Ptprb (NM_029928) Mouse Untagged Clone

CAT#: MC225110

Ptprb (untagged) - Mouse protein tyrosine phosphatase, receptor type, B (Ptprb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptprb
• Synonyms: 3230402H02Rik; C130094E24; Ptpz; Rptpb; VE-PTP; Veptp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225110 representing NM_029928
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGCATGGAGCCCTAACGGCCTTGTGGATAACACTGAGCGTCGTGCAGACTGGAGTGGCAGAGC
  AAGTGAAATGTAACTTCACACTGTTGGAGTCCAGGGTCTCTAGCTTGTCAGCGTCTATCCAGTGGAGGAC
  TTTCGCGTCACCCTGTAACTTTAGCCTCATCTACAGCAGTGATACCTCGGGGCCCATGTGGTGCCATCCT
  ATTCGGATAGACAACTTTACCTACGGATGTAACCCCAAGGATTTACAAGCAGGGACCGTCTATAACTTCA
  GGATTGTTTCTCTGGATGGAGAAGAGAGCACTCTGGTCTTACAGACAGATCCGTTGCCTCCTGCCAGGTT
  TGAAGTCAATCGGGAGAAAACAGCATCAACCACCCTGCAGGTCCGGTGGACTCCCTCTTCTGGAAAAGTC
  TCCTGGTATGAGGTGCAATTATTTGATCATAACAATCAAAAGATACAAGAAGTCCAAGTTCAAGAAAGTA
  CCACCTGGAGCCAATATACTTTTCTGAACCTCACTGAGGGTAACAGTTACAAAGTTGCCATCACAGCTGT
  TTCGGGAGAAAAGCGCTCCTTTCCGGTGTATATCAATGGCTCTACAGTACCATCTCCAGTGAAAGATCTT
  GGCATTTCCCCCAATCCTAATTCTCTCCTAATTTCCTGGTCTCGTGGTTCTGGGAATGTGGAACAATACA
  GGCTGGTGCTAATGGATAAAGGGGCCATCGTTCAAGACACAAACGTGGACAGGCGTGATACTTCTTATGC
  TTTTCACGAGCTGACCCCTGGCCACCTCTACAACCTCACTATTGTCACCATGGCCTCGGGACTGCAAAAC
  TCCAGGTGGAAACTGGTGAGGACCGCTCCCATGGAAGTCTCAAATCTGAAGGTGACAAATGACGGGAGGT
  TGACCTCTCTAAATGTGAAGTGGCAGAAACCCCCTGGGGATGTAGATTCCTACAGCATTACCCTGTCTCA
  CCAAGGGACCATCAAAGAATCCAAAACATTAGCACCTCCTGTTACTGAAACTCAATTTAAGGACTTAGTC
  CCTGGACGGCTTTACCAAGTGACCATCAGCTGCATCTCTGGTGAGCTCTCTGCTGAGAAGTCAGCAGCGG
  GGAGAACAGTTCCAGAAAAAGTGAGGAATCTGGTTTCCTACAACGAGATTTGGATGAAGTCCTTTACAGT
  GAACTGGACGCCCCCTGCTGGAGATTGGGAGCATTATCGTATCGTGCTCTTCAATGAATCCTTGGTCTTG
  CTCAACACCACAGTGGGAAAGGAAGAAACGCACTATGCCTTGGATGGCTTGGAGCTCATACCAGGAAGAC
  AGTATGAGATAGAAGTCATTGTTGAGAGCGGAAATCTGCGGAATTCCGAGCGCTGTCAAGGCAGGACAGT
  ACCCCTGGCTGTCCTCCAGCTTCGCGTCAAACACGCTAACGAAACTTCACTGGGCATCACGTGGCGGGCC
  CCTCTAGGCGAATGGGAGAAATACATCATTTCGTTGATGGACAGAGAGCTCTTGGTCATCCACAAGTCAC
  TCTCCAAAGATGCCAAAGAATTCACTTTTACAGACCTGATGCCTGGACGGAATTACAAGGCTACTGTCAC
  TAGCATGAGTGGAGATTTAAAACAGTCATCTTCAATCAAAGGAAGAACAGTGCCTGCCCAGGTGACTGAC
  CTGCACGTCAACAACCAAGGGATGACCAGTAGTCTGTTCACTAACTGGACAAAGGCACTGGGAGATGTAG
  AGTTCTACCAAGTTTTACTGATCCATGAAAATGTGGTTGTCAAGAACGAGAGTGTTTCCAGTGATACCAG
  CAGATACAGCTTCCGCGCCCTGAAACCCGGCAGCCTCTACTCCGTGGTGGTGACCACGGTGAGTGGAGGG
