Anapc1 (NM_008569) Mouse Untagged Clone

CAT#: MC225074

Anapc1 (untagged) - Mouse anaphase promoting complex subunit 1 (Anapc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Anapc1
• Synonyms: 2610021O03Rik; AI047775; AI853536; Apc1; AW547281; Mcpr; tsg24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225074 representing NM_008569
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTG
  GCCGAGACCACTGCAAGCACCACCCTAACGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCCTCTGA
  GTTATGGTCTTCTGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACAATCCATGAGAAACAG
  AAGGAAAGCTGGCAGTTAAGAAAAGGTGTGAGTGAGATTGGAGATGCAGCTGACTACGATGAAGAGCTCT
  ATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAAAGCCAGGCACTGGCAGTGTACAAAGCGTT
  CACAGTGGACAGCACTGTCCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAG
  ATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGTTCATGTATGAACATGCATAGCA
  TAGACGGGAAGGATTATATAGCTTCCTTACCTTTTCAGGTGGCAAATGTTTGGGCCACTAAATATGGCTT
  GCTGTTTGAACGATGCAGTTCTTCACATGAGGTACCTCCAAGTCTACCTAGAGAACCTTTGCCTACTATG
  TTCAGCATGCTGCACCCACTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCAT
  CACGGGTACAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTCAT
  GACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTGCGGAGGGTAAAGCCAGAGGAAGAGAAT
  GCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAACTACTAGCAGCTCCCTGACAGCAC
  ATCTCAGAAGCCTCTCCAAAGGCGAATCCCCTGTGGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAG
  CCAGAGTCGCTCAACCTCTTCGCCCAGTCTACACTCTCGGTCGCCTTCCATTTCCAACATGGCGGCTCTA
  AGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGCGCAAAGATTCAACCTTTCAAGCC
  ACAGTCAGTCACCAAAGAGACACAGCATTTCTCACTCTCCGAGTGGCAGTTTCAACGACTCATTTTTGGC
  ACCAGAAACAGAGCCAATTGTTCCTGAGCTGTGCATTGATCACTTATGGACTGAGACACTTCCGAATATA
  AGAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCT
  TTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTAT
  CTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTC
  CTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGAC
  TTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTAC
  TCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTG
  AGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACA
  TTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAATGGCTCCATGGTTCGGAT
  CACTATCCCTGAAGTGGCCACCTCGGAATTAGTACAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCA
  AAAGAAGTAGCCATTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCAGGAGGACCCAGCTGTC
  ACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATG
  GACCCGAAGTTTTGACTTTGAAGGATCACTTTCCCCAGTCATTGCACCCAAAAAAGCAAGGCCTTCTGAT
  ACTGGATCTGACGAGGACTGGGAGTACTTACTGAACTCAGAGTACCATCGCAATGTTGAGTCTCATCTTT
  TGAACAAATCTCTATGTTTGACTGCTTTGGAAGTTTCAAATGCAAAGGATGAAGATTTTTCGCAGAACCT
  CAGTCTGGATTCTTCTACCCTTCTCTTTGCTCACATTCCTGCAATTTTCTTTGTTCTTCACCTGGTTTAT
  GAAGAGCTCAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCTCTTATCGACCTCCTTGTTCAGTTGG
  CAAGGGACTTAAAATTGGACTCTTACTTGGACCATTACTATCGAGATTCCCCAACTCTTGTCAAAACCAC
  TGGACAAGTATGCACCATTGACCAAGGTCAGATGGGATTTATGCATCATCCCCCATTTTTTACTTCTGAG
  CCACCAAGTATTTATCAGTGGGTGAGTTCATGCCTGAAGGGTGAAGGGATGCCACCCTATCCTTACCTTC
  CTGGGATTTGCGAGAGGAGCAGGCTAGTGGTCTTGAGCATTGCCCTCTATACCCTTGGTGATGAGAGCTG
  TGTGTCTGATGAAACTTGCCAGTACTTATCCAAAGTAACCTCAACTCCCCAAAAGCCACAAGCAGAGCAA
  GAAGAAAACAGGTTTACTTTCCGGCATTCTGCTTCCGTTTCTGTTCTGGCCGAGAGATTAGTTGTCTGGA
  TGGCCAGTGTAGGATTCACTTTAAGAGATCTGGAGACTCTTCCCTTTGGGATTGCTCTTCCCATCAGAGA
