Pcdh15 (NM_001142737) Mouse Untagged Clone

CAT#: MC224987

Pcdh15 (untagged) - Mouse protocadherin 15 (Pcdh15), transcript variant D, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcdh15
• Synonyms: av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224987 representing NM_001142737
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA
  GCTGGGGCCAGTATGACGATGATTGCAAACTAGCTAGGGGAGGACCACCAGCTACTATCGTGGCCATTGA
  TGAAGAGAGTCGAAACGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACTGCCGGAGGACCAGAC
  CCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACCCCGTTAAACAGATGC
  TTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACACTCCATTGTGGTGCA
  AGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATCGTGGTGCGAGATCGG
  AATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGCTCACTCCAGTTGGCA
  CCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGGCCCTAATGGACAGAT
  AGAATACGTGATTCAGTACAACCCAGAAGATCCGACATCCAACGACACCTTTGAAATTCCACTCATGCTG
  ACTGGCAACGTGGTACTGAGGAAAAGACTCAACTATGAGGATAAGACTCGCTACTATGTCATCATCCAAG
  CAAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACCACCACCACCCTCACAGTAGATGTTCTAGA
  TGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTGTGCCAAACACACGTGACTGTCGTCCACTC
  ACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTTTGGTGACACCACCTA
  TCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCATCCTCTACTCCATCCT
  TGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGCATCCCAGGACTGCAGAACTCACTCTCCTG
  GAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGTTATTAAGGCTGAGCAGGACAATGGCCACC
  CACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGACGAAAACAATCAGAGTCCATACTTCACAAT
  GCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGGGAGCCACCATTTCTGAGAGCCTAAACTTA
  ACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACACAAAAGATCCAGAGCTCCACCTCT
  TCCTGAATGACTACACCTCGGTCTTCACTGTGACACCCACTGGTATCACCCGCTACCTCACCCTGCTTCA
  ACCTGTGGACAGGGAGGAACAGCAAACCTACACCTTTCTGATAACAGCGTTTGATGGCGTGCAAGAAAGT
  GAGCCAGTCGTGGTCAATATCCGAGTGATGGATGCAAATGATAACACGCCCACCTTCCCTGAAATCTCCT
  ATGATGTCTATGTTTACACAGACATGAGTCCTGGGGACAGCGTCATTCAGCTGACAGCGGTAGATGCTGA
  TGAAGGCTCTAATGGGGAGATCTCCTATGAAATACTGGTGGGGGGCAAGGGAGACTTCGTGATCAACAAG
  ACCACAGGGCTGGTGAGCATTGCACCAGGCGTGGAGCTGATCGTGGGACAGACGTATGCGCTCACAGTGC
  AGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAGCACAGGGATTCTCAGCCTAGGGAAGGCTCTAGACCG
