Ubr2 (NM_001177374) Mouse Untagged Clone

CAT#: MC224909

Ubr2 (untagged) - Mouse ubiquitin protein ligase E3 component n-recognin 2 (Ubr2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubr2
• Synonyms: 9930021A08Rik; AI462103; AW540746; E130209G04Rik; ENSMUSG00000043296; mKIAA0349
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224909 representing NM_001177374
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGAGATGGAGCCCGAGGTGCAGGCCATCGACCGCAGTTTGCTGGAATGTTCTGCCGAAGAGA
  TCGCAGGGAGATGGCTGCAAGCAACCGACCTCAACAGAGAAGTGTACCAGCATTTAGCCCACTGTGTGCC
  CAAAATCTACTGCCGGGGCCCTAACCCCTTCCCTCAGAAGGAAGACACGCTGGCACAGCACATCCTGCTG
  GGACCGATGGAGTGGTACATCTGCGCTGAAGACCCTGCGCTGGGATTTCCAAAGCTCGAGCAGGCAAACA
  AGCCTTCTCACCTCTGTGGCCGAGTGTTTAAAGTGGGGGAACCTACATACTCCTGCAGAGACTGTGCAGT
  TGACCCCACCTGTGTTTTATGCATGGAGTGCTTCCTGGGAAGTATCCATAGAGACCATCGATATAGGATG
  ACCACATCGGGAGGAGGGGGCTTCTGTGACTGTGGTGACACTGAGGCGTGGAAAGAGGGACCTTACTGCC
  AGAAGCACAAGCTCAGCAGCTCTGAAGTTGTGGAGGAGGAGGATCCTCTTGTGCATCTATCAGAAGATGT
  GATCGCCAGAACTTACAACATTTTTGCTATTATGTTTCGATATGCAGTAGATATACTGACCTGGGAAAAA
  GAAAGTGAATTGCCTGAAGACTTAGAAGTGGCAGAGAAGAGTGACACCTACTACTGCATGCTGTTTAATG
  ATGAGGTTCACACCTATGAGCAAGTCATTTATACCCTTCAGAAAGCTGTGAACTGTACACAGAAGGAAGC
  CATTGGCTTTGCAACTACAGTTGATCGAGATGGCCGTAGGTCTGTCCGATATGGAGATTTCCAGTACTGT
  GATCAAGCAAAGACAGTCATTGTGAGGAACACCAGCAGACAGACCAAGCCGCTCAAAGTTCAAGTTATGC
  ACTCCTCCGTGGCTGCTCATCAGAATTTTGGTTTGAAAGCTCTGTCGTGGCTGGGAAGTGTTATTGGATA
  CTCAGATGGCCTTCGCAGGATTTTGTGTCAAGTTGGATTACAAGAAGGTCCAGATGGCGAAAACTCTTCT
  CTGGTCGACAGACTGATGCTTAATGATTCCAAATTATGGAAAGGGGCTAGGAGTGTGTATCACCAGTTGT
  TCATGAGCAGCCTGCTCATGGACCTCAAGTATAAGAAGCTGTTCGCGCTTCGATTTGCTAAAAACTATGA
  GCGTTTGCAGAGAGATTATGTGACAGATGACCACGACAGAGAGTTTTCAGTCGCAGACCTCTCGGTTCAG
  ATATTCACAGTTCCTTCACTTGCGCGAATGCTCCTCACAGAAGAGAACCTGATGACCGTTATCATTAAGG
  CTTTCATGGACCATTTGAAACACAGAGATGCCCAGGGCAGATTCCAGTTTGAACGCTACACTGCCCTCCA
  AGCCTTCAAGTTCAGGAGAGTCCAGAGCCTCATCTTAGATCTCAAGTATGTATTGATTAGCAAACCAACG
  GAGTGGTCAGATGAGCTGAGGCAGAAGTTCTTACAAGGGTTCGATGCCTTCTTGGAATTACTGAAGTGCA
  TGCAGGGAATGGACCCGATCACGCGTCAGGTGGGACAGCACATTGAGATGGAGCCAGAGTGGGAAGCAGC
  CTTCACACTGCAGATGAAGCTGACACACGTCATCTCAATGGTGCAGGACTGGTGTGCTCTGGACGAAAAA
  GTGTTAATTGAAGCTTACAAGAAATGCCTGGCTGTGCTGACACAGTGTCATGGCGGATTTACTGATGGTG
