Tcf20 (NM_001114140) Mouse Untagged Clone

CAT#: MC225103

Tcf20 (untagged) - Mouse transcription factor 20 (Tcf20), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcf20
• Synonyms: 2810438H08Rik; mKIAA0292; SPBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225103 representing NM_001114140
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCGTTTCGGGAGCAAAGCAGTTACCACGGAAACCAGCAGAGCTACCCACAGGAGGTGCACAGCT
  CATCCCGCATAGAAGAGTTCAGCCCTCGCCAGGCCCAGATGTTCCAGAATTTTGGGGGAGCAGGTGGTGG
  TAGTAGTGGCACTGGCAGCAGCAGTAGCGGTCGACGAGGAACAGCTGCTGCAGCGGCAGCAATGGCTAGT
  GAGACCTCTGGCCATCAAGGCTATCAGGGTTTCAGGAAAGAAGCTGGAGATTTTTACTACATGGCAGGCA
  ACAAAGACACAGTGGCAGCAGGAACCCCACAGCCTCCTCAGCGAAGGCCTTCTGGGCCTGTACAGAGCTA
  TGGACCTCCCCAGGGGAGCAGCTTTGGCAATCAGTATGCGAGTGAGGGTCATGTGAGCCAATTTCAAGCA
  CAGCACTCTGCCCTTGGTGGTGTGTCTCATTATCAGCAGGATTACACAGGGCCTTTCTCTCCTGGGAGTG
  CTCAGTATCAACAGCAGGCCTCTAGCCAACAACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCA
  GCAACAGCAGCAGCAAGTACAGCAGTTGAGACAACAGCTTTACCAATCCCATCAGCCTCTGCCACAAACC
  ACTGGACAGCCAGCCTCTGGCTCATCGCATCTACAACCAATGCAGCGGCCCTCAACTCTGCCATCTTCTG
  CTGGTTATCAGTTAAGAGTAGGTCAGTTTGGACAGCACTACCAGTCTTCTGCTTCCTCCTCTTCCTCCTC
  CTCCTTCCCTTCTCCACAGCGTTTCAGTCAGTCTGGACAAAGCTATGATGGCAGTTACAGTGTGAATGCT
  GGATCTCAGTATGAAGGGCATAATGTGGGTTCTAATGCACAGGCTTATGGAACACAATCAAATTATAGCT
  ATCAACCTCAGTCTATGAAAAATTTTGAACAGGCAAAGATTCCACCAGGAAACCAGCAGGGGCAGCAGCA
  GCAACAGCAGCAACCACAACCTCAGCAACAGCAGCCGCAGCAACAGCAGCAACAGCAGCAGCAGCAGCAA
  CATCCTCCCCAGCATGTGATGCAGTACACAAATGCTGCCACCAAGATGCCTCTGCAAAGCCAGGTTGGGC
  AGTACAACCAGCCTGAGGTTCCTGTAAGGTCTCCCATGCAGTTCCACCAGAATTTCAGCCCTATTTCTAA
  CCCTTCTCCAGCTGCTTCTGTGGTTCAGTCTCCAAGCTGTAGCTCTACACCTTCTCCTCTCATGCAGAGT
  GGTGAGAATCTCCAGTGTGGGCAAGGCAATGTGCCCATGAGTTCCAGAAACCGAATTTTACAGCTAATGC
  CCCAGCTCAGTCCAACTCCATCAATGATGCCCAGTCCTAACTCTCATGCTGCAGGATTCAAAGGATTTGG
  ATTAGAAGGGGTGCCGGAAAAGCGGCTGACTGATCCTGGGTTGAGTAGTTTGAGTGCTTTGAGTAGTCAA
  GTGGCCAATCTTCCTAATACTGTCCAGCACATGTTACTTTCTGATGCTTTGACACCTCAGAAGAAGACGT
  CTAAGAGGCCCTCATCATCATCTAAGAAAGCAGATAGCTGTACAAACTCAGAAGGCTCCTCACAGCCTGA
  AGAACAACTAAAGTCCCCTATGGCTGAGTCATTGGATGGAGGTTGCTCTAGTAGTTCAGAAGATCAAGGT
  GAGAGGGTGAGGCAACTGAGTGGCCAGAGCACTAGCTCTGACACCACCTATAAGTGTGGCGCTTCAGAGA
  AAGCTGGCTCCTCACCAACACAAGGTGCTCAGAATGAGGCCCCTAGGCTCAGTACCAGTCCTGCAACTAG
  GGACGAAGCTGCCTCTCCAGGTGCTAAGGACACATCACTGTCATCTGAGGGGAACACAAAAGTCAATGAG
