Psme4 (NM_134013) Mouse Untagged Clone

CAT#: MC224972

Psme4 (untagged) - Mouse proteasome (prosome, macropain) activator subunit 4 (Psme4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Psme4
• Synonyms: AA409398; AU041366; mKIAA0077; TEMO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224972 representing NM_134013
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTCCGGAGCGGGCGGGCGGCGGGGAGCCCCCCGAGCCTGGCGGGCGGCCGGTACTGGGCCCGC
  GGGCCTTCGTCCCGCAGAAGGAGATCGTGTACAACAAGCTTCTGCCCTATGCCGAGCGGCTGGACGCCGA
  GTCCGACTTGCAGCTGGCACAGATCAAGAGCAACCTGGGCCGGGCCGTGCAGCTCCAAGAGCTGTGGCCC
  GGGGGCCTCTTCTGGACGCGGAAACTCTCCACTTATATCCGACTTTATGGGAGAAAATTTAGCAAAGAAG
  ATCATGTTCTTTTTATTAAGTTACTGTATGAGCTGGTATCAATTCCAAAACTAGAAATCAGCATGATGCA
  GGGATTTGCCCGTCTCTTGATCAACTTGTTAAAAAAAAAGGAACTTCTTTCAAGAGATGATTTAGAGTTA
  CCTTGGAGACCACTTTATGACTTGGTAGAAAGAATATTATATTCCAAGACAGAGCACCTGAGATTAAATT
  CATTTCCTAATTCTATAGAAAATGTTCTCAAAACACTGGTGAAAAGCTGCCGACCATACTTTCCAGCAGA
  TTCAACAGCAGAAATGTTAGAAGAATGGCGGCCTTTAATGTGTCCTTTTGATGTAACAATGCAAAAGGCC
  ATCAGTTATTTTGAAATATTTCTTCCTACCTCCCTTCCTCCAGAACTGCATCATAAAGGTTTTAAACTTT
  GGTTTGATGAATTAATTGGCCTTTGGGTTTCAGTGCAAAATCTCCCACAGTGGGAGGGGCAATTAGTAAA
  TCTCTTTGCTCGATTGGCTACAGATAATATAGGATACATAGATTGGGATCCATATGTACCGAAGATATTT
  ACAAGAATTCTGAGAAGCCTGAACCTCCCAGTGGGAAGCAGTCAAGTTTTAGTCCCAAGATTTTTAACAA
  ATGCTTATGATATAGGACATGCTGTAATATGGATCACCGCAATGATGGGTGGACCAAGTAAATTAGTACA
  AAAACATTTAGCTGGTTTGTTTAACAGCATCACATCATTTTATCATCCTTCAAATAATGGGCGTTGGCTG
  AACAAGTTAATGAAACTCCTTCAGCGGTTGCCAAACAGTGTTGTTAGGAGATTACATCGTGAAAGATTCA
  AGAAGCCCTCCTGGTTAACTCCTGTGCCTGAAAGCCATAAGCTTACTGATGAAGATGTTACAGACTTTGT
  ACAATGCATTATTCAGCCTGTCCTCTTGGCTATGTTTAGCAAAACTGGTAGTCTAGAAGCAGCCCAGGCT
  CTGCAGAACCTTGCACTTATGAGACCTGAGTTAGTAATACCCCCTGTGCTTGAAAGAACATACCCTGCTT
  TAGAGACATTAACAGAACCTCACCAGCTCACAGCTACTTTAAATTGTGTAATTGGAGTAGCCCGAAGTTT
  GGTATCAGGCAGCAAATGGTTTCCCGAAGGGCCAACACACATGCTACCTCTGTTGATGAGAGCATTGCCT
  GGGGTAGATCCAAACGACTTTAGTAAGTGCATGATCACATTCCAATTTATAGGAACATTTTCTACTCTGG
  TGCCTTTAGTAGACTGTTCATCTGTACTGCAAGAAAGAAATGACCTCACAGAAATTGAAAAAGAACTTTG
  TTCGGCAACAGCTGAATTTGAGGATTTTGTGTTACAATTTATGGACAGATGCTTTGGACTAATAGAAAGT
  AGTACATTGGAGCAAACAAGGGAAGAGACAGAAACAGAGAAAATGACTCATTTGGAGAGTTTGGTTGAAT