  AACAGCCAATCACACTCAGTATTTGTGGACACTCGGTGGAAACCATCAGATACTGTGTTTCCCAAGAAAA
  AGTTAGCATTCACCTCCCAATTTCTCGCTTGCTTGCAGGTTTGCATGTATTGTTAAGCAAAAGTGAAGTG
  GCATATAAATTTCCAGAGCTCCTACCTCTAAGTGAACTGAGCCCACCCATGTTGATAGAACATCCTCTTA
  GATGTCTTGTCTTATGTGCTCAAGTGCATGCTGGGATGTGGAGAAGAAATGGCTTCTCTCTAGTAAATCA
  GATCTATTACTACCATAATGTGAAATGCAGGCGAGAGATGTTCGACAAGGACATAGTGATGCTTCAGACA
  GGTGTCTCCATGATGGACCCAAACCACTTCCTGATGATCATGCTCAGCCGCTTTGAACTCTATCAGCTCT
  TCAGCACGCCTGACTATGGGAAGAGATTCAGTTCTGAGGTTACCCATAAGGACGTCGTTCAGCAGAACAA
  CACTCTGATCGAAGAGATGCTCTACCTCATCATCATGCTTGTGGGAGAAAGATTCAACCCTGGGGTTGGA
  CAGGTGGCTGCCACAGATGAAATCAAGAGGGAGATTATCCATCAGTTGAGCATCAAGCCTATGGCTCACA
  GTGAGCTGGTGAAGTCTCTGCCTGAAGATGAGAACAAGGAGACCGGCATGGAGAGCGTCATCGAGTCCGT
  TGCACATTTCAAGAAACCTGGGCTCACAGGGCGAGGCATGTATGAGCTGAAGCCAGAGTGTGCCAAAGAG
  TTCAACCTGTATTTTTATCATTTCTCCAGGGCAGAGCAGTCCAAGGCAGAGGAAGCTCAGCGGAAATTGA
  AAAGAGAAAATAAAGAAGATACAGCACTCCCTCCTCCGGCTTTGCCACCGTTCTGCCCTTTGTTCGCGAG
  TCTGGTTAACATCTTGCAGTGTGACGTCATGCTGTACATCATGGGAACGATCCTGCAGTGGGCTGTAGAG
  CATCACGGGTCTGCCTGGTCAGAGTCCATGCTACAGAGGGTGCTGCATTTGATCGGGATGGCTCTCCAGG
  AAGAGAAGCACCACTTGGAGAACGCCGTGGAAGGGCACGTGCAGACCTTCACCTTCACACAGAAGATTTC
  AAAGCCTGGTGATGCACCACATAACTCCCCGAGCATCCTAGCTATGCTGGAGACCTTGCAGAACGCCCCC
  TCCCTGGAAGCCCACAAGGACATGATCAGGTGGTTGCTAAAGATGTTTAATGCAATTAAGAAGATAAGAG
  AGTGTTCATCCAGCAGCCCTGTGGCCGAGGCGGAGGGAACCATAATGGAGGAGAGCTCAAGAGACAAGGA
  CAAAGCAGAGAGGAAAAGAAAAGCCGAGATCGCCAGACTGCGCCGGGAGAAGATCATGGCCCAGATGTCT
  GAGATGCAGCGGCACTTCATTGACGAAAACAAAGAGCTCTTCCAGCAGACCCTAGAGCTGGACACCTCTG
  CCTCTGCCACTCTTGACAGCAGCCCTCCCGTTTCAGACGCAGCTCTTACAGCACTGGGCCCAGCACAGAC
  ACAGGTCCCTGAACCGAGACAGTTTGTCACCTGTATATTATGTCAAGAGGAGCAAGAGGTGACTGTGGGA
  AGCAGGGCGATGGTCTTGGCAGCGTTTGTTCAGAGGTCAACGGTTCTGTCAAAAGACAGGACGAAAACCA
  TCGCGGACCCAGAAAAATATGATCCATTATTCATGCACCCCGATCTGTCTTGTGGGACACACACTGGCAG
  CTGTGGGCACGTTATGCATGCCCATTGTTGGCAAAGGTATTTTGATTCCGTTCAAGCCAAGGAGCAGCGA
  AGGCAGCAGCGGCTGCGCTTGCACACTAGCTACGATGTAGAGAATGGCGAGTTCCTCTGCCCGCTCTGTG
  AGTGCCTGAGCAACACGGTGATCCCCCTGCTGCTTCCTCCCAGGAGCATCCTCAGCAGGAGGTTAAATTT
  TTCAGACCAACCAGATCTGGCACAGTGGACGAGAGCAGTAACACAGCAGATAAAGGTGGTCCAGATGCTG
  AGGAGAAAGCACAATGCTGCTGACACGTCTTCTTCAGAGGACACAGAAGCCATGAATATAATACCGATCC
  CCGAAGGCTTCAGGCCTGATTTTTATCCTAGGAACCCATATTCTGATAGCATAAAAGAAATGTTAACGAC