  AAGACAGTTGGGGTGATTGTCTCCCGAGAAGCCATGACAGGTCGGGTAGAAAAATCTGGTGGACAAGATA
  AAGGCTCCCAGGAGGATGATCCTGCTGCCAGTCAGAGACCACCTAGCAATAGTGGCGTAAAGGAAATCAG
  CCACACATCACTTCCACAGCCAGATCCTCCAGGAGGAGGGAGCAAAGGAAACAAGAATGGTGATAATAAT
  AGCTCTAACCACAATGGAGAGGGGAATGGCCCGAGTAGCCACTCCGCAGTAGGCCCAAGTTTCACAGGCA
  GGACTGAGCCTAGCAAGTCTCCTGGAAGTTTGCGCTATAGTTACAAAGAGAGTTTTGGGTCAGCTGTACC
  ACGAAATGTCAGTGGTTATCCTCAGTATCCTTCAGGACAAGAAAAGGGGGATTTTGGCAGCCATGGAGAG
  CGAAAGGGTAGAAATGAGAAGTTCCCAAGTCTTCTACAGGAAGTGCTTCAGGGCTACCACCACCATCCTG
  ACAGAAGGTATCCTAGAAGCGCACAGGAACATCAAGGGATGGCTAGTGGCCTGGAAGGAACTGCAAGGCC
  TAACATCTTAGTCAGTCAAACCAATGAATTAGCTAGCAGGGGCCTTCTGAACAAGAGTATTGGATCCCTG
  TTGGAAAATCCCCACTGGGGACCATGGGAAAGGAAGTCAAGCAGCACAGCTCCTGAAATGAAACAGATCA
  ATTTGTCTGACTATCCCATTCCCAGGAAGTTTGAGATAGAACCTCCATCATCAGCCCATGAGCCTGGAGG
  CTCCCTTTCTGAAAGGAGGTCAGTAATCTGTGATATATCCCCACTAAGACAGATTGTCAGGGACCCAGGG
  GCTCACTCATTGGGACACATGGGTACTGATGCCAGAATTGGGAGGAATGAACGTCTCAACCCAAGTTTAA
  GTCAGTCAGTCATTCTTCCTGGTGGGTTGGTGTCCATGGAAACAAAATTGAAATCCCAGAGTGGACAGAT
  AAAAGAGGAAGACTTTGAACAATCCAAATCTCAAGCTAGTTTCAACAAGAAATCTGGAGACCACTGCCAT
  CCTACCAGCATCAAACATGAGACTTACCGTGGCAATGCAAGTCCTGGAGCAGCAGCCCATGATTCCATTT
  CAGACTATGGCCCACAAGATAGCAGGTCCACACCAATGCGGCGGGTCCCTGGTAGAGTTGGTAGCCGGGA
  GACTATGAGGGGTCGGTCCTCTTCTCAGTACCATGACTTTGCAGAAAAATTGAAGATGTCTCCAGGCAGG
  AGCAGAGGCCCAGGGGGAGACCCTCATCACATGAACCCACATATGACCTTTTCAGAGAGGGCCAATAGGA
  GTTCTTTACATGCTCCTTTTTCTCCCAACTCAGAAAGCCTGGCCTCTGCTTACCACACAAACACCAGGGC
  TCATGCTTATGGGGACCCTAATACCGGTTTGAATTCCCAGCTCCATTATAAGAGACAGATGTACCAACAG
  CAACAAGAGGAGTATAAAGACTGGGCCAGCAGTTCTGCTCAGGGAGTGATCGCTGCTGCGCAGCATAGGC
  AGGAAGGCCCACGCAAGAGCCCAAGGCAGCAGCAGTTTCTTGACAGAGTACGGAGCCCCCTGAAAAATGA
  CAAAGACGGAATGATGTATGGCCCACCAGTAGGTACGTATCATGACCCAAGCACTCAGGAAGCTGGGCGC
  TGTCTCATGTCTAGTGATGGTCTGCCTGCCAAAAGCATGGAATTGAAGCACAGCTCTCAGAAGTTACAAG
  AGTCTTGTTGGGATCTTTCTCGGCAGACTTCTCCAGCCAAAAGCAGTGGTCCTCCAGGAATGTCTAATCA
  AAAACGGTATGGGCCACCCCATGAGCCAGATGGACATGGACTAGCCGAGTCTGCACAGTCATCCAAACCT
  AGTAACGTCATGTTACGGCTTCCTGGTCAAGAGGATCATTCTTCTCAAAATCCTTTAATCATGCGAAGGC
  GGGTTCGTTCTTTTATCTCCCCTATTCCCAGTAAGAGACAGTCACAAGATGTAAAAAACAGTAACGCTGA
  TGATAAAGGGCGCCTCCTCCACCCATCAAAAGAAGGTGCCGATAAAGCATACAATTCCTACAGCCATCTT
  TCTCACAGTCAGGATATCAAGTCTATTCCTAAGAGAGATTCCTCCAAGGACCTTCCAAACCCAGACAATA
  GAAACTGTCCTGCTGTTACCCTGACAAGTCCTGCTAAGACCAAAATACTGCCCCCACGGAAGGGACGGGG
