Col24a1 (NM_027770) Mouse Untagged Clone

CAT#: MC224882

Col24a1 (untagged) - Mouse collagen, type XXIV, alpha 1 (Col24a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Col24a1
• Synonyms: 5430404K19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224882 representing NM_027770
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTTAGGAGCCTACAGGACAAGGCATGGAAAGGTCTCCCCCACCACAGAAACGAAACTGTTTCTTC
  GCTTTATTGTACTATGTGTGGTGTGGATTTCTGTTCATGCACAGGGACAAGGCATAGATATTCTTCAGCA
  ACTAGGCCTTGGAGGCAGAGATGTAAGATACACGTCATCGGTGACAGCTGTGCCGTCATCGTCATGGTCC
  ACACCATTACCTCAGGGGGTCCATTTAACAGACTTTGGTGTCATCTTAACAGATAATGCCTATATTGAGT
  CACCTCTCGTGAACATTTTACCCATTAGCCTAAGACAGCCACTGACAGTATTGATTGGGTTGCAATCATT
  CAAAGTGAACAATGCATTTCTCTTCAGCATTCGGAATAACAACAGACTGCAGTTTGGCGTACAGCTACTA
  CCTAAGAAACTAATAGTTCATGTCGGAGGAAAGCAGACAGTAACCTTCAACTACAGTGCCCATGATGAGC
  GATGGCACTCATTCGCCATCACTGTTGACCACCATGTCATCTCCATGTTTGTTGAGTGTGGAAAGAGACA
  TTTTAGTGGAGAGACGACTTCAGACGTTCAGACCTTTGACCCTCACAGTGTGTTTACCTTGGGAAGTATA
  AATAACAGCTCTGCCCACTTTGAAGGAACAGTGTGCCAGTTGGAGATCATGCCTTCCACAGCGGCATCTG
  CGGAGTATTGCAGACACCTGAAACAGCAGTGCCTCAGAGCAGACGCATCCCAAGCTCAAAGAAACCTTCC
  TCACACGGCAGGAATGCCCACCAGACACCCTGCGCACACTCCATTACCCAGAGGATTTCCTGGAACAGAT
  TCACCACAGAAGAGATTCACAGAGCAGGATTCCTTGCCAAAAGGATTTGATGGAACAGAACTGCCACGGG
  AGACGTTCGCTGATGGCAAAAGCATTCCAAACAACAGAAGTAATGGTTCTGCCACGGTACATGAAAGCCA
  AGAGCACCAGACACCAAGGGCTCAGTTAACTTCTTTCCATTCAGGAAACATCTCCGCTGTGACTCTCCCA
  AACTACAGAATTCAGGCTAAAGAAATCACCACCAAGGAAGAAACAAATTTAACCCTGTCAGTGGCCCATC
  ACCTCCCTAGTGAGGCAAGAATGAATGAAGAGGGGAGAATTAACCCTCTCTTCGCTGGATTCGACAACAT
  CACCCAACACGAGGAGGCAGCTGGCCTGCCCCTGCCTAAGAAAGCATCATCCGGCTTTGCACACACAAAT
  CAAGACACCATGAAGAACCTCGAGAAGGCTCTCACGGCAAATCTATACACCAATGAGCTCATAGAAATGG
  AACGGATCTTAAACAGCACCTTGTATAGAGTTATGTACGGGCCCTCGGTGGATAACCACCTTGAACTAAG
  GAAGGAAGGTGAATTTTACCCTGATGCCACGAATCCCATCGAAGGCAGCTATGAGCCCCAGGCTTATGAT
  TATTACTCTTATGAGGATTACAACGCAGTGCTTGACATGGAGTACCTGAGAGGGCCGAAGGGAGACCCGG
  GACCTCCTGGTCCCCCTGGTCCGATGGGTATCCCAGGTCCGTCAGGCAAGAGAGGCCCGCGGGGTATTCC
  AGGACCACATGGAAATCCCGGGTTGCCTGGACTGCCAGGTCCAAAGGGCCCCAAAGGAGATCCTGGGCTG
