Smchd1 (NM_028887) Mouse Untagged Clone

CAT#: MC225114

Smchd1 (untagged) - Mouse SMC hinge domain containing 1 (Smchd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smchd1
• Synonyms: 4931400A14Rik; AW554188; mKIAA0650; MommeD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225114 representing NM_028887
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGAGGGCGCCAGCGATCCCGCCGGCCTCTCGGAGGGATCTGGGAGAGATGGCGCCGTCGACG
  GCTGTAGGACGGTGTACTTGTTTGACCGGCGCGGGAAGGACTCGGAGCTAGGGGATCGCGCACTGCAGGT
  CTCGGAGCACGCGGACTACGCGGGGTTCCGCGCTTCTGTGTGTCAGACAATTGGCATTTCATCTGAAGAA
  AAGTTTGTTATTACAACTACAAGTAGGAAAGAAATTACCTGTAATAATTTTGACCACACTGTTAAAGATG
  GAGTCACTCTGTACCTGCTGCAGTCAGTTGACCAGTCACTACTGACGGCGACGAAAGAAAGAATTGACTT
  TCTACCTCACTACGACACTCTAGTTAAAAGTGGCATGTATGAGTATTATGCGAGTGAAGGACAGAATCCT
  TTGCCATTTGCTCTTGCAGAGTTAATCGACAATTCATTGTCAGCTACTTCTCGAAATAATGGTGTTAGGA
  GAATACAGATCAAATTGCTTTTTGATGAAACACAAGGAAAACCTGCTGTCGCAGTGGTAGATAATGGAAG
  AGGAATGACCTCTAAGCAGCTTAACAACTGGGCTGTGTATAGGCTGTCAAAATTTACAAGACAAGGTGAC
  TTTGAAAGTGATCACTCAGGGTATGTCCGGCCATTACCAGTACCACGCAGTTTGAACAGTGACATTTCCT
  ATTTTGGTGTTGGAGGCAAACAGGCTGTTTTCTTTGTTGGGCAATCAGCCAGAATGATAAGCAAGCCTAT
  CGACTCCAAGGATGTTCATGAGCTGGTGCTTTCTAAAGAAGATTTTGAGAAGAAGGAAAAAAATAAAGAG
  GCGATATATAGTGGATATATTAGAAACAGAAAGCCAGCTGATTCTGCTCACATTACAAATGATGATGAAA
  GATTCCTACACAATCTTATTGAGGAAGAGAAAGAGAAAGACAGCTTTACAGCTGTGGTTATTACAGGGGT
  ACAGCCAGAACACATACAGTACTTGAAAAATTATTTACATCTTTGGACACGACAGCTAACGCATATTTAT
  CATTACTATATTCATGGACCAAAAGGAAATGAAATAAGTACAGCCAAAGCCATTGGACCTTTCAACAATA
  TTGATATTGAAATTTCTTTGTTTGAAAAGGGGAAGACACCTAAGATTATCAACCTAAGGGAAATACAAGA
  TGACATGCAGACATTGTATATAAACACAGCGTCTGATAGTTTTGAGTTCAAGGCTCACGTTGAAGGAGAC
  GGTGTAGTGGAAGGGGTTATCCGGTACCATCCCTTCTTATATGATAGAGAAACCTTTCCTGATGACCCAT
  GCTTTCCATCAAAGCTAAAGGATGAAGACGACGATGATGACTGTTTCATATCCGAGAAAGCAGCTCGAGG
  GAAGAGGCCGATCTTTGAGTGTTTTTGGAATGGACGGTTAATACCATATACATCAGTAGGAGACTTTGAC
  TGGTGTGCTCCTCCTAAGAAGAGAGGGCTTGTCCCAATTGAGTGCTTTAATAGAATATCTGGTGCATTAT
  TCACTAATGACAAATTTCAGGTCAGCACAAATAAACTGACATTTATGGATCTTGAGCTGAAATTGAAAGA
  TAAGAACACCCTCTTTACAAGAATCTTAAATGGACAGGAGCAGAGAATGAAAATTGACAGAGAATTTGCT
  TTATGGCTGAAGGATTGTCATGAAAAGCATGACAAACAAATAAAATTCACGCTTTTTAAAGGAATAATTA