  CGAGAGCACAGACCGCTACATCCTCATCGTCACAGCCTCAGATGGCAGACCGGATGGAACCTCAACTGCC
  ACTGTGAACATAGTGGTGACGGACGTCAATGACAACGCTCCCGTGTTCGATCCCTATCTGCCCAGGAACC
  TCTCTGTGGTGGAGGAAGAAGCCAATGCCTTTGTGGGTCAAGTCCGGGCAACAGACCCAGATGCTGGGAT
  AAACGGCCAAGTTCACTACAGCCTGGGGAACTTCAACAACCTCTTCCGCATCACATCCAACGGGAGCATT
  TACACAGCCGTGAAGCTGAACAGGGAAGCCAGGGACCACTATGAACTGGTTGTCGTGGCAACAGATGGAG
  CAGTCCACCCTCGACATTCAACTCTGACACTGTACATCAAGGTGTTGGACATTGATGATAACAGTCCTGT
  TTTTACCAATTCAACGTACACAGTTGTCGTTGAAGAGAATCTGCCAGCCGGGACCTCCTTTCTTCAAATA
  GAGGCCAAGGATGTTGACCTTGGAGCCAATGTGTCATATCGGATCAGAAGCCCAGAAGTGAAACACCTTT
  TTGCACTGCATCCATTCACTGGAGAATTGTCTCTTCTGAGGAGTTTGGATTATGAGGCCTTTCCGGACCA
  GGAGGCAAGCATCACATTCTTGGTGGAGGCCTTTGACATTTATGGGACTATGCCACCTGGTATAGCAACA
  GTCACGGTAATTGTGAAGGACATGAATGACTACCCTCCAGTGTTTAGCAAACGCATCTACAAGGGGATGG
  TGGCTCCAGATGCAGTCAAGGGGACACCAATCACCACCGTTTATGCTGAAGATGCGGACCCACCTGGGAT
  GCCTGCAAGTAGGGTGAGGTATCGAGTGGACGACGTGCAGTTTCCATACCCAGCCAGTATTTTTGATGTA
  GAGGAAGATTCTGGAAGAGTAGTAACCCGCGTCAATCTTAATGAAGAGCCTACTACGATTTTCAAGCTGG
  TGGTTGTGGCTTTTGATGACGGCGAACCTGTGATGTCCAGCAGTGCCACGGTGAGAATTCTTGTCTTACA
  TCCTGGAGAGATCCCACGCTTCACCCAAGAGGAATACAGACCTCCTCCTGTAAGTGAGCTTGCGGCCAGA
  GGGACTGTAGTTGGTGTCATTTCTGCTGCTGCCATTAATCAGAGCATCGTGTACTCCATTGTGGCAGGAA
  ATGAGGAAGACAAGTTTGGAATCAACAATGTCACTGGGGTCATCTATGTGAATTCACCATTGGATTACGA
  GACAAGGACCAGCTATGTGCTCCGGGTACAAGCAGATTCTCTGGAAGTGGTCCTTGCCAATCTCCGAGTC
  CCTTCAAAAAGCAATACAGCTAAGGTGTACATTGAGATTCAGGATGAAAACGATCACCCCCCAGTGTTCC
  AGAAGAAATTCTACATTGGAGGTGTGTCTGAAGACGCAAGGATGTTCGCATCTGTGCTCAGAGTGAAGGC
  CACCGACAGGGACACGGGTAATTACAGTGCCATGGCCTACCGGCTCATCATACCGCCGATTAAAGAGGGC
  AAAGAGGGGTTTGTGGTGGAAACATACACAGGTCTCATCAAGACAGCCATGCTCTTCCACAATATGAGAA
  GATCCTACTTCAAGTTTCAAGTGATTGCAACTGACGACTACGGGAAGGGGTTGAGCGGGAAAGCAGACGT
  ACTGGTCTCCGTGGTCAATCAACTGGATATGCAGGTCATTGTCTCCAATGTGCCCCCTACACTAGTGGAA
  AAGAAGATAGAAGACCTTACAGAGATTTTGGATCGCTACGTTCAGGAGCAAATTCCTGGTGCCAAGGTTG
  TGGTGGAGTCCATAGGTGCCCGTCGCCATGGAGACGCCTACTCCCTAGAAGACTATAGCAAGTGCGACCT
  GACTGTCTATGCCATCGACCCGCAGACCAACAGAGCCATCGACAGAAATGAGCTTTTTAAGTTCCTGGAC
  GGCAAACTGCTCGATATCAATAAAGACTTCCAGCCGTATTACGGGGAAGGAGGGCGCATTCTGGAGATTC
  GGACACCTGAGGCAGTGACGAGCATCAAGAAGCGAGGAGAAAGCTTGGGGTACACAGAAGGGGCCTTGCT
  GGCCTTGGCCTTCATCATCATCCTCTGTTGCATCCCAGCCATCTTGGTCGTCTTAGTAAGCTACCGACAG
