Myh10 (NM_175260) Mouse Untagged Clone

CAT#: MC225095

Myh10 (untagged) - Mouse myosin, heavy polypeptide 10, non-muscle (Myh10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myh10
• Synonyms: 5730504C04Rik; 9330167F11Rik; Fltn; mKIAA3005; Myhn-2; Myhn2; NMHC-B; NMHC II-B; NMHCII-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225095 representing NM_175260
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAACC
  CTGCCACTCAAGCTGACTGGACAGCTAAAAAGCTGGTGTGGATTCCATCGGAACGCCATGGTTTTGAGGC
  AGCTAGTATTAAAGAAGAGCGGGGCGATGAGGTTATGGTGGAGCTGGCAGAGAATGGGAAGAAAGCAATG
  GTCAACAAAGATGACATTCAGAAGATGAACCCACCAAAGTTCTCCAAGGTGGAGGATATGGCAGAGCTGA
  CATGCTTGAACGAAGCCTCTGTCTTACATAATTTGAAGGACCGCTACTATTCAGGACTTATCTATACTTA
  CTCTGGACTCTTCTGTGTGGTGATAAATCCTTACAAGAACCTTCCAATTTACTCTGAGAATATTATTGAA
  ATGTATAGAGGGAAGAAACGCCATGAGATGCCACCACACATCTACGCCATATCAGAGTCTGCTTACAGAT
  GCATGCTTCAAGATCGTGAGGACCAGTCAATTCTATGCACGGGTGAATCGGGTGCCGGGAAGACAGAAAA
  TACCAAGAAAGTCATTCAGTACCTTGCCCACGTTGCTTCTTCTCACAAAGGAAGAAAGGACCATAATATT
  CCTGGGGAACTTGAACGGCAGCTTTTACAAGCAAATCCAATTCTGGAATCCTTTGGAAATGCGAAGACTG
  TGAAAAATGATAACTCATCTCGCTTTGGCAAGTTTATCCGGATCAACTTTGATGTAACTGGCTATATTGT
  TGGGGCCAACATTGAAACATACCTTCTGGAAAAGTCTCGTGCTGTTCGTCAAGCTAAAGATGAGCGTACA
  TTTCATATCTTTTATCAGTTGCTCTCTGGAGCAGGGGAACACCTGAAATCCGACTTACTCCTGGAAGGTT
  TCAACAACTACAGATTCCTCTCCAATGGCTATATTCCTATTCCTGGACAGCAAGACAAGGATAACTTCCA
  GGAGACCATGGAAGCCATGCACATCATGGGCTTCTCTCACGAAGAGATCCTCTCAATGCTTAAAGTCGTA
  TCTTCAGTGCTGCAGTTTGGAAACATCTCTTTCAAAAAGGAGAGAAACACTGACCAAGCCTCCATGCCGG
  AGAACACAGTCGCACAGAAGCTCTGCCACCTGCTCGGGATGAATGTGATGGAGTTCACTCGGGCTATCCT
  CACGCCCAGGATCAAGGTTGGCCGGGATTACGTACAGAAAGCCCAGACCAAAGAGCAGGCTGATTTTGCA
  GTGGAAGCATTGGCAAAAGCTACCTATGAGCGGTTGTTTCGCTGGCTCGTTCACCGCATCAATAAAGCGC
  TGGATAGGACCAAACGCCAGGGAGCTTCCTTCATTGGGATCCTGGATATTGCTGGTTTTGAAATTTTTGA
  GCTGAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACC
  ATGTTCATCCTGGAGCAGGAGGAGTACCAGCGAGAGGGCATCGAGTGGAACTTTATCGACTTCGGCCTGG
  ACCTGCAGCCCTGCATCGACCTGATAGAGAGACCTGCCAATCCCCCTGGCGTGCTGGCCCTCCTGGATGA
  AGAATGCTGGTTCCCCAAAGCTACAGATAAAACATTTGTTGAAAAGCTGGTTCAGGAGCAAGGTTCCCAC
  TCCAAGTTTCAGAAGCCGCGCCAACTGAAAGACAAAGCCGACTTCTGCATCATCCACTACGCGGGGAAGG
  TGGACTATAAGGCAGATGAGTGGCTGATGAAGAACATGGACCCGCTGAATGACAACGTGGCCACCCTCCT
  GCACCAGTCCTCGGACAGATTTGTGGCTGAGCTTTGGAAGGACGTGGACCGAATTGTAGGTCTGGATCAA
