Mia3 (NM_177389) Mouse Untagged Clone

CAT#: MC225054

Mia3 (untagged) - Mouse melanoma inhibitory activity 3 (Mia3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mia3
• Synonyms: 9130229H14Rik; A930039G15Rik; AU022327; B230399H06Rik; C80126; Gm1525; Tango; TANGO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225054 representing NM_177389
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCGCCGGGGCTGCTCTTCTGGCTGTTCGTGCTGGGGGCGCTCTGGTGGGTCCCGGGCCAGT
  CGGATCTCAGCCACGGACGGCGTTTCTCGGACCTCAAAGTGTGCGGGGACGAAGAGTGCAGCATGTTAAT
  GTACCGTGGGAAAGCTCTTGAAGACTTCACGGGCCCTGATTGTCGTTTTGTGAATTTTAAAAAAGGTGAC
  GATGTATATGTCTACTACAAACTGGCAGGGGGATCCCTTGAACTTTGGGCTGGAAGTGTTGAACACAGTT
  TTGGATATTTTCCAAAAGATTTGATCAAGGTACTTCATAAATACACGGAAGAAGAGCTACATATTCCAGC
  AGATGAGACAGACTTTGTCTGCTTTGAAGGAGGAAGAGATGATTTTAATAGTTATAATGTAGAAGAGCTT
  TTAGGATCTTTGGAACTGGAGGACTCTGTACCTGAAGAGTCGAAGAAAGCTGAAGAAGTTTCTCAGCACA
  GAGAGAAATCTCCTGAGGAGTCTCGGGGGCGTGAACTTGACCCTGTGCCTGAGCCCGAGGCATTCAGAGC
  TGATTCAGAGGATGGAGAAGGTGCTTTCTCAGAGAGCACCGAGGGGCTGCAGGGACAGCCCTCAGCTCAG
  GAGAGCCACCCTCACACCAGCGGTCCTGCGGCTAACGCTCAGGGAGTGCAGTCTTCGTTGGACACTTTTG
  AAGAAATTCTGCACGATAAATTGAAAGTGCCGGGAAGCGAAAGCAGAACTGGCAATAGTTCTCCTGCCTC
  GGTGGAGCGGGAGAAGACAGATGCTTACAAAGTCCTGAAAACAGAAATGAGTCTTGACCTGAAAACCAAG
  TTTGGCTCAACTGCCGACGCCCTTGTGTCTGACGACGAGGCAACCAGACTGGTCACTTCATTAGAAGACG
  GCTTCGATGAAGCGCTGGATGCTGAGTATTACCCGATGGAGGAGGAGGAGGAGGTAGAGGAGGATGCAGA
  CAGCTCTGATGAGCTACCATTACTAACCTTCTCAGACAAAGATGAGAAAGTCCCCGGGAAGCCCATGATA
  GAGAAATATTTAACAGATAAGGATCCGAATTTGAGTGAAGAGGATAAAGTTGAACCCCCTACCTGGGGGG
  ATGCTTTTTTTTCTATTGTCACAGGAGGTGAAGGGAAACCAGGTGTGGTGGATTTAGAAAGATCTATCGA
  GGAGGAGGAAGATGTGTCAGTTTCATCTAGCCATCAGAGGAAGCCACAGCCAGCAGCTGGCTATACAGAC
  AGTGAGGATGAAGGTGACGATCTTTTTGTAGAAGAGCCCAAAACAAATGATGTAAAGGACTCAGAAACAG
  ACCCAGAACTTGTCATTACAGGAGAAGAAAAGGACATCCAGGAATCCAGGAAAGGCCTGGTACAGCCTGA
  GTCACAATCAGAGGATGCCAAGTCAGAAACGGCGAGCGCTTATCGTCTTCAGGGTAGCAAGCTCAACCCT
  CTGTCAGCTGCTGAAAAGGGCAGAGACTTTACACTAAAAGCCGTCTTTGAAAAAAAAGAAAATGGCCTAA
  AAGAATCAGTTATCCATATCTCAAAGGAAACACTACATGAAGACAAGACCAGAGAGATCCAGAGAGACAG
  CTTGGAGAGTGAATTGGTACACAGAGCTTTAGGGAGTTCAGTGACAGAAAATAACAAGCCCAAATCCTTG
  GGTGTTGCACCACTCTTGGGAAATAACAAGCCCGATGCATCCAAAGACAGTACAGAGGTCCCAGACGGTT
  CCGTCAGTGGACCAAAAGCAGGCCAGCAGGAGGGATTTCTAGAACCAGGTCTTAAAACTCAGCACCAACC
