Arfgef2 (NM_001085495) Mouse Untagged Clone

CAT#: MC224934

Arfgef2 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arfgef2
• Synonyms: AI463430; AW493672; BIG2; E230011G24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224934 representing NM_001085495
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGAAAGCCAGACCAAGAGCATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCTGACAAGGAGG
  TGAAGCGGCCCCAGCACTCCCAGCTGCGTAGGGCCTGCCAGGTGGCGCTCGATGAAATTAAAGCAGAACT
  AGAAAAGCAGAGGCTTGGCGCTGCGGCTCCACCAAAGGCAAACTTCATTGAAGCTGACAAGTATTTCCTT
  CCATTCGAGCTGGCCTGCCAATCCAAGTCACCTCGGGTGGTCAGCACATCCCTGGACTGCTTGCAGAAAC
  TCATCGCATATGGACACATCACTGGCAATGCCCCTGACAGTGGCGCTCCTGGGAAGCGGCTGATCGACAG
  GATCGTTGAAACCATTTGCAATTGTTTTCAGGGCCCTCAGACAGATGAAGGGGTTCAGCTACAAATCATT
  AAGGCTCTGCTGACTGCTGTGACATCCCCACACATTGAAATCCATGAGGGCACCATCCTGCAGACAGTGA
  GGACCTGCTACAATATCTATCTGGCCAGCAAAAACCTCATCAATCAAACCACCGCCAAGGCCACCCTTAC
  CCAAATGCTGAATGTCATCTTCACCCGCATGGAAAACCAAGTGTTACAGGAGGCCAGAGAATTGGAAAAA
  CCAATGCAGTCAAAACCTCAGTCCCCGGTGATCCAGGCCACAGCAGGCTCTCCCAAGTTCAGCCGCCTGA
  AGCAGAGCCAGGCCCAGAGCAAACCCACGACTCCTGAGAAAGCAGAGCTGCCCAATGGGGACCATGCCCA
  GAGTGGCCTAGGAAAAGTGAGCTTGGAAAATGGAGAAGCTCCCCGAGAGAGAGGCTCGCCAGTCTCTGGG
  AGAGCCGAGCCTTCGAGGGGCACTGACAGTGGAGCTCAGGAGGTGGTGAAGGACATCTTAGAGGACGTGG
  TCACGTCTGCTGTGAAAGAAGCAGCAGAAAAGCATGGTCTGCCGGAGCCCGACCGAGCCCTGGGTGCCCT
  AGAGTGCCAGGAATGCGCTGTTCCTCCTGGAGTTGATGAAAACTCACAGACCAACGGCATAGCAGACGAC
  AGGCAGTCGCTATCCTCCGCAGATAATCTGGAACCAGATGTTCAAGGACACCAAGTGGCTGCTAGGTTCT
  CTCACATTCTACAGAAGGATGCCTTCCTGGTGTTCCGCTCCCTGTGCAAGCTGTCCATGAAGCCCCTTGG
  GGAAGGGCCACCAGATCCAAAGTCCCACGAGCTTCGTTCCAAGGTGGTTTCCCTGCAGCTGCTCCTCTCT
  GTGCTGCAGAATGCTGGCCCAGTCTTCAGGAGCCACGAGATGTTCGTCACTGCCATTAAGCAGTACCTGT
  GTGTGGCCCTTTCCAAGAATGGCGTGTCCTCAGTGCCTGACGTCTTCGAACTCTCGCTTGCTATTTTTCT
  TACTCTTCTTTCAAACTTCAAGATGCACTTGAAAATGCAGATAGAGGTCTTTTTCAAAGAGATTTTCCTG
  AACATTTTAGAAACATCCACAAGTTCTTTTGAGCACCGCTGGATGGTCATTCAGACCCTGACGAGGATTT
  GTGCAGATGCCCAGTGCGTTGTGGATATTTATGTCAACTACGACTGTGACTTAAATGCTGCTAACATTTT
  CGAGCGACTAGTAAATGACTTATCCAAAATTGCTCAGGGAAGAAGTGGACATGAGCTGGGGATGACACCT
