Brca1 (NM_009764) Mouse Untagged Clone

CAT#: MC224945

Brca1 (untagged) - Mouse breast cancer 1 (Brca1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Brca1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224945 representing NM_009764
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTATCTGCCGTCCAAATTCAAGAAGTACAAAATGTCCTTCATGCTATGCAGAAAATCTTAGAGT
  GTCCGATCTGTTTGGAACTGATCAAAGAACCTGTTTCCACAAAGTGTGACCACATATTTTGCAAATTTTG
  TATGCTGAAACTTCTTAACCAGAAGAAAGGGCCTTCACAATGTCCTTTGTGTAAGAATGAGATAACCAAA
  AGGAGCCTACAGGGAAGCACAAGGTTTAGTCAGCTTGCTGAAGAGCTGCTGAGAATAATGGCTGCTTTTG
  AGCTTGACACGGGAATGCAGCTTACAAATGGTTTTAGTTTTTCAAAAAAGAGAAATAATTCTTGTGAGCG
  TTTGAATGAGGAGGCGTCGATCATCCAGAGCGTGGGCTACCGGAACCGTGTCAGAAGGCTTCCCCAGGTC
  GAACCTGGAAATGCCACCTTGAAGGACAGCCTAGGTGTCCAGCTGTCTAACCTTGGAATCGTGAGATCAG
  TGAAGAAAAACAGGCAGACCCAACCTCGAAAGAAATCTGTCTACATTGAACTAGACTCTGATTCTTCTGA
  AGAGACAGTAACTAAGCCAGGTGATTGCAGTGTGAGAGACCAGGAATTGTTACAGACCGCCCCTCAAGAA
  GCTGGAGATGAAGGCAAGCTGCACTCTGCAGAAGAGGCTGCTTGTGAGTTTTCTGAGGGCATAAGAAACA
  TTGAACATCATCAATGCAGTGATGATTTAAACCCTACTGAGAATCATGCAACTGAAAGGCATCCAGAAAA
  ATGTCAGAGTATTTCTATTTCAAATGTGTGTGTGGAGCCATGTGGCACAGATGCTCATGCCAGCTCATTA
  CAGCCTGAGACCAGCAGTTTATTGCTCATTGAAGACAGAATGAATGCAGAAAAGGCTGAATTCTGTAATA
  AAAGCAAACAGCCTGGCATAGCAGTGAGCCAGCAGAGCAGATGGGCTGCAAGTAAAGGAACATGTAACGA
  CAGGCAGGTTCCCAGCACTGGGGAAAAGGTAGGTCCAAACGCTGACTCCCTTAGTGATAGAGAGAAGTGG
  ACTCACCCGCAAAGTCTGTGCCCTGAGAATTCTGGAGCTACCACCGATGTTCCTTGGATAACACTAAATA
  GCAGCGTTCAGAAAGTTAATGAGTGGTTTTCCAGAACTGGTGAAATGTTAACTTCTGACAGCGCATCTGC
  CAGGAGGCACGAGTCAAATGCTGAAGCAGCTGTTGTGTTGGAAGTTTCAAACGAAGTGGATGGGGGTTTT
  AGTTCTTCAAGGAAAACAGACTTAGTAACCCCCGACCCCCATCATACTTTAATGTGTAAAAGTGGAAGAG
  ACTTCTCCAAACCAGTAGAGGATAATATCAGTGATAAAATATTTGGGAAATCCTATCAGAGAAAGGGAAG
  CCGCCCTCACCTGAACCATGTGACTGAAATTATAGGCACATTTATTACAGAACCACAGATAACACAAGAG
  CAGCCCTTCACAAATAAATTAAAACGTAAGAGAAGTACATCCCTTCAACCTGAGGACTTCATCAAGAAAG
  CAGATTCAGCAGGTGTTCAAAGGACTCCTGACAACATAAATCAGGGAACTGACCTAATGGAGCCAAATGA
  GCAAGCAGTGAGTACTACCAGTAACTGTCAGGAGAACAAAATAGCAGGTAGTAATCTCCAGAAAGAGAAA
  AGCGCTCATCCAACTGAATCATTGAGAAAGGAACCTGCTTCCACAGCAGGAGCCAAATCTATAAGCAACA
