Chd5 (NM_001081376) Mouse Untagged Clone

CAT#: MC225077

Chd5 (untagged) - Mouse chromodomain helicase DNA binding protein 5 (Chd5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chd5
• Synonyms: 4930532L22Rik; AW060752; B230399N07Rik; CHD-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225077 representing NM_001081376
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGCCCGCTGGGCACCGAGGAGGAGCTGCCGCGGCTGTTCGCGGAGGAGATGGAGAACGAGGAGG
  AGATGTCAGAAGAAGAAGACGGTGGCCTTGAAGGCTTTGAGGACTTCTTCCCTGCAGAGCCCGTGAGTCT
  TCCCAAGAAGAAGCCGAAGAAGCTGAAAGAGAGCAAGTCCTCCAAAGGTAAAAGGAAGAAGAAAGAGGGG
  AGCAATGACGAGATGTCAGACAACGAGGAGGACCTGGAAGAGAAGTCAGAGAGCGAAGGTAGCGACTACT
  CCCCCACCAAGAAGAAGAAGAAGAAACTGAAGGAGAAGAAGGAGAAGAAGGAGAAGAAGGAGAAGCGGAA
  AAAGAGGGGTGAGGATGAAGATGATAACGACGACGGAGGCTTGAAGGAGCCCAAGTCCTCGGGACAACTC
  ATGGCGGAGTGGGGGCTGGACGACGTGGACTACCTGTTTTCTGAGGATGACTACCACACACTGACCAACT
  ACAAAGCCTTCAGCCAGTTCCTCAGGCCCCTTATTGCCAAGAAGAATCCGAAGATCCCCATGTCCAAAAT
  GATGACTGTCCTGGGGGCCAAGTGGCGAGAATTCAGCGCCAACAACCCGTTTAAGGGTAGCTCGGCAGCA
  GCAGCTGCGGCGGCCGTGGCCGCGGCCGTGGAGACAGTGACCATTGCTCCTCCCTTGGCCATCAGTCCCC
  AGCAAGTGCCCCAGACTCTGCCCATACGAAAGGCCAAGACCAAAGAGGGCAAGGGGCCCGGAGTGAGGAA
  GAAGAACAAAGGCGCCAAAGATTCCAAGAAAAAGGGCAGGGGCAAGAGAGTGGCAGGGCTCAAGTTCCGC
  TTCGGAGGGATCAGCAAGAGGAAGAAGGGTTCCTCGAGCGAGGAGGATGAACGGGAGGACTCAGACTTGG
  ACAATGCCAGCATCCACAGCTCTTCTGTGCGTTCTGAGTGCTCTGCAGCTCTGGGCAAGAAGAACAAGAG
  GAGGCGCAAGAAGAAGAGGATTGACGATGGTGACGGCTATGAGACAGACCACCAGGACTACTGTGAGGTG
  TGCCAGCAGGGAGGCGAGATCATTCTCTGTGACACCTGCCCGAGGGCATATCACCTAGTCTGCCTGGACC
  CGGAGCTGGAGAAGGCTCCAGAGGGCAAGTGGAGCTGCCCACACTGTGAGAAGGAGGGGATCCAGTGGGA
  GCCGAAGGATGATGATGAAGAGGAGGAGGAGGGCGGCTGCGAGGAAGAGGAGGATGACCACATGGAGTTC
  TGCCGCGTGTGCAAGGACGGTGGCGAGCTCCTGTGCTGTGATGCCTGTCCCTCCTCCTACCACTTGCACT
  GCCTCAACCCGCCGCTGCCGGAGATACCGAACGGTGAATGGCTCTGCCCGCGCTGTACATGTCCCCCGCT
  GAAGGGCAAAGTTCAGCGGATCCTACACTGGAGGTGGACAGAACCCCCGGCTCCCTTTGTGGTGGGGCTG
  CCAGGACCAGAGGTGGAGCCAGGCATGCCCCCGCCCAGGCCTCTGGAGGGCATTCCTGAGAGAGAGTTCT
  TTGTCAAGTGGGCTGGTCTCTCCTACTGGCACTGCTCCTGGGTGAAGGAGCTACAGTTGGAGCTGTACCA
  CACCGTGATGTATCGCAACTACCAAAGAAAGAATGACATGGACGAACCGCCACCCTTTGATTACGGCTCT
  GGCGATGAGGATGGTAAGAGCGAGAAACGGAAGAACAAGGACCCTCTCTACGCCAAGATGGAGGAACGCT
  TCTACCGCTACGGCATCAAGCCCGAGTGGATGATGGTTCACCGCATCCTGAACCACAGCTTTGACAAGAA
