACAGAAAAACCATTTATCTGGTGAAATATGTGAAATGCAGACAGAAGAATT
  AGCTGGAAATTCTCAGATACTGAAAGAACCTGAAACAGTGGGAGCCCAGAGTATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023662
• Insert Size: 6078 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023662.3, NP_076151.2
• RefSeq Size: 8398 bp
• RefSeq ORF: 6078 bp
• Locus ID: 18536
• UniProt ID: Q9R0L6
• Cytogenetics: 8 23.89 cM
• Gene Summary: Required for centrosome assembly and function (PubMed:12112146). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (By similarity). Required to anchor microtubules to the centrosome (By similarity). Involved in the biogenesis of cilia (By similarity).[UniProtKB/Swiss-Prot Function]