  TAGGCTTATCTTCTACGTTTAGTACAATCCTCACACAATGTTCCAAAGACATATTTATGGTGGCCCTTCA
  GAAGGTCTTTAACTTTTCTGTTTCACATATATTTGAAACAAGAGTTGCAGGTCGCATGGTGGCAGACATG
  TGCCGAGCTGCTGTGAAGTGTTGCCCAGAAGAATCCCTGAAGCTGTTTGTTCCCCACTGCTATGGTGTTA
  TAACTCAGCTTACAATGAATGATGATGTATTGAATGAAGAAGAGCTAGACAAGGAGTTGCTGTGGAACCT
  TCAGCTTCTGTCTGAGATTACTCGTGTAGACGGGAAGAAGTTACTTCTGTATAGGGAGCAGCTTGTAAAG
  ATTCTCCAGCGAACCTTACACCTGACCTGTAAGCAGGGCTACACGCTGTCTTGCAACCTTCTGCATCATC
  TTCTTCGTTCTACCACACTAATCTACCCTACAGAGTACTGCAGTGTTCCGGGGGGCTTTAACAAGCCGCC
  TTCTGAATACTTCCCTGTCAAGGACTGGGGTAAGCCTGGAGATTTGTGGAATCTGGGGATCCAGTGGCAT
  GTTCCTTCTTCAGAAGAAGTCTCTTTTGCCTTCTATTTGTTAGACTCCTTTCTTCAGCCTGAGCTTATCA
  AACTCCAGTGTTGTGGAGATGGAGAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGT
  ACACAGCTGCTTGATTGGTTCAGGGAACCTTCTACCTCCATTGAAAGGAGAGGCAGTTACTAACTTGGTA
  CCAAGTATGGTCTCCTTAGAGGAAACAAAATTGTATACCGGACTGGAACATGACCTATCCAGAGAAAACT
  ACCGAGAAGTAATTGCTAGTGTCATAAGGAAACTTCTTAGCCACATACTTGATAATTCAGAAGATGATAC
  CAAATCACTGTTTCTCATCATAAAGATCATTGGAGACCTTCTGCACTTCCAAGGATCTCATAAACATGAA
  TTTGACTCTCGATGGAAAAGCTTTAACTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGAAAAAAGC
  AACATATCAGAGCATTACTAATTGACAGAGTGATGCTGCAGCATGAGTTGCGAACACTAACTGTTGAGGG
  TTGTGAGTACAAAAAAATACATCAAGATATGATCAGAGATCTTCTTCGATTATCAACAAGCTCATACAGT
  CAGGTCAGAAACAAAGCTCAGCAAACATTTTTTGCTGCTTTGGGGGCATATAACTTCTGTTGCAGAGATA
  TTATCCCCTTGGTTTTGGAATTCCTACGGCCTGATAGAAAGGATGTCACACAGCAGCAGTTCAAGGGCGC
  CTTATATTGTCTACTTGGAAATCACAGTGGTGTGTGCCTGGCAAACCTTCATGATTGGGACTGTATTGTG
  CAGACCTGGCCAGCACTTGTTTCTTCAGGGCTTAGCCAAGCAATGTCACTGGAAAAGCCATCAATAGTGA
  GACTATTTGATGACCTTGCTGAAAAGATTCATAGACAATATGAAACAATTGGCTTGGATTTTACAATTCC
  AAAGTCATGTGCTGCAATAGCAGAATTACTTCAACAATCAAAGAATCCTTCCATTAGCCAGACACTACTT
  AGCCCAGAAAAGATTAAAGAAGGACAGAAGCGTCAACAAGATAAGAATGCTGATGCCCTAAGGAACTATG
  AATGTTTGGTAAATACTTTACTAGATGGTGTGGAGCAAAGAAATCTGCCTTGGAAATTTGAGCATATAGG
  AATCGGGCTTCTGTCTCTACTCCTAAGGGATGACAGAGTGTTGCCTCTTCGTGCCATACGTTTTTTTGTT
  GAAAATCTCAACCATGATGCAATTGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGC
  AGCTAAAGAGAACTCACAAAAAGCTGACTATCAACCCTTATGAAATCAGTGGATGTCCTAAACCTACCAA
  AATTCTTGCTGGTGATAGACCTGATAACCATTGGTTGCATTATGATAGTAAAAATATACCAAGAACAAAA