  GGGGGACATACATTACCTGATCAAGTGGAAGGACCTGCCCTACGACCAGTGCACCTGGGAGATTGATGAG
  ATTGACATTCCCTACTACGACAACCTGAAGCAGGCCTACTGGGGCCACAGGGAGCTGATGCTGGGGGAAG
  ATGCCAGGCTGCCCAAGCGGCTGGTGAAGAAGGGCAAGAAGCTGAAGGACGATAAACAAGAGAAGCCACC
  CGATACGCCCATCGTGGACCCCACAGTCAAGTTCGACAAGCAGCCGTGGTACATCGACGCCACAGGAGGC
  ACGCTGCACCCTTACCAGCTGGAGGGCCTTAACTGGTTACGCTTCTCCTGGGCCCAGGGCACCGACACTA
  TCCTGGCTGACGAGATGGGTTTGGGGAAGACAGTACAGACCATTGTGTTTCTCTATTCTCTGTACAAGGA
  GGGCCACTCCAAGGGGCCTTACCTGGTCAGCGCGCCCCTGTCCACCATCATCAACTGGGAACGGGAGTTT
  GAGATGTGGGCGCCTGACTTTTACGTGGTCACCTACACGGGGGACAAGGAGAGCCGCTCTGTGATCCGTG
  AGAACGAGTTTTCCTTTGAGGACAACGCCATTCGCGGTGGCAAGAAAGTATTCCGCATGAAGAAGGAAGT
  GCAGATCAAATTCCACGTGCTGCTCACCTCTTATGAGCTCATCACCATTGACCAAGCCATCCTGGGCTCC
  ATCGAGTGGGCCTGCCTCGTGGTGGACGAGGCCCACCGGCTCAAGAACAACCAGTCCAAGTTCTTTAGGG
  TCTTGAATAGCTACAAGATCGACTACAAGCTGCTGCTGACAGGGACCCCCCTCCAGAACAACCTGGAGGA
  ACTGTTCCATCTCCTCAACTTCCTGACTCCAGAGAGGTTCAACAATCTGGAAGGCTTCTTGGAGGAGTTT
  GCTGACATATCCAAGGAAGATCAGATCAAAAAGCTGCATGATCTATTGGGGCCGCACATGCTTCGGCGGC
  TCAAGGCCGACGTGTTCAAGAACATGCCGGCTAAGACAGAGCTGATTGTCCGAGTGGAGCTGAGCCAGAT
  GCAGAAGAAGTACTACAAGTTCATCCTGACTCGGAACTTTGAGGCGCTCAATTCCAAGGGGGGTGGCAAC
  CAGGTGTCTCTACTCAATATCATGATGGATCTGAAGAAGTGCTGCAACCATCCGTACCTCTTCCCTGTGG
  CTGCTGTGGAAGCCCCTGTACTGCCCAATGGCTCCTACGATGGCAGCTCCCTGGTCAAGTCTTCGGGGAA
  GCTCATGCTATTGCAGAAGATGCTCAAGAAACTGCGGGATGAAGGGCACAGAGTGCTGATCTTCTCCCAG
  ATGACCAAGATGTTGGACCTCTTGGAGGACTTCCTGGAGTACGAGGGCTACAAGTATGAGCGGATCGACG
  GTGGCATCACTGGGGGCCTCCGGCAAGAGGCCATCGACAGATTCAACGCTCCTGGGGCCCAACAGTTCTG
  CTTCCTGCTCTCAACACGTGCCGGTGGCCTGGGCATCAACCTGGCCACAGCAGACACGGTCATCATCTAT
  GACTCGGACTGGAACCCTCACAATGACATCCAGGCCTTCAGCCGTGCTCACCGCATCGGGCAGAACAAGA
  AGGTCATGATCTATCGCTTCGTGACACGGGCCTCCGTGGAGGAGCGCATCACTCAGGTGGCCAAGCGTAA
  GATGATGCTCACACACCTGGTGGTGCGGCCTGGCTTGGGCTCCAAGTCGGGCTCCATGACCAAGCAGGAA
  CTGGATGATATCCTTAAGTTCGGGACAGAGGAGCTTTTCAAGGACGACGTGGAAGGCATGATGTCCCAGG
  GCCAGAGGCCTACCACACCCATCCCTGACATACAATCTACCAAGGGCGGATCTCTGACCGCCGGCGCAAA
  GAAAAAGCACGGCAGCACCCCGCCAGGCGATAACAAAGATGTAGAAGACAGCAGTGTGATCCATTATGAC
  GACGCAGCCATATCCAAGCTGCTGGATCGGAACCAGGACGCCACGGATGACACCGAGCTGCAGAACATGA
  ACGAGTACCTGAGCTCCTTCAAGGTGGCACAGTATGTGGTCCGCGAGGAAGATGGCGTGGAGGAGGTAGA
  GAGGGAGGTGATCAAGCAGGAGGAGAACGTGGACCCTGACTACTGGGAGAAGCTGCTGCGCCATCACTAT
