Afdn (NM_010806) Mouse Untagged Clone

CAT#: MC224949

Mllt4 (untagged) - Mouse myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 4 (Mllt4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Afdn
• Synonyms: 5033403D15Rik; Af-6; AF6; Afadin; Gm314; I-afadin; Mllt4; S-afadin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224949 representing NM_010806
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGGGCGGCCGCGACGAAGAGCGGCGGAAGCTGGCCGACATCATTCACCACTGGAACGCCAACC
  GGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTTCATGGAGTCATGAGATTTTATTT
  TCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGAGTCTCTAGCACAGCCACCACTCAAGAT
  GTGATTGAAACACTGGCAGAGAAGTTCCGCCCTGACATGCGCATGCTCTCCTCTCCCAAGTACTCCCTCT
  ATGAAGTGCATGTCAGTGGAGAAAGAAGATTGGACATTGATGAGAAACCTCTAGTTGTACAGTTGAATTG
  GAACAAAGATGATCGAGAGGGTCGATTCGTCCTTAAGAACGAGAATGACGCCATTCCTGCCAAGAAGGCT
  CAGAGTAATGGACCTGAAAAGCAGGAGAAAGAAGGTGTTATTCAGAACTTCAAAAGAACTCTCTCAAAGA
  AAGAAAAGAAGGAAAAAAAGAAGAAAGAGAAGGAGGCATTGAGACAAGCCTCTGATAAGGAAGAAAGGCC
  TTCTCAAGGGGATGACAGTGAGAACTCACGACTGGCTGCTGAAGTTTACAAAGACATGCCTGAGACCAGC
  TTTACTAGGACCATTTCTAACCCTGAAGTTGTCATGAAACGGCGGCGGCAGCAAAAACTGGAGAAGAGAA
  TGCAAGAGTTTCGGAGCTCAGATGGGCGGCCTGACTCAGGTGGAACACTGAGGATATATGCAGATAGTTT
  AAAACCAAATATTCCATACAAGACAATCCTGCTGTCTACTACCGATACCGCAGACTTTGCTGTAGCCGAA
  TCTTTGGAGAAGTATGGTCTGGAAAAAGAGAATCCCAAGGACTACTGTATTGCCCGGGTTATGCTTCCTC
  CTGGAGCCCAGCATTCTGATGAGAGAGGTGCTAAGGAAATAATTCTTGACGATGATGAATGTCCTTTACA
  AATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGACCACCGGACTAC
  ATCCCAAAGAAAATGAAGAAACATGTTGAAGGGAAGTCGTTGAAGGGGAAAGACAGAGCTGACGGGTCTG
  GCTACGGCTCCGCTCTTCCTCCCGAGAAGCTGCCCTACTTAGTAGAGCTAAGCCCAGGGAGAAGGAATCA
  CTTTGCCTACTACAGCTATCACACTTACGAAGATGGCTCTGACTCCAGAGACAAGCCAAAGTTATACCGC
  CTTCAATTAAGTGTGACTGAAGTTGGGACAGAGAAATTTGATGACAATTCTATCCAGCTGTTTGGCCCCG
  GAATTCAGCCCCATCACTGTGATCTCACCAACATGGATGGAGTGGTCACTGTGACACCCAGAAGTATGGA
  TGCAGAGACCTACGTGGATGGTCAGCGCATCTCAGAGACCACAATGCTGCAGAGTGGCATGAGACTGCAG
  TTTGGCACCTCGCATGTGTTTAAGTTTGTGGACCCCATCCAGGACCACGTTCTTTCCAAGAGATCTGTGG
  ATGGAGGCCTGATGGTCAAGGGCCCAAGACATAAACCTGGAGCTGTTCAGGAGACAACCTTTGAATTGGG
  AGGAGATGTCCACAGTGGGACAGCATTGCCGGCAAGCAGGAGCACTACTAGACTGGACAGTGACAGGGTG