  CCCGTCCAGATCTTCCCACTAAAAAGCAAGGCCCCTGGGCAACATTTTCAGCAATAGAATGGGATGGGAA
  AATATACAAAGCAGGCCAACTGGTCAAGACAATCAAGACACTTCCTCTCTGTTATGGAAGCATTGTTAGA
  TTTTTCCTTCATGGTGATCATGATGGAGAAGTGTATGCGACGGGAGGTGAAGTTCAGATTGCCATGGAAC
  CCCAGGCACTGTATGATGAAATAAAAACTGTGCCCATTGCAAAGCTGGATAGGACAGTAGCCGAGAAAAC
  AATTAGGAAATATGTAGAAGATGAAATGGCAAGGCTCCCTGATAGATTGTCTGTAACTTGGCCTGAAGGA
  GATGAATTATTGCCAAATGAAGTTAGGCCTGCTGGAACACCAATCGGTGCATTAAGAATTGAAATTCTAA
  ACAAAAAGGGGGAAGCAATGCAGAAACTCCCAGGGACAAGTCATGGAGGGTCAAAGAAGCTGCTGGTGGA
  ACTTAAAGTCATACTGCACACTTCAAGTGGAAATAAAGAAATCATTTCACATATCAGTCAGCATGGAGGG
  AAATGGCCTTACTGGTTTAAAAAAATGGAAAATATCCAGAAACTGGGTAATTATACATTGAAATTACAAG
  TTGTATTGAATGAAAGTAATGCAGATACCTATGCAGGAAGATCACTACCGTCCAAAGTAATTAAATTCTC
  TGTTAAAGAGGGCAAGCCAGAAAAGTTTTCGTTTGGTCTTCTGGACTCTCCTTTCCGTGTTGGAGTTCCA
  TTTAATATTCCTCTGGAGTTACAGGATGAATTTGGTCACACTACCCAACTATTGTCTGATATTGAGCCAG
  TTCTTGAAGCAAGTGGTCTGTCTTTACATTATGAAGGAATAACCAAAGGCCCGAATTGTGTAATTCAAGG
  TGTCGTAGCTAAAGGTCCAGTTAACTCTTGCCAGGGCAAGAATTTTAATCTGAAGGTTATTCTTCCTGGT
  TTAAAAGAAGACTCACAGATTTTGAAGATTAGATTGCTACCTGGTCCCCCTCATCAACTAAAGGTGAAAC
  CTGACTCAGAAGTTCTGGTTATAGAGAATGGAACAGCTTTCCCATTTCAGGTGGAAGTTGTGGATGAGTC
  AGACAACATAACAGCACAACCAAAGCTGATCGTCCACTGTAAGTTTCTAGGTGCTCCAAACCTACCAGTC
  TATACTGTGGATTGCAGTAGCTCTGGAACTAGCATCCTAACAGGGTCACCCATTCAAGTTCAGAACATTA
  AGAAAGACCAGAAGACACTGACCGCCAGGATTGAGATACCTAGCTGTAAAGATGTGTCACCTGTTGAAAA
  GACGATCAAGTTACTTCCCAGTAGCCACGCTGCTTGCCTGCAGATCTTTAGTGTAGAGGAGCAAAAGGCG
  ATCCAGATCAAACATCAGGATGAGGTTACTTGGGTAGCAGGTGATGTAATACGCAATCTAATTTTTCAAA
  TGTATGACGAAGGAGAAAGAGAAATCAATATTACACCATCCTTGGCAGAAAAAATTAAGGTTAATTGGAC
  TCCTGAGGTGAACAAAGAACATTTGGTACAAGGTCTCCTTCCTGATGTGCAAGTACCAACATCTGTGAAA
  GATGTCCGCTACTGCCATGTTTCTTTCCAAGATGACCACGTGTGTCTGGAGAGCGCCTTTACAGTAAGAC
  CACTTCCTGATGATCCCAAACATTTAAAATGTGAATTAAAAGGAGGAAAAACAGTGCAGATGGGCCAAGA
  GCTTCAAGGAGAAATAGTTGTAATAATTGCAGATCAGTATGGAAATCAGATTTCATCATTTTCACCTGAT
  TCCTTATCTACTTTGTCGATTACTGGAGATGGCCTTGACAGCTCAAACTTGAAAATCACCTTGGAGGCCA
  ACTCACAGAGCGTAAGTGTGCAAGGCATCAGGTTTACTCCAGGGCCTCCTGGACCCAAGGATCTGTGTTT
  TACTTGGCGAGAGTTTTCTGACTTTCTGCGCGTGCAACTGGTTTCTGGACCTCCAACCAAGCTGCTGCTT
