Virma (NM_001081183) Mouse Untagged Clone

CAT#: MC224983

1110037F02Rik (untagged) - Mouse RIKEN cDNA 1110037F02 gene (1110037F02Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Virma
• Synonyms: 4930422M05Rik; Kiaa1429; mKIAA1429
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224983 representing NM_001081183
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGGACTCGTCTATGGAGCTGCTATTTTTAGATACTTTTAAACATCCGAGCGCTGAGCAAAGTT
  CTCATATAGATGTGGTTCGTTTTCCGTGTGTGGTTTATATCAATGAAGTCCGAGTTATACCCCCGGGAGT
  ACGAGCCCATAGTGGCTTACCTGACAACAGAGCATATGGGGAAACATCTCCTCACACATTTCAACTTGAC
  TTATTCTTCAATAATGTAAGCAAACCAAGTGCTCCAGTTTTTGATAGGTTGGGAAGCCTGGAATATGATG
  AGAATACGTCTATCATCTTTAGACCAAACTCGAAGGTAAATACTGATGGTCTGGTACTAAGAGGCTGGTA
  CAACTGTCTGACCCTGGCAATATATGGATCGGTAGATAGAGTGATAAGCCATGACAGAGACTCTCCACCA
  CCTCCACCTCCACCTCCACCTCCTCCCCAGCCACAGCCGACTTTGAAAAGGAATCTAAAGCATGCTGATG
  GTGAGAAGGAAGATCAGTTTAATGGAAGTCCTCCAAGACCCCAGCCACGGGGACCAAGAACTCCTCCTGG
  ACCTCCTCCACCTGATGACGATGAGGACGACCCTATGTCTTTGCCAGTGTCTGGTGACAAAGAAGAGGAT
  GTTCCCCATCGAGAAGATTACTTTGAGCCCATTTCTCCTGATAGGAATTCTGTCCCGCAGGAAGGTCAGT
  ATTCCGATGAAGGAGAGGTAGAAGAGGAACCGCAAGAAGAGGGAGAAGATGATGAAGATGATGTGGATGT
  AGAGGAAGAAGAGGATGAAGATGAGGATGACTGCCATACAGTAGATAGTATCCCTGATGATGAGGAAGAA
  GATGAAGAGGAAGAAGGTGAAGAGGATGAAGAAGGTGAAGGGGACGATGGCTACGAGCAGATCTCTAGTG
  ATGAAGATGGAATTGCTGACTTGGAACGTGAAACATTCAAGTATCCAAACTTCGATGTTGAGTATACTCC
  AGAAGACCTGGCATCTGTCCCTCCTATGACGTATGACCCGTATGACAGGGAGCTGGCACCACTCTTGTAT
  TTCAGCTGTCCATACAAAACTACTTTTGAAATTGAAATCAGTAGAATGAAGGATCAAGGTCCAGATAAAG
  AGAATTCAGGGGCAGTAGAAGCCTCGGTGAAGTTAACTGAACTGCTAGACCTGTATCAGGAAGACAGGGG
  TGCAAAGTGGGTGACGGCTCTAGAAGAAATTCCAAGTCTAATTATAAAAGGATTAAGCTATTTGCAATTG
  AAAAACACAGAACAAGACTCCCTTGGCCAGTTGGTAGACTGGACAATGCAAGCTTTAAATTTACAAGTAG
  CCTTTCGACAGCCCATAGCCTTAAATGTCCGACAGCTCAAAGCTGGGACCAAGCTAGTGACCTCATTAGC
  AGAATGTGGGGCTCCAGGAGTCACAGAATTGCTACAAGCAGGAGTGATCAATGTATTGTTTGATCTCTTA
  TTTGCTGATCATGTCTCATCTTCCCTTAAGTTAAATGCTTTTAAAGCTTTGGACAGTGTCATTAGCATGA
  CAGAAGGAATGGAGGCTTTTTTAAGAAGCACCCAAAATGAGAAAAGTGGCTATCAAAGACTTCTGGAACT
  CATACTTCTGGATCAGACTGTGAGGGTTGTCACTGCTGGCTCAGCTATTCTTCAAAAATGCCATTTCTAT
  GAAATCTTGTCAGAGATTAAAAGACTTGGTGACCACATAGCAGAGAAGACTTCAGCTGTTCCTAACCACA
  GTGAGCCTGACCAGGACACAGATGCTGTGCTGGAGAGAGCAAATCCCGATTATGAGAATGAAGTAGAAGC