  TTTAAAGTACGCCAGGCTGAGTGCACGAAGACCGCAAGAATTCAGTCTGCTATGCCTGCAGCCAAGCCTG
  CAGCTCCTGTACCAGCTGCGCCTGCGCCGCCCCCGCCCCCGCCACCACCACCACCAGGAGCACATCTCTA
  TGAAGAACTGGGAGAGAGCGCAATGCATAATCTTTTCCTTCTCTACCATTTTGAACAAAGCAGGGGAAAT
  AACTCAGTCCCAGAAGACAGGAGCAGTCATCGCGATGGGATGGCCTTTTCCTCCAGTACCACTGAGTCTC
  ATGAGCCAGCTCATGTAGAGGGACCACTTAAGGAGAGCCAGCCTAACCCAGCAAGGACGTTCTCATTTGT
  TCCTGATGAGGATAACTTAAGTACCCATAATCCCCTTTACATGGAAAGTATAGGTCAAAGGTCAACAAAC
  TCAGACCTTCAGCCACGAACAGATTTTGAAGAGCTGTTGGCACCCAGAACACAAGTTAAGAGTCAGTCTC
  TGAGGGGCCCAAGAGAAAAGATCCAGAGGGTGTGGAATCAGTCTGTGAGCTTTCCTAGGCGGCTCATGTG
  GAAAGCCCCAAACAGGCCAGAGACCATAGACCTGGTGGAGTGGCAGATCACCAATCAGAGAGCTGAATGC
  GAAAGCGCCAGATGCCACCCAAGCCAGAGAGGTAGCAGCAACGTTCTGCTGGCAACTGAAGATGCCCACG
  AGTCAGAGAAAGAAGGGGGACACAGAGACACCCTAATCGTCCAGCAAACAGAGCAGCTGAAATCTCTGTC
  TTCTGGCTCTTCTTTTTCCTCCTCTTGGTCTCACTTTTCTTTCTCAACTCTGCCAACGATTTCCAGAGCG
  GTGGAACTCGGGTCGGAACCTAATGTGGTCACTTCTCCCGCTGACTGCACCTTGGAACTTTCTCCTCCTC
  TGAGACCCCGTATTTTAAACTCCTTAAGCTCTAAGAGAGAGACTCCCACATGTGCATCAGATACAGAACC
  AAAAAGGAACTCTTTTGAGATCGCTCCCCATCCACCTAGCATCTCTGCTCCCCTCCCACATCCGCCTCTT
  CCTAGACCTCCCATTGCCTTTACCACTTTTCCTCTTCCCCTTTCTCCCCCTAACCCTCCTCCCCCACAAC
  TTGTTACATTTTCTCTTCCCATTTCTACACCCCCTACTTCTTCTCTACCTCTTCCTCCTCCACTGTCACT
  TCCTCCTCCTCCTCGGCCACCAGCTCCCCGCCTCTTCCCACAGCCTCCTTCCACGTCCATTCCATCCACA
  GACAGCATCTCTGCACCAGCTGCTAAATGCACTGCCAGTGCCACACACGCCAGAGAAACCACGTCTACGA
  CACAGCCACCAGCATCCAACCCGCAGTGGGGGGCAGAACCCCACAGACATCCAAAAGGGATCCTCAGACA
  TGTGAAAAACTTGGCAGAGCTCGAGAAATCAGTGTCTAACATGTACAGTCACATAGAAAAAAACTGCCCA
  CCTGCAGATCCCTCAAAACTACACACGTTTTGCCCTGCAGAGAAAACAGGCATGAAAATCACACATGACC
  AGAGCCAGGAAACGTTGGTTAGAGTTGTTGAGGGAATTGACGTGCAACCTCACAGTCAATCAACATCTTT
  GTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142737
• Insert Size: 5604 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142737.1, NP_001136209.1
• RefSeq Size: 8836 bp
• RefSeq ORF: 5604 bp
• Locus ID: 11994
• UniProt ID: Q99PJ1
• Cytogenetics: 10 37.43 cM
• Gene Summary: Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (D) lacks an alternate in-frame exon in the 5' coding region and two alternate in-frame exons in the central coding region, compared to variant A. The resulting isoform (CD1-6), also known as protocadherin-15-CD1 isoform 6, lacks a 5-aa segment near the N-terminus and a 71-aa segment in the central protein, compared to isoform CD1-1.