Col27a1 (NM_025685) Mouse Untagged Clone

CAT#: MC224979

Col27a1 (untagged) - Mouse collagen, type XXVII, alpha 1 (Col27a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Col27a1
• Synonyms: 5730512J02Rik; AI449266
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224979 representing NM_025685
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACGGGATTCGCGCGGGGGGCCCGAGGCACAGCGGCGTCAGGACCCGGGGGGGGGTTTCTCTTCG
  CCTGGATCTTGGTCTCATTTACCTGTCACCTGGCCTCCACCCAAGGAGCTCCTGAAGATGTGGATGTCCT
  CCAGCGGCTGGGCCTCAGCTGGACGAAGGCCGGGGGTGGCCGGAGTCCTACACCCCCTGGTGTCATTCCT
  TTCCCATCTGGCTTCATCTTCACACAGCGGGCCAAGCTGCAGGCCCCCACAGCCAATGTTCTTCCCACCA
  CCCTGGGGCGGGAGCTGGCATTGGTGTTGAGCCTCTGTTCACATCGTGTGAACCATGCCTTCCTCTTTGC
  CATCCGCAGCAGGAAACACAAGCTGCAACTGGGTCTGCAGTTCCTCCCCGGCAGGACGATCATCCACCTG
  GGGCCTCGGCAATCAGTGGCCTTCGACTTGGATGTGCATGATGGGCGCTGGCACCACCTGGCCCTAGAGT
  TGCGTGGCCGCACAGTCACAATGGTGACAGCCTGTGGGCAGCACAGGGTACCTGTCCCACTGCCTTCCCG
  TAGGGACTCCATGCTTGACCCCCAGGGCTCCTTTCTCTTGGGGAAGGTGAACCCCCGTGCTGTCCAGTTT
  GAAGGTGCACTCTGTCAGTTCAGCATCCACCCTGTGGCGCAGGTCGCTCACAATTACTGTGCCCACCTGA
  GAGAGCGGTGCCGACAGGTGGACACATATAGTCCCCAGGTGGGAACCCTCTTTCCTTGGGACTCTGGCCC
  AGCCTTTGCTTTGCATCCTGAACCAGCCTTGCTTGGCTTGGGGAATCTGACCAGAACTCCAGCAACCCTG
  GGGGCCAGGCCTGTAAGCAGGGCACTTGCAGTGACTCTGGCTCCTGCCATGCCCACCAAGCCCCTGAGGA
  CTGTCCACCCAGATGTCTCTGAACACAGCTCTTCCCAGACCCCACTGTCACCTGCCAAACAGTCGGCCAG
  GAAAACACCTTCCCCCTCCTCGTCAGCTTCCCTGGCTAATTCCACCAGGGTTTATCGTCCAGCTGCTGCC
  CAGCCAAGACAGATCACAACTACTAGCCCCACCAAACGTTCCCCCACCAAGCCTTCTGTTTCTCCCCTTT
  CAGTTACCCCCATGAAAAGTCCCCATGCAACCCAGAAAACAGGTGTCCCTTCATTCACAAAGCCTGTTCC
  ACCCACTCAGAAGCCAGCGCCCTTCACCTCCTACCTAGCACCTTCCAAAGCCTCTAGTCCCACTGTGAGG
  CCTGTTCAGAAGACTTTCATGACACCCCGACCTCCAGTCCCCAGCCCCCAGCCCCTGCGCCCTACTACTG
  GCTTATCTAAGAAGTTCACTAACCCCACTGTTGCAAAATCTAAGTCCAAGACGACCAGTTGGGCCAGCAA
  GCCGGTCTTGGCCCGCTCCAGTGTCCCTAAAACGCTTCAGCAAACTGTTTTGTCCCAGTCTCCTGTTTCC
  TATCTGGGCTCTCAGACACTGGCTCCAGCCCTCCCTCCTTTGGGTGTTGGAAATCCTAGAACGATGCCTC
  CCACACGTGACTCTGCTTTAACTCCTGCTGGAAGCAAGAAATTCACAGGACGGGAAACCTCCAAGAAAAC
  CAGACAGAAGAGCAGCCCCCGGAAGCCCGAACCTCTCAGCCCTGGGAAGTCAGCCAGGGATGCCTCACCA
  AGAGACCTGACAACCAAGCCTAGCCGGCCGTCCACCCCAGCCCTGGTCCTTGCCCCAGCTTACCTCCTGT
  CCTCCAGCCCCCAGCCCACCAGCAGCAGCTTCCCTTTCTTCCACCTGCTGGGCCCTACACCTTTCCCGAT
  GCTGATGGGGCCTCCAGGCTCCAAGGGAGACTGTGGCTTGCCGGGTCCCCCTGGGCTTCCAGGATTACCT