  TGCGATTTACCACTGTCGGGAGCAGCCTGATTCAGATTGGTCAGAAGCGGTCTGTCTCTTGATTGGACGT
  CAGGATCTTTCCAAGCAGGCTTGTGAAGGAAATTTACCCAGAGGCAAATCTGTGCTCTCATCAGAGGTGT
  CTTCAGGAACTGAGGCAGAGGAGGAAGATGATGGCATGAATGACTTGAACCATGAGGTTATGTCATTAAT
  ATGGAGTGAAGATTTACGGGTGCAGGATGTGCGAAGGCTGCTTCAAAGTGCACAGCCTGTCCGTGTCAAT
  GTGGTGCAGTACCCAGAACTCAGTGACCATGAGTTCATTGAAGAAAAGGAAAACAGACTGCTCCAGTTGT
  GTCAGCGAACTATGGCCCTTCCAGTAGGACGAGGAATGTTTACCTTGTTCTCATATCATCCTGTTCCAAC
  AGAGCCGTTGCCTGTTCCTAAATTGAATCTGACAGGGCGAGCCCCTCCACGAAACACAACTGTAGATCTT
  AATAGCGGAAACATCGACGTGCCTCCCAACATGGCCAGCTGGGCCAGCTTTCATAATGGTGTGGCTGCCG
  GCCTGAAGATAGCCCCAGCCTCCCAGATAGACTCAGCTTGGATCGTTTACAACAAGCCAAAGCATGCTGA
  GTTAGCCAATGAGTATGCCGGCTTTCTCATGGCCCTGGGTCTGAATGGGCACCTTACCAAGCTGGCTACT
  CTCAATATCCATGACTATTTGACCAAGGGCCATGAAATGACAAGCATTGGATTGCTACTTGGTGTTTCTG
  CTGCAAAGCTTGGCACCATGGACATGTCAATTACCCGGCTCCTCAGCATTCACGTTCCTGCTCTCTTACC
  CCCGACATCCACAGAGCTTGACGTGCCTCACAATGTCCAAGTGGCGGCTGTGGTTGGCATTGGCCTTGTG
  TATCAGGGCACAGCTCACAGACACACTGCGGAAGTCCTGTTGGCTGAAATAGGGCGGCCCCCTGGTCCAG
  AAATGGAATACTGCACTGACAGAGAGTCGTACTCCTTAGCTGCTGGCCTGGCCCTGGGCATGGTTTGCTT
  GGGGCATGGCAGCAATTTGATTGGCATGTCTGATCTCAATGTGCCTGAGCAGCTGTATCAGTACATGGTT
  GGAGGCCATAGGCGATTTCAAACTGGAATGCACAGGGAGAAACATAAGTCTCCAAGTTATCAGATCAAAG
  AAGGAGACACCATAAACGTGGATGTGACTTGTCCCGGTGCTACTCTGGCGTTGGCTATGATCTACTTAAA
  AACCAATAACAGGTCCATTGCTGATTGGCTGCGTGCTCCTGATACCATGTATTTGCTAGACTTTGTGAAA
  CCAGAATTCCTTTTGCTTAGGACACTTGCTCGGTGCTTGATTTTGTGGGATGATATCTTACCAAATTCCA
  AGTGGGTTGACAGCAATGTTCCTCAGATTATAAGAGAAAATAGTATCTCTCTGAGTGAAATTGAATTGCC
  TTGTTCAGAGGACTTGAATTTGGAAACCTTGTCGCAAGCACACGTCTACATCATTGCAGGAGCCTGCTTG
  TCTCTAGGGTTTCGATTTGCTGGCTCAGAAAACTTATCAGCATTTAGCTGTCTGCATAAATTTGCAAAAG
  ATTTTATGAATTATTTATCTGCACCCAATGCTTCTGTAACAGGGCCCTATAACCTCGAAACCTGCCTGAG
  TGTGGTCCTGCTGTCTCTTGCCATGGTGATGGCTGGCTCTGGGAACCTGAAGGTGTTGCAGCTCTGTCGC
  TTTCTGCACATGAAGACTGGTGGAGAGATGAACTATGGCTTCCACTTGGCCCACCACATGGCCCTGGGCC
  TTCTCTTTTTGGGAGGAGGAAGGTACTCTTTGAGCACATCCAACTCATCCATTGCTGCCCTTCTCTGCGC
  CCTTTACCCACATTTCCCAGCCCACAGCACTGACAACCGGTATCATCTCCAGGCCCTTCGGCACCTCTAT
  GTGCTAGCTGCAGAACCAAGGCTCCTGGTACCTGTGGATGTGGATACAAACACACCCTGCTATGCCCTTA
  TAGAAGTTACTTACAAGGGCACTCAGTGGTATGAACAGACCAAAGAAGAACTGATGGCTCCAACCCTTCT
  TCCAGAACTTCATCTTTTAAAGCAGATGAAAGTTAAAGGGCCAAGATACTGGGAACTGCTCATAGATTTA
  AGCAAGGGAGAACAGCACTTGAGGTCTATTCTTTCGAAGGATGGAGTTTTATATGTAAAGCTCAGGGCAG
  GACAGCTCTCCTACAAAGAAGACCCAATGGGGTGGCAGAGTTTGTTGGCACAAACTGTTGCTAATAGGAA
  CTCTGAAGCCCGGGCTTTCAAGCCTGAAACAATTTCATCATTCACTTCTGATCCAGCACTTCTGTCATTT
  GCTGAATATTTCTGCAAGCCGACTGTGAGCATGGGTCCAAAACAGGAGATTCTGGACCTCTTTTCTTCGA
  TACTTTATGAGTGTGTTGCTCAGGAAACCCCAGAGATGTTGCCTGCCTACATAGCAATGGATCAGGCTCT
  AAGAAGTCTTAAAAAGAGAGACATGTCAGACACATCTGATCTTTGGCAGATAAAGTTGATATTAGAATTT
  TTCAGTTCCCGAAGCCACCAGGATAGGCAGCATACCTATCCTAAGCGAGGGCTCTTCATAAATTCTGAGT
  TCCTACCTGTGGTGAAGTGCACTGTTGATGCTACCCTGGACCAGTGGCTACAAGCTGGCGGTGATGTGTG
  TGTGCATGCCTACCTCAGTGGGCAGCCTGTTGAGAAGTCTCAGCTAAACATGCTGGCCTGCTTCCTTGTC
  TACCACTCTGTGCCAGCACCACGACACTTGCCCCCCATGGGACTGGAAGGGAGCACAAGCTTTGCTGAAC
  TCCTCTACAGGTTCCGACACCTGAAGATGCCCGTCCGGGCTTTGCTAAGACTGGCTCCTGTGCTGCTTGG
  GAACCCACAGCCGATGGTCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008569
• Insert Size: 5835 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008569.2, NP_032595.2
• RefSeq Size: 8967 bp
• RefSeq ORF: 5835 bp
• Locus ID: 17222
• UniProt ID: P53995
• Cytogenetics: 2 F1
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]