Arfgef1 (NM_001102430) Mouse Untagged Clone

CAT#: MC224976

Arfgef1 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arfgef1
• Synonyms: ARFGEP1; BIG1; D130059B05Rik; D730028O18Rik; P200
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224976 representing NM_001102430
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGAGGGCAAGAAGACGAAGAACATGTTCCTGACCCGGGCCCTGGAGAAGATTCTGGCCGACAAGG
  AAGTGAAGAAGGCGCATCACTCCCAGCTGCGCAAAGCGTGCGAGGTGGCCCTAGAGGAAATAAAAGTTGA
  AACTGAGAAACAAAGCCCTCCTCATGGAGAAGCAAAAGCTGGATCAGGCACCCTTCCACCAGTGAAATCA
  AAGACAAATTTTATTGAAGCAGACAAGTACTTTTTACCCTTTGAGTTGGCATGCCAGTCCAAATGTCCCC
  GCATAGTTAGTACATCTCTAGATTGTTTGCAGAAACTTATTGCTTATGGGCACTTGACTGGCAGGGCTCC
  AGATAGTACGACACCAGGCAAAAAATTAATTGATAGAATTATTGAAACAATATGTGGCTGCTTCCAAGGT
  CCTCAAACAGATGAAGGAGTTCAGTTGCAGATAATAAAGGCTTTACTTACCGCAGTGACATCACAACACA
  TAGAAATCCATGAAGGAACTGTGCTGCAAGCTGTGAGGACATGCTACAATATCTATTTAGCCAGCAAAAA
  TCTCATCAATCAGACAACAGCCAAAGCTACTCTTACTCAAATGCTGAACGTTATCTTTGCACGCATGGAA
  AACCAAGCATTACAGGAGGCCAAACAAATGGAAAGAGAAAGACATCGGCAGCAGCAGCATTTGTTACAGT
  CTCCAGTAAGCCATCATGAGCCCGAGTCACCTCATCTTAGATATTTGCCACCTCAGACTGTTGATCATAT
  AAACCAAGAGCATGAAGGGGATCTTGAGCCCCAAACACATGATGTGGATAAAAGTCTCCAAGATGATACA
  GAGCCTGAAAACGGATCTGATATTTCCAGTGCAGAAAATGAACAGACAGAAGCTGATCAGGCAACTGCAG
  CTGAAACATTATCTAAAAATGATATCTTGTATGATGGAGACTATGAAGAAAAGCCACTTGATATTGTCCA
  GAGCATTGTAGAAGAAATGGTGAACATCATTGTTGGAGACATGGGAGAAGGGATGGCTATCAGTGCAAGT
  ACAGAAGGCAACACTGGAACTGTAGAAGATGGTAGTGACAGTGAAAACATCCAAGCAAATGGAATCCCAG
  GAACACCCATTTCTGTTGCATATACACCATCGTTACCTGACGACAGGTTATCTGTCTCCTCCAATGATAC
  TCAGGAATCTGGAAATTCTTCAGGACCTTCACCTGGTGCTAAGTTTTCCCACATTTTACAAAAGGATGCC
  TTTCTAGTGTTCAGGTCATTGTGTAAGCTGTCAATGAAACCACTGTCAGATGGACCACCAGATCCAAAGT
  CTCATGAGTTACGGTCCAAGATTCTTTCATTGCAGTTGCTTCTGTCCATTCTGCAGAATGCAGGACCTGT
  TTTCAGGACAAATGAGATGTTTATTAATGCTATCAAGCAGTATCTATGTGTTGCACTCTCAAAAAACGGA
  GTCTCATCTGTTCCAGAGGTTTTTGAGCTTTCTCTTTCCATATTTCTTACTTTGTTGTCAAACTTCAAGA
  CACATCTGAAGATGCAAATTGAGGTATTCTTTAAAGAAATCTTCCTGTACATTTTGGAAACGTCTACAAG
  CTCATTTGACCATAAGTGGATGGTTATTCAGACACTGACAAGGATCTGTGCAGATGCTCAGAGTGTGGTG
  GACATTTATGTAAATTATGACTGTGACCTAAATGCAGCAAATATATTTGAAAGACTAGTAAATGACCTGT