  GTCACTGGGATGACTGAGACCGCGTTTGGCTCTGCATACAAAACCAAGAAGGGCATGTTCCGAACCGTCG
  GGCAGCTCTACAAGGAGTCTCTCACCAAGCTGATGGCAACTCTCCGCAACACCAACCCCAACTTCGTCCG
  CTGCATCATTCCAAATCACGAGAAGCGGGCTGGGAAACTGGACCCGCACCTCGTGCTCGATCAGCTTCGC
  TGTAACGGCGTCCTGGAAGGGATCCGGATCTGTCGCCAGGGGTTCCCCAACCGGATAGTTTTCCAGGAAT
  TCAGACAGAGATATGAGATCCTAACTCCCAATGCTATTCCTAAAGGCTTCATGGATGGCAAACAGGCGTG
  TGAGCGAATGATCCGAGCTTTAGAACTGGACCCAAACCTGTATAGAATTGGACAGAGCAAGATATTTTTC
  CGAGCTGGAGTTTTGGCGCACTTAGAAGAAGAAAGAGATTTAAAAATCACTGATATCATCATCTTTTTCC
  AAGCTGTATGCAGAGGCTACCTCGCCCGAAAGGCCTTTGCCAAGAAACAGCAACAACTAAGTGCCTTAAA
  GGTCTTGCAGCGGAACTGTGCGGCGTACCTGAAGCTGCGACACTGGCAGTGGTGGCGTGTCTTCACGAAG
  GTGAAGCCTCTCCTCCAAGTGACCCGCCAGGAGGAAGAACTCCAGGCAAAAGATGAGGAGCTGCTGAAGG
  TGAAAGAGAAGCAGACAAAAGTGGAAGGGGAGCTTGAGGAGATGGAGCGGAAGCACCAGCAGCTGCTGGA
  AGAGAAGAATATCCTGGCAGAACAACTGCAAGCCGAGACCGAGCTCTTCGCTGAAGCAGAAGAGATGAGA
  GCAAGGCTTGCTGCCAAAAAGCAGGAACTGGAGGAGATTCTCCATGACCTCGAGTCCAGGGTGGAGGAGG
  AGGAAGAGCGGAACCAGATCCTACAGAATGAGAAGAAGAAGATGCAGGCGCACATTCAGGACCTAGAAGA
  ACAACTGGATGAGGAGGAGGGGGCCCGGCAAAAGCTGCAGCTGGAGAAGGTGACAGCAGAGGCTAAAATC
  AAGAAGATGGAAGAGGAGGTTCTGCTTCTCGAAGACCAGAATTCCAAATTTATCAAAGAAAAGAAACTCA
  TGGAAGACCGAATTGCTGAGTGTTCCTCTCAGCTGGCTGAAGAGGAAGAAAAGGCAAAAAACTTGGCCAA
  AATCAGGAATAAGCAAGAAGTGATGATCTCGGACTTAGAAGAACGCTTGAAGAAGGAGGAGAAAACTCGA
  CAGGAACTGGAAAAGGCCAAACGGAAGCTGGATGGGGAAACAACCGATCTGCAGGACCAGATCGCTGAGC
  TGCAGGCACAGGTCGATGAGCTCAAAGTCCAGTTGACCAAGAAGGAGGAGGAGCTTCAGGGGGCGCTGGC
  CAGAGGAGATGATGAGACACTGCACAAGAATAATGCACTTAAAGTTGCACGGGAGCTGCAGGCCCAAATC
  GCAGAGCTCCAGGAAGACTTTGAGTCTGAAAAGGCTTCAAGGAACAAGGCTGAGAAACAAAAACGGGACT
  TGAGTGAGGAGCTGGAAGCTCTGAAGACAGAGCTGGAGGACACCCTAGACACCACAGCAGCTCAGCAGGA
  ACTCCGCACAAAACGTGAGCAGGAAGTGGCAGAGCTGAAGAAGGCTCTTGAGGATGAAACTAAGAACCAC
  GAAGCTCAGATCCAGGACATGAGACAGAGGCATGCCACAGCGCTGGAGGAGCTTTCCGAGCAGCTGGAGC
  AAGCGAAAAGGTTCAAAGCCAACCTGGAGAAGAACAAACAGGGCCTGGAGACAGACAACAAGGAGCTGGC
  GTGTGAGGTGAAGGTGCTGCAGCAGGTGAAGGCGGAGTCAGAGCACAAGAGGAAGAAGCTGGATGCCCAG
  GTCCAGGAGCTCCATGCCAAGGTGTCAGAGGGTGACAGGCTCAGGGTAGAGCTGGCCGAGAAAGCAAACA
  AGCTACAGAATGAGCTGGATAATGTGTCAACCCTGCTGGAAGAAGCTGAGAAGAAAGGTATTAAGTTTGC
  GAAGGATGCAGCTGGTCTCGAGTCTCAACTACAGGACACACAGGAGCTCCTTCAGGAAGAGACACGGCAG
  AAACTGAACCTGAGCAGTCGGATCCGGCAGCTGGAGGAGGAGAAGAACAGCCTTCAGGAGCAGCAGGAGG
  AGGAGGAGGAGGCCAGGAAGAACCTGGAGAAGCAGGTGTTGGCTCTGCAGTCCCAGCTGGCTGACACCAA
