Pcdh15 (NM_001142740) Mouse Untagged Clone

CAT#: MC225049

Pcdh15 (untagged) - Mouse protocadherin 15 (Pcdh15), transcript variant E, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcdh15
• Synonyms: av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225049 representing NM_001142740
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA
  GCTGGGGCCAGTATGACGATGATTGCAAACTAGCTAGGGGAGGACCACCAGCTACTATCGTGGCCATTGA
  TGAAGAGAGTCGAAACGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACTGCCGGAGGACCAGAC
  CCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACCCCGTTAAACAGATGC
  TTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACACTCCATTGTGGTGCA
  AGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATCGTGGTGCGAGATCGG
  AATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGCTCACTCCAGTTGGCA
  CCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGGCCCTAATGGACAGAT
  AGAATACGTGATTCAGTACAACCCAGAAGATCCGACATCCAACGACACCTTTGAAATTCCACTCATGCTG
  ACTGGCAACGTGGTACTGAGGAAAAGACTCAACTATGAGGATAAGACTCGCTACTATGTCATCATCCAAG
  CAAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACCACCACCACCCTCACAGTAGATGTTCTAGA
  TGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTGTGCCAAACACACGTGACTGTCGTCCACTC
  ACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTTTGGTGACACCACCTA
  TCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCATCCTCTACTCCATCCT
  TGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGCATCCCAGGACTGCAGAACTCACTCTCCTG
  GAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGTTATTAAGGCTGAGCAGGACAATGGCCACC
  CACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGACGAAAACAATCAGAGTCCATACTTCACAAT
  GCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGGGAGCCACCATTTCTGAGAGCCTAAACTTA
  ACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACACAAAAGATCCAGAGCTCCACCTCT
  TCCTGAATGACTACACCTCGGTCTTCACTGTGACACCCACTGGTATCACCCGCTACCTCACCCTGCTTCA
  ACCTGTGGACAGGGAGGAACAGCAAACCTACACCTTTCTGATAACAGCGTTTGATGGCGTGCAAGAAAGT
  GAGCCAGTCGTGGTCAATATCCGAGTGATGGATGCAAATGATAACACGCCCACCTTCCCTGAAATCTCCT
  ATGATGTCTATGTTTACACAGACATGAGTCCTGGGGACAGCGTCATTCAGCTGACAGCGGTAGATGCTGA
  TGAAGGCTCTAATGGGGAGATCTCCTATGAAATACTGGTGGGGGGCAAGGGAGACTTCGTGATCAACAAG
  ACCACAGGGCTGGTGAGCATTGCACCAGGCGTGGAGCTGATCGTGGGACAGACGTATGCGCTCACAGTGC
  AGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAGGCACTCCATCTGCACAGTGTACATCGAGGTGCTTCC
