Shroom3 (NM_015756) Mouse Untagged Clone

CAT#: MC225099

Shroom3 (untagged) - Mouse shroom family member 3 (Shroom3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Shroom3
• Synonyms: AL022960; D5Ertd287e; Shrm; Shrm3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225099 representing NM_015756
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTCCGGAGAACTTGGAGGAGCCTAGCGCCACGCCAAACCCCAGTAGGACCCCCACGGAGAGAT
  TCGTTTATCTGGAAGCGCTCCTAGAGGGAGGGGCTCCCTGGGGCTTCACCCTGAAAGGTGGCCTGGAGCG
  TGGAGAACCGTTAATCATTTCCAAGATTGAAGAAGGGGGCAAAGCCGACTCAGTGAGCTCCGGACTGCAA
  GCTGGGGACGAAGTCATACACATCAATGAGGTGGCGCTGAGCAGTTCCAGGAGAGAGGCTGTCTCCCTAG
  TGAAAGGGTCCTACAAGACCCTGCGGCTGGTGGTGCGCAGAGACGTGTGTGCGGCCCCGGGCCATGCTGA
  TCCTGGTACCTCTAAGTCCCTCAGCTCAGAGCTGCTCACCTGCAGTCCCCAGCACCGGAAAGCAACATGG
  TCAGGAGGGGTGAAGCTTCGGCTAAAGCAGAGGTGCAGTGAGCCTGCCACTCGCCCTCACTCGTGGCATA
  CCACCAAATTTGGGGAGACACAGCCTGATGTCAGCATGATGCAGATATCTCAGGGCACAATGGGCCCTCC
  TTGGCACCAGAGCTACCATTCCAGCTCTTCGACCAGTGACCTCTCCAACTACGACCATGCTTATCTGAGG
  CGGAGCCCTGACCAGTGCAGCTCCCAGGGGAGCATGGAAAGCCTGGAGCCCAGTGGCGGATACCCACCCT
  GCCATCTCCTTTCTCCTGCCAAGTCCACGAGCAGCATCGACCAGCTAGGCCACCTTCATAACAAAAGGGA
  CTCCGCATATAGCTCCTTCTCCACCAGTTCGAGCATCTTCGAGTACCCACCCCCTGGTGGCTCTGCCCGA
  GAACGTTCGGGTTCCATGGACGTGATTTCTGCCCGGGGTGGCCTCCTAGAAGGGATGAGGCAGGCAGACA
  TCCGCTACGTCAAGACGGTCTATGATACCCGGAGAGGAGTATCTTCAGAGTATGAGGTGAACCCTTCAGC
  CTTGCTCCTTCAAGGTAGGGATGCCCATGCCTCAGCCGACAGTCAGGGCTGTGCTAAATGGCACAGCATT
  CCTCGGGGCAAGGGAACGCCTTCCCCGTCTTGGAGCCAGCAGTGTTCTGGTTCCCTGGAGACTGCTACTG
  ACAACCTGCCCCAGAAGGCAGGCGCACCCCTGCCCCCCACCCGCAGTGATAGCTATGCAGCCTTCCGCCA
  TCGCGAGCGTCCCAGCTCCTGGTCCAGCCTTGATCAGAAAAGGTTCTGCCGGCCTCAGACAAACTCTTCA
  GGCTCCCAGAAAACCCCATTCGCTGAGGACCAGCTGCATACAGTCCCAGAGAGGAGTCCAGAGAACAGCC
  CCCCAGTGAAATCTAAGCATAATTATACCCAGAAGGCGCAGCCTGGCCAACCTCTGCTGCCCACTGGCAT
  CTATCCGGTACCTTCTCCGGAGCCTCACTTTGCCCAGGTGCCCCAGCCCTCTGTGAGTAGTAATGGCACC
  GTCTACCCTGCGCTAGTCAAAGAGAGTGGATACACAGCTGCTCAGGGAACCTGTAACAAGATGGCTACCT
  TAGATGAGAACGGGAATCAAAATGAAGCTAGCAGGCCTGGGTTTGCCTTCTGTCAGCCCCTAGAACACAA
  CTCGGTGACTCCGGTGGAGAAAAGGCCAGAACCCACGGCCAAATACATCTACAAAGTCCATTTTTCTTCA
  GTGCCTGAAAATGAAGACTCCTCCCTAAAGAGGCACATCACACCTCCCCACGGCCATAGTCCATATCCCA
  GCGAGAGAAAGAACATCCACGGTGGCAGCAGAGCGTGTTCCAATCACCACAGTCTCTCGTCCCCCCAGGC
  CCAGGCCCTGCACGTGGGTGATGACAGGAGGCCTTCCAGGCTCTCTCAGCCCTGGGAGGGTGACTTTCAG