  CGAAAATTGCCCAAGGAAGGGGCAGCCAAGAACTTGGTATGAGTAATGTTCAGGAATTGAGCCTGCGAAA
  AAAGGGGTTAGAATGTTTAGTTTCAATTTTGAAATGTATGGTTGAATGGAGCAAGGATCAGTATGTGAAC
  CCCAACTCTCAAACTACCCTTGGTCAGGAAAAACCCTCAGAGCAAGAGATAAGTGAAGTTAAACACCCAG
  AGACTATAAACAGATATGGAAGTTTAAACTCCTTAGAATCCACATCATCATCAGGAATTGGCAGCTACAG
  TACACAGATGTCTGGCACTGATAATCCAGAACAATTTGAGGTCCTAAAGCAGCAAAAAGAAATAATAGAA
  CAAGGGATTGATTTATTTAATAAGAAACCAAAGAGAGGAATACAATACCTCCAAGAACAAGGGATGCTTG
  GCACCACACCCGAAGATATAGCTCAGTTCTTACATCAAGAAGAAAGATTAGACTCTACTCAGGTGGGTGA
  GTTCCTGGGAGATAATGATAAATTCAATAAAGAAGTCATGTATGCATATGTAGACCAACATGACTTTTCA
  GGAAAAGACTTTGTTTCAGCTCTTCGTCTATTTCTGGAAGGATTCCGGCTTCCAGGAGAAGCTCAGAAAA
  TTGATCGATTAATGGAAAAATTTGCTGCAAGATACCTAGAGTGCAACCAAGGACAAACCCTTTTTGCTAG
  TGCAGATACTGCTTATGTTTTGGCCTACTCAATTATCATGTTGACTACAGACCTTCACAGTCCACAGGTT
  AAAAACAAAATGACAAAAGAACAGTACATTAAAATGAATCGTGGTATCAATGACAGTAAAGATCTCCCTG
  AAGAGTATCTATCAGCCATTTATAACGAAATAGCTGGGAAAAAAATATCAATGAAAGAAACAAAAGAACT
  AACAATCCCTACAAAGTCAACTAAACAGAATGTAGCCAGTGAAAAGCAAAGACGACTTCTGTATAACTTA
  GAAATGGAACAGATGGCCAAGACGGCTAAAGCACTCATGGAAGCTGTGAGCCATGTTCAGGCACCTTTTA
  CAAGTGCAACACACTTGGAGCACGTGAGGCCGATGTTTAAGTTGGCTTGGACACCTTTTCTGGCTGCATT
  CAGTGTGGGTCTCCAAGACTGTGATGATACTGAAGTAGCCTCTCTTTGCCTAGAAGGGATAAGATGTGCT
  ATCAGGATTGCATGCATTTTCAGCATTCAGCTGGAAAGAGATGCATATGTCCAAGCACTAGCCAGATTTA
  CCTTACTTACAGTGAGTTCTGGTATTACTGAAATGAAACAGAAGAATATCGACACAATAAAAACACTCAT
  CACGGTGGCTCACACTGATGGAAATTATTTAGGAAATTCGTGGCATGAGATCCTGAAGTGCATCAGTCAG
  TTAGAGCTGGCACAACTTATAGGAACTGGAGTGAAGCCTCGGTACATTTCTGGGACTGTGAGAGGCAGAG
  AAGGGTCTCTCACTGGGACAAAAGATCAGGCTCCAGATGAATTTGTTGGTCTAGGGCTAGTTGGAGGAAA
  TGTGGACTGGAAGCAGATAGCCAGTATTCAGGAGTCTATTGGAGAAACCAGCTCTCAAAGTGTGGTAGTT
  GCTGTGGATAGAATATTCACCGGATCCACAAGGCTAGATGGAAATGCTATTGTTGATTTTGTCCGTTGGC
  TATGTGCTGTGTCTATGGATGAATTGCTTTCTACTACACACCCAAGAATGTTTAGTCTACAAAAAATAGT
  AGAAATATCATACTATAACATGGGAAGAATAAGACTGCAGTGGTCTCGGATTTGGGAAGTTATTGGAGAT
  CATTTTAATAAGGTTGGTTGCAATCCTAATGAAGATGTAGCCATATTTGCAGTAGACTCTCTGAGGCAGC
  TATCAATGAAGTTTTTAGAGAAAGGAGAGCTTGCAAACTTCAGATTCCAAAAGGATTTCTTACGACCTTT
  TGAACATATAATGAAACGAAACAGGTCTCCAACAATTCGAGATATGGTTGTACGGTGTATAGCACAAATG
  GTTAATTCTCAGGCTGCAAATATTCGTTCAGGATGGAAGAACATTTTCTCAGTATTCCATCTAGCTGCAT