  TCCTCTGCCTCTAGCACAGCTGAGCGAGGGATGGTGAAGCCGATGATCCGACTGGACCAGGAGCAGGAAT
  ATCGACGACGAGAAAACAGGACTCAGGATGCTACTGGGCCTGAACTGATACTGCCTGCCAGCATTGAATT
  CAGGGAAAGCTCTGAAGACTCTTTTTTATCTGCCATTATAAACTATACCAATAGTTCTACAGTTCATTTT
  AAGTTGTCACCTACATATGTGTTATACATGGCATGCCGGTATGTATTGTCCAGCCAGCACAGGCCCGACA
  TCAGTCCTACAGAGCGTACACATAAGGCCATTGCTGTTGTCAACAAGATGGTGAGCATGATGGAAGGGGT
  CATCCAGAAACAGAAGAACATTGCAGGAGCACTTGCTTTTTGGATGGCAAATGCATCTGAGCTTCTCAAC
  TTCATCAAGCAGGACCGAGACCTTAGTCGGATCACGCTGGATGCCCAGGATGTCCTAGCACACTTGGTTC
  AGATGGCATTTAAGTACTTGGTTCATTGCCTTCAGTCAGAACTTAATAACTACATGCCAGCCTTTCTGGA
  TGATCCTGAGGAGAACAGTCTGCAAAGACCAAAAATAGATGATGTGCTGCACACACTCACAGGAGCCATG
  TCCTTGCTGCGGCGCTGCAGAGTCAATGCTGCCTTGACCATCCAGCTCTTCTCTCAGCTTTTCCACTTCA
  TCAATATGTGGCTGTTCAACAGACTGGTGACTGACCCAGATTCAGGCCTGTGCTCCCACTACTGGGGTGC
  CATTATCCGCCAGCAGCTGGGGCATATTGAAGCGTGGGCAGAGAAGCAGGGGCTAGAGCTGGCAGCTGAC
  TGTCACTTGAGCAGAATTGTACAGGCTACAACATTGCTTACGATGGATAAGTATGTACCTGATGACATTC
  CAAATATAAACAGCACATGCTTTAAGTTAAATTCTCTGCAGCTTCAAGCCTTGTTACAAAATTACCACTG
  TGCACCCGATGAGCCTTTTATTCCTACGGATCTCATAGAAAATGTTGTGGCTGTTGCTGAGAACACAGCT
  GATGAGCTCGCCCGCAGTGATGGCAGAGACGTGCAGCTGGAGGAAGACCCAGACCTACAGCTGCCTTTTC
  TTCTGCCAGAAGATGGCTATTCCTGTGATGTTGTCAGAAACATTCCAAATGGTTTACAAGAATTTTTAGA
  CCCTCTGTGCCAGAGAGGATTTTGTAGATTGGTTCCTCACACACGTTCACCAGGTACCTGGACAATATAT
  TTTGAAGGAGCAGATTATGAAAGTCACCTTATGCGTGAGAACGCAGAACTGGCCCAGCCTCTGAGGAAGG
  AGCCTGAGATAATCACTGTGACTCTGAAGAAGCAGAATGGAATGGGCCTCAGCATCGTTGCAGCCAAGGG
  TGCTGGCCAGGATAAACTGGGAATCTACGTTAAGTCGGTTGTCAAAGGAGGTGCTGCAGATGTGGATGGA
  CGATTAGCTGCTGGTGACCAACTCCTCAGTGTGGATGGACGAAGTCTGGTTGGACTTTCTCAGGAAAGGG
  CAGCAGAACTCATGACAAGAACCAGTTCCGTGGTTACACTGGAAGTTGCAAAGCAAGGTGCCATCTATCA
  CGGCCTGGCCACCCTCCTTAACCAGCCGTCCCCCATGATGCAAAGAATTTCAGATCGTCGTGGCTCAGGT
  AAACCCAGACCAAAGAGTGAAGGTTTTGAGCTCTATAATAATTCAGCTCAAAATGGCTCCCCAGAGAGTC
  CTCAGATGCCTTGGACAGAGTACAGTGAGCCAAAGAAACTGCCTGGGGATGACAGGCTGATGAAAAACAG
  AGCGGACCACCGTTCCAGCCCCAATGTGGCAAATCAGCCTCCTAGCCCTGGAGGGAAGGGTCCATATACA
  TCTGGAACAGCAGCTAAGATAACATCTGTCTCCACTGGAAACCTCTGCACTGAGGAGCAGTCCCCTCCAC
  CCAGACCTGAAGCCTATCCTATCCCTACTCAGACATACACCAGAGAGTATTTTACCTTTCCAGCTTCAAA