  CTACAGGAGCTCAGTCTGAGGAAGAAAGGCCTGGAGTGTCTGGTGTCTATTCTCAAGTGCATGGTGGAGT
  GGAGCAAGGACCTGTATGTGAATCCCAACCACCAGGCTACCCTGGGTCAGGAGAGGCTCCCAGATCAGGA
  AATGGGGGACGGAAAAGGCCTTGACATGGCAAGACGGTGCAGCGTGACATCCGTGGAGTCCACAGTGTCC
  TCGGGGACCCAGACAGCCATTCAGGATGACCCAGAGCAGTTTGAGGTCATCAAACAACAGAAGGAAATCA
  TTGAGCATGGCATTGAGCTCTTCAATAAGAAGCCCAAGCGAGGTATCCAGTTTCTCCAAGAGCAAGGCAT
  GCTGGGAGCAGCTGTAGAAGATATTGCCCAATTTCTGCACCAGGAGGAGCGGCTGGATTCTACCCAGGTT
  GGCGAGTTTCTGGGAGACAGCACGAGGTTTAACAAGGAGGTGATGTATGCCTATGTGGACCAACTGGATT
  TCTGTGAAAAAGAGTTCGTCTCAGCCCTGAGGACATTCCTGGAGGGCTTCCGCCTGCCTGGGGAAGCCCA
  GAAGATTGACCGGTTAATGGAGAAGTTTGCTGCACGATACATAGAATGTAACCAAGGGCAAACCCTGTTT
  GCTAGTGCGGACACTGCGTATGTCCTGGCGTACTCCATCATCATGCTGACCACAGACCTGCACAGCCCAC
  AGGTAAAGAATAAGATGACAAAAGAGCAGTATATTAAAATGAACCGGGGCATCAACGACAGCAAAGACCT
  GCCTGAGGAGTACCTGTCCAGCATCTATGACGAGATAGAGGGCAAGAAGATAGCGATGAAGGAGACAAAG
  GAGCACACAATTGCGACCAAGTCCACCAAGCAGAGTGTAGCTAGTGAAAAGCAGAGGCGGCTGCTGTACA
  ATGTGGAGATGGAGCAGATGGCTAAAACAGCCAAAGCTCTGATGGAGGCCGTGAGCCATGCCAAAGCCCC
  GTTTACCAGCGCCACACACTTGGACCATGTCCGGCCAATGTTCAAACTGGTGTGGACGCCGCTGCTGGCA
  GCCTATAGTATTGGCCTGCAGAACTGTGATGACACGGAGGTGGCCTCCCTGTGTTTGGAAGGCATCAGGT
  GTGCAGTCCGCATTGCCTGCATCTTTGGAATGCAGCTGGAACGAGATGCCTATGTTCAGGCCCTTGCTCG
  CTTTTCCCTGCTGACAGCCAGCTCCAGCATCACAGAAATGAAGCAGAAAAACATCGACACCATAAAGACA
  CTCATCACTGTGGCTCACACCGATGGCAACTACCTTGGCAACTCCTGGCATGAGATCTTGAAGTGCATCA
  GCCAGCTGGAGCTTGCTCAGCTGATAGGAACTGGGGTGAAGACTCGCTACCTCTCTGGCTCTGGGCGGGA
  ACGAGAAGGGAGCCTGAAGGGCCACAGTCTGGCAGGAGAAGAATTCATGGGTCTTGGCCTCGGTAACTTG
  GTGAGTGGTGGTGTAGACAAAAGACAGATGGCCAGCTTCCAGGAGTCGGTTGGTGAAACAAGCTCACAGA
  GTGTGGTTGTCGCAGTTGACAGAATTTTTACTGGCTCTACCAGACTGGATGGAAATGCAATAGTTGACTT
  TGTCCGTTGGCTGTGTGCTGTGTCCATGGATGAGCTGGCTTCTCCCCACCATCCTCGAATGTTCAGCCTG
  CAGAAGATTGTGGAAATATCATACTACAACATGAACAGGATCCGGCTGCAGTGGTCTCGGATATGGCATG
  TCATTGGAGATCACTTCAATAAGGTCGGCTGTAACCCCAATGAAGATGTGGCCATCTTCGCAGTTGACTC
  TCTGCGGCAGCTCTCCATGAAGTTCCTGGAGAAGGGAGAATTAGCCAACTTCCGATTTCAGAAGGATTTT
  CTGAGGCCATTTGAACATATTATGAAGAAAAACAGGTCCCCGACCATCCGGGACATGGTGATCCGATGCA
  TCGCTCAGATGGTGAGCTCCCAGGCAGCCAACATCCGCTCAGGCTGGAAGAACATCTTTGCTGTGTTCCA
