Scn4a (NM_133199) Mouse Untagged Clone

CAT#: MC224966

Scn4a (untagged) - Mouse sodium channel, voltage-gated, type IV, alpha (Scn4a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Scn4a
• Synonyms: mH2; Nav1.4; SkM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224966 representing NM_133199
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCTCATCTCTGCCCACCCTGGTCCCCCCTGGTCCCCACTGCCTGCGCCCCTTCACCCCAGAGT
  CCCTGGCAGCCATAGAACAGCGGGCAATGGAGGAGGAAGCCCGGCTGCAGCGGAACAAGCAAATGGAGAT
  TGAAGAGCCTGAGCGGAAGCCACGCAGTGACCTGGAAGCTGGCAAGAACCTCCCACTCATCTATGGGGAC
  CCCCCACCTGAAGTCATTGGCGTCCCCCTGGAGGACCTGGACCCTTACTACAGTGACAAGAAGACCTTCA
  TTGTGCTCAACAAAGGAAAGGCCATCTTCCGATTCTCTGCCACCCCTGCCCTCTACATGCTGAGCCCCTT
  CAGCATCGTCCGGCGGGTGGCTATCAAGGTGCTCATCCACGCGCTGTTCAGCATGTTCATCATGATCACC
  ATCTTGACCAACTGCGTGTTCATGACCATGAGCAACCCGCCTTCTTGGTCCAAGGACGTGGAGTACACCT
  TCACAGGGATCTACACCTTTGAGTCCCTCATTAAGATGCTGGCCCGAGGCTTTTGCATTGATGACTTCAC
  ATTCCTCCGAGACCCCTGGAACTGGCTGGACTTCAGTGTCATCACAATGGCGTACGTGACAGAGTTTGTG
  GACTTGGGCAACATCTCAGCCCTGAGGACCTTCCGTGTGCTGCGGGCCCTGAAAACCATCACGGTCATCC
  CAGGGCTGAAGACGATCGTGGGAGCCCTGATCCAGTCTGTGAAAAAGCTCTCGGATGTGATGATCCTCAC
  TGTCTTCTGCCTGAGCGTCTTTGCCCTGGTGGGGCTGCAGCTTTTCATGGGAAACCTGCGTCAGAAGTGC
  GTGCGTTGGCCCCCGCCCATGAATGACACCAACACCACGTGGTATGGTAATGATACGTGGTACGGCAATG
  ACACCTGGTACGGCAATGACACCTGGTATGGCAATGACACTTGGAACAGCCAGGAGAGCTGGGTCAGCAA
  CTCTACCTTTGACTGGGAGGCCTACATCAATGACGAAGGGAACTTCTATTTCTTGGAGGGCTCCAATGAT
  GCCCTGCTCTGTGGGAATAGCAGTGATGCCGGGCACTGCCCTGAGGGCTACGAATGCATGAAGGCTGGGA
  GGAACCCCAACTATGGCTACACCAGCTACGACACCTTCAGCTGGGCCTTCCTGGCTCTCTTCCGTCTCAT
  GACGCAGGACTACTGGGAGAACCTTTTCCAGCTGACCCTTCGAGCCGCTGGCAAGACCTACATGATCTTC
  TTTGTGGTCATCATCTTCCTGGGTTCCTTCTACCTCATCAATCTGATCCTGGCGGTGGTGGCCATGGCAT
  ATGCTGAGCAGAATGAGGCTACCCTGGCCGAAGACCAGGAGAAAGAGGAGGAGTTCCAACAGATGCTTGA
  AAAATTCAAAAAACATCAGGAGGAACTGGAAAAGGCGAAGGCTGCCCAGGCTCTGGAAGGTGGAGAGGAG
  GCAGATGGGGACCCAACCCACAGCAAAGACTGCAATGGTAGCCTGGATACATCCGGGGAGAAGGGGCCCC
  CAAGGCCAAGCTGCAGCGCAGAGAGTGCCATCTCAGATGCTATGGAGGAGCTGGAAGAGGCCCATCAGAA
  GTGCCCCCCATGGTGGTACAAGTGTGCACACAAAGTGCTCATCTGGAACTGCTGTGCCCCGTGGGTGAAG
  TTCAAGCATATTATCCTCCTGATTGTCATGGACCCCTTTGTGGACCTGGGCATCACCATCTGCATCGTGC
  TCAACACCCTCTTCATGGCCATGGAGCACTACCCCATGACTGAGCACTTTGACAACGTGCTCTCTGTGGG
  CAACTTGGTCTTCACGGGCATCTTCACTGCAGAGATGGTGCTGAAGTTGATTGCTATGGACCCCTACGAA