  GTGTAAGTGATTTGGAGGTAGAATTAAACGTCCACAGTTCAAAAGCACCTAAGAAAAATAGGCTGAGGAG
  GAAGTCTTCTATCAGGTGTGCTCTTCCACTTGAACCAATCAGTAGAAATCCAAGCCCACCTACTTGTGCT
  GAGCTTCAAATCGATAGTTGTGGTAGCAGTGAAGAAACAAAGAAAAACCATTCCAACCAACAGCCAGCCG
  GGCACCTTAGAGAGCCTCAACTCATCGAAGACACTGAACCTGCAGCGGATGCCAAGAAGAACGAGCCAAA
  TGAACACATAAGGAAGAGACGTGCCAGCGATGCTTTCCCAGAAGAGAAATTAATGAACAAAGCTGGTTTA
  TTAACTAGCTGTTCAAGTCCTAGAAAATCTCAAGGGCCTGTCAATCCCAGCCCTCAGAGAACAGGAACAG
  AGCAACTTGAAACACGCCAAATGTCTGACAGTGCCAAAGAACTCGGGGATCGGGTCCTAGGAGGAGAGCC
  CAGTGGCAAAACCACTGACCGATCTGAGGAGAGCACCAGCGTATCCTTGGTATCTGACACTGACTACGAC
  ACTCAGAACAGTGTCTCAGTCCTGGACGCTCACACTGTCAGATATGCAAGAACAGGATCCGCTCAGTGTA
  TGACTCAGTTTGTAGCAAGCGAAAACCCCAAGGAACTCGTCCATGGCTCTAACAATGCTGGGAGTGGCAC
  AGAGGGTCTCAAGCCCCCCTTGAGACACGCGCTTAACCTCAGTCAGGAGAAAGTAGAAATGGAAGACAGT
  GAACTTGATACTCAGTATTTGCAGAATACATTTCAAGTTTCAAAGCGTCAGTCATTTGCTTTATTTTCAA
  AACCTAGAAGTCCCCAAAAGGACTGTGCTCACTCTGTGCCCTCAAAGGAACTGAGTCCAAAGGTGACAGC
  TAAAGGTAAACAAAAAGAACGTCAGGGACAGGAAGAATTTGAAATCAGTCACGTACAAGCAGTTGCGGCC
  ACAGTGGGCTTACCTGTGCCCTGTCAAGAAGGTAAGCTAGCTGCTGATACAATGTGTGATAGAGGTTGTA
  GGCTTTGTCCATCATCTCATTACAGAAGCGGGGAGAATGGACTCAGCGCCACAGGTAAATCAGGAATTTC
  ACAAAACTCACATTTTAAACAATCAGTTTCTCCCATCAGGTCATCTATAAAAACTGACAATAGGAAACCT
  CTGACAGAGGGACGATTTGAGAGACATACATCATCAACTGAGATGGCGGTGGGAAATGAGAACATTCTTC
  AGAGTACAGTGCACACAGTTAGCCTGAATAACAGAGGAAATGCTTGTCAAGAAGCCGGCTCGGGCAGTAT
  TCATGAAGTATGTTCCACTGGTGACTCCTTCCCAGGACAACTAGGTAGAAACAGAGGGCCTAAGGTGAAC
  ACTGTGCCTCCATTAGATAGTATGCAGCCTGGTGTCTGTCAGCAAAGTGTTCCTGTAAGTGATAAGTATC
  TTGAAATAAAAAAGCAGGAGGGTGAGGCTGTCTGTGCAGACTTCTCTCCATGTCTATTCTCAGACCATCT
  TGAGCAATCTATGAGTGGTAAGGTTTTTCAGGTTTGCTCTGAGACACCTGATGACCTGCTGGATGATGTT
  GAAATACAGGGACATACTAGCTTTGGTGAAGGTGACATAATGGAGAGATCTGCTGTCTTTAACGGAAGCA
  TCCTGAGAAGGGAGTCCAGTAGGAGCCCTAGTCCTGTAACCCATGCATCGAAGTCTCAGAGTCTCCACAG
  AGCGTCTAGGAAATTAGAATCGTCAGAAGAGAGCGACTCCACTGAGGATGAAGATCTTCCCTGCTTCCAA
  CACTTACTGAGCAGAATAAGCAACACACCTGAGCTTACCAGATGCAGCAGTGCTGTGACACAGCGTATGC
  CAGAGAAAGCGGAGGGGACCCAAGCACCATGGAAGGGTAGCAGCAGTGACTGCAATAATGAGGTGATCAT
  GATAGAGGCATCTCAGGAGCATCAGTTTAGTGAGGATCCAAGATGCTCTGGCAGCATGTTCTCTTCACAG