  CCAGGCTGCCTCTGACCACGATGGGAACATTGTGGAGCTGGCCTTCCAAACCACAGGCCACATCGTCTCA
  ACCATCTTCCAGCACCACTTCCCTGCAGCCATCGACTCCTTCCAGGATGCTGTGAAGTGCTTGTCTGAGT
  TCGCCTGCAATGCAGCTTTCCCTGACACCAGCATGGAGGCCATCCGGCTCATCCGCTTCTGTGGGAAATA
  CGTCTCAGAGAGGCCTCGGGTGCTGCAAGAGTACACAAGTGATGATATGAATGTGGCTCCTGGTGACAGG
  GTCTGGGTCAGAGGCTGGTTTCCCATCCTGTTTGAACTCTCCTGCATCATTAACAGATGCAAGTTAGATG
  TACGCACAAGGGGACTCACGGTCATGTTTGAGATTATGAAGAGCTATGGCCACACCTTTGCAAAGCACTG
  GTGGCAGGACCTGTTCAGAATCGTGTTTCGAATTTTTGACAACATGAAACTCCCTGAGCAACAGTCAGAG
  AAATCAGAGTGGATGACGACCACGTGCAATCATGCACTGTACGCTATTTGTGACGTATTCACCCAGTTCT
  ACGAAGCTTTGCATGAAGTGCTTCTCTCTGATGTGTTTGCGCAGCTGCAGTGGTGCGTGAAACAAGATAA
  TGAGCAGTTGGCCCGATCAGGTACGAACTGCTTAGAGAACCTAGTGATATCCAACGGCGAGAAGTTCAGC
  CCTGCTGTCTGGGATGAAACCTGCAATTGCATGTTGGATATTTTCAAAACCACCATCCCACATGTTTTGC
  TGACGTGGAGACCTGCGGGGATGGAGGAGGAGGTGTCAGATAGACATCTGGATGTGGACCTAGACCGCCA
  GTCTTTAAGCAGCATAGACAGAAACGCCTCCGAGAGAGGACAGAGCCAGCTGTCCAACCCCACTGACGAC
  AGCTGGAAGGGGGCACCGTATGCACATCAGAAACTGCTGGCCAGCCTCCTCATCAAGTGTGTGGTTCAGC
  TGGAGCTGATCCAGACCATTGACAACATCGTGTTCTACCCTGCCACCAGCAAGAAGGAGGATGCGGAGCA
  CATGGTTGCTGCCCAGCAAGACACCCTCGATGCAGAGATCCACATCGAGACGGAGAATCAGGGCATGTAC
  AAGTTCATGTCCTCCCAGCACCTCTTCAAGCTGCTGGACTGCCTGCAGGAGTCCCACTCCTTCTCCAAAG
  CCTTCAACTCCAACTATGAGCAGCGGACTGTCCTCTGGCGAGCTGGCTTCAAGGGCAAGTCCAAACCCAA
  TCTTCTCAAACAAGAAACCAGCAGCCTGGCCTGTTGTCTAAGGATCCTGTTTAGAATGTACGTTGATGAG
  AACCGCAGGGATTCCTGGGACGAAATACAGCAGCGACTTCTAAGAGTGTGCAGTGAAGCACTTGCCTATT
  TCATTACTGTGAACTCCGAGAGCCATCGGGAAGCATGGACGAGCCTCCTGCTGCTACTTCTAACCAAAAC
  CCTCAAGATAAGCGATGAAAAGTTCAAAGCACACGCGTCAATGTACTACCCCTACCTGTGTGAAATTATG
  CAGTTTGACCTGATCCCTGAGCTCCGAGCAGTTCTGCGAAAGTTCTTCCTGCGGATAGGCCTGGTGTATA
  AGATATGGATTCCAGAAGAGCCGTCGCAAGTGCCAGCAGCACTGTCATCGACCTGGTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001085495
• Insert Size: 5379 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085495.2, NP_001078964.1
• RefSeq Size: 8777 bp
• RefSeq ORF: 5379 bp
• Locus ID: 99371
• UniProt ID: A2A5R2
• Cytogenetics: 2 H3
• Gene Summary: Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways (By similarity).[UniProtKB/Swiss-Prot Function]