  TCCCCAGGCCAAGCTGCTTCTGGAGAAAAGGGTGACCCAGGTCTTTTGGGGTTAGTGGGCCCCCCTGGCT
  TACAAGGTGCAAAGGGTCTCAAAGGACATCCAGGGCTCCCCGGACTCCGCGGCGAACACGGACTTCCAGG
  ACTCGCTGGTAATATTGGCTCACCTGGCTACCCTGGCAGGCAGGGTTTAGCTGGACCAGAGGGTAACCCA
  GGCTCCAAAGGTGTCCGAGGTTTCATCGGTTCTCCTGGAGAGGTGGGACAGCTGGGACCTGAAGGAGAAA
  GGGGCACCCCTGGGGTCCGTGGAAAGAAGGGTCCGAAGGGGAGACAGGGTTTCCCAGGTGACTTTGGAGA
  CAGAGGCCCTGCTGGTCTGGATGGCAGTCCTGGGCTTGTAGGTGGCACCGGACCTCCCGGGTTTCCTGGT
  GTTACAGGAAGTGTTGGTCCTGCAGGACCGACTGGACCTCCAGGAGCTCCTGGCCCAATGGGCCTTTCAG
  GAAGTAGAGGACCATCTGGAATCAAAGGTGATAAGGGTGAGCAAGGTGTGGCAGGGGAGCCAGGAGAACC
  CGGGTATCCTGGAGACAAGGGCAACATTGGATCACCAGGACCACCAGGGATAAGAGGAAAGTCAGGACCT
  TCAGGACAACCAGGTGACCCAGGACCACAAGGACCATCTGGCCCTCCAGGACCAGAGGGTTTTCCGGGAG
  ACATTGGGATTCCTGGACAAAATGGACCTGAAGGACCAAAGGGACATCTTGGAAACAGAGGGCCCCCTGG
  TCCTCCAGGTCTCAAAGGAACTCAGGGAGAAGAAGGACCAATTGGACCCTTTGGAGAACTGGGATCTAGG
  GGAAAACCAGGTCGAAAAGGGTATATGGGTGAACCAGGGCCAGAAGGCTTAAAGGGAGAAGTGGGAGACC
  AAGGAGATATTGGGAAAACTGGTGAAACAGGGCCCGTTGGTTTACCTGGAGAAGTAGGGATAACCGGAAG
  CATTGGTGAGAAGGGAGAACGTGGAAGTCCAGGCCCACTTGGTCCCCAGGGGGAAAAAGGTGTCATGGGC
  TACCCAGGTCCTCCTGGGGCTCCAGGGCCCATGGGCCCACTTGGATTACCTGGTCTTGTTGGGGCAAGAG
  GCGCACCTGGGAGTCCGGGTCCTAAAGGACAAAGAGGACCCAGAGGACCAGATGGCCTTGCAGGGGATCA
  AGGAGGACATGGTGCTAAGGGTGAAAAAGGAAACCAAGGGAAAAGAGGGCTTCCTGGCCTCCCCGGTAAG
  GCTGGAAGCCCTGGGGAGAGGGGAGTTCAAGGAAAACCGGGCTTCCAAGGACTGCCTGGCAGCTCAGGAG
  ACGTGGGGCCTGCCGGAGAACCCGGACCACGAGGACTGCCTGGTATTGCTGGACTTCCTGGAGAGATGGG
  TGTTGAGGGACCTCCCGGCACTGAGGGAGACTCTGGCCTGCAAGGTGAACCAGGTGCTAAGGGAGATGGT
  GGACCTGCCGGCAGTGCTGGAGCCACTGGGGAGCCGGGGCCGCGAGGAGAACCAGGAGCCCCTGGAGAAG
  AAGGTCTCCAAGGAAAAGATGGGTTAAAGGGGGCTCCAGGAGGAAGCGGACTTCCTGGAGAGGATGGAGA
  CAAAGGAGAGATGGGCTTACCAGGAACGGCAGGGCCCGTGGGGAGACCAGGTCAGATGGGCCTTCCAGGA
  CCTGAAGGAATTGTGGGAACCCCGGGACAAAGAGGTCGACTAGGGAAAAAGGGTGATAAAGGACAAGTAG
  GACCCACGGGAGAAGCTGGAAGCAGAGGACCCCCTGGAAGCGTTGGGGAAAATGGCCCTAAGGGTGCCAG
  GGGAACTCGAGGTGCTGTGGGTCCATTAGGATTGATGGGACCTGAAGGCGAACCAGGAATCCCAGGATAC
  AGAGGTCATCAAGGTCAACCAGGACCCTCTGGATTGCCAGGACCTAAAGGAGAAAAGGGCTACCCAGGAG
  AAGATAGCACAGTTCTGGGACCTCCTGGGCCTCCAGGTGAACCAGGGCCAATGGGGGAGCAAGGAGAGAC