  TAGATTCTCCCCTCCAGAGGAAACTGGTCCCTCAAGAGAACTGGGGGGAAAAGTTCCTATTTCGGGAAGA
  AATCTTTCTTGGCAGCAAGAGCAAGACGTAGCTGCTGTAGTCGGTAAGCATGCGAATGAGAAGACAGGGT
  TTCCGGAGGAAGAGAGCAGGGAAGACGGGACAGATGCGGAGCAAGCCAGGGCCATACGAAGGCCACAGGA
  GGCTGAGAGCCCGGAAGTGCTGAGTGTCCAACCTGGAAGGCCAGACGAGGAGGAGGAGGAGGAAGAGGGC
  GACAACTATCCCCCCGAGGGGCTGATGGAGGATGAAAATGCTGTGAGCGCACAGCAGTCTAGAGAAAATT
  CCCCCAGCGCTCGCGACGGGAGGTCAGATATGAATTCTCAAGTTTTTGAAAAAGTAATTTTGGGGACCCT
  TAATCTTAACACTGAAAAAACCAAACAACCAGCTAATATGATTTTGGAGACTGGACAAGAGAGTGAAACC
  ACATCCGAAGAGGCAGGTGACGTGGGCAAGGAGTCAGGTCACAGTGTGGTGGTGGACAGTGAAGAAAGCC
  ATCTGGCAGATATGAGAGCCCAGAGACCCTCTCAAGTCCACGGCCTTCGTGACGAGACAGCAGCTCAGAC
  TCCCGGCTCCGGGGAAGCTGTTCTGAGTAAAAATCCTAATGACCTACAAAAAGACAACCCAGAGGAAGAA
  CTGGTCAACACCTTGGGACTTGAAGACCCTGGCGTAGGAGAAATCTCAGAAGGGGAGCCGGAAGATACCA
  AGGAGTTTGGAGTCTCAGAAAGCCAGGGGACTGATGCTGAAGACCTTAGGGATGACCCATCCCGGCAAGC
  TACACCGGAAATTCCTGACATAGTGCTCAAGAGTATAAGGGAAGACTTGCCTATCATAAACAGCTTCTTC
  AAGGACGACCAACAGTCCCTGCATCGTTTCCTGAAGTACTTCGATGTTCGTGAGCTGGAGGGCTTGCTTG
  AAGACATGTCGATAAGGCTGAGATCAGCGCACCAGAACAGCCTGCCGTACAACATGGAGAAAGTCCTAGA
  TAAAGTCTTCCGTGCCTCTGAGTCACGAATTCTAAGTATGGCGGAGAAAATGCTTGATACGGGAGTGGCC
  AAAAATAGGGACCTGGGCTCCAAAGAGAGCAGTCCGTTGGAAGAAGCCGAGGTGTTGGATGACATACAAG
  ACCTGATCTATTTTGTCAGGTACCAATACTCTGGAGTGGAGACTGCCCCATTAGTCACACCTCCACCTCC
  GGAGGAAGGCTGGGCCAGACCAGGAGAAGAGAGACAGCCACCCCAGCAAGACAGCTTACCACAAGAAAAC
  ACAGGAGACCTCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACCAGTGTGCAGC
  CTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTC
  GGATCAACCTGTGACCAGTGTGCAGCCTCCAGAAGAGCCTGAGCTCTCGGATCAACCTGTGACTGGGTAC
  ACAAGTACCTCAGAGGTATCACAGAAGCCAGATACCAAGAAAGACATAGATCTAGGTCCTGTAATGGAAG
  GTGGTCCTGTTGGGGCTGGAGATGTACAAAAGCAACTAGAGACAATTGCAGAGGAGCCAGCAGCTGTCCC
  ACCTCTGGAAAGTGCATTTGGTTCCCTCTATGCCTTCATCCTTTATTTAAGTAAGATGCTCCTTGCCACG
  TTGCCTGACAATGTTCAGCCTGGCCCTGACTTTTACGGACTGCCCTGGCAACCTGTGATCATTACTGCAG
  TCTTGGGAATTGTTTCATTTGCAATTTTCTCCTGGAGAACTATTCTTGTTGTAAAGAGTAGAGTATATCA
  AGTTACTGAGAAACAAATTTCTGAAAAGTTGGAGAATATCAAGAAAGAAAATGCAGAACTTATGCAAAAA
  TTGTCAAGTTATGAACAAAAGATCAAGGAGTCAAAGAAGTATGTTCAAGAAACCAAGAAACAAAACATGA
  TTCTCTCCGATGAAGCTGTTAAATATAAGGATAAAATCAAGATCCTTGAAGAAACTAATGTAAGTTTGGG
  TGACAAAGCCAAAAGCCTTCGTCTTCAGTTAGAGTCTGAGAGGGAGCAGAACGTCAAGAACCAGGACCTG