  GTCGCAGGATCGGATGGCTCCTCCTCAGAGCCAGTGGCCAAATTATGAGGAAAAACCACATGTGCACACA
  GAAAGTAATCATTCCAGTATTGCAATCCAGCGTGTTACCCGTTCCCAGGAAGAGCTTCGGGAAGAAAAGG
  TTTACCAACTTGAGCGGCATCGAGTAGAGGCAGGCATGGATCGCAAGTGTGACAGTGATATGTGGATCAA
  TCAGAGCTCCTCAGTGGAATCCAGCACATCCAGCCAGGAACACCTGAACCATTCTTCCAAGTCAGTTACC
  CCTGCTTCCACTCTGACCAAGAGTGGTCCTGGCCGTTGGAAAACACCAGCAGCGGTGCTGCCCACCCCTG
  TGGCTGTCTCTCAGCCCATTCGAACAGACCTCCCTCCACCACCACCTCCACCGCCGGTCCACTACACCAG
  TGAGTTTGATGGTATTCCAATGGATTTGCCCCTCCCACCTCCTCCTGCCAACCAGGCAGGACCCCAGTCT
  GCTCAGGTGGCTGCTGCAGAGTGGAAAAAGAGAGAGGAGCACCAGAGGTGGTATGAGAAAGAGAAGGCCC
  GCCTGGAGGAAGAACGAGAGAGAAAGCGCAGGGAGCAGGAGCGGAAGTTGGGGCAGATGCGCTCTCAGAC
  CCTGAACCCTGCTTCCTTCTCACCTTTGGCCACACAGGCCAAGCCAGAAAAGCCTTCCACACTGCAGAGG
  CCCCAAGAAACAGTCATTCGGGAGCTTCAGCCCCAGCAGCAGCCCCGCACGATCGAGCGCAAGGATCTGC
  AGTATATAACCATCAGTAAAGAGGAGCTGTCCTCTGGTGATAGTCTGTCTCCAGACCCCTGGAAACGAGA
  TGCCAGGGAGAAGCTGGAGAAGCAACAGCAAATGCACATTGTGGACATGCTAAGCAAGGAGATCCACGAG
  CTGCAAAACAAGGTGGACCGTACTGCGGAGGAGAGTGACCGCCTGCGGAAGCTTATGCTGGAGTGGCAGT
  TCCAGAAGAGACTACAGGAGTCCAAACAGAAGGATGAGGATGATGACGAAGAGGAAGATGATGATGTGGA
  CACCATGCTGATCATGCAGCGTCTGGAGGCCGAGCGGAGAGCCAGGATGCAGGATGAGGAGCGCAGGCGC
  CAGCAACAGTTGGAAGAGATGCGGAAACGAGAAGCAGAAGATCGAGTAAGACAAGAGGAAGATGGACGCC
  ATCAGGAGGAGGAGCGAGTAAAGAGAGATGCTGAAGAAAAGAGGCGACAGGAGGAAGGGTATTACAGCCG
  CCTAGAAGCTGAGAGGCGCCGACAGCACGAAGAGGCAGCACGCAGGCTGCTGGAGCCTGAAGAGCCCGGG
  CTGAGCCGACCTCCACTTCCACGGGACTATGAGCCCCCGTCCTTGTCCTCAGCACCCTGTGCCCCTCCTC
  CCCCACCTCAGCGAAACGCATCCTACCTCAAAACACAGGTCCTCTCCCCAGACTCGCTGTTCACTGCCAA
  GTTTGTTGCGTATGATGAGGAGGAGGAGGACTACGGCCCAGCAGGACCAAACTCTTACTCGGGATCTGCA
  GGCACAGCTGTGGGAGCCTATGATGCCCCTCGGGAAGCGAGAGAGAAACTGACTAGGAGCCAAGACGCAG
  ACTTACCTGGCAGTTCTGGAGCCCCTGAAAATCTGACATTTAAAGAGCGTCAGCGCCTTTTTTCACAAGG
  GCAGGATGTGTCTGACAAAGTGAAAGCTTCTCGTAAATTAACAGAGCTCGAGAATGAACTGAACACAAAG
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010806
• Insert Size: 5463 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010806.1, NP_034936.1
• RefSeq Size: 7401 bp
• RefSeq ORF: 5463 bp
• Locus ID: 17356
• UniProt ID: Q9QZQ1
• Cytogenetics: 17 A1- A2
• Gene Summary: Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm.[UniProtKB/Swiss-Prot Function]