  AGGGGAACATGGCGAAGAAGGCTATAAGGGCCATATGGGTGTGCCAGGACTCAGGGGTGCCACTGGACAG
  CAAGGACCCCCAGGTGAACCAGGTGACCAGGGTGGACAAGGACCGAAAGGAGAGAGAGGATCTGAAGGTC
  CTCAGGGGAAAAGGGGAGTTCCTGGTCCTTCTGGGAAGCCAGGCATTCCCGGAGTTCCAGGCTTTCCTGG
  GCCAAAAGGCTTACAAGGATACCCTGGAGTCGACGGCATGTCAGGATACCCTGGGAAGCCTGGTCTACCA
  GGAAAACAAGGACTTTTGGGTGTCCCGGGCAGCCCAGGGAGAACAGGAGTGGCTGGATCCCCAGGTCCTC
  AAGGAGGAAAAGGCGCATCAGGCCCTCCAGGAAGCCCTGGTGCTCCAGGCCCAAAGGGTGAACAAGGGCT
  GCCTGGTCAGCCTGGAGTCCCGGGTCAAAGAGGTCATCGAGGAACACCAGGCGACCAAGGACTCCGTGGA
  GCGCCTGGTCTGAAAGGGCAGCCTGGGGAACATGGTGATCAAGGTTTGGCAGGTTTCCAGGGATTCCCAG
  GCCCCAGAGGTCCCGAGGGAGATGCTGGCATTGTTGGAATTGTAGGCCCTAAAGGTCCCATTGGGCAGAG
  AGGAAACACTGGCCCTCTTGGCCGAGAAGGGATAATAGGCCCAACGGGTGGAACTGGACCCAGAGGTGAA
  AAAGGCTTTAGAGGTGAAACTGGTCCCCAAGGACCGAGAGGTCAACCAGGACCCCCTGGTCCACCTGGAG
  CACCTGGTCCAAGAAGACAAATGGATATCAATGCTGCTATTCGAGCTCTGATAGAATCAAATTCTGCCCA
  GCAGATGGAGAGTTACCAGAACACTGAAGGGACCTTAATCAGCCACAGTTCAGACATATTCAAAACCCTG
  ACCTACCTTAGCAGTCTCCTGAGCAGCATCAAGAATCCTCTTGGCACCCGAGAAAACCCAGCACGGATCT
  GCAAGGATTTGCTCAGCTGTCAGTACAAGGTTTCAGATGGAAAATACTGGATTGACCCAAATCTCGGATG
  CTCTTCAGATGCCTTTGAGGTTTTCTGCAACTTTAGTGCTGGAGGCCAGACATGTTTATCTCCCGTTTCT
  GTGACAAAGTTGGAGTTTGGAGTCAGCAAAGTACAGATGAACTTTCTTCACTTGCTGAGCTCAGAAGCCA
  CCCACACCATCACCATTCACTGTCTAAACACCCCAAGGTGGAGCAGCACATGGGCGGATGGTCCAGAGTT
  GCCTATTAGTTTCAAAGGATGGAATGGTCAGATTTTTGAAGAAAATACTCTACTTGAACCACAAGTCCTC
  TCAGATGACTGCAAGATTCAGGATGGTAGCTGGCACAAGGCGAAATTCCTTTTTCACACCCAGAATCCTA
  ATCAGCTTCCTGTGACTGAAGTCCAAAACCTTCCTCACCTTGGAACTGAGCAAAAGCGCTACATTGAAAG
  CAATTCCGTGTGCTTTCTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027770
• Insert Size: 5202 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027770.3, NP_082046.2
• RefSeq Size: 7143 bp
• RefSeq ORF: 5202 bp
• Locus ID: 71355
• UniProt ID: Q30D77
• Cytogenetics: 3 H2
• Gene Summary: This gene encodes the alpha-1 subunit of type XXIV collagen, one of the low abundance fibril-forming collagens found in cartilage. The encoded protein has structural features of invertebrate fibrillar collagens and is expressed predominantly in bone tissue. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.