Ppl (NM_008909) Mouse Untagged Clone

CAT#: MC224905

Ppl (untagged) - Mouse periplakin (Ppl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppl
• Synonyms: AW553870
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224905 representing NM_008909
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCGCTCTTCAGGAAGAGAAACAAAGGCAAATACAGCCCAACGGTGCAGACGCGGAGCATCTCCA
  ACAAGGAACTCTCAGATCTGATTGAGCAGCTGCAGAAGAATGCAGACCAGGTGGAAAGGAACATTGTGGA
  CACCGAGGCCAAGATGCAGAGCGACTTGGCCCGGATGCAAGAGGGGCAGCTCCCTGAGCACCGGGACGCG
  GCCCTGCAGAACGTGTCTGACTCAGAAAAGCTGCTGTACGTGCTGGAGGCAGATTCGGCCATCGCCAAGC
  ACATGAAGCACCCACAGGGGGACATGATCGCCGAGGACATCCGCCAGCTGAAAGAGCGCGTGACCAACCT
  GCGGGGGAAACACAAGCAGATGTACAGCCTGGCAGTGAAAGAGGCCGACCCGAGAGTCAACTGGGACACA
  CTGGTGGACGAGAAGCTGGACAAGCTGAGCAGCCAGAGCTTTGGGACTGACCTTCCTCTGGTGGATAGCC
  AGGTGGAGCAACACAACATCTTCCACAATGAAGTCAAAGCCATCGGGCCCCACCTGGCCAAGGACAAGGA
  GCAGAACAGTGAACTCCAAGCCAAGTATCAGAAGTTGCTGACAGCATCACAGGCACGGCAGCAGCACCTG
  AGCTCGCTGCAGGATTACATGCAGCGCTGCACCAATGAGCTGTACTGGCTGGACCAGCAAGCCAAGGGCC
  GCATGCAGTATGACTGGAGCGACCGCAACCTCGACTACCCCAGCCGCCGCCGCCAGTACGAGAATTTCAT
  CAACCGGAACCTGGAGGCCAAAGAAGAAAGAATCAACAAACTGCACACTGAAGGTGACCAACTGCTGACA
  GCTGAGCACCCAGGGAGGAACTCCATTGAGGCACACATGGAGGCTGTGCATGCTGAGTGGAAGGAGTACC
  TGAACCTGCTCATCTGTGAGGAGAGCCATCTGAAATACATGGAAGACTACCACCAATTTCACAAAGACAT
  GAAGGATGCTCAGGAGCTGCTACGCAAGGTGGACTCAGACTTGAACCAGAAATATAGCCCTGACTTCAAA
  GACCGGTATCAGATAGAGCTGTTGCTTCGGGAACTGGATGACCAGGAGAAGGCTCTGGACAAGTATGAGG
  ATGTGGTGCGAGGGCTGCAGAGGCGAGGGCAGCAAGTGGTGCCACTCAAGTACCGCCGCGAGACGCCGCT
  CAAGCCCATCCCTGTGGAGGCTCTCTGTGACTTTGAGAGTGACCAGGGCCTCATCTCGCGGGGCTATAGC
  TACACACTGCAGAAGAACAACGGGGAGAGCTGGGAGCTGACAGACAGCACTGGGAAGAAGCTGGCTGCCC
  CAGCCGTCTGCTTCATCATTCCCCCCACCGACCCCGAGGCCTTGGCTCTTGCTGACAGCCTGGGCAGCCA
  GTACCGAAGTGTGAGGCAGAAGGCGACTGGGAGCAAACATGCACTCCAGCAGCGGCATGAGGTGCTAAGG
  ACGGAGAACCCTGGAGATGCCTCTGATCTTCAGGGACGACAGCTGCTGGCTGGCTTGGACAAAGTGGCCA
  GTGACCTGGACCGGCAGGAAAAGGCTATCACAGGGATCCTACGGCCACCACTTGAGCAGGGACGGGCAAT
  TGAGGATAGTGCGGAGCGGGCCAAGGGTCTCAAGAACATTACCAATGAGCTGCTACAGATTGAGCCAGAG
