Myh3 (NM_001099635) Mouse Untagged Clone

CAT#: MC225061

Myh3 (untagged) - Mouse myosin, heavy polypeptide 3, skeletal muscle, embryonic (Myh3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myh3
• Synonyms: MyHC-emb; Myhs; Myhs-; Myhs-e; Myhse
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225061 representing NM_001099635
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAGCGACACCGAGATGGAAGTGTTTGGCATAGCTGCACCTTTCCTCCGCAAGTCGGAAAAGGAGA
  GGATCGAAGCTCAGAACCAGCCCTTTGATGCCAAAACCTACTGCTTTGTGGTTGACTCAAAGGAAGAATA
  TGTCAAGGGGAAAATTAAGAGTAGCCAGGATGGGAAAGTCACTGTGGAGACAGAAGACAGCAGGACCCTG
  GTGGTTAAGCCAGAGGACGTGTATGCCATGAACCCACCCAAGTTTGACAAGATTGAGGACATGGCCATGC
  TGACCCACCTGAACGAGCCCGCTGTGCTGTACAACCTCAAGGACCGATACACGTCCTGGATGATCTACAC
  CTATTCAGGCCTCTTCTGTGTCACTGTCAACCCCTACAAATGGCTGCCAGTGTACAACCCCGAGGTGGTG
  GACGGCTACCGAGGCAAAAAACGCCAGGAGGCTCCGCCCCACATCTTCTCCATCTCCGACAACGCCTACC
  AGTTCATGCTGACTGATCGTGAGAACCAGTCTATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACGGT
  GAACACCAAGCGGGTCATCCAGTACTTTGCAACAATTGCAGCCACTGGGGACCTTGCCAAGAAGAAGGAC
  TCCAAAATGAAGGGGACGCTGGAGGATCAAATTATCAGCGCTAACCCCCTGCTGGAGGCCTTTGGGAACG
  CCAAGACCGTGAGGAACGACAACTCGTCTCGCTTTGGCAAGTTCATCCGCATCCATTTTGGCACCACTGG
  GAAGCTGGCTTCTGCAGATATTGAAACTTATCTGCTGGAAAAGTCCAGAGTCACCTTCCAGCTGAAGGCT
  GAGAGAAGCTACCACATCTTCTACCAGATTCTCTCCAACAAGAAACCTGAGCTGATTGAGCTGCTGCTGA
  TTACGACCAACCCTTATGACTACCCGTTCATCAGCCAGGGCGAAATCCTGGTGGCCAGCATCGACGATGC
  GGAGGAGCTGTTAGCTACGGATAGTGCCATCGACATCCTGGGCTTCACCCCGGAGGAGAAATCTGGACTT
  TATAAACTGACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG
  CCGAGCCAGATGGCACTGAAGTGGCTGACAAAACAGCCTACTTGATGGGCCTGAATTCCTCAGACCTCCT
  CAAGGCCTTGTGCTTTCCCAGAGTGAAAGTCGGGAATGAATACGTTACAAAAGGGCAGACTGTGGACCAG
  GTTCACCATGCTGTGAACGCTCTCTCCAAGTCCGTGTACGAGAAGCTGTTCTTGTGGATGGTCACTCGTA
  TTAACCAGCAGCTGGATACAAAGCTACCAAGACAGCACTTCATTGGCGTTTTGGACATTGCGGGTTTTGA
  AATTTTTGAGTATAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAGTTTTTC
  AACCACCACATGTTCGTGCTGGAACAGGAGGAATACAAGAAGGAGGGCATCGAGTGGACCTTCATCGACT
  TCGGGATGGACCTGGCAGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCTGGAAGA
  GGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTGGGAAAG
  TCCAACAACTTCCAGAAACCTAAGGTGGTCAAAGGCAAGGCTGAAGCCCACTTCTCCCTGGTGCACTATG
  CGGGCACCGTGGACTACAGTGTCTCAGGCTGGCTGGAGAAGAACAAGGACCCCCTGAATGAGACCGTGGT
