Sbf1 (NM_001081030) Mouse Untagged Clone

SKU MC224992

Sbf1 (untagged) - Mouse SET binding factor 1 (Sbf1), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Sbf1
• Synonyms: 2610510A08Rik; B230113C15Rik; mKIAA3020; Mtmr5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224992 representing NM_001081030; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGCGGCTCGCGGACTACTTCGTGCTGGTGGCGTTCGGGCCGCACCCGCGCGGGAGTGGGGAAGGCC; AGGGCCAGATCCTGCAGCGCTTCCCGGAGAAGGACTGGGAGGACAACCCTTTCCCCCAGGGCATTGAGCT; GTTTTGCCAGCCCAGTGGGTGGCAGCTATGTCCCGAGAGGAATCCACCAACTTTCTTTGTGGCTGTCCTT; ACTGACATCAACTCTGAGAGGCACTACTGCGCCTGCTTGACCTTCTGGGAGCCAGTGGAGTCCACACAGG; AAGTAGTGTGCACTGACAATGCCACGGAGAAGGAGGAAGAGGCAGATGGAGGAGGCCAAGCGCGGCTGTC; ATCCACAGCCCCAGCCCAGCCTGGCCAGCTTTTTGCGCCAAAGACTCTGGTTCTGGTGTCTCGACTGGAC; CATGCCGAAGTGTTCAGGAATAGCCTTGGTCTCATCTATGCTATCCACGTGGAAGGCCTGAATGTGAGCC; TCGAGAATGTGATCGGGAACCTGCTCACATGTACCGTCCCATTGGCTGGCGGGTCTCAGAGAACCATCTC; TTTGGGGGCTGGTGACCGGCAGGTCATTCAGACTCCACTGGTAGACTCACTGCCTGTCAGCCGCTGTAGT; GTAGCCCTGCTCTTCCGCCAGCTGGGTATCACCAATGTGCTGTCTTTGTTCTGTGCTGCCCTTACTGAGC; ACAAAGTCCTCTTCTTGTCCAGGAGCTACCAACGTCTAGCAGATGCTTGCAGGGGTCTCTTGGCATTGCT; GTTCCCTCTCAGATACAGCTTCACTTATGTGCCCATCTTGCCGGCACAGCTGCTGGAAGTCCTTAGCACA; CCTACGCCTTTCATCATTGGGGTCAATGCAGCCTTCCAGGCAGAGACTCAGGAGCTGCTGGATGTGATTG; TTGCTGATCTTGACGGAGGGACAGTGACTGTCCCTGAGTGTGTGCACATTCCACCCCTGCCAGAGCCACT; ACAAAGCCAGACCCACAATGTTCTGAGCATGGTCCTGGATCCAGAACTGGAGTTGGCTGACCTTGCCTTC; CCACCCCCTACAACATCTGCTTCCTCCCTGAAAATGCAGGACAAGGAGCTCCGTGCTGTCTTTCTGCGGC; TCTTTGCTCAGCTCCTGCAAGGCTACCGCTGGTGCCTGCACATCGTGCGCATCCACCCAGAGCCCGTCAT; CCGCTTCCATAAGGCAGCATTCTTAGGCCAGCGTGGACTGGTGGAGGATGATTTCCTGATGAAAGTATTG; GAGGGCATGGCCTTCGCGGGCTTTGTATCTGAGCGTGGGGTTCCCTACCGTGCCACAGACCTGTTTGATG; AGCTGGTGGCTCATGAGGTAGCACGGATGCGAGCAGATGAGAGCCATCCCCACCGTGTCCTGCGTCATGT; CCAGGAACTGGCAGAGCAACTTTATAAGAATGAAAACCCGTACCCAGCTGTGGCGATGCACAAAGTGCAG; AGGCCAGGAGAGGCCAGTCACCTGCGGCGGACCCACCGGCCATTCCCCCGGCTAGATGAGGGCACAATTC; AGTGGATTGTGGACCAGGCTGCGGCCAAGATGCAGGGCGCACCTCCAGCTGTCAAGGCTGAGCGGAGGAG; CACTGTGCCTTCAGGGCCCCCCATGACGGCCATACTGGAGCGCTGTAGTGGGCCTCATATCAACAGTGCA; CGTCGCTTGGAGGTAGTACGGAATTGCATCTCCTATGTGTTTGAAGGGAAAATGCTTGAGGCAAAGAAGT; TGCTTCCGGCTGTACTCAGGGCCCTGAAGGGGCGAGCTGCCCGTCGCTGCCTTGCCCATGAGCTTCACCT; GCACGTACAGCAGAACCGGGCAGTTCTGGATCATCAGCAGTTTGACTTTGTCGTCCGCATGATGAATTGC; TGCCTGCAGGATTGCACTTCCCTGGATGAGCATGGCATTGCATCTGCACTGCTGCCTTTGGTCACAGCCT; TCTGCAGGAAGCTGAGCCCAGGGGTGACACAGTTTGCATACAGCTGTGTTCAGGAGCACGTAGTGTGGAG; CACACCTCAGTTCTGGGAGGCCATGTTCTATGGGGATGTGCAGACCCATATCCGAGCCCTCTACCTGGAG; CCCTCTGATGGCGTGAGCCCCACCCAGGAGACTGGGGAGGCACAGTCTCAGGATGATGAACGATCTGCCC; TGGATGTGGCTTCAGAGCAGAGGCGCCTGTGGCCAACCCTGAGCCGTGAGAAGCAGCAGGAGCTGGTACA; GAAGGAGGAAAGCACTGTGTTCAGCCAGGCCATCCACTATGCCAACCGCATGAGCTACCTTCTGCTGCCT; CTGGACAGCAGCAAGAGCCGGCTGCTGCGGGAGCGGGCAGGGTTGGGAGACCTGGAGAGTGCCAGCAACA; GCCTGGTCACCAACAGCATGGCAGGCAGTGTGGCTGAGAGCTATGACACAGAGAGTGGCTTTGAAGATGC; AGAGACGTGCGATGTGGCTGGGGCTGTAGTCCGCTTCATCAACCGCTTTGTGGACAAAGTCTGCACAGAG; AGTGGGGTCACCAGCGACCACCTCAAAGGACTGCATGTCATGGTGCCAGACATTGTCCAGATGCACATTG; AGACCCTGGAGGCCGTACACCGTGAGAGCAAGAGGCTGCCCCCGATACAGAAGCCCAAGCTGCTGAGGCC; ACGCCTGTTGCCTGGTGAGGAGTGTGTCCTCGATGGCCTTCGAGTGTATCTGCTGCCAGACGGGCGTGAG; GAGGGTGTAGGAGGCAGTGGAGGGGGCCCTGCTCTACTCCCAGCTGAGGGTGCTGTCTTCCTTACCACAT; ACCGCGTCATCTTCACGGGGATGCCTACTGACCCCCTGGTGGGGGAGCAGGTGGTAGTCCGCTCCTTCCC; CGTGGCTGCCTTGACCAAGGAGAAGCGCATTAGTGTGCAGACCCCTGTGGACCAGCTTCTGCAGGATGGG; CTGCAGCTTCGTTCCTGCACATTCCAGCTGCTGAAAATGGCCTTTGATGAGGAGGTGGGATCTGACAGTG; CTGAGCTCTTCCGAAAGCAGCTACACAAGCTTCGGTACCCACCAGACATCAGGGCCACCTTTGCATTCAC; ACTTGGCTCAGCTCACACACCTGGCAGGCCGCCACGGGTTACCAAGGACAAGGGTCCTTCATTCAGAACC; CTATCCCGGAACCTGATGAAGAATGCTAAAAAGACCATTGGGCGGCAGTATGTTACTCGTAAGAAGTATA; ACCCCCCTGGCTGGGAGCATCGGGGCCAGCCACCCCCTGAGGACCAGGAGGACGAGATCTCAGTGTCAGA; GGAGCTGGAGCCTAGCACACTGACCCCGTCCTCAGCCCTGAAGCCCTCTGACCGCATGACCATGAGCAGC; CTGGTGGAACGGGCATGTTGCCGTGACTACCAGCGCCTTGGACTAGGTACCCTGAGCAGCAGCCTGAGCC; GGGCCAAGTCTGAGCCCTTTCGCATCTCTCCTGTTAATCGCATGTATGCCATATGTCGCAGCTATCCAGG; ATTGCTGATTGTTCCCCAGAGCATCCAGGACAACGCCCTGCAGCGTGTATCCCGCTGCTACCGCCAGAAC; CGCTTCCCTGTGGTCTGCTGGCGCAGTGGGCGCTCCAAGGCTGTCTTGCTACGCTCTGGGGGCCTGCATG; GCAAAGGTGTTGTTGGTCTCTTCAAGGCCCAGAATACACCTTCTCCAGGCCAGGCCCAGGCCGACTCCAG; CAGCCTGGAGCAAGAGAAGTACCTGCAGGCTGTGGTCAGCTCCATGCCACGTTATGCTGACTCATCAGGA; CGAAACACACTGAGTAGCTTCTCCTCAGCCCACATGGGTGGCCATGGGAAATGGAGCAGTGTCCGAGCCA; GTGGTCGAAGCAGTGGTCTTGGTTCTGATGTGGGCTCTCGGCTAGCTGGCAGAGATCTCCTCAGTACTCC; CCACACCAACGGAGCTCCACCTGATTCTGGTTTTCTACGGCCACAGCGTGCAGCCCTCTACATCATTGGT; GACAAAGCTCAGCTCAAGGGTGTGCGCCCAGATCCCTTGCAACAGTGGGAGCTGGTACCTATTGAAGTAT; TTGAGGCAAGGCAGGTGAAAGCCAGCTTCAAGAAACTATTGAAAGCCTGTGTGCCTGGTTGCCCTGCCAC; TGAGCCCAGCCCAGCCTCTTTTCTGCGCTCACTGGAGGACTCAGAATGGCTGATCCAGATCCACAAGCTG; CTGCAGATATCGGTGCTGGTAGTGGAGCTGCTCGACTCTGGCTCCTCTGTCCTGGTGAGCCTGGAGGACG; GCTGGGACATCACCACTCAGGTGGTATCCTTGGTGCAGCTGCTCTCAGACCCCTTCTACCGCACTCTGGA; AGGCTTCCGACTGCTAGTGGAAAAAGAGTGGCTGTCCTTTGGCCATCGTTTCAGCCACCGCGGGGCCCAC; ACCCTAGCTGGGCAGAGCAGTGGCTTCACACCCGTCTTCCTGCAGTTCCTGGACTGTGTACACCAGGTCC; ATTTGCAGTTCCCCATGGAGTTTGAGTTCAGTCAGTTCTACCTCAAGTTCCTTGGTTACCACCACACATC; CCGCCGTTTCCGGACCTTCTTGCTGGACTCTGATTATGAGCGTATTGAGCTGGGGCTGCTGTATGAGGAG; AAGGGTGAACGCAGGGGTCAGCTGGCCTGCAAGTCAGTGTGGGAGTATGTAGACCGGCTAAGCAAGAGGA; CCCCCATGTTCTACAACTACACATATGCACCCGAGGACACAGAGGTGCTGCGACCCTACAGCAATGTGTC; CAATCTGAAGGTGTGGGATTTCTACACTGAGGAGACGCTGGCTGAGGGGCCCCCTTATGACTGGGAGCTG; GCCCAGGGACCCCCTGAGCCTCCAGAAGAGGAACGTCCTGATGGAGGTGCTCCCCAGAGCAGGCGCCGTG; TGGTATGGCCATGCTATGACAGCCGTCCTCGAGTCCAGCCTGATGCCATCTCACGTCTGCTGGAGGAGCT; GCAGCGCTTGGAGACAGAGTTAGGTCGGCCTTCTGAACGCTGGAAAGACACCTGGGACCGGGTGAAGGCT; GCTCAGCGCCTTGAAAGCCGGCAAGATGGACGTGGTACCCCCAGCTCACTTCTGGTGTCGGCTGTGCCCC; ACCACCGCCGCTCGCTAGGCGTCTATCTACAGGAGGGGCCTGTGGGCTCTACTCTTAGCCTCAGCCTGGA; CAGTGACCAGAGCAGTGGCTCAACCACATCTAGCTCTCGGCAGGCCGCCCGGCGGAGCACCAGCACCCTG; TACAGCCAGTTCCAGACAGCTGAGAGTGAGAACAGGTCCTATGAGGGCATCCTATACAAGAAGGGGGCCT; TCATGAAGCCCTGGAAAGCCCGTTGGTTTGTCCTAGACAAGACCAAGCACCAGCTGCGATACTATGACCA; CCGAATGGACACAGAATGCAAGGGTGTCATTGACCTGGCAGAGGTGGAAGCTGTGGCACCTGGCACACCC; ACTATAGGTGCCCCTAAGACTGTGGATGAGAAGGCCTTCTTTGATGTGAAGACAACACGTCGCGTTTACA; ACTTCTGTGCCCAGGATGTGCCCTCAGCCCAGCAGTGGGTGGACCGGATCCAGAGCTGCCTGTCGGATGC; CTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081030
• Insert Size: 5604 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001081030.2, NP_001074499.2
• RefSeq Size: 6190 bp
• RefSeq ORF: 5604 bp
• Locus ID: 77980
• UniProt ID: Q6ZPE2
• Cytogenetics: 15 E3
• Summary: Acts as an adapter for the phosphatase MTMR2 to regulate MTMR2 catalytic activity and subcellular location. May function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to represent an alternate splice pattern that is more supported by the available transcript data.