  ATACTGGAAAACAAGAAATCTATTGAGAAGTTGAAGGATGTCATCTCAATGAATGCATCAGAACTTTCAG
  AGGTTCAAGTTGCCCTTAATGAAGCCAAGCTCAGTGAAGAGAATGTAAAATCTGAGTGCCATCGGGTTCA
  AGAAGAAAATGCCAGGCTTAAGAAGAAGAAAGAGCAGTTGCAGCAGCAGGTAGAAGAGTGGAGTAAATCG
  CATGCCGAGCTCACTGGACAGATCAAGTCATTTGAGAAGTCCCAGGAAGACTTGGAAATAGCTCTGACTC
  ACAAAGACGACAATATTAGTGCTTTGACTAATTGCATCACACAGCTGAATCGGTTAGAATGTGAACTTGA
  ATCTGAGGATCCAGATAAAGGAGGAAATGAGTCAGATGATCTGGCCAATGGAGAAACGGGAGGTGACCGG
  AGCGAGAAGATAAGAAATAGAATTAAGCAGATGATGGACGTCTCTCGGACACAAACTGCAGTCTCAATAG
  TTGAAGAGGATCTAAAACTTTTACAGCTTAAGCTAAGAGCATCGATGTCCACTAAATGTAACCTGGAAGA
  CCAAATCAAGAAATTAGAAGATGACCGCAGCTCGCTGCAGACTGCTAAAGCTGGGTTGGAAGACGAGTGC
  AAAACTCTGAGGCAGAAAGTGGAGATCCTGAATGAGCTGTACCAGCAGAAGGAGATGGCGCTGCAGAAGA
  AACTGAGTCAAGAAGAGTATGAGCGGCAAGACAGAGAGCAGAGGCTCACGGCGGCCGATGAGAAGGTGGT
  TCTGGCTGCAGAGGAAGTGAAAACGTACAAGCGGCGAATCGAAGAAATGGAAGAAGAATTACAGAAAACA
  GAACGTTCATTTAAAAACCAGATTGCTGCTCATGAGAAGAAAGCTCATGATAATTGGCTCAAAGCTCGTG
  CTGCAGAGAGAGCCATGGCAGAGGAGAAGAGAGAAGCTGCTAACTTAAGGCACAAATTACTGGAAATGAC
  TCAAAAGATGGCAATGAGGCAAGATGAGCCCGTGATTGTAAAGCCGATGCCAGGAAGACCGAACACACAG
  AACCCTCCCCGGCGAGGTCTGCTGAGCCAGAATGGCTCTTTTGGCCCATCCCCTGTGAGTGGTGGGGAAT
  GCTCCCCTCCCCTACCAGCAGAGCCGCCTGGGAGACCTCTCTCTGCCACACTCAGTCGAAGAGACACTCC
  TAGAAGTGAATTTGGGTCACTGGACAGGCATTTACCTCGCCCTCGATGGCCATCAGAGGCATCTGGCAAA
  CACTCTGCTTCTGACCCGGGTCCCGCTCCTGTGGTGAACAGCAGCTCCAGGAGCTCTTCTCCCGCTAAGG
  CCGTGGATGAGGGCAAGGTTAATATGGCTCCCAAAGGGCCGCCTCCATTTCCAGGGGTGCCTCTCATGGG
  AGGCCCAGTGCCACCACCCATACGATATGGACCACCTCCCCAGCTATGTGGTGGGCCTTTTGGGCCTCGC
  CCACTTCCTCCACCATTTGTTCCAGGCATGCATCCACCACTAGGCGTAAGAGAATATGCACCAGGTGTTC
  TGCCTGGGAAACGGGACCTGCCTCTTGATCCTCGGGAATTTTTACCAGGACATACACCATTTAGACCTCC
  AGGTTCACTCGGTCCAAGAGAGTTCTTTATTCCTGGTACCCGATTACCACCTCCAACCCATGGTCCTCAG
  GAATACCCACCACCACCACCTGCTGTAAGAGACTCACTGCCTTCAGGCCCGAGAGAGGAAGCCAAACCTG
  CCTCTCCAAGCAGTGTCCAGGACCGCTCACAGGCTTCAAAGCCCACCCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177389
• Insert Size: 5793 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177389.3, NP_796363.2
• RefSeq Size: 6933 bp
• RefSeq ORF: 5793 bp
• Locus ID: 338366
• UniProt ID: Q8BI84
• Cytogenetics: 1 H5
• Gene Summary: Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum. Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES.[UniProtKB/Swiss-Prot Function]