Sbf2 (NM_177324) Mouse Untagged Clone

CAT#: MC224997

Sbf2 (untagged) - Mouse SET binding factor 2 (Sbf2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sbf2
• Synonyms: 4833411B01Rik; AA414977; AI317167; B430219L04Rik; mMTMH1; Mtmr13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224997 representing NM_177324
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGCTGGCCGACTACTTCATTGTGGTAGGCTACGACCACGAGAAGCCAGCAGGGCCAGGAGAAG
  GTCTGGGGAAAATAATTCAGAGATTCCCACAGCAGGACTGGGATGACACGCCTTTCCCACAGGGAATTGA
  ATTGTTTTGTCAACCTGGTGGATGGCACCTGTCCAGAGAGCGGAAACAGCCAACATTCTTTGTGGTGGTC
  CTAACAGATATTGACTCAGATCGACATTACTGCTCATGTCTAACTTTCTATGAGGCAGAGATCAATCTCC
  AGGGAACGAAGAAGGAAGAGATTGAAGGTGAAGAAGTATCTGGTTTAATTCAACCAGCAGAAGTATTTGC
  CCCCAAAAGCCTGGTGTTGGTATCCAGATTAGACTATCCAGAAATCTTTAGGGCCTGCCTGGGTTTGATC
  TATACTGTGTATGTGGACAGCATGAGTGTCTCCTTGGAAAGTCTCATCGCAAATCTTTGTGCTTGCCTTG
  TCCCAGCTGCTGGAGGGTCTCAGAAGCTATTTTCCTTGGGTGCAGGAGACAGACAACTGATCCAGACACC
  TTTGCATGACAGCCTTCCTGTCACAGGCACTAGTGTGGCTCTTTTATTTCAGCAATTAGGCATTCAAAAC
  GTCCTCAACCTCTTCTGCGCAGTCCTTACAGAAAACAAGGTTCTCTTCCACTCTGCAAGCTTCCAGAGAC
  TCAGTGATGCCTGTAGAGCCCTGGAATCTCTAATGTTTCCTCTGAAGTACAGTTACCCGTATATCCCCAT
  TCTTCCGGCTCAGCTCCTAGAAGTTTTGAGTTCTCCAACTCCGTTCATTATTGGCGTACACTCCATCTTT
  AAAACTGATGTCCATGAGCTTTTAGATGTAATCATAGCAGATTTGGATGGAGGCACTATTAAAATTCCTG
  AATGCATTCACCTCTCATCCCTCCCGGAGCCTCTTCTTCATCAGACTCAATCAGCACTTTCTCTGATCTT
  GCACCCGGATTTGGAAGTAGCAGATCATGCCTTCCCTCCTCCACGAACAGCTTTATCCCACTCAAAAATG
  CTGGATAAAGAAGTTCGAGCAGTTTTCCTTAGGTTATTTGCACAACTGTTCCAAGGATACAGGTCATGCC
  TCCAGCTTATCCGGATCCATGCCGAGCCTGTGATACACTTTCACAAAACAGCTTTCTTGGGGCAGCGTGG
  CTTGGTTGAGAATGATTTCCTCACTAAAGTGCTCAATGGAATGGCATTTGCAGGTTTTGTTTCAGAAAGA
  GGACCTCCCTATAGAGCCTGTGATCTCTTTGATGAGTTGGTAGCTTTTGAAGTAGAAAGAATTAAAGTTG
  AAGAAAAGAATCCACTGAAGATGATAAAGCACATCAGAGAACTTGCTGAGCAGCTATTCAAAAACGAGAA
  TCCAAACCCTCATATGGCATTCCAGAAAGTTCCACGTCCAACAGAAGGATCCCACTTGAGAGTTCATATT
  CTTCCTTTTCCAAAGATTAATGAAGCCCGGGTTCAAGAACTAATACAGGAAAATCTTGCTAAGAACCAGA
  ATGCACCTCCTGCTACACGAATAGAAAAGAAATGTGTAGTGCCAGCAGGTCCACCCGTTGTTTCAATAAT
  GGAAAAAGTGATAACAGTTTTTAACAGTGCACAGAGACTGGAAGTTGTTAGAAACTGCATCTCATTCATA
  TTTGAAAATAAAACTTTGGAAACTGAGAAGACCCTTCCTGCTGCCTTGAGGGCCCTTAAAGGAAAAGCAG
  CAAGACAGTGTCTCACTGATGAACTGGGCTTGCACGTTCAGCAGAACCGGGCAATACTGGACCATCAACA
  GTTTGACTACATAATAAGGATGATGAACTGTACTCTACAGGATTGTTCAAGTTTAGAAGAATATAACATC