  ATCTCCTCCCGGCAGGTGGTGGCGGAAGGAAGAACAGTCCCGTCCAGCGTGAGTGGGGTGACAGTCAACA
  ATTCTGGCCGGAATGACTACCTCAGCGTTTCCTGGCTGCCGGCGCCTGGAGAAGTGGATCACTACGTGGT
  GAGCCTCTCCCACGAGGGCAAGGTGGATCAGTTCCTCATCATCGCCAAATCTGTCAGCGAGTGTTCCTTC
  AGCTCCCTCACCCCGGGCCGCCTCTACAACGTCACTGTAACCACCAAGAGCGGCAATTATGCAAGCCACT
  CCTTCACCGAGGAACGGACAGTGCCAGACAAGGTCCAGGGAATCAGTGTTAGCAACTCTGCCAGAAGCGA
  CTACTTAAAGGTGTCCTGGGTGCATGCCACTGGAGACTTTGACCACTATGAAGTCACCATCAAAAACAGA
  GAAAGCTTCATTCAAACCAAAACCATCCCCAAGTCAGAAAATGAGTGTGAATTTATTGAGCTGGTTCCTG
  GACGCCTGTACAGCGTCACTGTCAGTACAAAGAGTGGACAATATGAAGCCAGTGAACAGGGGACAGGGAG
  AACGATCCCAGAGCCTGTGAAGGATCTCACCCTTCTCAACAGGAGTACGGAGGATCTCCATGTGACTTGG
  TCAAGAGCCAATGGGGATGTTGATCAGTACGAGGTCCAGCTGCTCTTCAACGACATGAAAGTCTTCCCTC
  ATATTCACCTTGTGAACACAGCAACTGAGTATAAGTTCACGGCGCTCACGCCGGGGCGCCATTACAAAAT
  CCTCGTCCTGACCATCAGTGGCGATGTCCAGCAGTCAGCCTTCATTGAAGGCCTCACAGTTCCCAGCACT
  GTCAAAAACATTCACATTTCTGCCAATGGAGCCACGGATAGGCTGATGGTAACCTGGAGCCCTGGTGGCG
  GGGATGTGGACTCCTATGTGGTGTCTGCATTCAGACAGGACGAGAAGGTTGACTCTCAGACCATTCCCAA
  GCATGCCTCGGAGCACACGTTCCACAGGCTGGAGGCCGGAGCCAAGTACAGGATCGCCATTGTTTCTGTC
  AGTGGGTCCCTGAGAAACCAGATAGATGCGCTCGGACAGACAGTCCCAGCGTCTGTCCAGGGAGTCGTCG
  CAGCCAATGCATACAGCAGTAATTCCTTAACAGTAAGTTGGCAGAAAGCCCTTGGTGTGGCAGAAAGATA
  CGATATCCTGCTTCTAAACGAGAATGGGCTTCTTTTGAGCAACGTGTCAGAGCCAGCTACGGCAAGACAG
  CACAAATTTGAAGATCTAACGCCAGGCAAGAAATACAAGATGCAGATCCTGACTGTCAGCGGAGGCCTCT
  TCAGTAAAGAATCTCAGGCTGAAGGCCGAACAGTCCCAGCAGCTGTCACCAATCTGAGGATCACAGAGAA
  CTCCAGTAGATACCTGTCCTTCGGCTGGACCGCCTCGGAGGGTGAACTCAGCTGGTACAACATCTTCCTC
  TACAACCCAGACAGGACTCTTCAGGAGCGAGCTCAAGTTGACCCGCTAGTCCAGAGCTTCTCTTTCCAGA
  ACTTGCTACAAGGCAGAATGTACAAGATGGTGATTGTCACTCACAGTGGGGAGCTGTCCAATGAGTCATT
  TATATTCGGCAGAACAGTTCCTGCTGCCGTGAACCATCTCAAAGGCTCCCATCGGAACACGACAGACAGC
  CTGTGGTTCAGCTGGAGCCCAGCCTCCGGGGACTTTGACTTCTATGAGCTGATTCTTTACAATCCCAACG
  GCACGAAGAAGGAGAACTGGAAAGAAAAGGACGTGACAGAGTGGCGTTTCCAAGGTCTTGTTCCTGGAAG
  GAAATACACCCTGTATGTGGTGACTCACAGTGGGGACCTCAGCAATAAAGTCACAGGGGAGGGCAGAACA
  GCCCCAAGTCCTCCGAGTCTTTTGTCATTCGCTGATGTTGCAAACACCTCCTTGGCTATCACCTGGAAGG
  GACCCCCAGACTGGACAGATTACAATGACTTTGAGCTGCAGTGGTTCCCTGGAGATGCACTTACCATCTT
  CAACCCCTACAGCAGCAGAAAGTCAGAAGGACGCATTGTGTACGGGCTTCACCCAGGGAGGTCCTATCAA
  TTCAGTGTCAAGACTGTGAGCGGGGACTCCTGGAAAACCTACAGCAAACCAATTTCTGGGTCTGTGAGGA
  CAAAGCCAGACAAGATACAAAACCTGCATTGCCGCCCCCAGAACTCCACGGCCATTGCCTGCTCTTGGAT