  TCCTAACAACCAGAGCCCTCCCCGCTTCCCGCAGCTGATGTACAGTCTGGAAGTCAGCGAGGCCATGAGG
  ATCGGTGCTATTTTATTAAATCTACAGGCAACTGATCGAGAGGGAGATCCAATCACATATGCCATCGAGA
  ATGGAGACCCTCAGAGAGTTTTTAATCTTTCAGAAACCACAGGGATTCTCAGCCTAGGGAAGGCTCTAGA
  CCGCGAGAGCACAGACCGCTACATCCTCATCGTCACAGCCTCAGATGGCAGACCGGATGGAACCTCAACT
  GCCACTGTGAACATAGTGGTGACGGACGTCAATGACAACGCTCCCGTGTTCGATCCCTATCTGCCCAGGA
  ACCTCTCTGTGGTGGAGGAAGAAGCCAATGCCTTTGTGGGTCAAGTCCGGGCAACAGACCCAGATGCTGG
  GATAAACGGCCAAGTTCACTACAGCCTGGGGAACTTCAACAACCTCTTCCGCATCACATCCAACGGGAGC
  ATTTACACAGCCGTGAAGCTGAACAGGGAAGCCAGGGACCACTATGAACTGGTTGTCGTGGCAACAGATG
  GAGCAGTCCACCCTCGACATTCAACTCTGACACTGTACATCAAGGTGTTGGACATTGATGATAACAGTCC
  TGTTTTTACCAATTCAACGTACACAGTTGTCGTTGAAGAGAATCTGCCAGCCGGGACCTCCTTTCTTCAA
  ATAGAGGCCAAGGATGTTGACCTTGGAGCCAATGTGTCATATCGGATCAGAAGCCCAGAAGTGAAACACC
  TTTTTGCACTGCATCCATTCACTGGAGAATTGTCTCTTCTGAGGAGTTTGGATTATGAGGCCTTTCCGGA
  CCAGGAGGCAAGCATCACATTCTTGGTGGAGGCCTTTGACATTTATGGGACTATGCCACCTGGTATAGCA
  ACAGTCACGGTAATTGTGAAGGACATGAATGACTACCCTCCAGTGTTTAGCAAACGCATCTACAAGGGGA
  TGGTGGCTCCAGATGCAGTCAAGGGGACACCAATCACCACCGTTTATGCTGAAGATGCGGACCCACCTGG
  GATGCCTGCAAGTAGGGTGAGGTATCGAGTGGACGACGTGCAGTTTCCATACCCAGCCAGTATTTTTGAT
  GTAGAGGAAGATTCTGGAAGAGTAGTAACCCGCGTCAATCTTAATGAAGAGCCTACTACGATTTTCAAGC
  TGGTGGTTGTGGCTTTTGATGACGGCGAACCTGTGATGTCCAGCAGTGCCACGGTGAGAATTCTTGTCTT
  ACATCCTGGAGAGATCCCACGCTTCACCCAAGAGGAATACAGACCTCCTCCTGTAAGTGAGCTTGCGGCC
  AGAGGGACTGTAGTTGGTGTCATTTCTGCTGCTGCCATTAATCAGAGCATCGTGTACTCCATTGTGGCAG
  GAAATGAGGAAGACAAGTTTGGAATCAACAATGTCACTGGGGTCATCTATGTGAATTCACCATTGGATTA
  CGAGACAAGGACCAGCTATGTGCTCCGGGTACAAGCAGATTCTCTGGAAGTGGTCCTTGCCAATCTCCGA
  GTCCCTTCAAAAAGCAATACAGCTAAGGTGTACATTGAGATTCAGGATGAAAACGATCACCCCCCAGTGT
  TCCAGAAGAAATTCTACATTGGAGGTGTGTCTGAAGACGCAAGGATGTTCGCATCTGTGCTCAGAGTGAA
  GGCCACCGACAGGGACACGGGTAATTACAGTGCCATGGCCTACCGGCTCATCATACCGCCGATTAAAGAG
  GGCAAAGAGGGGTTTGTGGTGGAAACATACACAGGTCTCATCAAGACAGCCATGCTCTTCCACAATATGA
  GAAGATCCTACTTCAAGTTTCAAGTGATTGCAACTGACGACTACGGGAAGGGGTTGAGCGGGAAAGCAGA
  CGTACTGGTCTCCGTGGTCAATCAACTGGATATGCAGGTCATTGTCTCCAATGTGCCCCCTACACTAGTG
  GAAAAGAAGATAGAAGACCTTACAGAGATTTTGGATCGCTACGTTCAGGAGCAAATTCCTGGTGCCAAGG
  TTGTGGTGGAGTCCATAGGTGCCCGTCGCCATGGAGACGCCTACTCCCTAGAAGACTATAGCAAGTGCGA
  CCTGACTGTCTATGCCATCGACCCGCAGACCAACAGAGCCATCGACAGAAATGAGCTTTTTAAGTTCCTG
  GACGGCAAACTGCTCGATATCAATAAAGACTTCCAGCCGTATTACGGGGAAGGAGGGCGCATTCTGGAGA