  GCTGCAGCATTACTCCCCCTGACCAGTGCTTTCTATAGGAAACTTGCCCCTGGAGTCAGCCAGTTTGCCT
  ACACCTGTGTACAGGACCACCCCATTTGGACAAATCAGCAGTTTTGGGAGACAACTTTTTACAATGCAGT
  ACAGGAGCAGGTTCGCTCCCTGTATCTCTCAGCAAAGGACGACAATCATATCCCACATCTGAAGCAGAAG
  CTTCCCGATGGCCAGCATCAGGAGAAGACAGCCATGGACTTGGCAGCCGAGCAGCTTCGCCTATGGCCTA
  CCCTGAGCAAGTCAACTCAGCAGGAGCTGGTGCAGCACGAGGAGAGCACCGTCTTCAGCCAGGCCATTCA
  CTTTGCAAACCTCATGGTTAACCTGCTCGTTCCACTCGACACGAGTAAAAACAAGCTGCTTCGAGCATCC
  GCTCCAGGAGACTGGGAAAGTGGGAGCAACAGCATCGTCACAAACAGTATTGCAGGAAGTGTAGCTGAGA
  GCTATGATACAGAGAGTGGGTTTGAAGATTCGGAAAACAGTGATGTGGCCAACTCTGTTGTAAGGTTCAT
  TGCCCGATTTATTGACAAGGTTTGTACAGAGAGTGGAGTGACTCAGGACCACATCAGGAGCCTCCACTGC
  ATGATACCAGGAATTGTAGCTATGCACATTGAGACCCTAGAGGCAGTACATCGCGAGAGTAGAAGACTTC
  CACCTATCCAGAAGCCCAAGATTCTTCGACCAGCTCTACTGCCAGGAGAAGAAATTGTATGTGAAGGTCT
  TCGAGTCCTACTAGATCCTGATGGTCGCGAAGAAGCCACTGGAGGTCTCCTCGGAGGTCCTCAGCTCCTG
  CCTGCAGAAGGCGCCTTGTTCCTCACCACATACAGAATTCTCTTTAGAGGGACACCGCATGACCAGCTAG
  TTGGTGAACAAACAGTTGTGCGGAGCTTTCCCATTGCTTCAATCACCAAGGAGAAGAAGATAACCATGCA
  GAACCAGCTCCAGCAGAGCGTGCAGGAAGGGTTGCAGATCACATCAGCGTCTTTCCAGTTGATTAAAGTA
  GCATTTGATGAAGAAGTCAGTCCAGAAGTGGTCGACATCTTTAAGAAGCAGCTGATGAAGTTCCGTTACC
  CTCAGTCCATTTTCAGCACCTTTGCTTTTGCTGCTGGACAAACTACCCCACAAATCATTTTACCCAAGCA
  GAAAGAAAAGAACACCTCCTTCCGAACTTTCTCAAAAACAATTGTGAAAGGTGCCAAGAAGGCAGGAAAA
  ATGACAATTGGGAGGCAGTATTTATTAAAGAAGAGAACAGGAACAATTGTGGAAGAGAGAGTAAATCGTC
  CTGGGTGGAATGAAGAAGATGATATTTCTGTTTCAGATGATAGTGAACTTCCCACAAGTACAACCTTGAA
  GGCCTCAGAGAAGTCGACAATGGAACAGTTGGTGGAAAAAGCGTGTTTCAGAGATTATCAGCGGTTAGGT
  TTGGGAACCATAAGTGGCAACTCCTCTCGTTCAAAACCAGAGTATTTCCGAGTCACTGCCTCCAACAGGC
  TGTATTCGCTATGCCGGAGCTATCCTGGCCTTTTAGTCATACCTCAAGCTGTACAAGACAGTAGTTTACC
  AAGAGTAGCCCGCTGCTATCGACACAATCGCCTGCCCGTCGTATGTTGGAAGAACTCGAGAAGTGGTACT
  CTGCTCCTCCGATCTGGAGGATTCCATGGGAAGGGAGTTGTTGGTCTATTCAAGTCACAGAACTCCCCTC
  AAGCCGTTTCTACCTCCTCTTTAGAATCTTCCAGTAGCATAGAGCAAGAAAAGTACTTGCAAGCCTTATT
  GACTGCAGTCATTGTCCATCAGAAACTCAGAGGCAGCAGTACTCTCACTGTCAGACCAGCACTTGCTCTG
  TCCCCAGTGCATGGGTACAGAGACAAAAGTTTCACTCAGTCAAATCCCAAATCTTCAGCTAAGGAACCAG
  TTCATAATCAAGGCGTATGGGCAAGTCTTCGTTCCAGCACCCGTTTGATCAGCTCCCCGACATCCTTCAT
  TGATGTTGGTGCCAGGCTGGCAGGCAAGGACCACTCAGCCTCCTTCAGTAACAGTACCTACCTACAAAAC
  CAACTCTTGAAACGACAAGCCGCCCTTTATATATTTGGTGAAAAGTCACAGCTACGGAGCTCAAAGGTAG
  AATTCGCTTTCAACTGTGAGTTTGTTCCTGTTGAGTTCCATGAAATCCGACAAGTGAAAGCCAGTTTTAA
  GAAGCTGATGAGAGCTTGTATCCCAAGTACCATCCCTACAGACTCGGAAGTGACATTCCTGAAGGCCCTG