  AAGACGCAGTGCACAGCCGAGTGTGAAGCCTTTGTGCAGGCCCTCCCGGCCAGCGGCACCGCACCCTTGC
  TGAAGACCCGGGTAGAAGACACCAACCAGAAATATGAGCGACTAGTATGGTTGCTAGAGGCAGCCCAGGA
  GAAGGTGGACGTGGCCAACCGCCTGGAGAACAGTCTGCAGCGGGGCAGGGAGCTGCTGGCCTCATACGAG
  AACAGGCTGATTCAGGACGACACAATGCCTGAAAGTGGTCATGTGTTGGACAGCAAGAGGCAGGAGCTGG
  AGGCCATGGCTTCTGAGCTGCAGGCCCACAAGTCCCTCCTGGGTGAGGTGGAACAGAACCTACAGGTGGC
  CAAGCAGTGTTCCAGCTCACTGGCCAGCCGCTTCCAAGAGCACTGCCCTGACCTGGAGCGGCAGGAGGCC
  GAGGTGCACAAGCTCAACCAACGTTTCAACAACCTCAGCCAGCAGGTGGAACGGAGGGCCCAGAGCCTGC
  AGAGTGCCAGGGCTGCCTACGATGAATACTGCAGTGGTTACAACCGTGTGCTCCAGTTTTTGGCCAAGAC
  TCCCAGTTATGAGCCCCAGGAGACAGACAGCCTCGGCCAGATGGAGACCAAGCTCAAGAACCAGAAGAAC
  TTGCTTGATGAGCTAGCAAGCAGGGAACAGGAGGTGCAGAAGGTCTATGCAGATTCCCAGCAGTACCAGC
  AAGCGGTGAAGGACTATGAACTTGAAGCAGAGAAGCTAAGGTCCTTGCTGGACCTGGAGAATGGACGGAA
  CAGCCATGTGAACAAGAGAGCCAGGCTGCAGTCTCCTGCTGCCAAAGTGAAGGAAGAGGAAGCTGCTCTT
  GCTGCCAAGTTCACAGAGGTTAATGCCATCAACAGACAGAGGCTACAGAATTTGGAGTTTGCACTGAATC
  TCTTGAGACAGCAACCAGAAGCAGGAGTGACCCATGAGACCCTACAAGGGGGAAAGCTAAGCTCTGGCAT
  GGAGGAAACATGGAAGATTAAGAAGGAACTGGAGGAGGAGATAGAGCGAAGGCAACAGCTGGAAAATGAG
  GTCAAGAGTGCCCAGGAGGAAATCCAGACCCTGAAGGATCAGGGGCCTCAGGAGTCATTGGTGAGGAAGG
  AGGTGCTGAAGAAGGTGCCAGACCCTGCCCTGGAGGAGAGCTTCCAGCAGCTGCAGCAGACGCTGGCTGA
  GGAACAGCACAAGAACCAGCTATTGCAGGAGGAGCTGGGAGCACTGCAGCTCCGGCTGCAGGCTCTAGAG
  CAGGAGACCAGGGATGGGGGACAGGAGTATGTGGTCAAGGAGGTCTTGCGCATTGAGCCAGACAGAGCCC
  AGGAGGATGAGGTCTTGCAGCTTCGGGAGGAGCTGGAGGCACTGCGGCGCCAGAAAGGCGCCCGAGAGGC
  TGAAGTGCTACTCTTACAGCAGCGTGTAGCAGCCCTGGCTGCTGAGAAGAGCAGGGTGCAGGAAAAGGTC
  ACTGAGAGAGAGGTGGTAAAGCTACAGAATGACCCCCAACTGGAGGCAGAATACCGGAGGCTGCAGGAGG
  AGCACCAGCGGGAGGGCACACTCAGGGAGAAGCAGGAGGAGGAGCTGAGTTTCCTACAGGCTAAGCTCAG
  GAGACTGGAGAAAGAACGAGCCATGGCAGAAGGCAAGATCACTGTCAAAGAGGTGCTCAAGGTAGAGAAA
  GATGCAGCTGTGGAGAGAGAAGTCAATGACCTCACCCGCCAGTATGAGGACGAGGCTGCCAAGGCACGCT
  CTGGCCAGCGGGAGAAGACAGAACTTCTCAGAAAGATATGGGCCCTGGAAGAGGAAAATGCTAAGGTGGT
  GGTACAGGAGAAGGTCCGAGAGATCGTGCGGCCAGATCCCAAGGCAGAGAGTGAAGTGGCCAACCTCCGC
  CTGGAGCTGGTGGAGCAGGAGCGCAAATTCAGGGGTGCTGAGGAGCAGCTGAAGAGCTACCAGAGTGAGC