  GGGACTGTACCAGAAGTCCTCCAACAGACTCCTGGCACATCTCTATGCCACCTTCGCTACAACAGATGCG
  GACGGTGGGAAGAAGAAAGTCGCTAAGAAGAAAGGCTCTTCCTTCCAAACCGTCTCTGCACTGTTCAGGG
  AAAACTTGAACAAGCTGATGTCCAATTTAAGAACCACACACCCACACTTTGTACGCTGTATAATACCCAA
  CGAAACCAAGACCCCAGGGGCCATGGAACACAGCCTTGTCCTGCACCAGCTCAGGTGTAACGGGGTGCTG
  GAAGGCATCCGGATCTGCAGGAAGGGCTTCCCCAACAGGATCCTGTATGGAGACTTTAAACAGAGATACC
  GAGTGCTGAACGCCAGTGCCATCCCAGAAGGCCAGTTCATTGACAGCAAGAAAGCTTGCGAGAAACTCTT
  GGCTTCCATTGACATCGACCACACACAGTACAAATTTGGACACACCAAGGTGTTCTTTAAGGCTGGCTTG
  CTAGGAACCCTGGAAGAGATGCGGGATGAGCGTTTGGCCAAACTGATCACTCGGACACAAGCCGTGTGCA
  GAGGGTTTCTCATGCGTGTGGAATTCCAGAAGATGATGCAGAGGAGGGAGTCCATCTTCTGCATCCAGTA
  CAACATCCGTGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCCCTG
  CTGAAGAGCGCGGAGACTGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGGATGAGC
  TGGCTAAGTCGGAGGCAAAGAGGAAGGAGCTAGAGGAGAAGCTCGTCACTTTGGTACAGGAGAAGAATGA
  CCTGCAACTTCAAGTACAGGCTGAGAGTGAAAATTTGTTGGATGCTGAGGAACGATGCGACCAGCTGATC
  AAAGCCAAGTTCCAGCTGGAAGCCAAAATCAAAGAGGTGACAGAGAGAGCCGAGGACGAGGAGGAGATCA
  ACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCGGAGCTGAAGAAGGACATCGATGA
  CCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACAGAGAACAAGGTCAAAAACCTTACC
  GAAGAACTGGCAGGCCTGGATGAAACTATTGCAAAGCTAACACGGGAGAAGAAGGCCCTCCAAGAGGCCC
  ACCAGCAGACCCTGGACGACCTCCAGGCTGAAGAAGACAAGGTCAATTCTCTGAGCAAGCTCAAGAGCAA
  GCTGGAGCAGCAAGTGGATGATCTGGAGAGCTCCCTCGAACAAGAAAAGAAGCTGCGTGTGGACCTAGAA
  AGGAACAAAAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACA
  AGCAACAGCTAGACGAGAGGCTCAAGAAGAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGA
  TGAGCAGACCTTGAGCCTCCAGCTTCAGAAGAAAATCAAGGAGCTACAGGCTCGCATCGAGGAGCTGGAG
  GAAGAGATAGAGGCAGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGC
  TGGAGGAGCTGAGTGAGCGGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAA
  GCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTG
  GCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGG
  TGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGA
  GAGCGTGTCCAAATCCAAGGCCAATCTGGAGAAGATCTGCCGAACTCTAGAGGATCAGTTAAGTGAGGCC
  AGGGGCAAGAATGAGGAGATGCAGAGGAGCCTAAGTGAGCTGACCACACAGAAGTCCCGGCTGCAGACAG
  AGGCGGGTGAGCTGAGTCGTCAACTAGAAGAAAAGGAAAGCATTGTATCTCAGCTCTCCAGGAGCAAGCA
  GGCCTTTACCCAACAGATAGAGGAGCTCAAGAGGCAACTGGAGGAAGAGAACAAGGCCAAGAACGCGCTG
  GCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTACGAGGAGGAGCAGGAAG
  GCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAAGTATGA
  AACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAAAAAAAGCTTGCGCAGCGCCTTCAGGAT
  TCGGAGGAGCAGGTAGAGGCTGTAAATGCCAAATGTGCTTCCTTGGAAAAGACCAAGCAGAGGCTGCAAG
  GGGAGGTGGAGGACCTGATGGTGGATGTGGAAAGAGCCAACTCCCTGGCTGCCGCTCTGGACAAGAAGCA
  GAGGAACTTTGACAAGGTGCTAGCTGAGTGGAAGACGAAGTGTGAGGAGAGCCAAGCCGAGCTGGAGGCA
  GCTCTCAAGGAATCCCGGTCCTTGAGCACTGAGCTCTTCAAACTGAAGAATGCCTATGAAGAAGCCTTGG
  ATCAACTTGAAACCGTGAAACGGGAGAATAAGAACTTAGAACAAGAGATAGCAGATCTCACTGAACAGAT
  TGCCGAGAACGGTAAAAGCATCCATGAGCTAGAGAAATCCAGAAAACAGATGGAGCTGGAGAAGGCTGAC
  ATCCAGATGGCGCTGGAGGAAGCAGAGGCAGCTCTTGAGCACGAAGAAGCCAAGATTCTCCGGATCCAGC
  TTGAACTGACCCAGGTGAAATCAGAGATTGATAGGAAGATTGCAGAGAAGGATGAAGAGATCGAGCAGCT
  GAAGAGGAACTACCAGAGGACAGTGGAGACCATGCAAGGCGCCCTGGACGCTGAGGTGCGCAGCAGGAAT
  GAGGCCATCCGCCTGAAGAAGAAGATGGAGGGCGACCTCAATGAGATCGAGATCCAGCTGAGCCACGCCA
  ACCGCCAGGCCGCAGAGACCATCAAGCACCTCCGGAGCGTCCAGGGACAGCTGAAGGACACCCAGCTGCA
  CCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATCGTGGAACGCAGAGCCAAC
  CTCCTGCAGGCTGAGGTGGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGAGGGCCCGGAAGCTGGCAG
  AGCAGGAGCTGTTGGATTCGAATGAGAGGGTCCAGCTGCTGCACACCCAGAACACAAGCCTCATCCACAC
  CAAGAAGAAGTTGGAGACTGACCTCACGCAGCTCCAGAGTGAGGTGGAGGATGCCTGCAGGGACGCAAGG
  AATGCAGAGGAGAAGGCCAAAAAGGCCATCACTGACGCCGCCATGATGGCGGAGGAGCTGAAGAAGGAGC
  AGGACACCAGCGCCCACCTGGAGAGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCAGCACCG
  TCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATTCAGAAACTGGAGACACGGATC
  AGAGAGCTGGAGTTTGAGCTGGAAGGGGAGCAGAAGAGGAACACAGAGTCTGTGAAGGGCCTGAGGAAGT
  ATGAGCGGCGTGTTAAGGAGCTCACATATCAGAGTGAGGAGGACAGGAAGAATGTGTTGAGATTGCAGGA
  TCTGGTGGATAAACTCCAAGTGAAAGTCAAGTCCTACAAGAGGCAGGCTGAGGAGGCTGATGAACAAGCC
  AATGCTCATCTCACCAAGTTCAGGAAAGCCCAGCATGAGCTAGAGGAGGCCGAGGAACGGGCTGATATCG
  CAGAATCGCAAGTCAATAAACTGCGGGCAAAGACCCGTGACTTCACCTCTAGCCGGATGGTGGTCCATGA
  AAGCGAGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001099635
• Insert Size: 5823 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099635.1, NP_001093105.1
• RefSeq Size: 5992 bp
• RefSeq ORF: 5823 bp
• Locus ID: 17883
• UniProt ID: P13541
• Cytogenetics: 11 40.59 cM
• Gene Summary: Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. [provided by RefSeq, Sep 2015]