  GAACAGCAGCAGGAGGACTTGGCCCGCAACCTGGGCAAGGGCAAGCGCATCCGCAAGCAGGTCAACTACA
  ATGATGCCTCCCAGGAGGACCAGGAGTGGCAGGATGAGCTCTCGGACAACCAGTCGGAGTATTCCATCGG
  TTCTGAGGATGAGGACGAGGACTTTGAGGAGCGACCAGAAGGGCAGAGTGGACGACGACAATCCAGGAGG
  CAGCTTAAGAGTGACAGGGACAAGCCCCTGCCTCCCCTTCTGGCTCGGGTTGGGGGCAACATTGAGGTTC
  TGGGCTTCAATGCCAGACAGAGGAAGGCTTTTTTGAACGCCATCATGCGCTGGGGCATGCCGCCTCAGGA
  TGCCTTCAACTCCCACTGGCTGGTGCGGGACCTTCGAGGGAAGAGTGAGAAGGAATTTAGGGCCTATGTA
  TCACTCTTCATGCGCCACCTGTGTGAGCCGGGCGCAGACGGCGCGGAGACCTTCGCAGACGGCGTGCCCC
  GGGAGGGCCTGTCTAGGCAGCACGTGCTCACGCGTATTGGGGTCATGTCGCTGGTTAGGAAGAAGGTGCA
  GGAGTTTGAGCATGTCAACGGCAAGTACAGCACCCCAGACCTGGTCCCCGAGGGGGCCGAGGGCAAGAAG
  CCGGGCGAGGTCATCTCCTCAGACCCCAACACACCTGTGCCTGCCAGCCCTGCACAGCTTCCACCGGCCC
  CACTGGGCCTGACAGACAAAATGGAAGCCCAGCTGGGCTACACAGATGAGAAGGAGTCAGGCATGCAGAA
  GCCAAAGAAGTCCCTGGAAATCCAGACCCTGCCAACTGCCCTGGACAGAGTAGAGGGTGAAGACAAACAT
  CAGAGCTCAGACAGCAAGGACAGGGCTCGGGAGGAGAGGACAGAAGAGGTGGAGAAGGCCCAGGGGTCTC
  CAGAGCAGCCACTGAAAGAGGAAGTGCTGCCGGACAAGGAGCCGATCCCAGACAAGCCGGAGCTGAGCTT
  GGGTCACAGCGGGGATTTCAGGCCAGATGACCCCAAGACTGAGGAGAAGGAGCCCGGGGAGACTCAGCAA
  AACGGCGACAGAGAGGAAGATGAGGAAGGCAAGAAAGAAGACAAAAATGGGAAATTCAAATTCATGTTCA
  ACATTGCAGATGGCGGTTTCACAGAGCTGCACACTCTGTGGCAGAACGAGGAGCGGGCGGCTGTGTCCTC
  GGGGAAAATCTACGAAATCTGGCACCGCCGTCACGACTACTGGCTGCTGGCAGGCATTGTGACGCATGGC
  TATGCCCGCTGGCAGGACATTCAGAATGACCCGAGATACATGATCCTAAATGAACCTTTCAAGTCTGAGA
  TCCACAAGGGCAACTACTTGGAGATGAAGAACAAGTTTTTGGCCCGTAGGTTCAAGCTGCTGGAGCAGGC
  GCTGGTGATTGAGGAGCAGCTCCGGAGGGCAGCGTACCTCAACATGACCCAGGATCCCAACCACCCAGCC
  ATGGCGCTCAATGCTCGCCTGGCAGAGGTGGAGTGCCTTGCCGAGAGCCACCAGCACCTATCCAAGGAGT
  CCCTCGCCGGAAACAAGCCTGCCAATGCTGTCCTGCACAAGGTCCTAAACCAGCTGGAGGAGCTGCTGAG
  TGACATGAAAGCAGATGTGACACGCTTGCCCTCCATGTTGTCACGCATCCCCCCGGTGGCCGCCCGTCTG
  CAGATGTCTGAACGCAGCATCTTGAGTCGCCTGACCAACCGAGCTGGTGACCCCACCATCCAGCAGGGCG
  CTTTCGGCTCCTCTCAGATGTACAACAACAGCTTTGGGCCCAACTTCCGGGGCCCTGGACCGGGAGGGAT
  TGTCAACTACAACCAGATGCCCCTGGGGCCCTATGTGACTGACATCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001081376
• Insert Size: 5859 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081376.1, NP_001074845.1
• RefSeq Size: 9486 bp
• RefSeq ORF: 5859 bp
• Locus ID: 269610
• Cytogenetics: 4 E2
• Gene Summary: Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.