  GGAGATTCTGAGTGGTTCCCACAGCTTCACAGGATAATGCAGCTGGCTGTGGTTGTGTCAGAAGTACTTG
  AGAATGGGTCTTCAGTGTGGGTGTGTTTAGAAGAAGGCTGGGACATCACTACACAAGTGACATCCCTGGC
  ACAGCTACTCAGTGACCCCTTCTACAGGACAATCGCAGGCTTCCGGACGCTGGTGGAGAAGGAATGGCTC
  TCTTTTGGTCATAAGTTCAGCCAGCGCAGCAGCTTGGCCCTTAACTCTCAGGGCGGTGGCTTTGCTCCCA
  TCTTCTTGCAGTTCTTAGACTGTGTGCACCAGGTTCACAACCAGTATCCAACTGAGTTTGAATTCAATCT
  CTATTACCTAAAGTTCTTGGCTTTCCATTATGTGTCTAATCGCTTTAAAACATTTCTCCTGGATTCAGAC
  TATGAAAGACTAGAACATGGAACTTTATTTGATGATAAAGGTGACAAACATGCCAAGAAAGGAGTTTGTA
  TCTGGGAGTGTATTGACAAGATGCACACACGGAGTCCCATTTTCTTCAATTACTTATACTCACCGGTGGA
  AGTAGAGGCCCTGAAGCCTAATGTAAATGTCTCCAGCCTAAAGAAGTGGGATTACTACACAGAAGAGACC
  CTGTCTGCAGGTCCTTCATATGACTGGATGATGCTGACCCCCAAGCACTTTCCCTATGAGGAGTCAGATG
  TAGCCGGAGGAGCTGGGCCCCAGAGTCAGAGGAAGACAGTGTGGCCATGCTATGATGATGTGACCTGCAG
  CCAGCCAGATGCCCTCACGAGACTTTTCAGTGAAATTGAAAAATTGGAGCATAAATTGAATCAAACTCCT
  GAGAGATGGCACCAGCTATGGGAAAAAGTCACCACGGACCTCAAGGAAGAGCCAAGAACAGCCCATTCCC
  TAAGACACTCAGCGGGATCCCCAGGAATTGCATCTACCAACGTGCCTTCCTATCAGAAGAGGCCTGCGCT
  GCACCCGCTACACCGAGGCCTGGGCGAGGATCAGAGTACCACCACCGCCCCGTCCAATGGTGTAGAGCAC
  AGGGCGGCCACACTTTACAGCCAGTACACATCCAAGAACGACGAGAACAGATCCTTTGAAGGAACATTAT
  ACAAACGAGGAGCATTGCTGAAGGGTTGGAAACCCCGCTGGTTTGTTTTAGATGTCACGAAACACCAGCT
  GCGATACTATGATTCGGGGGAAGACACGAGTTGTAAAGGCCACATTGATCTGGCTGAAGTAGAAATGGTT
  ATCCCTGCCGGCCCCAGCATGGGAGCCCCAAAGTATACAAGTGACAAGGCTTTCTTTGATCTCAAGACCA
  GCAAGCGAGTGTATAACTTCTGTGCCCAGGATGGGCAGAGCGCCCAGCAATGGATGGACAGGATCCAGAG
  CTGCATCTCTGATGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177324
• Insert Size: 5619 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177324.2, NP_796298.2
• RefSeq Size: 7163 bp
• RefSeq ORF: 5619 bp
• Locus ID: 319934
• UniProt ID: E9PXF8
• Cytogenetics: 7 E3
• Gene Summary: Guanine nucleotide exchange factor (GEF) which activates RAB21 and possibly RAB28 (By similarity). Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (By similarity). In response to starvation-induced autophagy, activates RAB21 which in turn binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion (By similarity). Acts as an adapter for the phosphatase MTMR2 (PubMed:16399794). Increases MTMR2 catalytic activity towards phosphatidylinositol 3,5-bisphosphate and to a lesser extent towards phosphatidylinositol 3-phosphate (PubMed:16399794).[UniProtKB/Swiss-Prot Function]