  GAAGATCACAATGCCAACCTCAGGCAGAAAGTGGAGAGGGAGGGCCAAGGCCAGGGCCTGTCGGGCAACT
  CTGGCAGGACCAGATCGGCCTTCTCATCTCTGCAGAATATTCCAGAGAGTCTGAGAAGACAAAGCAACGT
  GGAGCTTGGAGAAGCCCAGGAGGTTCACCCCGGGGGCAGGTCCAAGGTGGAGGACCCTGGAAGGAAAGCA
  GGGGCTTCAGACATCAGGGGCTACCTGGACAGATCTGTTTCCTATCCGAGGCCCGAGGGGAAAATGAACG
  CCGTAGACTCCGTCCACAGTGCAGACTCCAGGTATGAGGAGTCCCCGGCCCCAGCGCTGCCACAGACATC
  CGGTGCGAGCCAGCGGCGGCTCAGCTCCAGCAGCTCCGCCGCCCCGCAGTACAGGAAGCCCCATTGCTCC
  GTGCTGGAGAAGGTTTCTAGAATAGAGGAACGAGAGCAGGGGCGCCACAGACCCCTGAGCGTGGGCAGCT
  CTGCATACGGCCCCGGCTACAGGCCTGGGAGGACCGGCCCGACCCCCAGTACTTCCAGCAGTGACCTCGA
  CGATCCAAAAGCCGGCAGCGTCCATTTCTCGGAGTCCACTGAACACCTGCGCAATGGGGAGCAGAACCCC
  CCAAACGGGGAGGCTAAGCAGGAAGAGGCTTCCCGGCCCCAGTGCAGCCATTTGATCCGGAGGGCCCCTG
  CGGACGGCCGTGGACCTCCTGCCCGAGGCGGCGAGCCCTCAAGGCCGGAGGCCCGCCTGCTCCGCAGCCA
  GAGCACCTTTCAGCTCTACAGTGAGGCGGAGAGGGAAGCCTCGTGGTCAGAGGACAGGCCCGGTACCCCT
  GAGTCGCCCCTGCTCGATGCGCCTTTCAGCCGCGCCTACAGAAACAGCATCAAGGATGCCCAGTCCCGCG
  TCCTGGGGGCCACGTCGTTTCGTCGCCGAGACCTGGAACCGGGCACCCCCGCCACCTCGAGGCCCTGGCG
  CCCGCGACCCGCCTCCGCCCACGTGGGGATGCGGAGCCCCGAGGCCGCGGTGCCCTCGTCCTCCCCGCAC
  ACCCCTCGGGAGCGACACAGCGTGACCCCGGCCGCGCCCCAGGCTGCCCGCCGAGGCCCACGCCGGCGCC
  TCACTGTCGAGCAGAAGAAGCGCTCCTACTCGGAGCCCGAGAAGATGAACGAGGTAGGGGTCTCGGAGGA
  GGCCGAGCCCACGCCCTGCGGCCCGCCGAGGCCCGCGCAGCCGCGCTTCTCGGAGAGCACCGTGGCCGAC
  CGGCGCCGCATCTTCGAGCGCGACGGCAAGGCCTGCTCCACGCTCAGCCTGTCGGGCCCCGAGCTCAAGC
  AGTTCCAGCAGAGCGCCCTGGCCGACTACATCCAGCGTAAGACGGGCAAGCGGCCCACAGGAGCCGCCTG
  CACACCGGAGGCCGGGCTGCGCGAGCGCGCACAGAGCGCCTACCTCCAGGCCGGCCCCGCGGCGCCCGAT
  GGCCCCGGCCTGGCCTCTGCCTGTAGCCTGAGCTCCCTGCGAGAGCCCGAGGCGCTGCCCCGCAAGGAGC
  ACACTCATCCGTCGGCCGCGGACGGCCCGCAGGCGCCCCGTGATCGCAGCAGCTCCTTCGCCAGCGGACG
  CCTAGTCGGGGAGCGACGTCGCTGGGACCCTCAGGTCCCCAGGCAGCTGCTCAGCGGAGCGAACTGCGAA
  CCGAGGGGCGTCCAGAGGATGGACGGGGCCCCGGGCGGGCCGCCTTCCTGGGGCATGGTGGCCGGGAAGG
  CCGGGAAGTCCAAGTCAGCCGAGGATCTGCTGGAGCGCTCCGACACACTGGCGGTCCCTGTGCATGTGAG
  GTCCAGGTCGTCTCCCACCTCCGACAAGAAAGGTCAGGATGTGCTCCTGAGGGAAGGCAGCAACTTTGGT
  TTTGTAAAGGATCCATGCTGTTTGGCTGGCCCTGGACCTAGGTCTCTCAGCTGCTCAGACAAAGGCCAGA
  ATGAGCTGGCATTGCCCCTGCACCACCCTACGCCCTGCTGGAACGGCTCAGGCTGCAAAGCCACTGTGGC
  TTCCTCAGCTCCTCCCGAATCTTCTGGAGCCGCGGATCATCTGAAGCAACGTAGAGCGCCTGGTCCTAGG
  CCACTCTCAGCAGGAATGCACGGCCACTTCCCAGACGCCAGAGCTGCCTCACTCAGCTCCCCTCTGCCAT
