Otof (NM_001100395) Mouse Untagged Clone

CAT#: MC225108

Otof (untagged) - Mouse otoferlin (Otof), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Otof
• Synonyms: MGC183613
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225108 representing NM_001100395
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGATTGTTCACCTCAAGACTGTCTCAGAGCTCCGAGGCAAAGGTGACCGGATTGCCAAAGTCA
  CTTTCCGAGGGCAGTCTTTCTACTCCCGGGTCCTGGAGAACTGCGAGGGTGTGGCTGACTTTGATGAGAC
  GTTCCGGTGGCCAGTGGCCAGCAGCATCGACCGGAATGAAGTGTTGGAGATTCAGATTTTCAACTACAGC
  AAAGTCTTCAGCAACAAGCTGATAGGGACCTTCTGCATGGTGCTGCAGAAAGTGGTGGAGGAGAATCGGG
  TAGAGGTGACCGACACGCTGATGGATGACAGCAATGCTATCATCAAGACCAGCCTGAGCATGGAGGTCCG
  GTATCAGGCCACAGATGGCACTGTGGGCCCCTGGGATGATGGAGACTTCCTGGGAGATGAATCCCTCCAG
  GAGGAGAAGGACAGCCAGGAGACAGATGGGCTGCTACCTGGTTCCCGACCCAGCACCCGGATATCTGGCG
  AGAAGAGCTTTCGCAGCAAAGGCAGAGAGAAGACCAAGGGAGGCAGAGATGGCGAGCACAAAGCGGGAAG
  GAGTGTGTTCTCGGCCATGAAACTCGGCAAAACTCGGTCCCACAAAGAGGAGCCCCAAAGACAAGATGAG
  CCAGCAGTGCTGGAGATGGAGGACCTGGACCACCTAGCCATTCAGCTGGGGGATGGGCTGGATCCTGACT
  CCGTGTCTCTAGCCTCGGTCACCGCTCTCACCAGCAATGTCTCCAACAAACGGTCTAAGCCAGATATTAA
  GATGGAGCCCAGTGCTGGAAGGCCCATGGATTACCAGGTCAGCATCACAGTGATTGAGGCTCGGCAGCTG
  GTGGGCTTGAACATGGACCCTGTGGTGTGTGTGGAGGTGGGTGATGACAAGAAATACACGTCAATGAAGG
  AGTCCACAAACTGCCCTTACTACAACGAGTACTTTGTCTTCGACTTCCATGTCTCTCCTGATGTCATGTT
  TGACAAGATCATCAAGATCTCGGTTATCCATTCTAAGAACCTGCTTCGGAGCGGCACCCTGGTGGGTTCC
  TTCAAAATGGATGTGGGGACTGTGTATTCCCAGCCTGAACACCAGTTCCATCACAAATGGGCCATCCTGT
  CAGACCCCGATGACATCTCTGCTGGGTTGAAGGGTTATGTAAAGTGTGATGTCGCTGTGGTGGGCAAGGG
  AGACAACATCAAGACACCCCACAAGGCCAACGAGACGGATGAGGACGACATTGAAGGGAACTTGCTGCTC
  CCCGAGGGCGTGCCCCCCGAACGGCAGTGGGCACGGTTCTATGTGAAAATTTACCGAGCAGAGGGACTGC
  CCCGGATGAACACAAGCCTCATGGCCAACGTGAAGAAGGCGTTCATCGGTGAGAACAAGGACCTCGTCGA
  CCCCTATGTGCAAGTCTTCTTTGCTGGACAAAAGGGCAAAACATCAGTGCAGAAGAGCAGCTATGAGCCG
  CTATGGAATGAGCAGGTCGTCTTCACAGACTTGTTCCCCCCACTCTGCAAACGCATGAAGGTGCAGATCC
  GGGACTCTGACAAGGTCAATGATGTGGCCATCGGCACCCACTTCATCGACCTGCGCAAGATTTCCAACGA
  TGGAGACAAAGGCTTCCTGCCTACCCTCGGTCCAGCCTGGGTGAACATGTACGGCTCCACGCGCAACTAC
  ACACTGCTGGACGAGCACCAGGACTTGAATGAAGGCCTGGGGGAGGGTGTGTCCTTCCGGGCCCGCCTCA
  TGTTGGGACTAGCTGTGGAGATCCTGGACACCTCCAACCCAGAGCTCACCAGCTCCACGGAGGTGCAGGT
  GGAGCAGGCCACGCCTGTCTCGGAGAGCTGCACAGGGAGAATGGAAGAATTTTTTCTATTTGGAGCCTTC
  TTGGAAGCCTCAATGATTGACCGGAAAAATGGGGACAAGCCAATTACCTTTGAGGTGACCATAGGAAACT