  ACCTCCTGACTCCGACTTTGATGGCTACAGCATTGAGTGCCGAAAAATGGATACCCAAGAAATCGAGTTT
  TCCAGAAAGCTGGAGAAAGAAAAATCACTGCTCAACATCATGATGTTAGTACCTCATAAGAGGTACCTGG
  TGTCCATCAAGGTGCAGTCGGCCGGCATGACCAGTGAGGTGGTTGAAGATAGCACCATCACCATGATAGA
  CCGCCCGCCTCAACCGCCTCCACACATCCGTGTGAATGAAAAGGATGTGCTAATCAGCAAATCTTCCATC
  AACTTTACTGTCAACTGCAGCTGGTTCAGCGACACCAACGGAGCGGTTAAATACTTTGCTGTGGTGGTGA
  GAGAGGCCGACAGCATGGATGAGTTGAAGCCAGAACAGCAGCACCCTCTCCCTTCCTACCTGGAGTACAG
  ACACAACGCCTCCATCCGAGTCTACCAGACCAATTATTTTGCCAGCAAATGTGCTGAAAGTCCCGACAGC
  AGTTCTAAAAGTTTCAACATTAAGCTTGGAGCAGAGATGGACAGCCTCGGTGGCAAATGTGATCCCAGTC
  AGCAGAAATTCTGTGATGGACCGCTGAAGCCACACACCGCCTACAGAATCAGCATCCGGGCTTTTACACA
  GCTATTTGACGAGGACTTGAAAGAGTTCACCAAACCTCTCTACTCGGATACGTTCTTCTCTATGCCCATC
  ACCACAGAGTCAGAGCCCTTGTTTGGAGTTATTGAAGGTGTGAGTGCTGGCCTGTTTCTAATTGGCATGC
  TGGTGGCCCTTGTTGCCTTCTTCATCTGCAGACAGAAAGCTAGCCACAGCAGGGAAAGGCCATCTGCCCG
  GCTCAGCATTCGTAGGGACCGGCCTTTGTCTGTCCATCTGAATCTGGGCCAGAAAGGCAACCGGAAAACT
  TCTTGCCCCATAAAGATCAATCAGTTTGAAGGGCATTTCATGAAGCTGCAGGCAGACTCCAACTACCTTC
  TATCCAAGGAATATGAGGACTTAAAAGACGTGGGTAGAAGCCAGTCATGTGACATTGCCCTCTTGCCTGA
  GAATCGAGGGAAAAATCGATACAACAACATATTGCCTTATGATGCCTCAAGAGTGAAGCTCTGCAATGTC
  GATGACGACCCTTGCTCTGACTACATCAACGCCAGCTACATCCCCGGTAACAACTTCAGACGAGAATACA
  TCGCCACTCAGGGACCGCTTCCAGGCACCAAGGATGACTTCTGGAAGATGGCGTGGGAGCAGAACGTTCA
  CAACATCGTCATGGTGACCCAGTGTGTTGAAAAGGGCCGAGTGAAGTGTGACCATTACTGGCCAGCAGAC
  CAGGACCCCCTCTACTACGGTGATCTCATCCTACAGATGGTCTCGGAGTCCGTGCTCCCCGAGTGGACCA
  TCAGGGAGTTTAAGATATGCAGTGAAGAACAGTTGGATGCACACAGACTCATCCGTCACTTTCACTACAC
  GGTGTGGCCAGACCATGGGGTCCCAGAGACCACCCAGTCCCTGATCCAATTTGTGAGGACAGTCAGGGAC
  TACATCAACAGAAGCCCCGGGGCTGGGCCCACCGTAGTGCACTGCAGCGCTGGTGTGGGCAGAACAGGGA
  CGTTCGTTGCCCTGGACCGGATCCTCCAGCAGTTGGACTCTAAGGACTCCGTGGACATTTATGGGGCAGT
  GCATGACCTAAGACTCCACAGGGTTCACATGGTCCAGACCGAGTGTCAATATGTGTATCTGCATCAGTGT
  GTAAGAGACGTCCTCAGAGCAAAGAAACTGCGGAACGAGCAAGAGAACCCCCTGTTTCCGATTTATGAGA
  ATGTGAATCCAGAGTATCACAGAGATGCAATCTACTCGAGACATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029928
• Insert Size: 5997 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029928.2, NP_084204.2
• RefSeq Size: 11912 bp
• RefSeq ORF: 5997 bp
• Locus ID: 19263
• UniProt ID: B2RU80
• Cytogenetics: 10 D2
• Gene Summary: Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin.[UniProtKB/Swiss-Prot Function]