  ATTTGGAACGGCTGCTTACAAGGTGGGACTGAAGGTTCATCCTAATGAAGGTGACCCCCGTGTGCCCATC
  CTGTGCTGGGGGACCTGTGCATACACCATCCAGAGCATAGAAAGAATTTTGAGTGATGAGGAGAAGCCTG
  TTTTTGGACCTCTGCCTTGTAGACTGGACGACTGTCTCAGGTCGTTAACACGGTTTGCAGCAGCACATTG
  GACAGTGGCGTTACTTCCTGTGGTACAAGGACACTTCTGTAAACTCTTTGCATCCTTGGTGCCTAGTGAC
  AGCTATGAAGACCTCCCGTGCATACTAGACATCGACATGTTTCACTTGCTGGTGGGCCTGGTGCTCGCTT
  TCCCAGCTCTGCAGTGTCAGGATTTTTCAGGAAGCAGCCTGGCCACTGGGGACCTGCACATCTTCCACTT
  GGTTACCATGGCACACATCGTACAGATCTTACTTACCTCATGTACAGAAGAGAATGGCATGGATCAAGAG
  AATCCCACTGGGGAAGAAGAACTGGCCATTCTCTCTTTGCACAAAACACTTCACCAGTATACTGGAAGTG
  CCTTGAAAGAAGCCCCCTCCGGCTGGCACCTGTGGAGGAGCGTCCGGGCCGCCATCATGCCTTTCCTCAA
  GTGCTCTGCTTTGTTTTTCCACTATTTAAATGGAGTCCCGGCCCCTCCAGACCTTCAAGTTTCTGGAACA
  AGCCATTTTGAACACTTATGTAACTACCTTTCCCTACCAACCAACCTCATTCACCTTTTTCAAGAAAACA
  GTGACATCATGAACTCCCTGATTGAAAGTTGGTGCCAGAACAGTGAAGTTAAACGGTATCTAAATGGCGA
  GAGAGGAGCGATAAGCTACCCCAGAGGAGCTAACAAACTGATAGACCTTCCAGAGGATTACAGCAGCCTC
  ATTAACCAAGCATCCAATTTCTCGTGCCCCAAATCAGGTGGCGACAAGAGCAGAGCTCCTACTCTGTGCC
  TCGTGTGTGGGAGTCTCCTCTGCTCTCAGAGTTACTGCTGCCAAGCTGAGCTGGAGGGTGAGGACGTCGG
  AGCCTGCACAGCACACACCTACTCCTGCGGCTCCGGGGCCGGCATCTTCCTGAGAGTGCGGGAATGTCAG
  GTGCTATTTTTAGCTGGCAAAACCAAAGGATGTTTTTATTCTCCTCCTTACCTTGACGACTATGGAGAGA
  CCGACCAGGGACTCAGACGAGGAAATCCTTTACATTTATGCCAAGAGCGGTTTCGAAAGATCCAGAAGCT
  CTGGCAGCAGCATAGTATCACAGAGGAGATCGGACACGCGCAGGAGGCTAACCAGACCCTGGTCGGAATT
  GACTGGCAGCATTTATAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001177374
• Insert Size: 5268 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177374.1, NP_001170845.1
• RefSeq Size: 7696 bp
• RefSeq ORF: 5268 bp
• Locus ID: 224826
• UniProt ID: Q6WKZ8
• Cytogenetics: 17 C
• Gene Summary: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth (By similarity). Required for spermatogenesis, promotes, with Tex19.1, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis (PubMed:28708824). Polyubiquitinates LINE-1 retrotransposon encoded, LIRE1, which induces degradation, inhibiting LINE-1 retranstoposon mobilization (PubMed:28806172).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternative exon in the central coding region, compared to variant 1. The resulting isoform (2) is the same length but differs at an internal segment, compared to isoform 1.