  AAAGAATGGGAGTCAAGTTGCTTTGTTGAAAAAACTCACTGGGGATACTACAACTGGCCAAAGAATATGG
  TTGTTTATGCTGGTGTGGAAGAACAGCCTAAACTTGGCAGAAGCAGGGAGGATATGATAGAGGCAGAACA
  GATTATATATGATCGTTTTTCTGATCCTAAGTTTGTTGAGCAGTTAATCACATTTCTATCTTTAGAAGAC
  AGAAAAGGAAGAGATAAGTTTAGTCCCCGGCGTTTCTGCCTCTTTAAGGGTATATTTAGAAATTTTGATG
  ATGCTTTTCTGCCTGTTCTGAAGCCCCATTTAGAACGTTTGGTTGCAGATTCTCATGAAAGTACCCAGAG
  ATGTGTTGCAGAAATTATAGCTGGTTTAATCAGAGGTTCTAAGCATTGGACGTTTGAGAAGGTGGAGAAG
  CTTTGGGAGCTCCTGTGCCCGCTGCTGAGAACGGCACTGTCCAACATGACAGTGGAGACATACAATGACT
  GGGGGACCTGTATAGCCACTTCCTGTGAAAGCAGAGATCCCCGGAAGCTTCACTGGCTTTTTGAGCTGCT
  CTTGGAGTCACCATTGAGTGGTGAAGGGGGGTCCTTTGTAGATGCATGCCGACTCTATGTGCTACAAGGT
  GGCCTTGCCCAGCAAGAGTGGAGAGTCCCTGAACTATTGCACAGACTTCTGAAGTACTTGGAACCAAAAC
  TCACCCAGGTTTACAAAAATGTCAGAGAAAGAATAGGAAGTGTGCTGACCTACATATTTATGATAGATGT
  TTCTTTGCCAAATACTGCTCCAACAACATCTCCTTGTATCCCTGAGTTTACTGCTCGCGTCCTAGAAAAA
  CTGAAACCACTCACAGATGTGGATGAAGAAATTCAGAACCATGTTATGGAAGAAAATGGCATTGGTGAAG
  AAGATGAACGAACTCAGGGCATTAAACTCTTAAAAACTATATTGAAATGGCTTATGGCAAGTGCAGGAAG
  ATCTTTTTCTACAGCAGTCAAAGAGCAACTTCAGCTTCTGCCTTTGTTCTTTAAGATCGCTCCAGTGGAA
  AATGACAATAGTTATGATGAACTGAAAAGAGATGCAAAGCTCTGTTTGTCACTAATGTCTCAGGGGTTGC
  TGTACCCTCAACAAGTGCCTTTGATACTTCAGGTGCTAAGCCAGACAGCAAGAAGCAGTTCTTGGCATGC
  AAGATATACAGTGCTGACCTACCTCCAGACCATGGTATTTTATAACCTCTTTATTTTCCTAAACAATGAA
  GATGCAGTTAAAGACATCAGATGGCTGATTATATGCCTTTTGGAGGATGAACAGTTAGAGGTTCGAGAAA
  TGGCTGCTACCACCTTAAGTGGTCTGTTACAGTGTAACTTCCTTACCATGGACAGTGCTATGCAGATTCA
  TTTTGAGCAACTTTGCAAAACAAAACTACCCAAGAAAAGAAAACGAGACCCTGGTTCTGTGGGAGATACC
  ATTCCTTCTGCAGAGTTGGTCAAACGTCATGCTGGTGTGCTTGGACTTGGTGCATGTGTTCTTTCTAGCC
  CTTATGATGTTCCTACCTGGATGCCCCAGCTCCTTATGAACCTCAGTGCACATTTGAATGATCCTCAGCC
  TATTGAGATGACTGTAAAGAAGACCTTATCCAATTTCCGAAGGACACATCATGACAACTGGCAAGAACAC
  AAGCAGCAGTTCACTGATGACCAGCTGCTTGTCCTCACTGACCTCCTTGTGTCACCATGCTATTATGCAT
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134013
• Insert Size: 5532 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134013.3, NP_598774.2
• RefSeq Size: 6454 bp
• RefSeq ORF: 5532 bp
• Locus ID: 103554
• UniProt ID: Q5SSW2
• Cytogenetics: 11 A4
• Gene Summary: Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.[UniProtKB/Swiss-Prot Function]