  ATGGACTGGCCAGAGCTGAAAGAGTCCATTCCTGTGATTAATGGAAGACAATTAGAGAACCCTCTCATTG
  TTCAACTTTGTGATCAGTGGGATAATCCTGCTTTAGTCCCAAACGTTAAAATATGTCTCATAAAAGCAAG
  CAGCTTAAGGCTACTACCTTCAAACCAGCAGCATAAAACAGATGATAAGGGCAGAGCTAATTTGGGGGTG
  TTCACTGTTTGTGCTCCAAGGGGAGAACACACTGTGCAGGTTAAAGGTGTCTATAATAAGAGCACTATAG
  AAGGGCCTACAATTAAGTTAACAATTCTTCCAGACCCTGAAAAGCCCATTCGCCTCAATGTTAAATATGA
  CCAAGATGCCTCTTTTATAGCAGGGGACATATTCACTGATTTTATGGTTAGTGTGATTTCTGAAAGTGGT
  AGTGTCATCAAAAATATTAATCCAACACGTATTTCCATGAAAATGTGGAAGCTGTCCAGTGGCATGAGCC
  GGCCACCAGCAAATGCAGAAACCTTTAGTTGTAATAAAATAAAAGGTAACGACAAGGAAGATGGCTGCTT
  CTATTTCAGGGAGAAAACTATCCCTAACAAGGTGGGAGCATATTGTATCCAGTTTGATTTCATGATTGAT
  AAGACAAATATTCTAAGCAGCCAACAGGTTATAGTTGATGTTCTCCCCAACCAGCCTATGAAGTTGGTTC
  CTGACAGCCAACCAGCTACCCCAGCTGTTTCTAATGTCCGTTCAATTGCCAGCAGGACCTTGGTCAAAGA
  CCTGCGCCTCAGTATCACAGATAATTATGGTAATCACACTGGAATGGATCTGGTTGGCACTGTAGTAGCA
  ACCATTAAAGGCTTTAATGAGGAGGACACTGATACCCCACTCTTCATCGGGAAGGTTAGGACGCTTGAGT
  TTCCCTTTGTGAAAGGCTCTGCTGAAATCACGACTCTAGTGCTGGCAGAAAATAGTCCTGGAAGGGATAG
  TACTGAATACTTTATTATCTTTGAGCCTCGGCTGTCAACTGTATCAGGAACATTAGAATCATATAGCCTG
  CCATTTATGTTTTACAATGATGTTAAGAAGCAGCAACAAATGGCAGCGCTTACGAAGGAGAAGGACGAGT
  TATCCAAGTCCATCACTATGTACAGAAGTCTCTTCGATGCCAACAAGCAGCTTGTGGATGAAATGAAATG
  CCAAGCTGAAGAAGCAAAGTTAAAAGAGACCCAGCTACGAAATGAACTAAAAGCGTACAATATTGATATT
  CCTGCGACACAACAGACAACTCATATTGAAGCACTTTTGGAAAAAAAGATAACAGAACAGAACGAGCTTA
  AAAAGAGGCCCAGAAGGTTGTGTACTCTTCCAAACTACACTAAACGCAGTGGAGATATTTTGGGAAAGAT
  TGCACATCTAGCACAAATTGAAGATGATCGAGCTGCCATGGTTATCTCTTGGCATCTGGCAAGTGACATG
  GACTGTGTGGTTACCCTAACCACTGATGCCGCACGCGCTATCTATGACGAAACTCAAGGTCGCCAGCAAG
  TGTTGCCCCTTGATTCTATTTACAGGAAAACTCTTCCAGATTGGAAAAGACCTCTACCTCATTTCCGAAA
  TGGTAAATTACACTTTAAGCCCTTTGGAAATCCAGTCTTTGCCCGAGATTTGTTAACATTCCCAGATAAT
  ATTGAACATTGTGAAACAGTTTTTGGTATGCTGTTAGGAGACACCATTATTTTGGATAATCTGGATGCAG
  CCAATCATTATAGAAAAGAGGTTGTTAAAATCACACACTGCCCCACACTCCTGACTAGAGATGGAGATAG
  AATTAGAAGTAATGGGAAGTTTGGGGGCCTTCAGAATAAAGCTCCGCCAATGGACAAACTTCGGGGAATG
  GTCTTCGGAGCTCCAGTACCAAAGCAGTGTGTGGTCTTGGGGAAACAAATAGATCTTATTCAGCAGTATC
  GCACTGCTCTGTACAGGCTGAGCTCTGTGAACGAGGACTTGGACAATCAGCTGCAGTACCTTCACACTCC