  GGATCTCCGGGCGCACGAGGTCCTCGGGGTCCTCCTGGGCCGTACGGGAACCCAGGCCCACCTGGCCCTC
  CAGGAGCCAAAGGACAGAAAGGGGACCCAGGACTCTCACCAGGACAGGCTCACGATGGAGCAAAGGGCAA
  CATGGGCTTGCCTGGGCTCTCCGGAAATCCAGGACCCTTGGGAAGGAAGGGACACAAGGGCCATCCTGGA
  GCAGCAGGGCACCCTGGAGAACAGGGGCAGCCAGGACCTGAGGGCAGTCCAGGGGCCAAAGGTTACCCTG
  GCAGGCAGGGGTTCCCTGGACCTGTAGGAGACCCTGGCCCCAAAGGCAGCAGGGGTTACATTGGTCTTCC
  GGGGCTCTTTGGCCTGCCAGGGTCGGATGGAGAGCGGGGTCTCCCTGGCGTTCCTGGCAAGAGGGGCGAG
  ATGGGTAGGCCGGGCTTCCCTGGAGACTTTGGGGAAAGAGGCCCCCCTGGACTTGATGGCAACCCTGGAG
  AAATTGGCCTGCCGGGGCCACCAGGAGTGCTCGGGCTCATTGGTGACACAGGAGCACTGGGCCCAGTTGG
  CTACCCAGGACCGAAAGGCATGAAGGGACTGATGGGCGGCGTGGGGGAGCCCGGACTGAAAGGTGATAAG
  GGTGAACAAGGGGTGCCAGGTGTGTCGGGAGATCCTGGCTTCCAAGGAGACAAGGGGAGTCATGGCTTGC
  CAGGGCTCCCGGGTGGCCGGGGGAAGCCAGGGCCTCTGGGAAAAGCTGGAGACAAAGGATCACTTGGGTT
  TCCTGGACCTCCTGGACCTGAGGGTTTCCCAGGAGACATTGGCCCTCCAGGGGACAATGGCCCTGAAGGC
  ATGAAGGGTAAGCCTGGAGCCCGAGGCCTGCCTGGACCTCCTGGGCAGCTGGGGCCTGAGGGAGATGAAG
  GACCCATGGGGCCACCAGGAGTCCCAGGCTTGGAGGGTCAGCCTGGAAGGAAAGGGTTTCCTGGGAGGCC
  TGGCCTGGATGGCTCGAAGGGGGAACCAGGGGATCCTGGACGGCCAGGACCTGTGGGTGAACAGGGGCTT
  ATGGGATTCATTGGTCTTGTCGGAGAGCCTGGAATTGTTGGGGAGAAGGGAGACCGTGGGGTGATGGGAC
  CCCCAGGTGCACCTGGACCCAAGGGGTCGATGGGCCATCCTGGAACACCCGGTGGTATTGGGAACCCTGG
  AGAGCCTGGACCCTGGGGCCCTCCAGGATCTCGAGGCCTGCCAGGCATGAGGGGAGCCAAGGGGCACCGG
  GGCCCTCGAGGACCTGATGGACCAGCTGGGGAGCAGGGATCCAAGGGCCTGAAGGGTCGTGTAGGACCTC
  GGGGCAGACCTGGCCAGCCAGGACAGCAGGGTGCAGCTGGTGAGCGAGGCCACTCAGGTGCAAAAGGCTT
  CCTTGGCATCCCTGGTCCCTCAGGCCCTCCAGGTGCCAAGGGCCTCCCAGGAGAACCGGGCTCTCAGGGA
  CCTCAGGGGCCAGTTGGTCCTCCAGGAGAAATGGGGCCCAAGGGACCGCCGGGTGCAGTGGGAGAGCCTG
  GCCTTCCTGGGGACTCCGGAATGAAGGGTGACCTCGGACCTCTGGGCCCCCCTGGGGAGCAAGGTCTTAT
  TGGGCAGCGGGGAGAGCCAGGCCTTGAGGGAGACCATGGCCCTGTGGGGCCAGATGGGCTGAAGGGCGAC
  AGGGGCGACCCAGGGCCCGATGGCGAGCATGGTGAAAAGGGCCAGGAAGGGCTGAAAGGAGAGGATGGGT
  CCCCTGGGCCCCCGGGCATCACTGGCGTCCCGGGTCGTGAGGGGAAGCCGGGCAAGCAGGGTGAGAAAGG
  CCAGAGGGGAGCTAAGGGTGCCAAGGGCCACCAAGGATATCTGGGAGAGATGGGCATCCCCGGAGAACCT
  GGGCCCCCTGGGACCCCAGGTCCTAAAGGGTCCCGGGGCACCCTAGGACCAACGGGTGCTCCAGGACGGA
  TGGGAGCCCAAGGAGAACCAGGATTGGCCGGTTATAATGGACACAAAGGCATCACAGGACCCCTCGGGCC
  TCCCGGGCCCAAAGGCGAGAAGGGGGACCAGGGCGAGGATGGCAAGACCGAGGGGCCCCCTGGGCCACCA
  GGAGATCGGGGTCCTGTGGGTGATCGAGGAGACCGTGGGGAGCCAGGCGACCCTGGATACCCTGGTCAAG