  CACAGTGCTGCCCAAGGGTCAACTGCAAATGCAAACTCCCAGGATTCCAATTTTATTCCACCTTCCAAAC
  AGAGGAGTCACCAGTGTGGGAATGAGGAAGCTTTCCTAAGTGACAAGGAATTGATTTCAGATAACGAGGA
  AATGGCAACTTGCCTAGAAGAGGATAATGACCAAGAAGAGGATAGTATAATCCCAGATTCAGAGGCATCC
  GGATACGAGAGTGAAACAAACCTTTCTGAAGACTGCTCGCAGAGTGATATTTTAACCACTCAGCAGCGGG
  CGACCATGAAGTATAACCTGATAAAGCTGCAGCAGGAAATGGCTCACCTGGAAGCTGTGCTGGAGCAGCG
  TGGGAACCAGCCTTCTGGCCACTCCCCTTCCCTCCTAGCGGACCCTTGTGCCCTGGAAGACCTGCCAGAT
  CTGGAACCAAACATGTCAGGAGCAGCAATTTTAACTTCAAAGAACATTAATGAGAATCCTGTAAGCCAAA
  ATTTGAAGAGCGCTTGTGATGACAAATTCCAACTACAACATCTGGAGGGTCCCACCAGTGGAGATGACGA
  GTCAGGGATGGGAAGGCCTTCCCCTTTTAAATCTCCGTTGGCAGGCAGTAGGGGCTCTGCACATGGCTGC
  TCTAGGCATCTTCAAAAGAGAAACTCCCCCTCTCAGGAGGAGCTCCTCCAGCCTGCTGGATCAGAGGCGT
  CATCTGAGCCACACAATTCAACAGGGCAGTCTTGCCTGCCAAGGCGAGAGCTAGAAGGAACCCCATACCT
  GGGATCTGGAATCAGCCTTTTCTCTAGTAGAGACCCCGAATCTGAGTCCCCTAAAGAGCCAGCCCACATT
  GGCACCACACCAGCTTCAACCTCTGCACTGAAAATACCCCAAGGTCAAGTTGCTTTCCGGAGTGCAGCTG
  CTGCTGGTGCTGATAAAGCAGTGGTAGGAATTGTGAGCAAGATAAAGCCGGAATTGACATCTTCAGAAGA
  AAGAGCGGATAGAGACATATCCATGGTGGTGTCAGGCTTGACCCCCAAAGAAGTAATGACCGTGCAAAAG
  TTTGCTGAAAAATACCGCCTCACTTTAACTGACGCAATTACTGAGGAGACTACACATGTAATTATAAAAA
  CAGATGCGGAGTTTGTGTGTGAGCGGACACTGAAATATTTTCTGGGCATTGCAGGAGGAAAGTGGATAGT
  TAGCTATTCATGGGTGGTCCGGTCTATCCAAGAAAGAAGACTTCTGAATGTGCATGAATTTGAAGTCAAA
  GGAGATGTTGTGACTGGAAGAAATCACCAAGGTCCAAGGCGATCCAGAGAATCCCGGGAAAAGCTCTTCA
  AGGGCCTACAGGTCTATTGTTGTGAGCCCTTCACCAACATGCCCAAAGATGAGCTGGAGAGGATGCTGCA
  GCTGTGTGGGGCTTCCGTGGTGAAGGAGCTTCCATCGCTCACCCATGACACAGGTGCTCATCTAGTTGTG
  ATCGTGCAGCCAAGCGCCTGGACAGAAGACAGCAACTGCCCAGATATTGGGCAGCTGTGCAAGGCACGTC
  TTGTGATGTGGGACTGGGTGTTGGACAGTCTATCCAGCTACCGGTGTCGGGATCTGGATGCCTACCTGGT
  ACAGAATATCACCTGTGACAGTAGTGAGCCACAAGACTCCAATGATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009764
• Insert Size: 5439 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009764.3, NP_033894.3
• RefSeq Size: 6648 bp
• RefSeq ORF: 5439 bp
• Locus ID: 12189
• UniProt ID: P48754
• Cytogenetics: 11 D
• Gene Summary: E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (By similarity). Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.[UniProtKB/Swiss-Prot Function]