  TGATATGAAGAAGAAAAAGCAAGAACTTGATGAACAGGAAAAAAGTCTGAAACGAATAGAGCAGAAACTC
  GGAATGACTCCTGTGCGCAGGTGTAATGAGTCACTTTGTCACTCACCAAAGATTGAGGTGACAGAGTGCC
  CAATCCCTACTAAAAGAATGAGAAGAGAATCCACCAGACAGAATAGAAGGCCAAAAGGTGACGTACCAAA
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028887
• Insert Size: 6024 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028887.3, NP_083163.3
• RefSeq Size: 7053 bp
• RefSeq ORF: 6024 bp
• Locus ID: 74355
• UniProt ID: Q6P5D8
• Cytogenetics: 17 E1.3
• Gene Summary: Non-canonical member of the structural maintenance of chromosomes (SMC) protein family that plays a key role in epigenetic silencing by regulating chromatin architecture (PubMed:26091879, PubMed:29887375). Promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments (PubMed:23754746, PubMed:23819640, PubMed:26391951, PubMed:28587678, PubMed:29887375). Plays a key role in chromosome X inactivation in females by promoting the spreading of heterochromatin (PubMed:18425126, PubMed:22841499, PubMed:26391951, PubMed:29887375). Recruited to inactivated chromosome X by Xist RNA and acts by mediating the merge of chromatin compartments: promotes random chromatin interactions that span the boundaries of existing structures, leading to create a compartment-less architecture typical of inactivated chromosome X (PubMed:29887375). Required to facilitate Xist RNA spreading (PubMed:29887375). Also required for silencing of a subset of clustered autosomal loci in somatic cells, such as the DUX4 locus (PubMed:23754746, PubMed:23819640, PubMed:28587678). Has ATPase activity; may participate in structural manipulation of chromatin in an ATP-dependent manner as part of its role in gene expression regulation (PubMed:26391951, PubMed:27059856). Also plays a role in DNA repair: localizes to sites of DNA double-strand breaks in response to DNA damage to promote the repair of DNA double-strand breaks (By similarity). Acts by promoting non-homologous end joining (NHEJ) and inhibiting homologous recombination (HR) repair (By similarity). Required during preimplantation development, probably acts by regulating chromatin architecture (PubMed:29900695).[UniProtKB/Swiss-Prot Function]