  ATTAAAATTGGAAGCTATAGTTCAGAAGATCACATCTCCAAATATTAGGAGGAGTGCATCTGCAAACAGT
  GCTGAGGCTGGGGGAGACACAGTCACACTGGATGATATACTATCTCTAAAGAGTGGTCCTCCTGAAGGTG
  GCACTGTGGCTACTCAAGAGGCTGAGATGGAAAAGCGAAAGTGTGAGGTGGTATCTGACCTAGTCAGTGT
  AACTAACCAGGAATCGAATGTTGAGAAGCCTCTTCCAGGGCCTTCTGAAGAGTGGCGTGGCAGTGGGGAT
  GACAAAGTCAAGACAGAGGCACATGTAGAAACAGCTTCTACTGGAAAGGAACCCTCTGGTACTATGACAT
  CCACAGCTTCACAGAAGCCTGGTGGTAACCAAGGGAGACCAGATGGTTCCCTGGGTGGGGCAGCACCACT
  AATCTTTCCTGACTCAAAGAATGTAGCTCCAGTGGGCATATTGGCCCCTGAGGCAAATCCCAAGGCTGAA
  GAGAAAGAGAACGATACAGTCATGATTTCACCCAAACAAGAAAGTTTCCCCCCTAAAGGGTATTTCCCAT
  CAGGAAAGAAAAAGGGGAGACCAATCGGTAGTGTCAATAAGCAAAAGAAACAGCAGCAGCAACCACCTCC
  ACCTCCTCAGCCCCCTCAGATGCCAGAAGGTTCTGCAGATGGGGAGCCAAAGCCAAAAAAGCAGAGGCAA
  AGGAGGGAGAGAAGGAAGCCTGGGGCCCAGCCAAGGAAGCGGAAAACCAAACAAGCAGTTCCCATTGTAG
  AACCTCAAGAACCAGAGATCAAGCTAAAGTACGCTACCCAGCCACTAGATAAAACTGATGCCAAGAACAA
  GTCTTTTTTCCCTTACATCCATGTAGTAAATAAGTGTGAACTTGGAGCTGTTTGTACAATCATCAATGCT
  GAAGAAGAAGAACAGACCAAATTGGTGAGGAGCCGGAAGGGTCAGAGATCTCTGACCCCTCCCCCCAGCA
  GCACAGAAAGCAAGGTGCTCCCAGCTTCATCCTTTATGCTGCAGGGGCCTGTGGTAACAGAGTCTTCTGT
  TATGGGGCACCTGGTTTGCTGTCTCTGTGGCAAGTGGGCCAGTTACCGTAACATGGGTGACCTCTTTGGA
  CCCTTTTATCCCCAAGATTATGCAGCCACTCTTCCGAAGAATCCACCTCCTAAGAGGTCCTCAGAAATGC
  AGAGCAAAGTCAAGGTTCGGCACAAAAGCGCTTCTAATGGTTCTAAAACTGACACTGAGGAGGAGGAGGA
  GCAGCAGCAGCAGAAGGAGCAGAGGAGCCTGGCTGCTCATCCTAGGTTCAAGCGGCGCCACCGCTCAGAA
  GACTGTGGTGGAGGTCCTCGGTCCCTGTCCAGGGGGCTCCCTTGTAAAAAAGCAGCCACGGAGGGCAGCA
  GTGAAAAGACTGTTTCAGACACAAAGCCCTCTGTACCTACCACTTCAGAAGGTGGGCCTGAGCTGGAGTT
  ACAAATCCCTGAACTACCTCTTGACAGCAACGAATTTTGGGTCCATGAGGGTTGTATTCTCTGGGCCAAT
  GGAATCTACCTGGTCTGTGGCAGGCTCTATGGCCTGCAGGAAGCGCTGGAAATCGCCAGAGAGATGAAAT
  GCTCCCACTGCCAGGAGGCTGGCGCTACTTTGGGCTGCTACAACAAAGGCTGCTCCTTCCGATACCATTA
  CCCATGTGCCATTGACGCAGATTGTTTGCTGCATGAGGAGAATTTCTCGGTGAGGTGCCCCAAGCACAAG
  CCTCCCCTTCCATGCCCTCTCCCCCCCTTGCAGAACAAGACCGCAAAAGGCAGCCTCAGCACAGAGCAGT
  CCGAGCGGGGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001114140
• Insert Size: 5964 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114140.1, NP_001107612.1
• RefSeq Size: 7364 bp
• RefSeq ORF: 5964 bp
• Locus ID: 21411
• UniProt ID: Q9EPQ8
• Cytogenetics: 15 E1
• Gene Summary: Transcriptional activator that binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer variant and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.