  TATTTCCAACAGGGCTGGAACATCTTCGACAGCTTCATCGTCACCCTCAGCCTGGTGGAGCTGGGCCTGG
  CCAACGTGCAGGGCCTATCAGTGCTCCGTTCTTTCCGTCTGCTGCGTGTCTTCAAGCTGGCCAAGTCATG
  GCCAACACTCAACATGCTCATCAAAATCATTGGCAACTCAGTGGGTGCGCTGGGCAACCTGACCCTGGTG
  TTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGCATGCAGCTGTTTGGCAAGAGCTATAAGGAATGCG
  TGTGCAAGATTGCCTCAGACTGCAGCCTGCCCCGCTGGCACATGCACGATTTCTTCCACTCCTTCCTCAT
  CGTCTTCCGCATCCTCTGTGGGGAGTGGATCGAGACCATGTGGGACTGCATGGAAGTGGCCGGCCAGGCC
  ATGTGCCTCACCGTCTTCCTCATGGTCATGGTCATTGGCAACCTGGTGGTCCTGAATCTGTTCCTGGCTC
  TCCTGTTGAGTTCCTTCAGCGCTGACAGTCTGGCAGCGTCAGATGAAGATGGTGAGATGAACAACCTACA
  GATCGCCATCGGGCGTATCAAGTGGGGCATCGCCTTTGCCAAAACCTTCCTCTTGGGGCTGTTGCATGGC
  AAGATCCTGAGCCTCAAAGACATAATGCTCAGTCTCGGGGAGCCTGGGGGTGCTGGGGAAAACGGGGAGA
  GCCCTCCCGAGGATGAGAAGAAGGAACCACCTCCGGAAGATGGGAACAAGGAGCTGAAAGACAATCATAT
  CCTGAACCACGTGGGCCTGACTGATGGACCCCGCTCCAGCATCGAGATGGACCACCTTAACTTCATCAAC
  AACCCCTACCTCACTATCCATGTGCCCATTGCCTCCGAGGAGTCCGACTTGGAAATGCCCACAGAGGAGG
  AGACTGACACCTTCTCGGAGCCTGAGGACATCAAGAAGCCACTACAGCCTCTGTACGACGGGAACTCCTC
  CGTCTGCAGCACAGCTGACTACAAGCCCCCCGAAGAGGACCCTGAGGAGCAGGCTGAGGAGAACCCTGAG
  GGGGAGCTACCTGAGGAGTGTTTCACAGAGGCCTGTGTGAAGCGCTGCCCCTGCCTCTATGTGGACATCT
  CCCAGGGCCGCGGGAAAATGTGGTGGACACTCCGCAGGGCCTGCTTCAAGATTGTTGAGCACAACTGGTT
  TGAGACCTTCATCGTCTTCATGATCTTGCTCAGCAGCGGGGCCCTGGCCTTCGAGGACATCTACATTGAA
  CAGCGGCGAGTCATCCAAACCATCCTGGAATATGCTGACAAGGTCTTCACCTACATCTTCATCCTGGAGA
  TGCTGCTAAAGTGGGTGGCCTACGGCTTCAAGGTGTACTTCACCAACGCCTGGTGCTGGCTCGACTTCCT
  CATTGTGGATGTCTCCATCATCAGTCTGGTGGCTAACTGGCTGGGCTACTCTGAGCTGGGGCCCATCAAA
  TCCCTGCGCACGCTCCGGGCCCTGCGACCTCTGAGGGCACTGTCCCGATTTGAAGGCATGAGGGTGGTGG
  TGAACGCCCTTCTGGGAGCCATCCCTTCCATCATGAACGTGCTCCTTGTCTGCCTCATCTTCTGGCTCAT
  CTTCAGCATCATGGGGGTCAACTTGTTTGCTGGAAAGTTCTACTACTGCATCAACACAACCACCTCCGAG
  AGATTCGACATCTCTGTGGTCAACAACAAGTCCGAGTGTGAGAGCCTCATGTACACCGGCCAGGTCCGCT
  GGATGAACGTCAAGGTCAACTATGACAACGTGGGTCTCGGCTACCTCTCTCTTCTTCAGGTGGCCACTTT
  CAAGGGTTGGATGGACATCATGTATGCAGCTGTGGACTCCCGGGAGAAAGAGGAGCAGCCAGACTACGAG
  GTGAACCTCTACATGTACCTTTACTTCGTCATCTTTATCATCTTCGGCTCCTTCTTCACCCTCAATCTCT
  TCATTGGCGTCATCATTGACAACTTCAACCAACAGAAGAAGAAGTTTGGAGGGAAAGACATCTTCATGAC
  AGAGGAACAGAAGAAATACTACAATGCGATGAAGAAGCTTGGCTCCAAGAAGCCCCAGAAGCCAATCCCC
  CGGCCTCAGAACAAGATCCAAGGCATGGTGTACGACTTCGTGACAAAGCAGGTGTTTGACATCTCTATCA
