Otof (NM_031875) Mouse Untagged Clone

CAT#: MC225112

Otof (untagged) - Mouse otoferlin (Otof), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Otof
• Synonyms: MGC183613
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225112 representing NM_031875
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGATTGTTCACCTCAAGACTGTCTCAGAGCTCCGAGGCAAAGGTGACCGGATTGCCAAAGTCA
  CTTTCCGAGGGCAGTCTTTCTACTCCCGGGTCCTGGAGAACTGCGAGGGTGTGGCTGACTTTGATGAGAC
  GTTCCGGTGGCCAGTGGCCAGCAGCATCGACCGGAATGAAGTGTTGGAGATTCAGATTTTCAACTACAGC
  AAAGTCTTCAGCAACAAGCTGATAGGGACCTTCTGCATGGTGCTGCAGAAAGTGGTGGAGGAGAATCGGG
  TAGAGGTGACCGACACGCTGATGGATGACAGCAATGCTATCATCAAGACCAGCCTGAGCATGGAGGTCCG
  GTATCAGGCCACAGATGGCACTGTGGGCCCCTGGGATGATGGAGACTTCCTGGGAGATGAATCCCTCCAG
  GAGGAGAAGGACAGCCAGGAGACAGATGGGCTGCTACCTGGTTCCCGACCCAGCACCCGGATATCTGGCG
  AGAAGAGCTTTCGCAGAGCGGGAAGGAGTGTGTTCTCGGCCATGAAACTCGGCAAAACTCGGTCCCACAA
  AGAGGAGCCCCAAAGACAAGATGAGCCAGCAGTGCTGGAGATGGAGGACCTGGACCACCTAGCCATTCAG
  CTGGGGGATGGGCTGGATCCTGACTCCGTGTCTCTAGCCTCGGTCACCGCTCTCACCAGCAATGTCTCCA
  ACAAACGGTCTAAGCCAGATATTAAGATGGAGCCCAGTGCTGGAAGGCCCATGGATTACCAGGTCAGCAT
  CACAGTGATTGAGGCTCGGCAGCTGGTGGGCTTGAACATGGACCCTGTGGTGTGTGTGGAGGTGGGTGAT
  GACAAGAAATACACGTCAATGAAGGAGTCCACAAACTGCCCTTACTACAACGAGTACTTTGTCTTCGACT
  TCCATGTCTCTCCTGATGTCATGTTTGACAAGATCATCAAGATCTCGGTTATCCATTCTAAGAACCTGCT
  TCGGAGCGGCACCCTGGTGGGTTCCTTCAAAATGGATGTGGGGACTGTGTATTCCCAGCCTGAACACCAG
  TTCCATCACAAATGGGCCATCCTGTCAGACCCCGATGACATCTCTGCTGGGTTGAAGGGTTATGTAAAGT
  GTGATGTCGCTGTGGTGGGCAAGGGAGACAACATCAAGACACCCCACAAGGCCAACGAGACGGATGAGGA
  CGACATTGAAGGGAACTTGCTGCTCCCCGAGGGCGTGCCCCCCGAACGGCAGTGGGCACGGTTCTATGTG
  AAAATTTACCGAGCAGAGGGACTGCCCCGGATGAACACAAGCCTCATGGCCAACGTGAAGAAGGCGTTCA
  TCGGTGAGAACAAGGACCTCGTCGACCCCTATGTGCAAGTCTTCTTTGCTGGACAAAAGGGCAAAACATC
  AGTGCAGAAGAGCAGCTATGAGCCGCTATGGAATGAGCAGGTCGTCTTCACAGACTTGTTCCCCCCACTC
  TGCAAACGCATGAAGGTGCAGATCCGGGACTCTGACAAGGTCAATGATGTGGCCATCGGCACCCACTTCA
  TCGACCTGCGCAAGATTTCCAACGATGGAGACAAAGGCTTCCTGCCTACCCTCGGTCCAGCCTGGGTGAA
  CATGTACGGCTCCACGCGCAACTACACACTGCTGGACGAGCACCAGGACTTGAATGAAGGCCTGGGGGAG
  GGTGTGTCCTTCCGGGCCCGCCTCATGTTGGGACTAGCTGTGGAGATCCTGGACACCTCCAACCCAGAGC
  TCACCAGCTCCACGGAGGTGCAGGTGGAGCAGGCCACGCCTGTCTCGGAGAGCTGCACAGGGAGAATGGA
  AGAATTTTTTCTATTTGGAGCCTTCTTGGAAGCCTCAATGATTGACCGGAAAAATGGGGACAAGCCAATT