  TTCGGACACCTGAGGCAGTGACGAGCATCAAGAAGCGAGGAGAAAGCTTGGGGTACACAGAAGGGGCCTT
  GCTGGCCTTGGCCTTCATCATCATCCTCTGTTGCATCCCAGCCATCTTGGTCGTCTTAGTAAGCTACCGA
  CAACGCCAGGCTGAGTGCACGAAGACCGCAAGAATTCAGTCTGCTATGCCTGCAGCCAAGCCTGCAGCTC
  CTGTACCAGCTGCGCCTGCGCCGCCCCCGCCCCCGCCACCACCACCACCAGGAGCACATCTCTATGAAGA
  ACTGGGAGAGAGCGCAATTCTTTTCCTTCTCTACCATTTTGAACAAAGCAGGGGAAATAACTCAGTCCCA
  GAAGACAGGAGCAGTCATCGCGATGGGATGGCCTTTTCCTCCAGTACCACTGAGTCTCATGAGCCAGCTC
  ATGTAGAGGGACCACTTAAGGAGAGCCAGCCTAACCCAGCAAGGACGTTCTCATTTGTTCCTGATGAGGA
  TAACTTAAGTACCCATAATCCCCTTTACATGGAAAGTATAGGTCAAAGGTCAACAAACTCAGACCTTCAG
  CCACGAACAGATTTTGAAGAGCTGTTGGCACCCAGAACACAAGTTAAGAGTCAGTCTCTGAGGGGCCCAA
  GAGAAAAGATCCAGAGGGTGTGGAATCAGTCTGTGAGCTTTCCTAGGCGGCTCATGTGGAAAGCCCCAAA
  CAGGCCAGAGACCATAGACCTGGTGGAGTGGCAGATCACCAATCAGAGAGCTGAATGCGAAAGCGCCAGA
  TGCCACCCAAGCCAGAGAGGTAGCAGCAACGTTCTGCTGGCAACTGAAGATGCCCACGAGTCAGAGAAAG
  AAGGGGGACACAGAGACACCCTAATCGTCCAGCAAACAGAGCAGCTGAAATCTCTGTCTTCTGGCTCTTC
  TTTTTCCTCCTCTTGGTCTCACTTTTCTTTCTCAACTCTGCCAACGATTTCCAGAGCGGTGGAACTCGGG
  TCGGAACCTAATGTGGTCACTTCTCCCGCTGACTGCACCTTGGAACTTTCTCCTCCTCTGAGACCCCGTA
  TTTTAAACTCCTTAAGCTCTAAGAGAGAGACTCCCACATGTGCATCAGATACAGAACCAAAAAGGAACTC
  TTTTGAGATCGCTCCCCATCCACCTAGCATCTCTGCTCCCCTCCCACATCCGCCTCTTCCTAGACCTCCC
  ATTGCCTTTACCACTTTTCCTCTTCCCCTTTCTCCCCCTAACCCTCCTCCCCCACAACTTGTTACATTTT
  CTCTTCCCATTTCTACACCCCCTACTTCTTCTCTACCTCTTCCTCCTCCACTGTCACTTCCTCCTCCTCC
  TCGGCCACCAGCTCCCCGCCTCTTCCCACAGCCTCCTTCCACGTCCATTCCATCCACAGACAGCATCTCT
  GCACCAGCTGCTAAATGCACTGCCAGTGCCACACACGCCAGAGAAACCACGTCTACGACACAGCCACCAG
  CATCCAACCCGCAGTGGGGGGCAGAACCCCACAGACATCCAAAAGGGATCCTCAGACATGTGAAAAACTT
  GGCAGAGCTCGAGAAATCAGTGTCTAACATGTACAGTCACATAGAAAAAAACTGCCCACCTGCAGATCCC
  TCAAAACTACACACGTTTTGCCCTGCAGAGAAAACAGGCATGAAAATCACACATGACCAGAGCCAGGAAA
  CGTTGGTTAGAGTTGTTGAGGGAATTGACGTGCAACCTCACAGTCAATCAACATCTTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142740
• Insert Size: 5802 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142740.1, NP_001136212.1
• RefSeq Size: 9034 bp
• RefSeq ORF: 5802 bp
• Locus ID: 11994
• UniProt ID: Q99PJ1
• Cytogenetics: 10 37.43 cM
• Gene Summary: Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (E) lacks three alternate in-frame exons, compared to variant A. The resulting isoform (CD1-7), also known as protocadherin-15-CD1 isoform 7, has the same N- and C-termini but lacks three internal segments, compared to isoform CD1-1.