  CACCTGTCCCCTCCAGTTACCGGTCACAGCTTGCCATGGATCAGCAGACTGGGCAACAGCCTCCCTCATC
  CCCTGCCTCTGCAGTGACCCAGCCTACCAGTCCAAGAAGCCCTGAGCTCAGCAGCCCAGCATATGGGCTA
  GGGGAGGGGATGTGGAAGAGGACATCCCTGCCTCAGCGACCCCCTCCCCCTTGGGTGAAATGGGCCCACG
  CGGTCAGAGAGGACGGCCTTGCTGAGGACACCTTGGCGCCTGAGTTTGCGAACCTGAAGCACTATAGAAA
  CCAGCCGAGTCGTCCGAGTTCCTGCAGCACTTCTGACCCAGACACTCCAGGAAGGATCTCGCTCCGAATA
  TCGGAGTCAGCCCTTCAACCTTCCCCACCGCCCCGGGGGGACTATGATGACGAAGTGTTCATGAAGGACT
  TGCACCCCAAGGTTACTTCAAGCCCCACATTTGAAGCTCTTCCCCCACCCCCGCCACCTTCGCCACCGAG
  CGAGGAACCTCTAGTGAATGGCACAGATGACTTTCCTCCGCCTCCTCCTCCCCAAGCCCTGTGTGAGGTA
  CTGCTGGATGGTGAGGCATCCACGGAAGCTGGCAGCGGCCCCTGCAGAATTCCCAGGGTGATGGTCACAA
  GGGAAGGGCACGTGCCTGGGGCAGCCCATTCCGAAGGTAGTCAGATAATGACTGCCACACCACCCCAAAC
  CTCGGCCAAAGGTTCAGAAGCCGAGTCCAACACGCCTTCCAGCGCGAGCGCCCAGCCTCAGCTCAACGGT
  TCTCCTGGCAAGCAGCTCTGTCCCAGCCAGACCCGAAACCTCACTTATGAACCTGTCGAGAGAACTCAGG
  ATCTAGGAAAGAAAACTCACGCCGAGCCTCAGAAGACCTCAGAAGATATCCGGACAGAGGCTCTAGCCAA
  GGAAATTGTCCACCAAGACAAGTCTCTGGCAGACATTTTAGATCCTGACTCCAGGATGAAGACAACGATG
  GACCTGATGGAGGGTTTATTCCCTGGAGACGCCAGTGTGCTGATGGATAGTGGCGCAAAGAGGAAAGCCT
  TAGACATCACCGCCAGGCGTGCGGGATGTGAAGCCAAGGCGAGCGACCACAAGGAGGCAGTGAGTGTGCT
  GGTCAACTGTCCTGCCTACTACAGTGTCTCTGCTGCCAAGGCGGAGCTGCTCAACAAAATCAAAGACATG
  CCGGAGGAGCTGCAGGAGGAGGAGGGGCAGGAGGATGTCAATGAGAAAAAGGCTGAGCTTATCGGAAGCC
  TTACCCACAAGCTGGAGAGCCTCCAGGAAGCCAAGGGGAGCCTGCTCACGGACATCAAGCTGAACAATGC
  CCTGGGAGAGGAGGTGGAGGCTCTGATCAGTGAGCTCTGCAAACCCAACGAGTTTGACAAGTACAAGATG
  TTCATAGGGGACCTGGACAAGGTGGTCAACCTGCTGCTGTCCCTGTCTGGACGCCTGGCCCGCGTGGAGA
  ACGTCCTCAGAGGCCTTGGTGAAGACGCCAGCAAAGAGGAAAGGAGTTCTCTGAACGAGAAAAGGAAGGT
  TCTGGCTGGCCAGCATGAGGATGCACGGGAGCTCAAGGAGAACCTGGACCGGAGGGAGCGCGTGGTGCTG
  GATATCCTGGCCAACTACCTGTCCGCCGAGCAGCTGCAGGACTACCAGCACTTCGTGAAGATGAAGTCCA
  CACTCCTCATTGAGCAGCGGAAGCTGGATGACAAGATAAAGCTGGGCCAGGAGCAGGTCAGGTGCCTGCT
  GGAGTCGCTTCCCTCGGACTTCAGGCCCAAGGCAGGGGCCATCTCCCTCCCTCCAGCCCTCACCGGCCAT
  GGGACTCCTGGGGGGACATCTGTATTCGGTGGTGTTTTCCCAACGTTGACCTCTCCTCTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015756
• Insert Size: 5943 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015756.2, NP_056571.2
• RefSeq Size: 7210 bp
• RefSeq ORF: 5943 bp
• Locus ID: 27428
• UniProt ID: Q9QXN0
• Cytogenetics: 5 47.29 cM
• Gene Summary: Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).