  TGGAGGCCCTACGGAACCGTGGACCACAGGTAGAAGTCAAAGAGGTGACCAAAGAGGTCATTAAATATAC
  AACTGACCCAGAGACAGAGCAGGAACTCCAGAGGCTCAGGGAGGAGATCATGGACAAGACCAGGTTAATA
  GAAAGGTGTGACCTAGAGATTTACCAGCTGAAGCAGGAGATCCAGGCCCTTAAGGACACCAAGCCACAGG
  TGCAGACTAGAGAGGTAGTCCAGGAGATCCTGCAGTTCCAGGAAGACCCCCAAACCAAGAAGGAGGTAGA
  GTCTCTGCGTATACAGCTGTCAGAAGAACAGAAGAAACAGGTGGACCTGGAAGGGGAGCGGGCATCCCAG
  GAAGAGAAGATCAAACGCAAGGAGGAAGAGCTGGCACAGGGGAAGGAAAGGGTTGTACGCCAGGAGGTAG
  TACAGTATGAAGATGAGCCAGATTTGCGAGCTGAGGTGACTGCCTTCACAAACAGCATTGATGCTGAGCT
  GCGGCAGATTGATAAACTGCATGTGGAGCTACGGCGGTTACAGCATCGCCGAGCAGAGCTGGAGAGGCAG
  TTGGAGGAGCTGGAGCGGGAGAGGCAGGCACGTAGGGCAGCCGAGCTGGAGGTACAGAGGCTGCAACAGC
  GGCTGGCTGCATTGGAGCAGGAGGAGGCCAAGACTGGTGAGAAGGTGACCCACACACAAAAGGTGGTGCT
  GCAGCAGGACCCACAGCAGACCAGGGAGCATGCCCTGCTCCGAGCCCAGCTGGAGGAAGAGCGGCACCGG
  AGGCAGCTACTGGAGGGTGAGCTTGAGCCGCTGCGTAGAAAGTTGGCTGCCCTGGAGAAGGCAGAGATCA
  AAGAAAAGGTGGTCTTCTCTGAGAGTGTCCAGGTAGAAAAAGGTGACACTGAGCAAGAGATCCAGCGCCT
  CAAGAAGAGCCTGGAAGAGGAGAGCCAGAGCAAGAGGGAGCTGGATTCAGAGGTGACCCGGCTAGAAGCC
  AAGTTATCTGAGCTAGAATTCTATAATTCCAAGTCATCCAAGGAACTGGACTTCCTGAGAGAAGAAAACC
  ACAAGCTACAGCTAGAGCGGCAAAACCTGCAGCTAGAGACCCGGAGACTCCAATCAGAGATTGAGATGGC
  AGCAACAGAAACTCGAGATCTGAAGAACATAACCACGATAGACTCTGGGACCCACCTAAACTCTAGGCTG
  TGGTCCCTAGAGAAGGAACTGGATGACCTCAAGAAGATGTCCAAGGACAAGGATCTGGAGATCGATGAAC
  TACAGAGGCGCCTGGGCTCTGTGGCTGTCAAGAGAGAGCAGAGGGAGAACCACCTGAGGCGCTCCATAGT
  GGTCATAGACCCTGACACAGGTCGTGAGCTGTCCCCAGAGGAGGCTCACCGGGCTGGGCTTATCGACTGG
  AAAATGTTTGTGAAGCTCAGAAGCCAGGAGTGCGACTGGGAGGAGATATCAGTGAAAGGTCCTAATGGGG
  AGTCATCAGTAATTCATGACAGGAAGTCAGGCAAGAAGTTCTCCATTGAGGACGCTCTTCAGAGTGGCAG
  GCTAACCGCTGCTCAATATGACCGCTATGTCAACAAGGATATGTCCATTCAGGAGCTGGCAGTCTTGGTG
  TCTGGGCAGAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008909
• Insert Size: 5265 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008909.2, NP_032935.2
• RefSeq Size: 6277 bp
• RefSeq ORF: 5265 bp
• Locus ID: 19041
• UniProt ID: Q9R269
• Cytogenetics: 16 2.5 cM
• Gene Summary: Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.[UniProtKB/Swiss-Prot Function]