  ACCTTTGAGGTGACCATAGGAAACTACGGCAATGAAGTCGATGGTATGTCCCGGCCCCTGAGGCCTCGGC
  CCCGGAAAGAGCCTGGGGATGAAGAAGAGGTAGACCTGATTCAGAACTCCAGTGACGATGAAGGTGACGA
  AGCCGGGGACCTGGCCTCGGTGTCCTCCACCCCACCTATGCGGCCCCAGATCACGGACAGGAACTATTTC
  CACCTGCCCTACCTGGAGCGCAAGCCCTGCATCTATATCAAGAGCTGGTGGCCTGACCAGAGGCGGCGCC
  TCTACAATGCCAACATCATGGATCACATTGCTGACAAGCTGGAAGAAGGCCTGAATGATGTACAGGAGAT
  GATCAAAACGGAGAAGTCCTACCCGGAGCGCCGCCTGCGGGGTGTGCTAGAGGAACTCAGCTGTGGCTGC
  CACCGCTTCCTCTCCCTCTCGGACAAGGACCAGGGCCGCTCGTCCCGCACCAGGCTGGATCGAGAGCGTC
  TTAAGTCCTGTATGAGGGAGTTGGAGAGCATGGGACAGCAGGCCAAGAGCCTGAGGGCTCAGGTGAAGCG
  GCACACTGTTCGGGACAAGCTGAGGTCATGCCAGAACTTTCTGCAGAAGCTACGCTTCCTGGCGGATGAG
  CCCCAGCACAGCATTCCTGATGTGTTCATTTGGATGATGAGCAACAACAAACGTATCGCCTATGCCCGCG
  TGCCTTCCAAAGACCTGCTCTTCTCCATCGTGGAGGAGGAACTGGGCAAGGACTGCGCCAAAGTCAAGAC
  CCTCTTCCTGAAGCTGCCAGGGAAGAGGGGCTTCGGCTCGGCAGGCTGGACAGTACAGGCCAAGCTGGAG
  CTCTACCTGTGGCTGGGCCTCAGCAAGCAGCGAAAGGACTTCCTGTGTGGTCTGCCCTGTGGCTTCGAGG
  AGGTCAAGGCAGCCCAAGGCCTGGGCCTGCATTCCTTTCCGCCCATCAGCCTAGTCTACACCAAGAAGCA
  AGCCTTCCAGCTCCGAGCACACATGTATCAGGCCCGAAGCCTCTTTGCTGCTGACAGCAGTGGGCTCTCT
  GATCCCTTTGCCCGTGTCTTCTTCATCAACCAGAGCCAATGCACTGAGGTTCTAAACGAGACACTGTGTC
  CCACCTGGGACCAGATGCTGGTATTTGACAACCTGGAGCTGTACGGTGAAGCTCACGAGTTACGAGATGA
  TCCCCCCATCATTGTCATTGAAATCTACGACCAGGACAGCATGGGCAAAGCCGACTTCATGGGCCGGACC
  TTCGCCAAGCCCCTGGTGAAGATGGCAGATGAAGCATACTGCCCACCTCGCTTCCCGCCGCAGCTTGAGT
  ACTACCAGATCTACCGAGGCAGTGCCACTGCCGGAGACCTACTGGCTGCCTTCGAGCTGCTGCAGATTGG
  GCCATCAGGGAAGGCTGACCTGCCACCCATCAATGGCCCAGTGGACATGGACAGAGGGCCCATCATGCCT
  GTGCCCGTGGGAATCCGGCCAGTGCTCAGCAAGTACCGAGTGGAGGTGCTGTTCTGGGGCCTGAGGGACC
  TAAAGAGGGTGAACCTGGCCCAGGTGGACCGACCACGGGTGGACATCGAGTGTGCAGGAAAGGGGGTACA
  ATCCTCCCTGATTCACAATTATAAGAAGAACCCCAACTTCAACACGCTGGTCAAGTGGTTTGAAGTGGAC
  CTCCCGGAGAATGAGCTCCTGCACCCACCCTTGAACATCCGAGTGGTAGATTGCCGGGCCTTTGGACGAT
  ACACCCTGGTGGGTTCCCACGCAGTCAGCTCACTGAGGCGCTTCATCTACCGACCTCCAGACCGCTCAGC
  CCCCAACTGGAACACCACAGTCAGGCTGCTCCGGGGCTGCCACAGGCTGCGCAATGGGGGCCCCTCTTCT
  CGCCCCACAGGGGAGGTTGTAGTAAGCATGGAGCCTGAGGAGCCAGTTAAGAAGCTGGAGACCATGGTGA
  AACTGGATGCGACTTCTGATGCTGTGGTCAAGGTGGATGTGGCTGAAGATGAGAAGGAAAGGAAGAAGAA
  GAAAAAGAAAGGCCCGTCAGAGGAGCCAGAGGAGGAAGAGCCCGATGAGAGCATGCTGGATTGGTGGTCC
  AAGTACTTCGCCTCCATCGACACAATGAAGGAGCAACTTCGACAACATGAGACCTCTGGAACTGACTTGG
  AAGAGAAGGAAGAGATGGAAAGCGCTGAGGGCCTGAAGGGACCAATGAAGAGCAAGGAGAAGTCCAGAGC