  GAAGAAAGTGGACGATGACCTGGGGACAATCGAGAGTTTGGAGGAAGCCAAAAAGAAACTGCTCAAGGAT
  GTGGAGGCGCTGAGCCAGCGGCTGGAGGAGAAGGTCCTGGCGTATGACAAGCTGGAGAAGACCAAGAACC
  GGCTGCAACAAGAACTGGATGACCTGACGGTGGACCTGGACCACCAGCGCCAGATCGTCTCCAACTTGGA
  GAAGAAACAGAAGAAGTTCGACCAGCTGTTGGCAGAAGAAAAGGGCATCTCTGCTCGCTATGCAGAAGAG
  CGGGACCGGGCTGAAGCTGAGGCCAGAGAGAAAGAAACCAAAGCGCTCTCCCTGGCGCGGGCCCTTGAGG
  AGGCCTTGGAGGCGAAGGAGGAATTCGAGAGGCAGAACAAGCAGCTTCGAGCAGACATGGAAGACCTGAT
  GAGCTCTAAAGACGATGTGGGGAAGAACGTCCACGAGCTTGAGAAATCCAAGCGAGCCTTGGAGCAGCAG
  GTGGAGGAGATGCGGACCCAGCTGGAGGAGCTGGAGGACGAGCTGCAGGCCACTGAGGATGCCAAGCTCC
  GCCTGGAAGTCAACATGCAGGCCATGAAGGCCCAGTTTGAGAGGGACCTGCAAACCCGAGATGAGCAGAA
  TGAAGAAAAGAAGCGGCTGCTGCTTAAGCAGGTGCGGGAGCTCGAGGCAGAGCTGGAGGATGAGCGGAAA
  CAGCGGGCACTGGCTGTGGCGTCAAAGAAGAAGATGGAGATAGACCTGAAGGACCTGGAGGCTCAGATCG
  AGGCTGCGAACAAAGCCCGGGATGAAGTGATCAAGCAGCTTCGCAAACTTCAGGCACAGATGAAGGATTA
  CCAGCGTGAACTAGAAGAGGCTCGAGCATCTAGAGATGAGATTTTTGCTCAATCCAAAGAAAGTGAAAAG
  AAACTGAAGAGTCTAGAAGCAGAAATTCTTCAGCTGCAAGAGGAGCTGGCCTCATCCGAGCGAGCCCGCC
  GACACGCAGAGCAGGAGCGAGACGAGCTGGCTGATGAGATCGCCAACAGCGCCTCTGGAAAGTCTGCGCT
  GTTGGATGAGAAGCGGCGCCTGGAAGCGCGGATCGCACAGCTGGAAGAGGAGCTGGAGGAGGAGCAGAGC
  AACATGGAGCTGCTCAATGACCGCTTCCGCAAGACCACGCTGCAGGTGGACACACTGAACACAGAGCTGG
  CAGCAGAGCGCAGCGCTGCCCAGAAGAGTGACAATGCCCGCCAGCAGCTGGAGCGACAAAACAAGGAGCT
  GAAGGCCAAGCTGCAGGAGCTGGAGGGGGCAGTCAAGTCCAAGTTCAAGGCTACCATCTCAGCCCTGGAA
  GCCAAGATTGGGCAGCTGGAGGAGCAGCTTGAGCAGGAAGCCAAGGAGCGAGCAGCTGCCAACAAACTAG
  TCCGTCGAACAGAGAAGAAACTGAAAGAAATCTTCATGCAGGTTGAAGACGAGCGTCGGCATGCGGATCA
  GTATAAGGAGCAGATGGAGAAGGCTAATGCCAGGATGAAGCAGCTTAAACGACAGTTGGAAGAGGCTGAG
  GAAGAGGCCACACGTGCCAACGCATCTCGGCGTAAACTCCAAAGGGAGCTGGACGACGCCACTGAGGCCA
  ATGAAGGCCTGAGCCGCGAGGTCAGCACTCTCAAGAACCGGCTCAGGCGGGGCGGTCCAATCAGCTTTTC
  TTCAAGCCGATCTGGCCGGCGCCAGCTGCACATTGAGGGGGCATCGCTAGAGCTGTCAGATGACGACACA
  GAAAGTAAGACCAGTGATGTCAATGACACACAGCCACCCCAATCAGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175260
• Insert Size: 5931 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175260.2, NP_780469.1
• RefSeq Size: 7783 bp
• RefSeq ORF: 5931 bp
• Locus ID: 77579
• UniProt ID: Q61879
• Cytogenetics: 11 41.95 cM
• Gene Summary: Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9 (By similarity). Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.[UniProtKB/Swiss-Prot Function]