  TGATCCTCATCTGCCTCAACATGGTGACGATGATGGTGGAGACGGATGACCAGAGCCAGCTCAAGGTGGA
  CATCCTGTATAACATCAACATGGTCTTCATTATCGTCTTCACAGGGGAGTGTGTGCTCAAGATGTTTGCC
  CTGCGCCATTACTACTTCACCATTGGCTGGAATATCTTCGACTTCGTGGTTGTCATCTTGTCCATTGTGG
  GTCTCGCACTCTCTGACTTGATACAGAAATACTTTGTGTCACCCACACTGTTCCGTGTGATCCGATTGGC
  TCGGATCGGGCGTGTCTTGCGTCTGATCCGTGGGGCCAAGGGCATCCGGACGCTGCTGTTCGCCCTGATG
  ATGTCCCTGCCCGCCCTCTTCAACATCGGCCTCCTTCTCTTTCTGGTCATGTTCATTTACTCCATCTTCG
  GCATGTCTAACTTCGCCTACGTCAAGAAAGAGTCGGGCATCGACGACATGTTCAACTTTGAGACCTTTGG
  CAACAGTATCATCTGCCTCTTCGAGATCACCACGTCAGCTGGCTGGGACGGGCTTCTGAACCCCATCCTC
  AACAGTGGGCCCCCAGACTGTGACCCGACATTGGAGAACCCAGGCACCAATATCAAGGGTGACTGCGGCA
  ACCCATCCATCGGCATCTGTTTCTTCTGCAGCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTA
  CATTGCCATCATCCTAGAGAATTTCAATGTGGCCACTGAAGAGAGCAGTGAGCCCCTCTGTGAGGATGAC
  TTCGAGATGTTCTATGAGACCTGGGAGAAGTTTGACCCCGATGCAACTCAGTTCATCGACTACAGCCGCC
  TTTCTGACTTTGTGGACACCCTGCAGGAGCCCTTGAAAATTGCCAAGCCCAATAAGATCAAGCTTATCAC
  ATTAGACCTGCCCATGGTACCCGGGGACAAGATCCACTGCCTGGACATCCTCTTTGCCCTGACCAAAGAG
  GTACTGGGTGACTCTGGGGAAATGGATGCTCTCAAACAGACCATGGAGGAGAAGTTCATGGCAGCTAACC
  CCTCCAAGGTCTCCTATGAGCCCATCACCACCACCCTCAAGAGGAAGCAGGAGGAGGTGTGTGCTATCAA
  AATCCAGAGGGCTTACCGCCGCCATCTGCTGCAGCGCTCTGTGAAGCAGGCCTCTTACATGTACCGCCAC
  AGCCAGGAAGGCAATGGCGATGGGGCCCCTGAGAAGGAGGGATTGCTTGCCAACACCATGAACAAGATGT
  ATGGCTCTGAGAAAGAGGACAACGGTGTGCAGAGCCAGGGGGAGAAAGAGAAAGACTCAACAGAGGATGC
  TGGACCCACCACGGAGGTCACAGCCCCCAGCAGCTCGGACACTGCCCTGACTCCTCCTCCTCCTTCTCCT
  CCTCCACCATCATCACCACCACAAGGGCAGACAGTGCGCCCAGGGGTCAAAGAGTCTCTTGTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133199
• Insert Size: 5526 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133199.2, NP_573462.2
• RefSeq Size: 6598 bp
• RefSeq ORF: 5526 bp
• Locus ID: 110880
• UniProt ID: Q9ER60
• Cytogenetics: 11 68.91 cM
• Gene Summary: Pore-forming subunit of a voltage-gated sodium channel complex through which Na(+) ions pass in accordance with their electrochemical gradient. Alternates between resting, activated and inactivated states (PubMed:11834499). Required for normal muscle fiber excitability, normal muscle contraction and relaxation cycles, and constant muscle strength in the presence of fluctuating K(+) levels (PubMed:18317596, PubMed:21881211).[UniProtKB/Swiss-Prot Function]