  TTCTATGGACATGGATCTTTTGGAGTCCTCAATTATAAGTGAAGGTGAAATAGAAAAGCTTACTAATCTC
  TTAGAGGAGGTTTTTCATGTCATGGAAACTGCACCTCATACAATGACCCAACCTCCTGTCAAGTCTTTCC
  CCACAATAGCCCGGATTACCGGGCCTCCAGAGCGGGATGACCCGTACCCTGTCCTCTTTAGAGGGCCTGG
  GTTTGGTTTTCAGCACTCAATAGTGGCTCACAGCCATCAGCTACTCCAGTTCCAGAGGGTCCAGTGCCCT
  CTTCTGGCCTATGTGGGCACTGTACACACATGGTACATAGACACATGCAAGCAAAATACTCATGACATAA
  GGTATCTTCACAGCCATCACTTCCTGGAGTTGGTCACCTTGCTTCTGTCGATTCCAATAACAAGTGCCCA
  TCAGGGTGTACTGCAAGCCACGAAGGATGTTTTAAAGTTTCTTGCACAGTCACAAAAGGGCCTTCTCTTT
  TTTATGTCAGAATATGAAGCGACAAACTTGTTGATCCGAGCTCTGTGCCACCTTTATGATCAAGACGAAG
  AGGAAGGCCTTCAGTCTGATGGGGCTGATGATGCCTTTGCCTTGTGGCTGCAGGACTCAACTCAGACTCT
  GCAGTGCATCACAGAGCTGTTCAGCCATTTCCAGCGGTGTACAGCCAGTGAAGAAACTGACCATTCTGAT
  CTCCTGGGCACCCTCCACAATCTTTACTTGATTACTTTTAACCCTGTGGGAAGATCAGCTGTTGGCCATG
  TTTTCAGCCTTGACAAAAACCTTCAAAGTCTCATTACTCTAATGGAGTACTATTCCAAAGAAGCCCTAGG
  CGATTCCAAGTCTAAGAAGTCAGTAGCTTATAATTACGCATGCGTACTCACGCTGGTGGTGGCTCAGTCT
  TCCAGCGGTGTCCAGATGTTAGAACAGCACGCAGCATCGCTCTTGAAGCTCTGCAAAGCAGATGAAAATA
  ATGCGAAATTGCAAGAACTTGGCAAATGGCTTGAACCTCTGAAAAACCTCAGATTTGAAATTAACTGCAT
  CCCAAATCTAATTGAATATGTTAAACAGAATATTGATAACTTGATGACTGCAGAAGGAGTTGGCCTTACC
  ACTGCCTTACGTGTTCTCTGTAATGTGGCATGTCCACCACCTCCCGTAGAAGGTCAGCAGAAAGACCTGA
  AATGGAACCTTGCTGTTATTCAGCTTTTTTCTGCTGAAGGAATGGACACCTTTATTCGGGTTCTGCAAAA
  ACTGAACAGTATTTTGACGCAGCCTTGGAGGCTCCATGTGAACATGGGGACTACCCTGCACAGAGTCACT
  ACCATTTCAATGGCTCGCTGCACGCTCACTCTGCTTAAAACTATGCTGACAGAGCTCCTGCGAGGCGGAT
  CCTTTGAGTTTAAGGACATGCGTGTTCCTTCAGCACTTGTTACTTTACACATGCTCCTGTGCTCTATCCC
  TCTCTCAGGTCGTTTGGATAGTGATGAGCAGAAAATTCAGAATGATATCATAGATATCTTACTGACTTTT
  ACACAAGGAGTCAATGAGAAGCTCACGATCTCGGAAGAGACTCTGGCCAATAATACTTGGTCTTTAATGT
  TAAAGGAAGTTCTCTCTTCCATCTTGAAGGTTCCGGAAGGCTTCTTCTCAGGACTCATACTCCTTTCAGA
  GCTGCTGCCTCTTCCCTTGCCCATGCAGACCACGCAGGTTATTGAGCCACATGATATATCAGTGGCACTC
  AACACCCGAAAATTGTGGAGCATGCACCTTCATGTTCAAGCAAAGTTGCTCCAAGAAATAGTTCGTTCTT
  TCTCTGGCACAACCTGCCAGCCCATTCAACATATGTTACGGCGTATTTGTGTTCAATTGTGTGACCTTGC
  CTCTCCAACTGCTCTTCTGATCATGAGAACTGTGTTGGATTTGATTGTAGAAGACTTGCAAAGCACTTCA
  GAAGATAAAGAAAAACAGTATACTAGCCAAACCACCAGGTTGCTTGCTCTTCTCGATGCCCTGGCTTCAC
  ACAAAGCTTGTAAATTAGCTATTTTGCATCTAATTAATGGAACTATTAAAGGCGATGAAAGATATGCAGA
  GATCTTCCAGGATCTTTTAGCTTTGGTGCGCTCTCCTGGGGACAGTGTTACTCGCCAGCAGTGCGTGGAG
  TATGTCACATCCATTTTGCAGTCTCTCTGTGACCAGGACATTGCACTCATTTTGCCAAGCCCTTCTGAAG