  AGGGAGTTCAAGGCCTCCGTGGAGAACCGGGCCAGCAGGGACAGCCTGGGCATCCAGGACCCCGGGGGCG
  CCCAGGACCCAAAGGATCAAAAGGCGAAGAGGGCCCAAAGGGAAAGCCAGGCAAGGCTGGGCCATCAGGC
  CGGAGGGGGACCCAGGGCCTTCAAGGACTGCCAGGCCCCCGAGGCGTGGTGGGGAGACAGGGCCCTGAGG
  GCACTGCTGGATCTGATGGGATTCCTGGCAGAGATGGTCGGCCAGGATATCAGGGAGACCAGGGAAATGA
  TGGGGACCCTGGCCCTGTGGGCCCTGCTGGGAGAAGAGGAAATCCAGGTGTGGCTGGCTTGCCTGGAGCA
  CAGGGGCCTCCGGGATTCAAGGGTGAAAGTGGGTTACCTGGGCAACTGGGTCCCCCTGGAAAACGAGGGA
  CAGAAGGTGGAACAGGGCTTCCTGGGAACCAGGGGGAGCCAGGATCCAAAGGCCAGCCGGGTGACTCTGG
  CGAGATGGGCTTCCCAGGAGTGGCTGGCCTCTTTGGACCCAAGGGCCCCCCTGGAGACATTGGCTTCAAA
  GGCATCCAAGGTCCTCGGGGGCCTCCTGGCTTGATGGGAAAGGAAGGTATCATTGGCCCCCCCGGAATGC
  TGGGACCTTCTGGACTCCCGGGTCCCAAAGGTGACAGAGGCAGCCGAGGAGACTTGGGACTGCAAGGCCC
  AAGGGGTCCTCCTGGTCCAAGGGGCCGGCCAGGTCCCCCGGGCCCTCCTTGGCATCCCATCCAGTTTCAG
  CAAGATGACCTTGGAGCAGCTTTCCAGACATGGATGGATGCTCAAGGAGCCGTCAGATCGGAGGGGTACA
  GCTATCCGGACCAGCTGGCGCTAGACCAGGGAGGGGAGATCTTCAAAACCTTACACTACCTCAGCAACCT
  CATCCAGAGCATTAAGACACCCTTGGGCACCAAGGAGAACCCAGCCCGGGTCTGCCGGGACCTCATGGAC
  TGTGAGCAGAGGATGGCGGATGGTACCTACTGGGTGGACCCCAACCTCGGCTGCTCCTCTGACACCATTG
  AAGTCTCCTGCAACTTTACACAGGGTGGGCAGACGTGCCTGAAGCCCATCACGGCCTCCAAGGCCGAGTT
  TGCTGTGAGTCGGGTCCAGATGAATTTCTTGCACCTGCTGAGCTCTGAGGGGACACAGCATATCACAATC
  CACTGTCTGAACATGACGGTGTGGCAGGAGGGACCGGGACGCTCCTCTGCCAGACAGGCTGTGCGCTTCC
  GTGCCTGGAACGGACAGGTCTTCGAAGCTGGGGGTCAGTTCAGGCCAGAGGTGTCTATGGATGGCTGCAA
  GGTCCATGATGGCCGCTGGCATCAGACACTGTTCACCTTCCGGACCCAGGACCCCCAGCAGCTGCCCATC
  GTCAGTGTGGACAATCTCCCGCCTGTCTCATCAGGGAAGCAGTACCGCCTGGAAGTTGGACCTGCGTGCT
  TCCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025685
• Insert Size: 5538 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025685.3, NP_079961.3
• RefSeq Size: 7635 bp
• RefSeq ORF: 5538 bp
• Locus ID: 373864
• UniProt ID: Q5QNQ9
• Cytogenetics: 4 B3
• Gene Summary: This gene encodes the alpha-1 subunit of type XXVII collagen, one of the low abundance fibril-forming collagens found in cartilage. The encoded protein forms a homotrimeric triple helical procollagen that undergoes proteolytic processing during fibril formation. Transgenic mice lacking a portion of the collagenous domain in the encoded protein exhibit skeletal abnormalities, chondrodysplasia and die at birth because of a lung defect. [provided by RefSeq, Dec 2015]