  ACGGCAATGAAGTCGATGGTATGTCCCGGCCCCTGAGGCCTCGGCCCCGGAAAGAGCCTGGGGATGAAGA
  AGAGGTAGACCTGATTCAGAACTCCAGTGACGATGAAGGTGACGAAGCCGGGGACCTGGCCTCGGTGTCC
  TCCACCCCACCTATGCGGCCCCAGATCACGGACAGGAACTATTTCCACCTGCCCTACCTGGAGCGCAAGC
  CCTGCATCTATATCAAGAGCTGGTGGCCTGACCAGAGGCGGCGCCTCTACAATGCCAACATCATGGATCA
  CATTGCTGACAAGCTGGAAGAAGGCCTGAATGATGTACAGGAGATGATCAAAACGGAGAAGTCCTACCCG
  GAGCGCCGCCTGCGGGGTGTGCTAGAGGAACTCAGCTGTGGCTGCCACCGCTTCCTCTCCCTCTCGGACA
  AGGACCAGGGCCGCTCGTCCCGCACCAGGCTGGATCGAGAGCGTCTTAAGTCCTGTATGAGGGAGTTGGA
  GAGCATGGGACAGCAGGCCAAGAGCCTGAGGGCTCAGGTGAAGCGGCACACTGTTCGGGACAAGCTGAGG
  TCATGCCAGAACTTTCTGCAGAAGCTACGCTTCCTGGCGGATGAGCCCCAGCACAGCATTCCTGATGTGT
  TCATTTGGATGATGAGCAACAACAAACGTATCGCCTATGCCCGCGTGCCTTCCAAAGACCTGCTCTTCTC
  CATCGTGGAGGAGGAACTGGGCAAGGACTGCGCCAAAGTCAAGACCCTCTTCCTGAAGCTGCCAGGGAAG
  AGGGGCTTCGGCTCGGCAGGCTGGACAGTACAGGCCAAGCTGGAGCTCTACCTGTGGCTGGGCCTCAGCA
  AGCAGCGAAAGGACTTCCTGTGTGGTCTGCCCTGTGGCTTCGAGGAGGTCAAGGCAGCCCAAGGCCTGGG
  CCTGCATTCCTTTCCGCCCATCAGCCTAGTCTACACCAAGAAGCAAGCCTTCCAGCTCCGAGCACACATG
  TATCAGGCCCGAAGCCTCTTTGCTGCTGACAGCAGTGGGCTCTCTGATCCCTTTGCCCGTGTCTTCTTCA
  TCAACCAGAGCCAATGCACTGAGGTTCTAAACGAGACACTGTGTCCCACCTGGGACCAGATGCTGGTATT
  TGACAACCTGGAGCTGTACGGTGAAGCTCACGAGTTACGAGATGATCCCCCCATCATTGTCATTGAAATC
  TACGACCAGGACAGCATGGGCAAAGCCGACTTCATGGGCCGGACCTTCGCCAAGCCCCTGGTGAAGATGG
  CAGATGAAGCATACTGCCCACCTCGCTTCCCGCCGCAGCTTGAGTACTACCAGATCTACCGAGGCAGTGC
  CACTGCCGGAGACCTACTGGCTGCCTTCGAGCTGCTGCAGATTGGGCCATCAGGGAAGGCTGACCTGCCA
  CCCATCAATGGCCCAGTGGACATGGACAGAGGGCCCATCATGCCTGTGCCCGTGGGAATCCGGCCAGTGC
  TCAGCAAGTACCGAGTGGAGGTGCTGTTCTGGGGCCTGAGGGACCTAAAGAGGGTGAACCTGGCCCAGGT
  GGACCGACCACGGGTGGACATCGAGTGTGCAGGAAAGGGGGTACAATCCTCCCTGATTCACAATTATAAG
  AAGAACCCCAACTTCAACACGCTGGTCAAGTGGTTTGAAGTGGACCTCCCGGAGAATGAGCTCCTGCACC
  CACCCTTGAACATCCGAGTGGTAGATTGCCGGGCCTTTGGACGATACACCCTGGTGGGTTCCCACGCAGT
  CAGCTCACTGAGGCGCTTCATCTACCGACCTCCAGACCGCTCAGCCCCCAACTGGAACACCACAGGGGAG
  GTTGTAGTAAGCATGGAGCCTGAGGAGCCAGTTAAGAAGCTGGAGACCATGGTGAAACTGGATGCGACTT
  CTGATGCTGTGGTCAAGGTGGATGTGGCTGAAGATGAGAAGGAAAGGAAGAAGAAGAAAAAGAAAGGCCC
  GTCAGAGGAGCCAGAGGAGGAAGAGCCCGATGAGAGCATGCTGGATTGGTGGTCCAAGTACTTCGCCTCC
  ATCGACACAATGAAGGAGCAACTTCGACAACATGAGACCTCTGGAACTGACTTGGAAGAGAAGGAAGAGA
  TGGAAAGCGCTGAGGGCCTGAAGGGACCAATGAAGAGCAAGGAGAAGTCCAGAGCTGCAAAGGAGGAGAA
  AAAGAAGAAAAACCAGAGCCCTGGCCCTGGCCAGGGATCGGAGGCTCCTGAGAAGAAGAAAGCCAAGATC