  CAGACCAAGATGAAAGCATAGTAGAACTTGCATTTCAGACAACAGGGCACATTGTCACTCTTGTATTTGA
  AAAACACTTTCCGGCCACCATTGATTCTTTCCAGGATGCAGTGAAGTGTTTGTCTGAATTCGCATGTAAT
  GCAGCTTTCCCAGACACAAGTATGGAAGCAATCCGACTTATTCGCCATTGTGCAAAATATGTGTCTGATA
  GACCGCAGGCTTTCAAGGAGTACACAAGTGATGACATGAGCGTGGCACCTGAAGACAGGGTGTGGGTGAG
  AGGGTGGTTCCCGATCCTCTTTGAGTTGTCTTGTATCATCAATAGATGCAAATTAGATGTAAGAACCAGG
  GGCTTAACAGTAATGTTTGAAATAATGAAAACATATGGCCACACTTATGAAAAACATTGGTGGCAGGACT
  TATTCAGAATTGTTTTCAGAATCTTTGACAACATGAAATTGCCAGAACAGCAGACAGAGAAAGCTGAATG
  GATGACAACAACTTGTAACCATGCCCTTTATGCAATATGTGATGTATTCACCCAGTATTTAGAAGTTCTC
  AGCGATGTACTTTTGGATGATATTTTTGCCCAGCTTTACTGGTGTGTACAACAAGACAATGAGCAGTTAG
  CACGATCTGGTACAAACTGCTTAGAAAACGTGGTTATTCTGAATGGTGAAAAGTTTACTCTTGAAATCTG
  GGATAAAACGTGTAACTGCACCCTGGATATCTTCAAAACCACAATTCCTCACGCGCTTCTGACTTGGCGT
  CCCACCTCTGGCGAGGCCGAACCCCCATCTCCATCAGCTGTGAGTGAAAAGCCGTTGGATGCAATATCAC
  AAAAATCTGTAGACATACATGATTCTATTCAACCAAGATCTTCAGATAACAGACAGCAAGCACCACTGGT
  TTCTGTTTCTACTGTGAGTGAAGAAGTTAGCAAAGTGAAATCCACAGCAAAATTCCCAGAACAAAAATTG
  TTTGCTGCCCTGTTGATTAAATGTGTTGTGCAGCTGGAACTCATCCAGACCATCGACAACATTGTCTTCT
  TCCCAGCCACAAGCAAGAAGGAGGATGCAGAGAACTTAGCTGCAGCCCAGAGAGATGCAGTAGACTTCGA
  TGTTCGAGTTGATACTCAAGACCAAGGAATGTACCGCTTTTTAACATCACAACAACTGTTCAAGCTACTG
  GACTGCTTATTAGAGTCGCATAGATTTGCAAAAGCATTTAATTCCAACAATGAACAGAGGACTGCTCTGT
  GGAAAGCAGGCTTCAAAGGCAAGTCCAAGCCCAATCTTCTAAAACAGGAGACAAGCAGCCTGGCGTGTGG
  GCTGCGCATTCTCTTCCGGATGTACATGGACGAGAGCAGAGTCAGTGCCTGGGAAGAAGTCCAGCAGAGG
  CTTTTAAACGTCTGCAGGGAAGCGCTGAGCTACTTCCTCACTCTAACATCCGAAAGTCACCGGGAAGCCT
  GGACTAACTTACTGTTACTGTTCCTCACTAAAGTTCTGAAGATCAGTGACAGCAGGTTTAAAGCTCACGC
  ATCATTCTACTACCCTCTCTTATGTGAAATTATGCAATTTGACTTGATTCCGGAACTGCGTGCTGTTCTT
  AGAAGATTTTTCCTGCGAATTGGAATAGTATTTCAAATATCACAACCACCTGAACAGGAACTTGGGATAA
  ACAGGCAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001102430
• Insert Size: 5541 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102430.1, NP_001095900.1
• RefSeq Size: 7042 bp
• RefSeq ORF: 5541 bp
• Locus ID: 211673
• UniProt ID: G3X9K3
• Cytogenetics: 1 A2
• Gene Summary: Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).[UniProtKB/Swiss-Prot Function]