  GTCCTGCCTCTGAGCTTGAACAGCTCTCTAACTCCCTACCAAGTAAAGAGCTGATGACTGCAATCTGCGA
  CTGTCTGCTGGCAACTCTAGCCAACTCTGAAAGCAGTTACAACTGTCTGCTGACATGTGTCCGTACAATG
  ATGTTTCTTGCAGAGCACGATTATGGATTGTTTCACCTGAAAAGCTCTTTAAGGAAGAACAGTAGTGCTC
  TGCATAGTTTGCTGAAGCGAGTGGTCAGCACATTCAGTAAGGACACCGGTGAGCTTGCATCGGCGTCTCT
  GGACTTCATGAGACAGATCCTCAATGCTGATGCTATGGGCTGTTGTGGAGACGATAGTGGTCTCATGGAG
  GTAGAGGGCGCTCACCCACCCCGGACGATGAGTCTTAACGCTGCAGAGTTAAAACAGCTCCTACAGAGCA
  AAGAAGAGAGTCCAGAGAGTCTGTTCCTTGAACTAGAGAAGCTTGTTTTGGAACATTCTAAAGATGATGA
  CAGTCTGGAGTCTTTGCTGGATAATGTAATTGGACTGAAGCAGATGCTGGAGTCATCTGGTGAGCCTTTG
  CCTCTCAGTGACCAAGATGTAGAGCCAGTACTGTCAGCTCCAGAGTCTCTCCAGAATTTATTTAACAACA
  GGACTGCATATGTGTTGGCTGATGTCATGGATGATCAGTTGAAATCTATGTGGTTTACTCCATTTCAGGC
  TGAAGAGATCGATACAGACTTGGATTTGGTAAAGGTTGACTTAATTGAGCTTTCAGAAAAGTGCTGCAGT
  GATTTTGATTTGCATTCAGAACTAGAGCGCTCGTTTCTGTCAGAACCATCATCTCCAGGAAGATCCAAGA
  CTACTAAGGGATTTAAACTCGGGAAGCATAAGCATGAGACATTCATAACTTCAAGTGGAAAATCTGAATA
  CATTGAACCTGCCAAACGAGCTCATGTTGTGCCGCCACCTAGAGGCCGGGGCCGGGGAGGATTTGGACAG
  GGCATACGGCCCCATGATATTTTCCGTCAGAGAAAGCAGAACACGAGCAGGCCGCCATCTATGCACGTGG
  ATGACTTTGTTGCAGCTGAAAGTAAAGAAGTGGTCCCTCAAGATGGAATACCCCCACCAAAACGGCCACT
  CAAAGTCTCACAAAAGATTTCCTCTCGTGGTGGATTTTCAGGCAACCGAGGAGGACGGGGTGCTTTTCAT
  AGTCAGAACAGGTTTTTCACACCGCCTGCTTCGAAAGGAAACTACAGTCGTCGGGAAGGAACAAGAGGCT
  CGAGTTGGAGTGCTCAGAACACTCCTCGAGGAAATTATAATGAAAGCCGAGGGGGCCAGAGCAACTTTAA
  CAGGGGTCCTCTTCCACCATTACGGCCGCTTAGTTCTACGGGTTACCGCCCTAGTCCCCGGGATCGAGCT
  TCTAGAGGCCGCGGGGGCCTGGGACCGTCCTGGGCTAGTACAAATAGCGGCAGTGGAGGCTCGAGAGGAA
  AGTTTGTTAGTGGAGGCAGTGGTAGAGGCCGTCATGTACGGTCCTTTACTCGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081183
• Insert Size: 5586 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081183.1, NP_001074652.1
• RefSeq Size: 7115 bp
• RefSeq ORF: 5586 bp
• Locus ID: 66185
• Cytogenetics: 4 A1
• Gene Summary: Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs in the 3' UTR near the stop codon: recruits the catalytic core components METTL3 and METTL14, thereby guiding m6A methylation at specific sites. Required for mRNA polyadenylation via its role in selective m6A methylation: m6A methylation of mRNAs in the 3' UTR near the stop codon correlating with alternative polyadenylation (APA).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.