  GATGAGCTTAAGGTGTACCCCAAGGAGCTGGAATCGGAGTTTGACAGCTTTGAGGACTGGCTGCACACCT
  TCAACCTGTTGAGGGGCAAGACGGGAGATGATGAGGATGGCTCCACAGAGGAGGAGCGCATAGTAGGCCG
  ATTCAAGGGCTCCCTCTGTGTGTACAAAGTGCCACTCCCAGAAGATGTATCTCGAGAAGCTGGCTATGAT
  CCCACCTATGGAATGTTCCAGGGCATCCCAAGCAATGACCCCATCAATGTGCTGGTCCGAATCTATGTGG
  TCCGGGCCACAGACCTGCACCCGGCCGACATCAATGGCAAAGCTGACCCCTATATTGCCATCAAGTTAGG
  CAAGACCGACATCCGAGACAAGGAGAACTACATCTCCAAGCAGCTCAACCCTGTGTTTGGGAAGTCCTTT
  GACATTGAGGCCTCCTTCCCCATGGAGTCCATGTTGACAGTGGCCGTGTACGACTGGGATCTGGTGGGCA
  CTGATGACCTCATCGGAGAAACCAAGATTGACCTGGAAAACCGCTTCTACAGCAAGCATCGCGCCACCTG
  CGGCATCGCACAGACCTATTCCATACATGGCTACAATATCTGGAGGGACCCCATGAAGCCCAGCCAGATC
  CTGACACGCCTCTGTAAAGAGGGCAAAGTGGACGGCCCCCACTTTGGTCCCCATGGGAGAGTGAGGGTTG
  CCAACCGTGTCTTCACGGGGCCTTCAGAAATAGAGGATGAGAATGGTCAGAGGAAGCCCACAGATGAGCA
  CGTGGCACTGTCTGCTCTGAGACACTGGGAGGACATCCCCCGGGTGGGCTGCCGCCTTGTGCCGGAACAC
  GTGGAGACCAGGCCGCTGCTCAACCCTGACAAGCCAGGCATTGAGCAGGGCCGCCTGGAGCTGTGGGTGG
  ACATGTTCCCCATGGACATGCCAGCCCCTGGGACACCTCTGGATATATCCCCCAGGAAACCCAAGAAGTA
  CGAGCTGCGGGTCATCGTGTGGAACACAGACGAGGTGGTCCTGGAAGACGATGATTTCTTCACGGGAGAG
  AAGTCCAGTGACATTTTTGTGAGGGGGTGGCTGAAGGGCCAGCAGGAGGACAAACAGGACACAGATGTCC
  ACTATCACTCCCTCACGGGGGAGGGCAACTTCAACTGGAGATACCTCTTCCCCTTCGACTACCTAGCGGC
  CGAAGAGAAGATCGTTATGTCCAAAAAGGAGTCTATGTTCTCCTGGGATGAGACGGAGTACAAGATCCCT
  GCGCGGCTCACCCTGCAGATCTGGGACGCTGACCACTTCTCGGCTGACGACTTCCTGGGGGCTATCGAGC
  TGGACCTGAACCGGTTCCCGAGGGGCGCTAAGACAGCCAAGCAGTGCACCATGGAGATGGCCACCGGGGA
  GGTGGACGTACCCCTGGTTTCCATCTTTAAACAGAAACGTGTCAAAGGCTGGTGGCCCCTCCTGGCCCGC
  AATGAGAATGATGAGTTTGAGCTCACAGGCAAAGTGGAGGCGGAGCTACACCTACTCACGGCAGAGGAGG
  CAGAGAAGAACCCTGTGGGCCTGGCTCGCAATGAACCTGATCCCCTAGAAAAACCCAACCGGCCTGACAC
  GGCATTCGTCTGGTTCCTGAACCCACTCAAATCTATCAAGTACCTCATCTGCACCCGGTACAAGTGGCTG
  ATCATCAAGATCGTGCTGGCGCTGCTGGGGCTGCTCATGCTGGCCCTCTTCCTTTACAGCCTCCCAGGCT
  ACATGGTCAAGAAGCTCCTAGGGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001100395
• Insert Size: 5979 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100395.1, NP_001093865.1
• RefSeq Size: 6907 bp
• RefSeq ORF: 5979 bp
• Locus ID: 83762
• UniProt ID: Q9ESF1
• Cytogenetics: 5 B1
• Gene Summary: Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) contains multiple coding region differences resulting in different 3' coding region and 3' UTR, compared to variant 2. It encodes isoform 1, which is shorter and has a distinct C-terminus, compared to isoform 2.