  TGCAAAGGAGGAGAAAAAGAAGAAAAACCAGAGCCCTGGCCCTGGCCAGGGATCGGAGGCTCCTGAGAAG
  AAGAAAGCCAAGATCGATGAGCTTAAGGTGTACCCCAAGGAGCTGGAATCGGAGTTTGACAGCTTTGAGG
  ACTGGCTGCACACCTTCAACCTGTTGAGGGGCAAGACGGGAGATGATGAGGATGGCTCCACAGAGGAGGA
  GCGCATAGTAGGCCGATTCAAGGGCTCCCTCTGTGTGTACAAAGTGCCACTCCCAGAAGATGTATCTCGA
  GAAGCTGGCTATGATCCCACCTATGGAATGTTCCAGGGCATCCCAAGCAATGACCCCATCAATGTGCTGG
  TCCGAATCTATGTGGTCCGGGCCACAGACCTGCACCCGGCCGACATCAATGGCAAAGCTGACCCCTATAT
  TGCCATCAAGTTAGGCAAGACCGACATCCGAGACAAGGAGAACTACATCTCCAAGCAGCTCAACCCTGTG
  TTTGGGAAGTCCTTTGACATTGAGGCCTCCTTCCCCATGGAGTCCATGTTGACAGTGGCCGTGTACGACT
  GGGATCTGGTGGGCACTGATGACCTCATCGGAGAAACCAAGATTGACCTGGAAAACCGCTTCTACAGCAA
  GCATCGCGCCACCTGCGGCATCGCACAGACCTATTCCATACATGGCTACAATATCTGGAGGGACCCCATG
  AAGCCCAGCCAGATCCTGACACGCCTCTGTAAAGAGGGCAAAGTGGACGGCCCCCACTTTGGTCCCCATG
  GGAGAGTGAGGGTTGCCAACCGTGTCTTCACGGGGCCTTCAGAAATAGAGGATGAGAATGGTCAGAGGAA
  GCCCACAGATGAGCACGTGGCACTGTCTGCTCTGAGACACTGGGAGGACATCCCCCGGGTGGGCTGCCGC
  CTTGTGCCGGAACACGTGGAGACCAGGCCGCTGCTCAACCCTGACAAGCCAGGCATTGAGCAGGGCCGCC
  TGGAGCTGTGGGTGGACATGTTCCCCATGGACATGCCAGCCCCTGGGACACCTCTGGATATATCCCCCAG
  GAAACCCAAGAAGTACGAGCTGCGGGTCATCGTGTGGAACACAGACGAGGTGGTCCTGGAAGACGATGAT
  TTCTTCACGGGAGAGAAGTCCAGTGACATTTTTGTGAGGGGGTGGCTGAAGGGCCAGCAGGAGGACAAAC
  AGGACACAGATGTCCACTATCACTCCCTCACGGGGGAGGGCAACTTCAACTGGAGATACCTCTTCCCCTT
  CGACTACCTAGCGGCCGAAGAGAAGATCGTTATGTCCAAAAAGGAGTCTATGTTCTCCTGGGATGAGACG
  GAGTACAAGATCCCTGCGCGGCTCACCCTGCAGATCTGGGACGCTGACCACTTCTCGGCTGACGACTTCC
  TGGGGGCTATCGAGCTGGACCTGAACCGGTTCCCGAGGGGCGCTAAGACAGCCAAGCAGTGCACCATGGA
  GATGGCCACCGGGGAGGTGGACGTACCCCTGGTTTCCATCTTTAAACAGAAACGTGTCAAAGGCTGGTGG
  CCCCTCCTGGCCCGCAATGAGAATGATGAGTTTGAGCTCACAGGCAAAGTGGAGGCGGAGCTACACCTAC
  TCACGGCAGAGGAGGCAGAGAAGAACCCTGTGGGCCTGGCTCGCAATGAACCTGATCCCCTAGAAAAACC
  CAATCGGCCGGACACAAGCTTCATCTGGTTCTTGAACCCTCTCAAGTCTGCCCGCTACTTCCTGTGGCAT
  ACCTACCGCTGGCTACTCCTCAAATTCCTGCTGCTCTTCCTCCTGCTGCTGCTCTTCGCCCTGTTTCTCT
  ACTCTCTGCCTGGCTACCTGGCCAAGAAGATCCTTGGGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031875
• Insert Size: 5994 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031875.2, NP_114081.2
• RefSeq Size: 7125 bp
• RefSeq ORF: 5994 bp
• Locus ID: 83762
• UniProt ID: Q9ESF1
• Cytogenetics: 5 B1
• Gene Summary: Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).